FastQCFastQC Report
Thu 26 May 2016
SRR1513021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347098
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC115550.49231007823277934No Hit
CCCATGTACTCTGCGTTGATACCAC97010.41331891552887867No Hit
GAGTACATGGGAAGCAGTGGTATCA48160.2051895574875868No Hit
GCGTTGATACCACTGCTTCCCATGT33780.14392240971616865No Hit
GTATCAACGCAGAGTACATGGGAAG32770.13961922339842647No Hit
CATGTACTCTGCGTTGATACCACTG30900.13165193783983456No Hit
GTATCAACGCAGAGTACTTTTTTTT27990.11925364854812198No Hit
TATCAACGCAGAGTACATGGGAAGC25360.10804832179994188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAC1551.8189894E-1212.2583393
ATACGAC550.003070201712.09040719
TGGCGAG9550.011.33984318
GGCGAGG4850.011.16448519
TGTAGGA13750.010.917542
TAGGACC5050.010.9120664
TAGACTG3400.010.8982335
GTAGGAC12750.010.8041653
CTGTAGG14250.010.6794261
TAGGACG11250.010.4722394
TTAGGAC7600.010.3752333
CGTGCGC1106.0559178E-610.36254310
GGACGTG10750.010.3391066
TCCTACA13800.010.32722
GCGTTTA1752.3646862E-1010.31275813
TTAGACT2400.010.2927754
CCTACAC3600.010.2918983
GTCCTAC12450.010.2371311
TATGGCG10900.010.02209416
AGGACGT11100.010.0145925
Produced by FastQC (version 0.11.5)