##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513022_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1732075
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.80966124446112	31.0	29.0	34.0	22.0	34.0
2	28.54588167371505	31.0	28.0	34.0	16.0	34.0
3	28.896811050329806	31.0	29.0	34.0	18.0	34.0
4	31.735402912691423	35.0	32.0	37.0	17.0	37.0
5	31.979410822279636	35.0	32.0	37.0	19.0	37.0
6	32.43294314622635	35.0	33.0	37.0	24.0	37.0
7	32.41537693229219	35.0	33.0	37.0	23.0	37.0
8	32.38375936376889	36.0	33.0	37.0	23.0	37.0
9	33.69370437192385	38.0	34.0	39.0	22.0	39.0
10	33.182714374377554	38.0	33.0	39.0	17.0	39.0
11	33.55623803819119	38.0	34.0	39.0	21.0	39.0
12	33.423442980240466	38.0	33.0	39.0	18.0	39.0
13	33.470574888500785	38.0	34.0	39.0	18.0	39.0
14	33.75446444293694	39.0	33.0	41.0	10.0	41.0
15	34.25551664910584	39.0	33.0	41.0	17.0	41.0
16	33.817853730352326	39.0	33.0	41.0	11.0	41.0
17	33.34263585583765	39.0	32.0	41.0	10.0	41.0
18	32.71726455263196	38.0	31.0	41.0	10.0	41.0
19	33.63807283171918	39.0	32.0	41.0	11.0	41.0
20	33.912339823621956	39.0	33.0	41.0	10.0	41.0
21	33.93591732459622	39.0	34.0	41.0	10.0	41.0
22	33.49613613729198	39.0	33.0	41.0	2.0	41.0
23	31.748195083931122	38.0	30.0	41.0	2.0	41.0
24	32.832235902025026	38.0	32.0	41.0	2.0	41.0
25	32.55764444380295	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8895599311251488
1101	2	1.2572521429909216
1101	3	1.008577182528441
1101	4	1.0591653421737384
1101	5	1.3031339337042454
1101	6	1.0021703698690771
1101	7	1.0537258630388635
1101	8	1.0278819272521744
1101	9	1.239686907408327
1101	10	1.5729047408881272
1101	11	1.2295664008436589
1101	12	1.2054888364139842
1101	13	1.1851521398619838
1101	14	0.11877530115208401
1101	15	0.7518346215257239
1101	16	1.5184444833057427
1101	17	2.1254704203216903
1101	18	-3.9789918213042377
1101	19	-0.4013408917499248
1101	20	0.5790036778213903
1101	21	0.9972253857891573
1101	22	1.6258724182472335
1101	23	3.4001935720894316
1101	24	2.455833178588577
1101	25	2.597141019233568
1102	1	1.0252905594509976
1102	2	1.3775682572792078
1102	3	1.1800330721018
1102	4	1.6680057815850553
1102	5	1.524236490620119
1102	6	1.1699570331432199
1102	7	1.0766864892245067
1102	8	0.9014142135414076
1102	9	1.1073647051304363
1102	10	1.5968969062789498
1102	11	1.202585369158676
1102	12	1.262796511137232
1102	13	1.17691678883849
1102	14	2.120440288122012
1102	15	1.4611564414821103
1102	16	1.7805786854431673
1102	17	1.3415471390622713
1102	18	1.7015926734052158
1102	19	1.5531966416866467
1102	20	1.4771625049093657
1102	21	1.4684229267396347
1102	22	1.9061599346217903
1102	23	3.560732824316272
1102	24	2.356837352320241
1102	25	2.722759011800491
1103	1	0.8942472237808765
1103	2	1.1733889656167875
1103	3	0.9024383066785155
1103	4	1.474336885412587
1103	5	1.3066998095165374
1103	6	0.9325289710623252
1103	7	0.8802628927036622
1103	8	0.7905462594493002
1103	9	0.8579768424511869
1103	10	1.1775950704669
1103	11	0.9450075040125512
1103	12	1.069347405587493
1103	13	0.8541494469034845
1103	14	1.788590332732781
1103	15	1.374429725081498
1103	16	1.5840929885124169
1103	17	1.8942207462957654
1103	18	1.9797771949505645
1103	19	1.3466050589423304
1103	20	1.261183871669509
1103	21	1.2546110835981494
1103	22	1.5190534036759473
1103	23	3.0977754551269605
1103	24	2.0107486035828472
1103	25	2.1631553416267124
1104	1	0.26427482777518563
1104	2	0.7651538615305569
1104	3	0.5119271468905247
1104	4	0.9622935594858468
1104	5	0.82579371037329
1104	6	0.3349315166181057
1104	7	0.29138722361540914
1104	8	0.24555437518121437
1104	9	0.44110846438940143
1104	10	0.8328972173274991
1104	11	0.34917121592245337
1104	12	0.43692742040067856
1104	13	0.363812946070901
1104	14	1.2427477411615868
1104	15	0.659704661915363
1104	16	1.0788705544231902
1104	17	1.4078910269582607
1104	18	2.0769775300856423
1104	19	1.2868253393421796
1104	20	0.6676431012790616
1104	21	0.8661412011055418
1104	22	1.1172361626045628
1104	23	2.5543453896232897
1104	24	1.6046472278480977
1104	25	1.7063672804199115
1105	1	0.795211113470252
1105	2	1.2688501346007328
1105	3	0.9406197980658284
1105	4	1.2411836039316704
1105	5	1.3610964490432238
1105	6	1.036957061325225
1105	7	0.9307921462879492
1105	8	0.8521459651413252
1105	9	1.1288149770737945
1105	10	1.481638220799347
1105	11	1.1830144603123998
1105	12	1.037171510244363
1105	13	1.083779158965882
1105	14	1.79070628598771
1105	15	1.1952332516012447
1105	16	1.5521813285256059
1105	17	2.056540965644757
1105	18	1.8478467589842182
1105	19	1.5134507940193416
1105	20	1.2685933312693862
1105	21	1.2455326192843543
1105	22	1.7401692248131937
1105	23	3.3075529655925315
1105	24	2.1980847424003542
1105	25	2.1831938796158497
1106	1	-0.6744248810970532
1106	2	-0.16618254216660588
1106	3	-0.3715899310139932
1106	4	-2.8457402940593894
1106	5	-0.7303211657781006
1106	6	-0.553166846384805
1106	7	-0.6302683865597487
1106	8	-0.6662347083231346
1106	9	-0.5831227515064938
1106	10	-0.44643788186274946
1106	11	-0.5016767135705393
1106	12	-0.44749923290275007
1106	13	-0.5236232205878153
1106	14	0.2403519028568084
1106	15	-0.33297220217728807
1106	16	-1.175964870893786
1106	17	-6.099917321885975
1106	18	-7.102950960663499
1106	19	-3.3391641768766576
1106	20	-1.8436512241158738
1106	21	-1.418422664052983
1106	22	-1.8214496262244033
1106	23	-5.6729740873012595
1106	24	-2.7591618527926975
1106	25	-1.2349012730091893
1107	1	0.6146394866728606
1107	2	0.9841500550376772
1107	3	0.7281640230703026
1107	4	1.0467415400165727
1107	5	1.137907795956977
1107	6	0.6062529468610052
1107	7	0.5852102528973191
1107	8	0.5365194147281827
1107	9	0.49859820713912484
1107	10	1.130501388869284
1107	11	0.7396869645067241
1107	12	0.8331018170559545
1107	13	0.7833906437667224
1107	14	1.560848862828955
1107	15	0.9779629574177733
1107	16	-5.141271097033105
1107	17	-0.723490308746328
1107	18	0.8885112815091745
1107	19	0.8289241365528355
1107	20	0.7987401200463182
1107	21	0.9058660103908878
1107	22	0.8012793132574387
1107	23	2.7858718088679133
1107	24	1.9147621246515811
1107	25	1.445688039846445
1108	1	0.31406666485230517
1108	2	0.6026058844337214
1108	3	0.4588587100525032
1108	4	0.9583463290489682
1108	5	0.7544189961385115
1108	6	0.38301924916283525
1108	7	0.30302547106187205
1108	8	0.3325549414682598
1108	9	0.4191983991615231
1108	10	0.8549357458201285
1108	11	0.3380824766452051
1108	12	0.4063966835983095
1108	13	0.17265751076394764
1108	14	0.8277988118635733
1108	15	0.5563378756992137
1108	16	0.6998420513086145
1108	17	1.14735022283066
1108	18	0.6589973920793923
1108	19	0.6639132606492595
1108	20	0.5141279268836243
1108	21	0.5730320754946874
1108	22	1.1011525618579014
1108	23	2.7351361989942866
1108	24	1.5103148173529135
1108	25	1.554010897106508
1109	1	0.2234808430471169
1109	2	0.5842866338540738
1109	3	0.4816014495241525
1109	4	0.3928218437308182
1109	5	0.21160094116649475
1109	6	0.2148091853106422
1109	7	0.22714380768896092
1109	8	0.18355499852545876
1109	9	0.29516020196147963
1109	10	0.8767533806436916
1109	11	0.43756922122088326
1109	12	0.5131273580662494
1109	13	0.38239639628127975
1109	14	-0.7918011134433698
1109	15	0.022861121320069344
1109	16	-0.17928746334212065
1109	17	0.7503602493363175
1109	18	1.7362147125696694
1109	19	0.7499194643227014
1109	20	0.5617758736025777
1109	21	0.42002931081412953
1109	22	0.30239277784532703
1109	23	-4.64949207336565
1109	24	-1.6179373132147
1109	25	-0.3199222629804268
1110	1	0.5057741914078164
1110	2	0.8280002711869194
1110	3	0.5590593881353421
1110	4	1.1586241831258697
1110	5	0.9470843044530142
1110	6	0.5047728353355154
1110	7	0.366702328102825
1110	8	0.33957457471317554
1110	9	0.5115933352684081
1110	10	0.9460813122488787
1110	11	0.5473339617450108
1110	12	0.5338851738185824
1110	13	0.41138448607993183
1110	14	1.2648081344776756
1110	15	0.740691647176277
1110	16	1.1174128625892195
1110	17	1.50733985070503
1110	18	2.147886303744137
1110	19	1.3726384526155755
1110	20	0.8549465458740428
1110	21	0.8635002765253432
1110	22	1.1621082732914303
1110	23	2.613939140906286
1110	24	1.7531045501784348
1110	25	2.1044520199198544
1111	1	0.8732339609150017
1111	2	1.3303595483497475
1111	3	1.019490845299753
1111	4	1.5007300483483768
1111	5	1.2589171432727753
1111	6	0.7961462340978471
1111	7	0.7837033591585438
1111	8	0.59499880228568
1111	9	0.6802914393053925
1111	10	1.0168326813973323
1111	11	0.7782019805114473
1111	12	0.9006692662760187
1111	13	0.8829089436571707
1111	14	0.13633264289592972
1111	15	0.5979359321381139
1111	16	0.9031253688680891
1111	17	1.5796871192208144
1111	18	2.2505485424975973
1111	19	1.3502717119921854
1111	20	1.0674802788855189
1111	21	0.9897957055116322
1111	22	1.3674307171578661
1111	23	1.4479518796302564
1111	24	1.34718991020398
1111	25	0.8771372794835557
1112	1	0.01855834618913832
1112	2	0.42935017246599827
1112	3	0.21248866266942912
1112	4	0.6615250043694623
1112	5	0.28977670805193867
1112	6	-0.26039911892991796
1112	7	-0.20698783128138842
1112	8	-0.23770367112030755
1112	9	-0.2943454536732091
1112	10	0.08569067990357127
1112	11	-0.20203651354614038
1112	12	-0.07323162345402068
1112	13	-0.24551637195406784
1112	14	-0.032056198859748974
1112	15	-0.23319482989717244
1112	16	0.22140145347582774
1112	17	0.2601982607872344
1112	18	0.39952756459219785
1112	19	-0.15011079215916823
1112	20	-0.09099608562100059
1112	21	-0.10119677492808421
1112	22	-0.08383354063905557
1112	23	1.5489764831674258
1112	24	0.4411535958762087
1112	25	0.5511222838705692
1113	1	0.3556457660174033
1113	2	0.8817572866221361
1113	3	0.41051393597021146
1113	4	0.5429692010465068
1113	5	0.643263970088789
1113	6	0.18459985255323375
1113	7	0.23467820698969888
1113	8	0.11916679577504397
1113	9	0.29663199128575
1113	10	0.6323522578484742
1113	11	0.3629099651045635
1113	12	0.25353702796802935
1113	13	0.16067199077534866
1113	14	0.6953163022418707
1113	15	0.31093046653882794
1113	16	0.6426242445884185
1113	17	1.0945117629820018
1113	18	1.6782265189932488
1113	19	0.7367332462134897
1113	20	0.43277419552575225
1113	21	0.3578633812525851
1113	22	0.678725778621299
1113	23	0.45653231162620145
1113	24	0.3533191547712349
1113	25	0.8436769760875436
1114	1	-0.7372021155713249
1114	2	-3.299498312382486
1114	3	-1.3345445263637572
1114	4	-0.3343335814891226
1114	5	-0.14395200767678062
1114	6	-0.3088089313397333
1114	7	-0.47331106360461916
1114	8	-0.3677815931982309
1114	9	-0.2703829717107311
1114	10	-0.18338868517579243
1114	11	-0.32860217011180026
1114	12	-0.2843571645795606
1114	13	-0.3720473964850939
1114	14	0.49031032350677606
1114	15	-0.13038097708331975
1114	16	0.24908399315837215
1114	17	0.49655901114798695
1114	18	0.720338531403165
1114	19	0.1396698716214999
1114	20	-0.0306151332400475
1114	21	-0.17317368690499535
1114	22	-0.1107137715192863
1114	23	1.5835219377128773
1114	24	0.47495157567419
1114	25	0.4397427744910587
1115	1	0.010010487187248174
1115	2	0.2507824291990488
1115	3	0.11169026192778375
1115	4	0.1878409447035665
1115	5	0.011163185113780116
1115	6	-0.2204029188883183
1115	7	-0.4502576463446175
1115	8	-0.33446256867375723
1115	9	-0.3896281820433387
1115	10	-0.07951251140631399
1115	11	-0.0977673749410215
1115	12	-0.31333043875147126
1115	13	-0.3088720791803823
1115	14	0.5532381259351453
1115	15	-0.08702459831263809
1115	16	0.5050216672890073
1115	17	0.8504534148902891
1115	18	1.1649231287959338
1115	19	0.5494332947288498
1115	20	-0.037335595524638165
1115	21	0.027035441839885266
1115	22	0.33207190591338787
1115	23	2.056371660823668
1115	24	0.7036795363249624
1115	25	0.2877356200866288
1116	1	-0.4714871493148749
1116	2	-0.5476380503737523
1116	3	-0.5140395640771374
1116	4	0.34130754594239576
1116	5	-0.43692858568392623
1116	6	-0.5285850329673636
1116	7	-0.7515182886939407
1116	8	-0.6778840147941025
1116	9	-0.7563885294717423
1116	10	-0.29895994361167766
1116	11	-0.7203528648319306
1116	12	-0.6036981729162036
1116	13	-0.6890033313798938
1116	14	-0.17015017669171328
1116	15	-0.8820284562417129
1116	16	-0.5481657091044312
1116	17	-0.1579006950846491
1116	18	0.19724365248295328
1116	19	-0.7161633955480156
1116	20	-0.8384166417751757
1116	21	-1.0052340283103192
1116	22	-0.5533005122894323
1116	23	0.8683492501384471
1116	24	-0.1679130531467763
1116	25	0.12985313574552748
1201	1	1.173052049112158
1201	2	1.547343871611531
1201	3	1.310036693463534
1201	4	1.9008007083048533
1201	5	1.6670479243283864
1201	6	1.23969531833729
1201	7	1.1316585427053028
1201	8	1.196715313036897
1201	9	1.4983364136159878
1201	10	1.8201217520309783
1201	11	1.441340034280664
1201	12	1.5180117753810052
1201	13	1.4191992207719935
1201	14	2.364757865493587
1201	15	1.8607796947056272
1201	16	2.207198088487125
1201	17	2.5658558085913796
1201	18	3.2055817489186325
1201	19	2.2554764943181738
1201	20	2.0112454773535475
1201	21	1.7995005498530077
1201	22	2.087985623326709
1201	23	3.6118717031307064
1201	24	2.607750378965136
1201	25	2.6038730525899254
1202	1	1.1221015391853086
1202	2	1.4830929202889251
1202	3	1.256867715197604
1202	4	1.8169978671297038
1202	5	1.6716586057637315
1202	6	1.2327191861029618
1202	7	1.0127905066123475
1202	8	1.071169733481831
1202	9	1.2802717508615657
1202	10	1.8021536016868396
1202	11	1.3660393367515127
1202	12	1.3889893390479457
1202	13	1.327205575346369
1202	14	2.278024482708382
1202	15	1.6703104633608419
1202	16	1.9940789861758432
1202	17	2.3368218266389746
1202	18	3.000922474997971
1202	19	2.304372892841563
1202	20	1.9282979087573011
1202	21	1.7506466067017357
1202	22	2.1576456412672513
1202	23	3.8145338931609523
1202	24	2.6048219391061878
1202	25	2.687004874698829
1203	1	0.9425012871711829
1203	2	1.306699080541165
1203	3	1.0011344985219708
1203	4	1.6157629141939687
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2211	17	-0.44756637620237427
2211	18	-5.785331560256164
2211	19	-2.2367378656000163
2211	20	-1.4545788609368202
2211	21	-1.1350381876715403
2211	22	-0.7387278629176635
2211	23	-5.1240671936437465
2211	24	-2.65933188324221
2211	25	-1.9899057886429716
2212	1	0.46160635488996604
2212	2	0.6833740911783437
2212	3	0.35944144612073003
2212	4	0.936908662913357
2212	5	0.8671421385330973
2212	6	0.506062123421529
2212	7	0.39090879072502815
2212	8	0.15560146587918666
2212	9	0.0019210477119813163
2212	10	-3.6628861224566123
2212	11	-1.0093642418679352
2212	12	-0.17353574564241114
2212	13	0.030970947189032927
2212	14	0.8924886348097161
2212	15	0.4776915883334638
2212	16	0.679447899281918
2212	17	1.1316137724104607
2212	18	1.6944677464018127
2212	19	0.8304691702457205
2212	20	0.45067539818698776
2212	21	0.37818050786166424
2212	22	0.6466431208465124
2212	23	1.8862100952526681
2212	24	1.0741414866534171
2212	25	0.9789224345935708
2213	1	-1.3703226660543208
2213	2	-3.7814808451335793
2213	3	-1.5364558668427826
2213	4	-3.2867128325146595
2213	5	-1.1305967079546662
2213	6	-0.8003463138989488
2213	7	-0.7590146496837527
2213	8	-0.6901838692336959
2213	9	-0.49168692830726
2213	10	-0.6466309770905738
2213	11	-0.6530311763311971
2213	12	-0.5768422777196918
2213	13	-0.9352915409868814
2213	14	-6.228228779311802
2213	15	-2.758738791030652
2213	16	-2.006680992947228
2213	17	-0.7069545524354481
2213	18	-0.09045649307745407
2213	19	-0.5599042980225377
2213	20	-0.6336859285291823
2213	21	-0.8738657604791626
2213	22	-0.8644358834684738
2213	23	-5.4413305169649036
2213	24	-3.029193984733382
2213	25	-2.0544263402370078
2214	1	0.031077993492981904
2214	2	0.1233605025407023
2214	3	0.05945002042084013
2214	4	0.3638721776383669
2214	5	-0.02266345277886117
2214	6	-0.1760210082167717
2214	7	-0.22199710470324163
2214	8	-0.3899804360525252
2214	9	-0.1738746256310577
2214	10	0.3009132367501195
2214	11	-0.21786598603252827
2214	12	-0.2452177085710474
2214	13	-0.2982943872412278
2214	14	0.2955906320789623
2214	15	-0.031923838026322926
2214	16	0.4119452021761276
2214	17	0.6669898151647473
2214	18	1.2373400609635823
2214	19	0.2422892770880125
2214	20	-0.01741409579906872
2214	21	-9.039522504750153E-4
2214	22	0.4138557569770924
2214	23	1.898680546477923
2214	24	0.8234753503632888
2214	25	0.6263507415057532
2215	1	-2.965236483642279
2215	2	-4.97356233677273
2215	3	-3.201807830133159
2215	4	-4.2774377943494315
2215	5	-5.279575899029329
2215	6	-2.9200580998541454
2215	7	-2.586934400633446
2215	8	-3.0909415155929096
2215	9	-2.554691730025585
2215	10	-4.201055813778897
2215	11	-3.0258000636816433
2215	12	-2.5161379295463
2215	13	-2.6796233388354267
2215	14	-1.999538623278145
2215	15	-2.708055589056709
2215	16	-3.7025385123848658
2215	17	-7.667725356922968
2215	18	-4.165361856623445
2215	19	-3.3161527447864856
2215	20	-2.9075314303575617
2215	21	-2.978667035020294
2215	22	-2.819702685044785
2215	23	-7.245958383794882
2215	24	-4.978057570667275
2215	25	-4.8584789549989615
2216	1	-1.7080315560015045
2216	2	-1.3276794584083653
2216	3	-1.634333133320574
2216	4	-4.004614206594145
2216	5	-2.163265064305918
2216	6	-1.815373206251511
2216	7	-1.87776678533206
2216	8	-1.697128257624378
2216	9	-1.69551185761226
2216	10	-1.5701801487953517
2216	11	-2.1592659241027263
2216	12	-3.042436478980502
2216	13	-2.2486224258919165
2216	14	-7.924115823359298
2216	15	-4.47885182231941
2216	16	-2.8862021772331623
2216	17	-1.9113868802988314
2216	18	-0.985283044802518
2216	19	-1.6491178951403924
2216	20	-2.1724329789802574
2216	21	-2.505443893592947
2216	22	-1.942927517108263
2216	23	-0.9914789024820472
2216	24	-1.7361648753424497
2216	25	-1.6167179884059344
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93887.0
3	1.0
4	793.0
5	4680.0
6	5817.0
7	5118.0
8	4772.0
9	4423.0
10	4126.0
11	4130.0
12	4068.0
13	4032.0
14	4061.0
15	4186.0
16	4286.0
17	4610.0
18	5087.0
19	6121.0
20	7621.0
21	9653.0
22	11816.0
23	14388.0
24	17039.0
25	19608.0
26	22460.0
27	25375.0
28	28300.0
29	34196.0
30	39744.0
31	45652.0
32	58137.0
33	75069.0
34	95445.0
35	123623.0
36	172671.0
37	233780.0
38	533300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04076588917171	18.47868304422913	12.25265464936434	25.227896417234817
2	13.99390486928856	20.158119890158257	41.997079512805435	23.850895727747748
3	19.04180224761152	24.904328078741486	29.34670487767971	26.70716479596728
4	10.776831190372645	16.615410027127034	39.83157790171777	32.77618088078254
5	12.177275431290898	38.096544474798286	36.77555546571331	12.950624628197502
6	31.578953446876035	37.18382992385702	18.4768862798269	12.760330349440045
7	27.811453527753766	31.55693992819818	22.37775240787868	18.253854136169373
8	25.088440229650082	36.632907503589	20.848621972445226	17.43003029431569
9	26.64479060368809	15.027088086959084	19.820244367404666	38.50787694194816
10	13.716259610408812	29.34097083610557	35.664954812597024	21.277814740888594
11	34.26384477680892	22.88392908702431	24.132124501934516	18.720101634232257
12	22.736052747526884	25.186043248306166	32.09622104253514	19.98168296163181
13	28.72858150892887	21.74335574934259	25.757392028400538	23.770670713328
14	20.73003617842634	21.849055405253235	28.668385599080672	28.752522817239758
15	23.704414199399785	29.876416737935457	23.5046690924042	22.914499970260557
16	21.996725732451342	28.301047823362797	28.051695314101583	21.650531130084282
17	21.86806282238746	28.060443101572407	28.261469659484916	21.81002441655521
18	21.456454427548007	27.438751857972914	30.07958533855818	21.0252083759209
19	23.053510942729343	27.35766077683416	28.132993361304987	21.45583491913151
20	23.087957340577212	27.45293702202904	28.267642372762925	21.191463264630826
21	22.934190351614273	26.99717311018518	27.74180880522758	22.32682773297297
22	22.574221285816925	27.73378752070526	27.533319010028624	22.15867218344919
23	21.589506012421012	27.039897619633376	29.174188689880122	22.19640767806549
24	22.344859930217797	27.779577876003458	27.963303409347535	21.91225878443121
25	22.557325885472977	26.963899592493	27.853357543499758	22.62541697853427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	210.0
1	210.0
2	336.0
3	462.0
4	462.0
5	462.0
6	1005.5
7	1549.0
8	1549.0
9	1549.0
10	2446.0
11	3343.0
12	3343.0
13	3343.0
14	5851.0
15	8359.0
16	8359.0
17	8359.0
18	14712.0
19	21065.0
20	21065.0
21	21065.0
22	33435.5
23	45806.0
24	45806.0
25	45806.0
26	63802.0
27	81798.0
28	81798.0
29	81798.0
30	103804.5
31	125811.0
32	125811.0
33	125811.0
34	144647.5
35	163484.0
36	163484.0
37	163484.0
38	178899.5
39	194315.0
40	194315.0
41	194315.0
42	206230.5
43	218146.0
44	218146.0
45	218146.0
46	230660.0
47	243174.0
48	243174.0
49	243174.0
50	230088.0
51	217002.0
52	217002.0
53	217002.0
54	189841.5
55	162681.0
56	162681.0
57	162681.0
58	137423.5
59	112166.0
60	112166.0
61	112166.0
62	90175.0
63	68184.0
64	68184.0
65	68184.0
66	52501.0
67	36818.0
68	36818.0
69	36818.0
70	26821.0
71	16824.0
72	16824.0
73	16824.0
74	11821.0
75	6818.0
76	6818.0
77	6818.0
78	4771.5
79	2725.0
80	2725.0
81	2725.0
82	1815.0
83	905.0
84	905.0
85	905.0
86	612.5
87	320.0
88	320.0
89	320.0
90	200.5
91	81.0
92	81.0
93	81.0
94	50.0
95	19.0
96	19.0
97	19.0
98	14.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10986822164167256
2	0.04947822698208795
3	0.0367766984685998
4	0.03417865854538631
5	0.03723857223272665
6	0.0192254954317798
7	0.021015256267771315
8	0.02292048554479454
9	0.02898257869895934
10	0.0357374824993144
11	0.021650332693445722
12	0.020437914062612764
13	0.01599237908289191
14	0.021939003796024998
15	0.02124619314983474
16	0.022516346001183553
17	0.026730944098840987
18	0.020149242960033485
19	0.03233116348887895
20	0.023440093529437236
21	0.028289768052769077
22	0.03643029314550467
23	0.028462970714316645
24	0.03972114371490842
25	0.03296623991455336
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1732075.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.72840827935839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.54140934832986	67.26725998898067
2	9.65361723247709	15.007206032372583
3	2.165940098307166	5.050652288095591
4	0.7274048794777339	2.2616009382577116
5	0.31501445798339456	1.224278620201704
6	0.17419415899995308	0.8123900826376694
7	0.09570712875986619	0.5207413945644401
8	0.06228841641239627	0.387326356958194
9	0.04357408774212099	0.304825003417815
>10	0.19811242260209613	2.6467170659426635
>50	0.011609209249293918	0.6237408108078529
>100	0.00879801275137491	1.4974105263135087
>500	0.001202862920054896	0.6455357267766588
>1k	0.0010525050550480339	1.410338079637427
>5k	7.517893250343098E-5	0.3399770850354485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5818	0.3358976949612459	No Hit
CCCATGTACTCTGCGTTGATACCAC	4736	0.2734292683630905	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2481	0.1432386010998369	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2359	0.13619502619690257	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.773422051585526E-5	0.0
6	0.0	0.0	0.0	5.773422051585526E-5	0.0
7	0.0	0.0	0.0	5.773422051585526E-5	0.0
8	0.0	0.0	0.0	5.773422051585526E-5	0.0
9	0.0	0.0	0.0	5.773422051585526E-5	0.0
10	0.0	0.0	0.0	1.1546844103171051E-4	0.0
11	0.0	0.0	0.0	1.1546844103171051E-4	0.0
12	0.0	0.0	0.0	1.732026615475658E-4	0.0
13	0.0	0.0	0.0	2.3093688206342103E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCG	65	5.447669E-5	13.155266	5
CGTTTAT	95	7.408926E-8	12.997273	14
TAGGACC	390	0.0	12.668766	4
GAAGCGT	70	1.0917046E-4	12.213488	6
GTAGGAC	880	0.0	11.768315	3
TCCAACG	115	7.069502E-8	11.565798	18
AGGACGT	675	0.0	11.541986	5
GACGTGG	330	0.0	11.513401	7
GTATTAG	270	0.0	11.273827	1
TGTAGGA	975	0.0	11.109854	2
ATAGGAC	120	1.2734563E-7	11.084531	3
GCGTTTA	120	1.2790588E-7	11.081009	13
GGACGTG	690	0.0	11.013773	6
TGGCGAG	830	0.0	10.988503	18
TAGGACG	715	0.0	10.896911	4
CGTGGAA	370	0.0	10.782145	9
GGCGAGA	495	0.0	10.556086	19
CCAACGA	90	9.510758E-5	10.556085	19
GTTCTAA	235	0.0	10.524238	1
TTAGGAC	625	0.0	10.489132	3
>>END_MODULE