FastQCFastQC Report
Thu 26 May 2016
SRR1513021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347098
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC136830.582975231541248No Hit
CCCATGTACTCTGCGTTGATACCAC129820.5531085621478098No Hit
GAGTACATGGGAAGCAGTGGTATCA51080.21763045258442554No Hit
GTATCAACGCAGAGTACTTTTTTTT44340.18891413992939365No Hit
CATGTACTCTGCGTTGATACCACTG37580.16011261566410948No Hit
GTATCAACGCAGAGTACATGGGAAG35170.14984461662870488No Hit
GCGTTGATACCACTGCTTCCCATGT33570.1430276878085193No Hit
TATCAACGCAGAGTACATGGGAAGC31620.1347195558089181No Hit
ACGCAGAGTACATGGGAAGCAGTGG31310.13339877585000712No Hit
TATCAACGCAGAGTACTTTTTTTTT29660.12636881800419072No Hit
GGTATCAACGCAGAGTACTTTTTTT26330.11218108489717941No Hit
ACTCTGCGTTGATACCACTGCTTCC25050.10672754184103093No Hit
GCTTCCCATGTACTCTGCGTTGATA24000.10225393230278412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1102.8067006E-912.93655919
AGGACCG1551.8189894E-1212.2842375
TATATCG550.003020604812.1167245
TAGGACC5000.011.9955574
GGCGAGG4800.011.46322819
GCACCGT1201.2391138E-711.10699756
TGGTCCG600.005784597711.10699755
TGGCGAG11000.011.04319818
CTCGAAC1658.185452E-1110.92816418
CCGCTAG803.8209552E-410.6726619
TGAACCG909.315254E-510.5780935
TCGAACT1803.6379788E-1110.54089919
TCCAACG1553.7271093E-910.40865918
TGTAGGA11550.010.3922092
GCGTGCG1201.5518854E-610.2773779
GTCCTAA6500.010.2622681
TTAGGAC7700.010.2621243
GGACCGC1303.7325844E-710.2526126
GTCCTAC11500.010.109291
ATGGCGA12450.09.98742517
Produced by FastQC (version 0.11.5)