##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513022_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1732075
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.608477692940546	31.0	31.0	34.0	27.0	34.0
2	30.73290994904955	31.0	31.0	34.0	27.0	34.0
3	30.751390673036674	31.0	31.0	34.0	27.0	34.0
4	33.99939205865797	37.0	35.0	37.0	30.0	37.0
5	33.97624179091552	37.0	35.0	37.0	30.0	37.0
6	33.945482730251285	37.0	35.0	37.0	30.0	37.0
7	33.85593060346694	37.0	35.0	37.0	29.0	37.0
8	33.84656611289927	37.0	35.0	37.0	29.0	37.0
9	35.395929737453635	39.0	35.0	39.0	29.0	39.0
10	35.299146976891876	39.0	35.0	39.0	29.0	39.0
11	35.32082559935338	39.0	35.0	39.0	29.0	39.0
12	35.24401310566806	39.0	35.0	39.0	29.0	39.0
13	35.22311966860558	39.0	35.0	39.0	29.0	39.0
14	36.39191663178558	40.0	36.0	41.0	29.0	41.0
15	36.38956338495735	40.0	36.0	41.0	29.0	41.0
16	36.3504305529495	40.0	36.0	41.0	28.0	41.0
17	36.26357634628985	40.0	36.0	41.0	28.0	41.0
18	36.183325779772815	40.0	36.0	41.0	27.0	41.0
19	36.26024219505506	40.0	37.0	41.0	27.0	41.0
20	36.153499126769916	40.0	36.0	41.0	27.0	41.0
21	36.17136498130855	40.0	37.0	41.0	27.0	41.0
22	36.154534301343766	40.0	37.0	41.0	27.0	41.0
23	36.09333718228137	40.0	37.0	41.0	27.0	41.0
24	35.96931599382244	40.0	36.0	41.0	27.0	41.0
25	35.95293968217312	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6065604448913113
1101	2	0.40834466485501153
1101	3	0.5819930205391444
1101	4	0.8123946210854669
1101	5	0.6187131096984899
1101	6	0.5309643500933703
1101	7	0.4909499821754011
1101	8	0.5061235603461824
1101	9	0.6249983145701492
1101	10	0.6036977989544496
1101	11	0.48170272045938844
1101	12	0.5684465791922833
1101	13	0.5215780197313578
1101	14	0.5211977906335079
1101	15	0.5753064665502308
1101	16	0.585083315898622
1101	17	0.504047598786002
1101	18	0.4065047933859276
1101	19	0.5770621828451326
1101	20	0.6523655790544183
1101	21	0.6725572381838489
1101	22	0.5498122779641292
1101	23	0.5630387559235288
1101	24	0.6985926751243099
1101	25	0.7063098531017573
1102	1	0.606610405102046
1102	2	0.5550898631420544
1102	3	0.509490815730107
1102	4	0.8715976033257391
1102	5	0.5785291978139924
1102	6	0.3771069116209631
1102	7	0.4282228835318094
1102	8	0.45176102016763764
1102	9	0.5114394181739712
1102	10	0.44521075846083846
1102	11	0.4830574770491296
1102	12	0.5330580524155337
1102	13	0.5517637935282238
1102	14	0.5380345141713221
1102	15	0.47056230059752124
1102	16	0.5225829597499043
1102	17	0.5283664270931681
1102	18	0.49292138070597247
1102	19	0.48704315953411736
1102	20	0.4896484857050254
1102	21	0.5450705747109268
1102	22	0.5669490278072544
1102	23	0.4696635196229977
1102	24	0.6091934499714
1102	25	0.6416405347008265
1103	1	0.019384182538132677
1103	2	0.09624206439062633
1103	3	0.3342293512494976
1103	4	-0.5244587701342596
1103	5	-0.13178689327241244
1103	6	-0.027513121549361585
1103	7	0.054912004792555535
1103	8	0.10704810535239062
1103	9	0.1876634390080696
1103	10	0.11878186560566917
1103	11	0.1593159102122783
1103	12	0.2221288153711498
1103	13	0.30535200399613416
1103	14	0.32873477342405266
1103	15	0.3242024459942243
1103	16	0.1746638970204515
1103	17	0.338039482279072
1103	18	0.2479173323130084
1103	19	0.36571095675346754
1103	20	0.4118499887458995
1103	21	0.35573486319119496
1103	22	0.33338648550876826
1103	23	0.324760453616328
1103	24	0.32575663133716404
1103	25	0.43819972370902605
1104	1	0.17350429537044576
1104	2	0.1651416079135366
1104	3	0.35122957712423997
1104	4	0.05326735411785677
1104	5	0.06645010185815181
1104	6	0.136642658480163
1104	7	0.03392535241377459
1104	8	0.050177967568799886
1104	9	0.004110534416504663
1104	10	-0.06108599767182454
1104	11	0.0018896215443646724
1104	12	0.09731923302930312
1104	13	0.051171351480782334
1104	14	-0.01682192901486701
1104	15	-0.07823752648057081
1104	16	0.013235291823228579
1104	17	-0.0828376471156318
1104	18	-0.19246368685482906
1104	19	-0.12220723982058956
1104	20	0.01018810221906108
1104	21	-0.010405856771406263
1104	22	0.017985636639984648
1104	23	0.030038043165156125
1104	24	-0.021821912663760656
1104	25	0.0333352748171265
1105	1	0.39160963042159125
1105	2	0.34895005624203534
1105	3	0.40908339744720834
1105	4	0.38736283761495116
1105	5	0.3793278784286258
1105	6	0.36982283740177024
1105	7	0.1628117749647302
1105	8	0.22008403113822794
1105	9	0.2271305221020654
1105	10	0.34145851282908524
1105	11	0.15722544881101896
1105	12	0.29320100920654824
1105	13	0.18874320728797755
1105	14	0.21772693810815724
1105	15	0.24321650908643733
1105	16	0.25506235669136856
1105	17	0.22376713689007488
1105	18	0.1265436293728115
1105	19	0.24915353195346768
1105	20	0.39555370663570955
1105	21	0.41177475677758935
1105	22	0.36149558908967805
1105	23	0.42250346700446784
1105	24	0.49880025126319794
1105	25	0.5699787613566158
1106	1	-0.6705753113684487
1106	2	-0.4384199345863422
1106	3	-0.2694794701084575
1106	4	-1.4848051573761154
1106	5	-0.6442225941758295
1106	6	-0.4672565444979355
1106	7	-0.3314757834617552
1106	8	-0.19811066253985388
1106	9	-0.03745312392995004
1106	10	0.022301027256986572
1106	11	-0.06903737379440855
1106	12	-0.031681785122437134
1106	13	-0.07920576237721377
1106	14	-0.12968126498903132
1106	15	0.012125030989317054
1106	16	-0.028732843209148484
1106	17	-0.14965067309441338
1106	18	-0.13966337186413824
1106	19	-0.3221742294322567
1106	20	-0.13031723512324334
1106	21	-0.1937499284238342
1106	22	-0.04159890972569258
1106	23	-0.09592005352744337
1106	24	0.013000069868574826
1106	25	-0.0250008425248609
1107	1	0.5441069380749255
1107	2	0.29486306311704524
1107	3	0.5192948179161121
1107	4	0.8747787189951595
1107	5	0.6115769329528291
1107	6	0.5698886296818557
1107	7	0.4916366205687197
1107	8	0.48561852231834024
1107	9	0.6365445058054107
1107	10	0.5266797938134857
1107	11	0.5475815764280583
1107	12	0.6066697111809702
1107	13	0.6121044882493223
1107	14	0.5585080190358696
1107	15	0.5012526530798098
1107	16	0.5423701727143211
1107	17	0.572921233401047
1107	18	0.6050593627075784
1107	19	0.5241902422779745
1107	20	0.6890546565629876
1107	21	0.5845275296032213
1107	22	0.6655796679343453
1107	23	0.41548600809549896
1107	24	0.5583113925888838
1107	25	0.5788798061036928
1108	1	-0.47922017452196286
1108	2	-0.33653156056734446
1108	3	-0.0957261056711225
1108	4	-1.2192415072257106
1108	5	-0.714757033358616
1108	6	-0.5867208768288847
1108	7	-0.6515336546962729
1108	8	-0.4301472280099645
1108	9	-0.30221719896716337
1108	10	-0.14032685842813208
1108	11	-0.30721186967819136
1108	12	-0.08553118329654552
1108	13	-0.17029669524838198
1108	14	-0.21963081762410752
1108	15	-0.056286562743920854
1108	16	-0.16821408840706198
1108	17	-0.18804748792605608
1108	18	-0.44645968089832877
1108	19	-0.20385167447735597
1108	20	-0.11634611619994928
1108	21	-0.1108866764194687
1108	22	-0.026067825051747207
1108	23	-0.1072834191138341
1108	24	-0.15925925405095853
1108	25	-0.25323638146760885
1109	1	-0.1470972318229471
1109	2	-0.2732758768924448
1109	3	0.21148358990279448
1109	4	-0.60214794898328
1109	5	-0.20950828991370685
1109	6	0.03132864881832376
1109	7	0.06866900209431037
1109	8	0.16231370077746732
1109	9	0.1386360136123912
1109	10	0.26591649570514164
1109	11	0.25774126561479704
1109	12	0.15234105007776577
1109	13	0.2527725670020544
1109	14	0.2922360419996295
1109	15	0.4379070893835362
1109	16	0.38762753118741955
1109	17	0.12038815933598102
1109	18	0.2887775473667702
1109	19	0.259875509127518
1109	20	0.41679944708426575
1109	21	0.5560932861302632
1109	22	0.43134468292328876
1109	23	0.4970007983467042
1109	24	0.42402895222637227
1109	25	0.4531809971172649
1110	1	0.43161797932166834
1110	2	0.17505867487580673
1110	3	0.24600007431190463
1110	4	0.7432702280350085
1110	5	0.4594726415797865
1110	6	0.3055417429322702
1110	7	0.2875588048093931
1110	8	0.24426385663559813
1110	9	0.18752495326940277
1110	10	0.21534890810475105
1110	11	0.2123342575832865
1110	12	0.13188835073673744
1110	13	0.16122698230559962
1110	14	0.14216522046988445
1110	15	0.17982175832461422
1110	16	0.22944726374095126
1110	17	0.2369923812129784
1110	18	0.06613445741581359
1110	19	-0.008766545153754635
1110	20	0.1911904834647018
1110	21	0.2280276531025578
1110	22	0.3960945991558873
1110	23	0.1936696480426079
1110	24	0.3988477100319727
1110	25	0.33880803418814764
1111	1	0.46770823431985065
1111	2	-0.021706972462460783
1111	3	0.36720021707346717
1111	4	0.7121351922734434
1111	5	0.5985149290999487
1111	6	0.5614634498073983
1111	7	0.34567821611114624
1111	8	0.33498684633114095
1111	9	0.4373538732639517
1111	10	0.434376563581651
1111	11	0.3486888350029602
1111	12	0.21609504792156997
1111	13	0.3435636962272923
1111	14	0.45691658758469345
1111	15	0.4585072741270224
1111	16	0.5276216646813054
1111	17	0.4684186342914032
1111	18	0.5475607104942384
1111	19	0.40316106131241014
1111	20	0.6315758379994136
1111	21	0.6095731791951806
1111	22	0.5786588266063788
1111	23	0.5143821238168655
1111	24	0.5175896898623265
1111	25	0.532421284576067
1112	1	0.04349300231567099
1112	2	-0.4376267589852638
1112	3	-0.03519052451325422
1112	4	0.24820116426906225
1112	5	0.043805958561833336
1112	6	0.05073701822669818
1112	7	-0.14241883479997597
1112	8	-0.16477748760404154
1112	9	-0.21128252662872882
1112	10	-0.18015714391688675
1112	11	-0.15578338178081452
1112	12	-0.09863879445046564
1112	13	-0.08570204082739252
1112	14	-0.15343495367137194
1112	15	-0.12765405198405233
1112	16	-0.22218255120331065
1112	17	-0.2323325966105827
1112	18	-0.23183409905132635
1112	19	-0.21491851405360052
1112	20	-0.14717904753938882
1112	21	-0.14683693776770212
1112	22	-0.12425509364422993
1112	23	-0.3251818577069159
1112	24	-0.3766669468566235
1112	25	-0.33508181533909465
1113	1	0.24869865695254134
1113	2	0.1797073920317871
1113	3	0.1489160431695673
1113	4	0.5115531596686935
1113	5	0.33221657429203333
1113	6	0.2948393333386079
1113	7	0.1207311763059522
1113	8	0.04415250092068135
1113	9	-0.01593588567241966
1113	10	-0.029282955893741303
1113	11	-0.04076018757169919
1113	12	0.013589834841795323
1113	13	-0.009999738232089328
1113	14	-0.06293637179347655
1113	15	-0.07489229188137614
1113	16	0.030220484793751723
1113	17	0.06412289323274933
1113	18	0.0056894273097398695
1113	19	-0.23234379525420934
1113	20	0.09379539651006041
1113	21	0.04410567612194427
1113	22	-0.0595543304898527
1113	23	-0.22818233118375275
1113	24	-0.06496722145266887
1113	25	-0.06162162817467731
1114	1	0.13998650880918007
1114	2	-0.1253814487399545
1114	3	0.00976185643912686
1114	4	0.5116441657120632
1114	5	0.15141057616644815
1114	6	0.09698521447489128
1114	7	-0.1395212879024328
1114	8	-0.14871399554055387
1114	9	-0.16598959733579477
1114	10	-0.05215714391688664
1114	11	-0.041968085965521595
1114	12	-0.3246820844937517
1114	13	-0.17506712019247317
1114	14	-0.1448548670912828
1114	15	-0.326485220815222
1114	16	-0.3646500836708455
1114	17	-0.29665582893381526
1114	18	-0.27896829818552504
1114	19	-0.39905704219212623
1114	20	-0.2600477344080758
1114	21	-0.24974314267390696
1114	22	-0.26459275598188725
1114	23	-0.24363784616290474
1114	24	-0.2095702657599432
1114	25	-0.25677012702740143
1115	1	0.11923460842050204
1115	2	0.01721147252720101
1115	3	0.09480736257884459
1115	4	0.5565870336651457
1115	5	0.16766208099910784
1115	6	0.1260568617077169
1115	7	-0.02602381602130066
1115	8	-0.15873195034397725
1115	9	-0.30522421364646135
1115	10	-0.1946895279157843
1115	11	-0.14125140726019225
1115	12	-0.14486479697612964
1115	13	-0.3206496194185533
1115	14	-0.21290962580211215
1115	15	-0.16078371082313225
1115	16	-0.3788804692519676
1115	17	-0.33694283061922903
1115	18	-0.16472470447314436
1115	19	-0.2650533961975654
1115	20	-0.028910665650123235
1115	21	-0.29745737925080107
1115	22	-0.18657727770307275
1115	23	-0.14654982635070724
1115	24	-0.040211341672296896
1115	25	-0.13826567272272428
1116	1	-0.049279072770811894
1116	2	-0.27726235386103326
1116	3	-0.0582476711511859
1116	4	0.27912014824481446
1116	5	0.045551108123575546
1116	6	-0.10952530557274542
1116	7	-0.2634450671799229
1116	8	-0.1702527013761994
1116	9	-0.289095591778036
1116	10	-0.31870418639405784
1116	11	-0.40492640275789427
1116	12	-0.4545018542436523
1116	13	-0.31578168946599305
1116	14	-0.3505587178256988
1116	15	-0.36208664995377404
1116	16	-0.3346619931587327
1116	17	-0.29200795279248837
1116	18	-0.4447436058473002
1116	19	-0.4998652994370687
1116	20	-0.5972275676752474
1116	21	-0.4904688274694635
1116	22	-0.25548914343723084
1116	23	-0.39075178026374147
1116	24	-0.48709468021012015
1116	25	-0.47064865025090796
1201	1	0.5588088383121388
1201	2	0.5835895174666135
1201	3	0.740267873981793
1201	4	0.8799732607008437
1201	5	0.6911753140463688
1201	6	0.5753492588075204
1201	7	0.6149227183530925
1201	8	0.5912419438080576
1201	9	0.6452219345877737
1201	10	0.5679220020680233
1201	11	0.609281251084866
1201	12	0.4922589639865578
1201	13	0.6515678155524114
1201	14	0.7055494301821241
1201	15	0.6029674207840117
1201	16	0.7491406282350539
1201	17	0.653587184597157
1201	18	0.785085281742397
1201	19	0.7380403944026881
1201	20	0.8456116114816368
1201	21	0.6910426056092263
1201	22	0.702511072127173
1201	23	0.6055672942464199
1201	24	0.7498128851205905
1201	25	0.6858435943297749
1202	1	0.5639941384968381
1202	2	0.5145086020110945
1202	3	0.541102297189834
1202	4	0.86663554825234
1202	5	0.6958378386753878
1202	6	0.5220201271081564
1202	7	0.33295039028824647
1202	8	0.3451680849117764
1202	9	0.4186643758921704
1202	10	0.5103806809409122
1202	11	0.437581217484869
1202	12	0.5742083409294523
1202	13	0.4692613651101638
1202	14	0.6604263754578525
1202	15	0.6299683368672575
1202	16	0.5687686295005889
1202	17	0.5054725814343826
1202	18	0.7138765534783573
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2210	14	0.008512599435711365
2210	15	-0.08495053655817486
2210	16	0.06059363911699478
2210	17	-0.05777462947069267
2210	18	-0.03984969406728567
2210	19	0.08563317288627559
2210	20	0.16317785239259308
2210	21	0.0043229194598524145
2210	22	-0.05196251405960339
2210	23	-0.09756916511403091
2210	24	-0.03783791888463384
2210	25	-0.024049401587916464
2211	1	0.03352207980438493
2211	2	-0.038470270216286906
2211	3	0.14505728652034833
2211	4	0.36598790449929197
2211	5	0.07300499179746112
2211	6	0.0036884143966062766
2211	7	-0.16031888714567089
2211	8	-0.22733447373476423
2211	9	-0.3451562476929766
2211	10	-0.3150855452607857
2211	11	-0.21309298403121346
2211	12	-0.2702588858767996
2211	13	-0.2544186549153693
2211	14	-0.2534761933789156
2211	15	-0.2691097437626766
2211	16	-0.3202060741460997
2211	17	-0.25556818174067786
2211	18	-0.2686399555557841
2211	19	-0.29819942402988175
2211	20	-0.1329762009899369
2211	21	-0.18906414603365818
2211	22	-0.274858078746675
2211	23	-0.24687495351196276
2211	24	-3.309646985595542
2211	25	-2.389545023462148
2212	1	0.18540598547494724
2212	2	-0.006596447391153504
2212	3	-0.3668089555382146
2212	4	0.30787858707704885
2212	5	0.20669652167494945
2212	6	0.14778100622023516
2212	7	0.10244876874715203
2212	8	-0.0488941951628874
2212	9	-0.15870931678549027
2212	10	-0.18076410372265883
2212	11	-0.18182538297118356
2212	12	-0.18777838878293807
2212	13	-0.13161689089654516
2212	14	-0.09878769687277611
2212	15	-0.19313345388778913
2212	16	-0.2673962395920739
2212	17	-0.258356934949461
2212	18	-0.4217964611205147
2212	19	-0.32411989625201443
2212	20	-0.5162031161183549
2212	21	-0.5109014917432333
2212	22	-0.4003302795858801
2212	23	-0.24447814829400727
2212	24	-0.25474884682279253
2212	25	-0.3170560725359053
2213	1	0.11214741922336557
2213	2	-0.3356320772962569
2213	3	-0.12651550230111397
2213	4	0.5091055922040582
2213	5	0.24031093345108445
2213	6	0.16725780941997215
2213	7	0.13939336076767717
2213	8	-0.022290547082171486
2213	9	-0.08975061361209669
2213	10	-0.2655746376313175
2213	11	-0.1400064609820575
2213	12	-0.19081917593215536
2213	13	-0.18412230081970193
2213	14	-0.07006381243996884
2213	15	-0.16521619871650728
2213	16	-0.21544801935960578
2213	17	-0.2297080983529014
2213	18	-0.14838261603652825
2213	19	-0.19280561966996146
2213	20	-5.845262502173064
2213	21	-2.61223010840105
2213	22	-1.4178617780806064
2213	23	-0.9031244149071185
2213	24	-0.493208217328025
2213	25	-0.2813473402072404
2214	1	0.05366077894284871
2214	2	-0.2662555592673179
2214	3	0.12134874212111058
2214	4	0.43965879628996873
2214	5	0.1811784819987281
2214	6	0.031101062050872486
2214	7	0.018364460584635367
2214	8	-0.1054465884779745
2214	9	-0.1718279959525404
2214	10	-0.283967744435607
2214	11	-0.4210008717605547
2214	12	-0.3430280311586458
2214	13	-0.25482904078833
2214	14	-0.25267983217740664
2214	15	-0.15152611993073606
2214	16	-0.25014453070149756
2214	17	-0.14274986364818432
2214	18	-0.14700208151997174
2214	19	-0.1850002489487963
2214	20	-0.08863641566098579
2214	21	-0.26068442085439614
2214	22	-0.07200540478151396
2214	23	-0.2586900507249865
2214	24	-0.3136290540820781
2214	25	-0.24846926914351997
2215	1	-0.4292263515012422
2215	2	-0.30770988554704815
2215	3	-0.36671735642118364
2215	4	-0.029319248922085706
2215	5	-0.39973825975372534
2215	6	-0.38095963031446445
2215	7	-0.49705953623945476
2215	8	-0.6354010797977594
2215	9	-0.8268898190652862
2215	10	-0.9312311461341807
2215	11	-0.8212347156772708
2215	12	-0.7728977164228041
2215	13	-0.9657184505694119
2215	14	-1.015233470195497
2215	15	-1.0160667064028104
2215	16	-1.1083779107218419
2215	17	-0.9284169154083202
2215	18	-0.8706163026201139
2215	19	-0.9283743925247236
2215	20	-1.0640574350732663
2215	21	-0.949511989680559
2215	22	-1.0955935874674765
2215	23	-1.113665562349162
2215	24	-1.096207738043752
2215	25	-1.1336395837901563
2216	1	-1.2916367477863524
2216	2	-0.8560825073855582
2216	3	-2.8306370681113684
2216	4	-0.9985074054524503
2216	5	-0.8433365251610567
2216	6	-0.8152312197378464
2216	7	-0.7014278582267437
2216	8	-0.7671939148818367
2216	9	-0.8083717179877894
2216	10	-0.8913821481178559
2216	11	-0.816908096047861
2216	12	-0.8909506107918546
2216	13	-0.8986833191506278
2216	14	-0.9969506493127582
2216	15	-0.970657628813882
2216	16	-0.9976764657914927
2216	17	-1.0739651887052801
2216	18	-1.1487048130569804
2216	19	-1.025572585798244
2216	20	-0.8995409288291825
2216	21	-1.0332252465220009
2216	22	-1.1142687768187258
2216	23	-1.1192587502126443
2216	24	-1.0765146448558909
2216	25	-1.0098603639623747
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40134.0
3	2.0
4	664.0
5	4960.0
6	5806.0
7	4481.0
8	3778.0
9	3240.0
10	2888.0
11	2634.0
12	2477.0
13	2370.0
14	2370.0
15	2343.0
16	2408.0
17	2476.0
18	2902.0
19	3434.0
20	4474.0
21	6132.0
22	7627.0
23	9393.0
24	10837.0
25	12393.0
26	13662.0
27	15315.0
28	17208.0
29	20760.0
30	26213.0
31	33131.0
32	42889.0
33	55352.0
34	73820.0
35	99410.0
36	143820.0
37	236600.0
38	813672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69058447982025	19.515287970593782	13.35162158816254	27.44250596142343
2	13.484185952196498	20.815373656598847	39.817939283438506	25.88250110776615
3	16.824848808510023	25.165018835789443	29.881269575509144	28.128862780191387
4	10.163878584934254	17.456461180953482	38.48124359510991	33.898416639002356
5	12.38982256119814	38.42334237234893	36.22620329007849	12.960631776374445
6	31.79071414578279	37.71884168514618	17.888594324345313	12.601849844725718
7	27.422974443652194	31.69703040607833	22.390917147550166	18.489078002719314
8	24.991524821732494	37.23753657898491	20.37906425946578	17.39187433981682
9	26.105892123467655	14.90651447474961	19.716684627992638	39.27090877379009
10	13.637736175863512	29.841428385848936	35.264675740823606	21.25615969746395
11	34.31581305441302	22.748572180793207	23.973967847797823	18.961646916995957
12	22.415418770551675	25.45009425982222	32.096205645196214	20.038281324429892
13	28.73798886100741	21.65682987919863	25.754561086046003	23.85062017374796
14	20.582573823438054	22.00159580794417	28.098171056293218	29.317659312324558
15	23.557351731304937	30.082819739329995	23.428777622216128	22.93105090714894
16	21.945201809361144	28.55263811275826	27.632714007074384	21.86944607080621
17	21.874747348749967	28.511130514727114	27.66233781233469	21.951784324188225
18	21.670898508023168	27.984632494186634	29.046434709765013	21.29803428802519
19	22.811402553856976	27.59143832471787	28.04576406362524	21.551395057799912
20	22.994154410172772	27.564279837766843	28.223373699175845	21.218192052884547
21	22.79300838589553	26.969906037556115	27.805204739979505	22.431880836568855
22	22.52656495821486	27.796660075343162	27.269835313135975	22.406939653306004
23	21.909802929897914	27.5823088389988	27.59922497537634	22.90866325572695
24	22.16986360844122	27.8437616911999	28.058706566470676	21.927668133888197
25	22.44217048463287	27.011432536947034	27.64691346905502	22.899483509365073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	152.0
2	309.5
3	467.0
4	467.0
5	467.0
6	1043.0
7	1619.0
8	1619.0
9	1619.0
10	2361.0
11	3103.0
12	3103.0
13	3103.0
14	5471.0
15	7839.0
16	7839.0
17	7839.0
18	14270.0
19	20701.0
20	20701.0
21	20701.0
22	32891.5
23	45082.0
24	45082.0
25	45082.0
26	63577.0
27	82072.0
28	82072.0
29	82072.0
30	104447.5
31	126823.0
32	126823.0
33	126823.0
34	144615.5
35	162408.0
36	162408.0
37	162408.0
38	177234.0
39	192060.0
40	192060.0
41	192060.0
42	203414.0
43	214768.0
44	214768.0
45	214768.0
46	229915.0
47	245062.0
48	245062.0
49	245062.0
50	233266.0
51	221470.0
52	221470.0
53	221470.0
54	192747.5
55	164025.0
56	164025.0
57	164025.0
58	138091.5
59	112158.0
60	112158.0
61	112158.0
62	90296.0
63	68434.0
64	68434.0
65	68434.0
66	52328.0
67	36222.0
68	36222.0
69	36222.0
70	26524.0
71	16826.0
72	16826.0
73	16826.0
74	11834.5
75	6843.0
76	6843.0
77	6843.0
78	4736.0
79	2629.0
80	2629.0
81	2629.0
82	1775.0
83	921.0
84	921.0
85	921.0
86	615.5
87	310.0
88	310.0
89	310.0
90	189.5
91	69.0
92	69.0
93	69.0
94	40.5
95	12.0
96	12.0
97	12.0
98	6.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19981813720537506
2	0.06431592165466275
3	0.0
4	0.0
5	0.01864815322662125
6	0.3891863804973803
7	0.0011546844103171052
8	0.031638352842688684
9	0.033312645237648486
10	0.002886711025792763
11	0.0036372558924988815
12	0.009237475282536841
13	0.008140525092735591
14	0.0021361661590866446
15	0.0
16	0.011142704559560065
17	0.025345322806460457
18	0.06639435359323355
19	0.028751641816895917
20	0.0
21	0.0
22	0.0
23	5.773422051585526E-5
24	1.732026615475658E-4
25	5.773422051585526E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1732075.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.61840666719364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.76691051189725	60.10401166140178
2	11.93676179547946	17.336572447070957
3	3.0292568273588403	6.599394125655509
4	1.0449190639683574	3.0352143008623
5	0.43859408717522874	1.5925001892158668
6	0.2316915750047121	1.0095043809032866
7	0.13068846704775394	0.6643271772750152
8	0.09023724289987384	0.5242307841143495
9	0.05950508208252278	0.38890478244900395
>10	0.24276576143297127	3.057919973917335
>50	0.01551810361213782	0.7960804133967596
>100	0.009883958682856462	1.5490733202536444
>500	0.0016736095249037801	0.7794435500830166
>1k	0.0014345224499175253	1.790092697328317
>5k	1.5939138332416946E-4	0.7727301960727971
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6720	0.38797396186654737	No Hit
CCCATGTACTCTGCGTTGATACCAC	6632	0.3828933504611521	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3768	0.21754254290374261	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2737	0.15801856155189586	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2513	0.14508609615634427	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2152	0.12424404255012053	No Hit
CATGTACTCTGCGTTGATACCACTG	2028	0.11708499920615448	No Hit
GTATCAACGCAGAGTACATGGGAAG	1851	0.10686604217484809	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.1546844103171051E-4	0.0
9	0.0	0.0	0.0	1.1546844103171051E-4	0.0
10	0.0	0.0	0.0	1.1546844103171051E-4	0.0
11	0.0	0.0	0.0	1.1546844103171051E-4	0.0
12	0.0	0.0	0.0	1.732026615475658E-4	0.0
13	0.0	0.0	0.0	1.732026615475658E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	85	4.0146024E-6	12.274632	19
TAGGACC	435	0.0	11.8208275	4
AGGACGT	755	0.0	11.603373	5
GGACGTG	730	0.0	11.478977	6
TAGGACG	820	0.0	11.148097	4
GACGTGG	350	0.0	11.113822	7
GTCCTAC	870	0.0	11.06332	1
CTAACAC	190	0.0	11.02586	3
TCCAACG	155	3.1832315E-10	11.019546	18
GTAAGAC	165	7.6397555E-11	10.965112	3
GACGTGA	425	0.0	10.715191	7
AGGACCG	80	3.690182E-4	10.7126255	5
GTCCTAA	420	0.0	10.66429	1
TAGACAG	385	0.0	10.635334	5
TGGCGAG	950	0.0	10.587786	18
GTAGGAC	950	0.0	10.424449	3
GGCGAGG	475	0.0	10.383517	19
CCGGTTT	110	6.1100927E-6	10.354385	12
TCTATAC	325	0.0	10.25482	3
TTAGGAC	640	0.0	10.117479	3
>>END_MODULE