FastQCFastQC Report
Thu 26 May 2016
SRR1513022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732075
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC67200.38797396186654737No Hit
CCCATGTACTCTGCGTTGATACCAC66320.3828933504611521No Hit
GTATCAACGCAGAGTACTTTTTTTT37680.21754254290374261No Hit
TATCAACGCAGAGTACTTTTTTTTT27370.15801856155189586No Hit
GAGTACATGGGAAGCAGTGGTATCA25130.14508609615634427No Hit
GGTATCAACGCAGAGTACTTTTTTT21520.12424404255012053No Hit
CATGTACTCTGCGTTGATACCACTG20280.11708499920615448No Hit
GTATCAACGCAGAGTACATGGGAAG18510.10686604217484809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA854.0146024E-612.27463219
TAGGACC4350.011.82082754
AGGACGT7550.011.6033735
GGACGTG7300.011.4789776
TAGGACG8200.011.1480974
GACGTGG3500.011.1138227
GTCCTAC8700.011.063321
CTAACAC1900.011.025863
TCCAACG1553.1832315E-1011.01954618
GTAAGAC1657.6397555E-1110.9651123
GACGTGA4250.010.7151917
AGGACCG803.690182E-410.71262555
GTCCTAA4200.010.664291
TAGACAG3850.010.6353345
TGGCGAG9500.010.58778618
GTAGGAC9500.010.4244493
GGCGAGG4750.010.38351719
CCGGTTT1106.1100927E-610.35438512
TCTATAC3250.010.254823
TTAGGAC6400.010.1174793
Produced by FastQC (version 0.11.5)