##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513021_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2347098
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.52162074186932	31.0	31.0	34.0	27.0	34.0
2	30.66710337616921	31.0	31.0	34.0	27.0	34.0
3	30.685980730246456	33.0	31.0	34.0	27.0	34.0
4	33.92613729805913	37.0	35.0	37.0	30.0	37.0
5	33.85011448179837	37.0	35.0	37.0	30.0	37.0
6	33.79841872814855	37.0	35.0	37.0	30.0	37.0
7	33.724832963941004	37.0	35.0	37.0	29.0	37.0
8	33.72107342769667	37.0	35.0	37.0	28.0	37.0
9	35.28233205430706	39.0	35.0	39.0	29.0	39.0
10	35.1930200613694	39.0	35.0	39.0	28.0	39.0
11	35.20116330890316	39.0	35.0	39.0	28.0	39.0
12	35.11734150001406	39.0	35.0	39.0	28.0	39.0
13	35.084294733326004	39.0	35.0	39.0	27.0	39.0
14	36.27105728009653	40.0	37.0	41.0	27.0	41.0
15	36.25611840664514	40.0	37.0	41.0	27.0	41.0
16	36.214897716243634	40.0	37.0	41.0	27.0	41.0
17	36.13287302021475	40.0	36.0	41.0	27.0	41.0
18	36.05640412117432	40.0	36.0	41.0	27.0	41.0
19	36.11896946782793	40.0	37.0	41.0	27.0	41.0
20	36.02221253650252	40.0	37.0	41.0	27.0	41.0
21	36.04213373280536	40.0	37.0	41.0	27.0	41.0
22	36.01517235326347	40.0	37.0	41.0	27.0	41.0
23	35.94846231388719	40.0	37.0	41.0	27.0	41.0
24	35.82338828630078	40.0	36.0	41.0	27.0	41.0
25	35.80413727931258	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5512913709510556
1101	2	0.4344114963911494
1101	3	0.5240211114249504
1101	4	0.7485896384551154
1101	5	0.5281120740747838
1101	6	0.41515504508781476
1101	7	0.40061438763972035
1101	8	0.3942277570271244
1101	9	0.40478555602214783
1101	10	0.4268100028115285
1101	11	0.4359318181337599
1101	12	0.33548488534185594
1101	13	0.42122540877883097
1101	14	0.35592982463308687
1101	15	0.3885585914334939
1101	16	0.4507247722707959
1101	17	0.5112474004283172
1101	18	0.25499036868765046
1101	19	0.2584114851889012
1101	20	0.5023834707331645
1101	21	0.5653756835535333
1101	22	0.4981150985652434
1101	23	0.46514021704681596
1101	24	0.5550312230635797
1101	25	0.5684694096453029
1102	1	0.4968693512722169
1102	2	0.4436384796806472
1102	3	0.525808478463901
1102	4	0.8177331699328008
1102	5	0.5147514873344434
1102	6	0.4837882800135205
1102	7	0.4195552907075353
1102	8	0.5614187899849483
1102	9	0.39938768984308837
1102	10	0.5114764573580857
1102	11	0.5600220040879194
1102	12	0.5635963853398422
1102	13	0.5512700748306116
1102	14	0.5803103197858732
1102	15	0.44394199853898186
1102	16	0.5562702901593681
1102	17	0.6087264468544262
1102	18	0.5062096431563106
1102	19	0.5299847048902961
1102	20	0.7259876062698609
1102	21	0.6822189335021847
1102	22	0.8565464300485672
1102	23	0.7007038993982846
1102	24	0.749135384779656
1102	25	0.6868330774974254
1103	1	-0.009337820838460686
1103	2	0.08085154530845884
1103	3	0.3784445787535837
1103	4	-0.5651057073022443
1103	5	-0.15750303269352628
1103	6	-0.019887676434386492
1103	7	0.044841475903822925
1103	8	0.06488098157419842
1103	9	0.1689951216680825
1103	10	0.19753579208988015
1103	11	0.3145859837103302
1103	12	0.3498634915230383
1103	13	0.23371538498943778
1103	14	0.400979583595678
1103	15	0.22268737862088983
1103	16	0.2475518804100929
1103	17	0.2767895774108169
1103	18	0.2989416379620238
1103	19	0.27513503952220475
1103	20	0.3589992934140014
1103	21	0.30706560216665224
1103	22	0.31709582571333783
1103	23	0.19392935140108136
1103	24	0.23302910169105218
1103	25	0.31485425420304836
1104	1	0.4394780331555346
1104	2	0.4399801497120812
1104	3	0.5604421150218677
1104	4	0.37256235866836107
1104	5	0.42200924410553853
1104	6	0.4724297861611433
1104	7	0.45853145234288917
1104	8	0.39771295746312063
1104	9	0.5037392404056007
1104	10	0.32629519553192665
1104	11	0.45733842651228684
1104	12	0.4824751667410041
1104	13	0.44943805969313644
1104	14	0.45467936712216783
1104	15	0.38610478978900886
1104	16	0.5654536420978644
1104	17	0.47183628067466543
1104	18	0.47343794834438313
1104	19	0.6383799894701809
1104	20	0.701480247332654
1104	21	0.6299572577704424
1104	22	0.6524487014623261
1104	23	0.6230427667790721
1104	24	0.6519706538420351
1104	25	0.6502709956188539
1105	1	0.38990791692798865
1105	2	0.26812825958570485
1105	3	0.27514897828735485
1105	4	0.34848839767810347
1105	5	0.3482098419187736
1105	6	0.2873906182770867
1105	7	0.20294969613733116
1105	8	0.17999072297565277
1105	9	0.22763673845229704
1105	10	0.1999514858017264
1105	11	0.20438388568389598
1105	12	0.2117449984670685
1105	13	0.17227521430254455
1105	14	0.21430066856377294
1105	15	0.14034201423202575
1105	16	0.17754378068468668
1105	17	0.1323264311613812
1105	18	0.025984447974074953
1105	19	-0.011421391857318497
1105	20	0.12125615348630703
1105	21	0.20847653482039874
1105	22	0.11869166917132645
1105	23	0.09218257647466999
1105	24	0.2609569548687176
1105	25	0.24437083888469147
1106	1	-0.41665137773327743
1106	2	-0.2651603397823017
1106	3	-0.09718073044103903
1106	4	-1.4978111777687744
1106	5	-0.5241219907552193
1106	6	-0.3234087797809977
1106	7	-0.27220179231587593
1106	8	-0.19125742545510604
1106	9	-0.0738029820049988
1106	10	-0.09439496433786587
1106	11	-0.10980525088501736
1106	12	-0.049695634500181995
1106	13	-0.1256621198625396
1106	14	-0.08592258171803735
1106	15	-0.01849576273637865
1106	16	-0.022346954113956485
1106	17	-0.17556835329353504
1106	18	-0.13000134204322222
1106	19	-0.2836284623935512
1106	20	-0.012512300257697007
1106	21	-0.02190690029188147
1106	22	-0.005335190087386366
1106	23	-0.04852425688763162
1106	24	0.06127916528249955
1106	25	0.05158847801688893
1107	1	0.6485512071131438
1107	2	0.31768555077825766
1107	3	0.5557523245469369
1107	4	0.8824810910104617
1107	5	0.598521164219278
1107	6	0.44816567729481704
1107	7	0.3778415222580591
1107	8	0.28538485215316456
1107	9	0.42684219903330956
1107	10	0.3890383647501494
1107	11	0.39476885118356364
1107	12	0.4124943263628822
1107	13	0.5084856997347202
1107	14	0.5662382402498309
1107	15	0.5765078950071185
1107	16	0.5580497249379732
1107	17	0.4792760187958791
1107	18	0.5053447807794385
1107	19	0.4683626851903995
1107	20	0.5384856884448297
1107	21	0.6117689350364444
1107	22	0.5840816413161747
1107	23	0.5228432715790845
1107	24	0.5446528906865566
1107	25	0.5301499942484753
1108	1	-0.494007056887245
1108	2	-0.30154548875638554
1108	3	-0.0926282849342428
1108	4	-1.017105073755964
1108	5	-0.7299831903761529
1108	6	-0.5455004271397925
1108	7	-0.48607804612070993
1108	8	-0.5027469155801185
1108	9	-0.22482733285443857
1108	10	-0.07924485424464223
1108	11	-0.2249521141256352
1108	12	-0.15964099316332891
1108	13	-0.15487515053315093
1108	14	-0.20141733314577692
1108	15	-0.03129334514706272
1108	16	-0.0670779936432595
1108	17	-0.10240710937681285
1108	18	-0.3331317595472001
1108	19	-0.19797150770925498
1108	20	-0.1134521200394687
1108	21	-0.1251260573139561
1108	22	-0.122804214763228
1108	23	-0.1353007138893787
1108	24	-0.14894955718533964
1108	25	-0.11778393807824727
1109	1	-0.19786205106482058
1109	2	-0.38064647108317473
1109	3	0.053777087277580904
1109	4	-0.639429857371077
1109	5	-0.35613268749339255
1109	6	-0.11547215840184322
1109	7	-0.12281019898189527
1109	8	0.09654761919828303
1109	9	0.12154023931958591
1109	10	0.1513936344583584
1109	11	0.0913712021976778
1109	12	0.06796062153505744
1109	13	0.06993752589243485
1109	14	0.2363836530423029
1109	15	0.20023188637816958
1109	16	0.1439444431856316
1109	17	0.04838523496392355
1109	18	0.02327246782477488
1109	19	0.010115668119851762
1109	20	0.21887998648618634
1109	21	0.14522685820024606
1109	22	0.08083976307429452
1109	23	0.14948328057203497
1109	24	0.262496402647713
1109	25	0.1673841535414553
1110	1	0.33232739999372995
1110	2	0.055330131738919874
1110	3	0.1868973075759115
1110	4	0.6112766626487485
1110	5	0.3727890258703752
1110	6	0.24969470569205754
1110	7	0.16353794197998184
1110	8	0.16446222225237506
1110	9	0.05732563942901692
1110	10	0.10570468077584394
1110	11	0.04439787303550702
1110	12	0.144805658144044
1110	13	-0.004881318507955257
1110	14	0.1565878994268104
1110	15	0.08377259153904504
1110	16	0.08799095751111707
1110	17	0.07956108990587296
1110	18	0.08416361160217889
1110	19	-0.06486054687782428
1110	20	0.06882898933218229
1110	21	0.17918199945152935
1110	22	0.26192498895566274
1110	23	0.20919842880649497
1110	24	0.24166636465029967
1110	25	0.3064802092147687
1111	1	0.2397331635088129
1111	2	-0.29891136776533855
1111	3	0.04405971784798268
1111	4	0.4433226138706061
1111	5	0.26147707400125597
1111	6	0.16584447543277747
1111	7	0.05835985269175126
1111	8	-0.04944140448857581
1111	9	0.04178749769634038
1111	10	6.647499165239878E-4
1111	11	0.09477159408727687
1111	12	0.0874857058083478
1111	13	0.07193298112529334
1111	14	0.11441734402563242
1111	15	0.08801633531369646
1111	16	0.2238660914526065
1111	17	0.14918714871547678
1111	18	0.15268769076517685
1111	19	0.021794449582806408
1111	20	0.10641565819452126
1111	21	0.0035868532508871454
1111	22	0.10136781237932269
1111	23	0.10972278757412823
1111	24	0.04261530506877875
1111	25	0.05354500373108806
1112	1	0.26947838839457816
1112	2	-0.2548323452690511
1112	3	0.10284377574947356
1112	4	0.5296877225271786
1112	5	0.3165633997008328
1112	6	0.17758406588114184
1112	7	0.08319946055936356
1112	8	0.012941756782012703
1112	9	-0.049460940975279755
1112	10	0.06202794472092421
1112	11	-0.0054056548022245465
1112	12	0.030938130980970868
1112	13	-0.018192051084731986
1112	14	-0.009934794019635262
1112	15	0.0185067102291967
1112	16	-0.010854540609635421
1112	17	-0.07063911454370242
1112	18	-0.11918749805526829
1112	19	-0.09160281278078486
1112	20	-0.05184668572747597
1112	21	-0.09382199609024156
1112	22	-0.061364455338100754
1112	23	-0.08676141292173156
1112	24	-0.025752305675339926
1112	25	-0.13960690066149084
1113	1	0.3708447524566054
1113	2	0.21798986276220234
1113	3	0.27493085405322404
1113	4	0.7093585361188701
1113	5	0.3700908228479207
1113	6	0.39088067926837056
1113	7	0.2555179295434442
1113	8	0.19224530574530263
1113	9	0.17198671285309075
1113	10	0.19546419128200654
1113	11	0.04549114816616395
1113	12	0.16863806851805663
1113	13	0.2149062701249136
1113	14	0.13981212155097467
1113	15	0.2588397256854549
1113	16	0.13501123966920403
1113	17	0.16736540127514132
1113	18	0.09218691091749776
1113	19	0.009987889922129511
1113	20	0.18641728928512435
1113	21	0.1394697325377834
1113	22	0.012197602399531604
1113	23	-0.01562309226508063
1113	24	-0.03078061926458986
1113	25	0.0030888133323614397
1114	1	0.15979083880023026
1114	2	-0.06943223438381452
1114	3	0.08393722507031853
1114	4	0.5287263736570864
1114	5	0.2194822782897461
1114	6	0.13888985973766665
1114	7	-0.08499475211082341
1114	8	-0.09345138839451295
1114	9	-0.22418645880038923
1114	10	-0.17269569066589696
1114	11	-0.07618993767911775
1114	12	-0.12997503021689027
1114	13	-0.12309174941368894
1114	14	-0.008674247384291789
1114	15	-0.16660808072053612
1114	16	-0.2217560567968846
1114	17	-0.14998608708140182
1114	18	-0.11220990388010676
1114	19	-0.16234443163649814
1114	20	-0.020199737716438904
1114	21	0.026031407310100008
1114	22	-0.030051605773088852
1114	23	-0.007551844639031913
1114	24	-0.03418659105985711
1114	25	-0.11781389879760695
1115	1	0.30946858302637636
1115	2	0.14322874856669188
1115	3	0.2068071980541717
1115	4	0.652406665451366
1115	5	0.4309870827249682
1115	6	0.39080492534002786
1115	7	0.20016988737847186
1115	8	0.14800009600287467
1115	9	-0.1275521308995451
1115	10	0.12307696952197489
1115	11	0.09812699768586697
1115	12	0.06608038021820306
1115	13	0.0329602156756863
1115	14	0.11311441709700887
1115	15	0.015337645145898193
1115	16	0.08285159617729221
1115	17	0.13121044173279017
1115	18	0.19476278016015414
1115	19	-0.03659660112762708
1115	20	0.021250090264736343
1115	21	0.03717411916159108
1115	22	0.09608548254536942
1115	23	0.02413539747767146
1115	24	0.0653709801701794
1115	25	0.12309428108257237
1116	1	0.17984203639718999
1116	2	-0.0732215926575499
1116	3	0.2015478359966707
1116	4	0.5783892049700725
1116	5	0.23048508030730375
1116	6	0.3066302080479133
1116	7	0.08974098686601906
1116	8	0.07506160519916705
1116	9	0.07755238050766877
1116	10	0.01269798166238445
1116	11	-0.07502644985008544
1116	12	-0.0038040441901756594
1116	13	0.05693305194461118
1116	14	-0.019785849945265
1116	15	-0.003139622708339118
1116	16	-0.07072043382014925
1116	17	-0.01964988048821681
1116	18	-0.21574482385037186
1116	19	-0.29218951838797835
1116	20	-0.1607141867191686
1116	21	-0.12405129078840815
1116	22	-0.08234586055554871
1116	23	-0.20241775039063725
1116	24	-0.17404391060834712
1116	25	-0.2257001302341166
1201	1	0.4950124040737869
1201	2	0.4570896949548775
1201	3	0.5494013564867544
1201	4	0.7142993303203795
1201	5	0.5226097837019097
1201	6	0.4674392131887686
1201	7	0.4425921475525243
1201	8	0.4828579036217775
1201	9	0.5195728939560098
1201	10	0.436907067141604
1201	11	0.4358767844391096
1201	12	0.43148738883541427
1201	13	0.48764244548414126
1201	14	0.4203127728204805
1201	15	0.5040074127952039
1201	16	0.4825471968827273
1201	17	0.5615492764298509
1201	18	0.526063687597123
1201	19	0.44056546083379544
1201	20	0.7066372516019399
1201	21	0.591378564729311
1201	22	0.6020424540883056
1201	23	0.6170472424756213
1201	24	0.6901648416369071
1201	25	0.6640909936445212
1202	1	0.7051848571367287
1202	2	0.6788528126429121
1202	3	0.6918617511354412
1202	4	0.9064799230747909
1202	5	0.7526044470169353
1202	6	0.7095895481031604
1202	7	0.7353028343888681
1202	8	0.6011879202303376
1202	9	0.6841670648330052
1202	10	0.6617036164178245
1202	11	0.7728009918748882
1202	12	0.7634281133943048
1202	13	0.804866222161337
1202	14	0.8737562646417203
1202	15	0.8371091572760534
1202	16	0.864076946823289
1202	17	0.7790873522639785
1202	18	0.8826020410718698
1202	19	0.8403052766599757
1202	20	0.900079696036407
1202	21	0.8858604158441139
1202	22	0.7188181819805521
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2210	14	0.09786167074489072
2210	15	0.09214799066204904
2210	16	0.03597561186447962
2210	17	-0.027209406944798786
2210	18	0.06742365355447788
2210	19	0.09785315781014958
2210	20	0.19516939076582673
2210	21	0.012882589973145286
2210	22	0.01061405249057401
2210	23	-0.06633598569474941
2210	24	-0.022411924571429154
2210	25	-0.013466643257267208
2211	1	0.3249504652804376
2211	2	0.28021789147884135
2211	3	0.3873843036657192
2211	4	0.6697691042126905
2211	5	0.3328840282007661
2211	6	0.3092875759779403
2211	7	0.2533947705941344
2211	8	0.14519264844153668
2211	9	0.16897585749084953
2211	10	0.17430778105884315
2211	11	0.19899885630876213
2211	12	0.1924593394048344
2211	13	0.14139915434678585
2211	14	0.25377489597921965
2211	15	0.21747504266740236
2211	16	0.2104596244946677
2211	17	0.2880955854260918
2211	18	0.2683737460707576
2211	19	0.18122217552385678
2211	20	0.3038053616298271
2211	21	0.3023525294730476
2211	22	0.22127512016284356
2211	23	0.2021776299519118
2211	24	-2.954403086685076
2211	25	-2.095777736944605
2212	1	0.0938563451879233
2212	2	-0.06651536764987398
2212	3	-0.316299799769169
2212	4	0.33134095293272736
2212	5	0.1917835302571973
2212	6	0.04851138923633158
2212	7	-0.012698804048348222
2212	8	0.04065135070359105
2212	9	-0.102933290068421
2212	10	-0.08957450767730535
2212	11	-0.1496001272884513
2212	12	-0.17536289846508168
2212	13	-0.11107499750401928
2212	14	-0.17160135662611964
2212	15	-0.14421225415611616
2212	16	-0.14021726400078194
2212	17	-0.15523442399106102
2212	18	-0.23164077731383514
2212	19	-0.20436344209642954
2212	20	-0.5240879215645364
2212	21	-0.3679764445767688
2212	22	-0.2641059774728589
2212	23	-0.2079078034006372
2212	24	-0.1515811162356684
2212	25	-0.013672251517121481
2213	1	-0.17712150878172395
2213	2	-0.5145207951170754
2213	3	-0.40731783259393595
2213	4	0.26205741326513277
2213	5	-0.13103140148785286
2213	6	-0.23146569061878353
2213	7	-0.21946703473447826
2213	8	-0.19515447941870434
2213	9	-0.29026019748085474
2213	10	-0.3747514419526752
2213	11	-0.4361586971407334
2213	12	-0.42570388436348594
2213	13	-0.46697610437226444
2213	14	-0.41932183484403396
2213	15	-0.3834119121592465
2213	16	-0.38789138200125706
2213	17	-0.5223770892558477
2213	18	-0.384693113811025
2213	19	-0.41653162714446523
2213	20	-6.11142623850089
2213	21	-2.998395390741152
2213	22	-1.6467694225136142
2213	23	-0.9840316415067178
2213	24	-0.6215276358975999
2213	25	-0.5568656039529785
2214	1	0.03642934747151827
2214	2	-0.298608755677531
2214	3	-0.0054301833982925984
2214	4	0.3761466711789865
2214	5	0.09567461823191792
2214	6	4.6866329922323757E-4
2214	7	-0.1352623123681056
2214	8	-0.2221005095173041
2214	9	-0.27997218502626
2214	10	-0.31110977096149384
2214	11	-0.2903232015517361
2214	12	-0.3795570239587107
2214	13	-0.36805939446048086
2214	14	-0.3630750782275314
2214	15	-0.3196340190611693
2214	16	-0.3384816777978301
2214	17	-0.41257558432590713
2214	18	-0.41747074743842205
2214	19	-0.37752021043455386
2214	20	-0.2720645363732288
2214	21	-0.37252616396377647
2214	22	-0.3818628319583439
2214	23	-0.3867625704117543
2214	24	-0.2565886115073539
2214	25	-0.22399715558764655
2215	1	-0.02575487895764894
2215	2	0.02324252104111224
2215	3	0.020186583098841027
2215	4	0.42535379564787235
2215	5	0.11411831216936719
2215	6	-0.0689841119137995
2215	7	-0.15552434934310355
2215	8	-0.2713236898494955
2215	9	-0.36084206659213436
2215	10	-0.3957952656717154
2215	11	-0.4308507906125527
2215	12	-0.4565921971354072
2215	13	-0.48606767717159727
2215	14	-0.4631416935418642
2215	15	-0.5162332418516655
2215	16	-0.5074158552334893
2215	17	-0.46342833567317854
2215	18	-0.4942626379456314
2215	19	-0.44127995974636036
2215	20	-0.3853040294625387
2215	21	-0.43880331406521833
2215	22	-0.5211831918898326
2215	23	-0.5464632389038968
2215	24	-0.5154580223655287
2215	25	-0.6418253615504099
2216	1	-0.8726501797952793
2216	2	-0.48820091596857296
2216	3	-2.1495688274743046
2216	4	-0.5254554129551892
2216	5	-0.667516840673926
2216	6	-0.6002442951260107
2216	7	-0.6097927392943703
2216	8	-0.6043669630635762
2216	9	-0.5849718586188999
2216	10	-0.6880594259694419
2216	11	-0.7047928200444247
2216	12	-0.6445310113444265
2216	13	-0.7820565498832224
2216	14	-0.9419252318243778
2216	15	-0.9418256412369033
2216	16	-1.005549015570395
2216	17	-0.9029593453676483
2216	18	-1.0105683506549852
2216	19	-0.7983711921659378
2216	20	-0.7271496916180027
2216	21	-0.764912305064712
2216	22	-0.8850112395207717
2216	23	-0.9844982037539225
2216	24	-0.937607712230573
2216	25	-1.0272658770361076
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62616.0
3	1.0
4	1083.0
5	8058.0
6	9350.0
7	6888.0
8	5570.0
9	4697.0
10	4369.0
11	4102.0
12	3729.0
13	3436.0
14	3416.0
15	3345.0
16	3389.0
17	3742.0
18	4156.0
19	4834.0
20	6206.0
21	8117.0
22	10170.0
23	12533.0
24	14143.0
25	16379.0
26	17786.0
27	19478.0
28	22140.0
29	26209.0
30	33255.0
31	42415.0
32	53743.0
33	70255.0
34	94353.0
35	128522.0
36	188133.0
37	313526.0
38	1132954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.797912009149044	18.554184156592484	13.020701042757452	27.627202791501016
2	13.683250163603846	20.029288818876154	39.1884822892175	27.09897872830251
3	17.374008243371176	24.12340686243182	29.42403768398252	29.078547210214484
4	10.549580801483364	16.735134195504404	37.238027555730525	35.47725744728171
5	12.868363658677731	37.82214907813737	35.67088059550119	13.638606667683714
6	33.075053987597286	36.28317259307701	17.48051891394362	13.161254505382077
7	28.230503136481133	31.06869581778372	21.635946634444753	19.064854411290394
8	25.85243339608272	36.436352136980894	19.80756666358389	17.903647803352495
9	26.47350016088922	14.750280757010401	19.111934144101333	39.66428493799905
10	14.44387067306791	29.10772017908484	34.34724700516906	22.101162142678195
11	34.76133849333646	22.345546088732682	23.220653192315215	19.672462225615643
12	23.21598173574351	24.889353051435545	31.073608822610794	20.821056390210146
13	29.270284918591038	20.925716983479596	25.113297621218656	24.690700476710713
14	21.24153516183211	21.45495091309901	27.0744445609021	30.229069364166776
15	24.51061886236487	28.95282981487344	22.835996978395013	23.70055434436668
16	22.612262359682703	27.53022808698053	27.160323910101482	22.697185643235283
17	22.767039258277197	27.853385648724053	26.93467011174339	22.444904981255355
18	22.696129110648304	27.024388372197127	28.31255315838053	21.96692935877404
19	23.628712483080523	26.88569080910055	27.361183562279024	22.124413145539908
20	23.851240979285908	26.824614907430366	27.26192089124527	22.062223222038448
21	23.209725371501317	26.40026108837381	27.149398959907085	23.240614580217784
22	23.236140970679536	27.147865150922545	26.226514615069334	23.389479263328585
23	22.4121028218359	26.99382085638849	26.90055639134264	23.693519930432974
24	23.00246432606817	27.397392177213337	27.063020963813944	22.537122532904547
25	23.147828998971924	26.261803410766575	26.644787156218936	23.945580434042565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	245.0
2	371.0
3	497.0
4	497.0
5	497.0
6	1128.5
7	1760.0
8	1760.0
9	1760.0
10	2531.5
11	3303.0
12	3303.0
13	3303.0
14	5796.5
15	8290.0
16	8290.0
17	8290.0
18	15257.5
19	22225.0
20	22225.0
21	22225.0
22	35602.5
23	48980.0
24	48980.0
25	48980.0
26	70276.5
27	91573.0
28	91573.0
29	91573.0
30	118952.5
31	146332.0
32	146332.0
33	146332.0
34	169829.0
35	193326.0
36	193326.0
37	193326.0
38	215505.0
39	237684.0
40	237684.0
41	237684.0
42	256923.0
43	276162.0
44	276162.0
45	276162.0
46	310630.5
47	345099.0
48	345099.0
49	345099.0
50	333474.0
51	321849.0
52	321849.0
53	321849.0
54	284117.5
55	246386.0
56	246386.0
57	246386.0
58	212135.5
59	177885.0
60	177885.0
61	177885.0
62	145344.5
63	112804.0
64	112804.0
65	112804.0
66	88000.0
67	63196.0
68	63196.0
69	63196.0
70	46836.0
71	30476.0
72	30476.0
73	30476.0
74	21334.5
75	12193.0
76	12193.0
77	12193.0
78	8382.5
79	4572.0
80	4572.0
81	4572.0
82	3072.0
83	1572.0
84	1572.0
85	1572.0
86	1049.0
87	526.0
88	526.0
89	526.0
90	338.5
91	151.0
92	151.0
93	151.0
94	81.0
95	11.0
96	11.0
97	11.0
98	6.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19423986557016365
2	0.06361046705335695
3	0.0
4	0.0
5	0.018576131035005782
6	0.38605120024813616
7	0.001746838010172562
8	0.030974420326718356
9	0.032508229311260115
10	0.0023007134768126426
11	0.0030250121639573634
12	0.00933067132262905
13	0.0077542565329611285
14	0.001533808984541762
15	4.260580512616005E-5
16	0.011673990604567855
17	0.02390185667577579
18	0.06446258315588016
19	0.029398005537050437
20	0.0
21	0.0
22	0.0
23	3.834522461354405E-4
24	2.556348307569603E-4
25	4.260580512616005E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2347098.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.5389098846603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76032384159002	46.62207741720291
2	14.462551362249329	17.800192899674652
3	4.8670499612334375	8.985388469054516
4	2.0503628226236095	5.047083718891683
5	1.0059804980127751	3.095347160646693
6	0.557854513793785	2.059785519186404
7	0.3319226988489511	1.429831273719719
8	0.21891037034844466	1.0777204442952437
9	0.1432059384962005	0.79314636096593
>10	0.5601062549500615	5.638767496005345
>50	0.023595491561425742	1.0047561466109611
>100	0.013902509928638543	1.6932351251906521
>500	0.001873948882119878	0.8101497746792518
>1k	0.0021515709387260767	2.5854489830551697
>5k	6.940551415245408E-5	0.21816981490171986
>10k+	1.3881102830490816E-4	1.1388993959190212
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	13683	0.582975231541248	No Hit
CCCATGTACTCTGCGTTGATACCAC	12982	0.5531085621478098	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5108	0.21763045258442554	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4434	0.18891413992939365	No Hit
CATGTACTCTGCGTTGATACCACTG	3758	0.16011261566410948	No Hit
GTATCAACGCAGAGTACATGGGAAG	3517	0.14984461662870488	No Hit
GCGTTGATACCACTGCTTCCCATGT	3357	0.1430276878085193	No Hit
TATCAACGCAGAGTACATGGGAAGC	3162	0.1347195558089181	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3131	0.13339877585000712	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2966	0.12636881800419072	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2633	0.11218108489717941	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2505	0.10672754184103093	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2400	0.10225393230278412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.704232205046402E-4	0.0	0.0	0.0
6	0.0	1.704232205046402E-4	0.0	0.0	0.0
7	0.0	1.704232205046402E-4	0.0	0.0	0.0
8	4.260580512616005E-5	1.704232205046402E-4	0.0	0.0	4.260580512616005E-5
9	4.260580512616005E-5	1.704232205046402E-4	0.0	0.0	4.260580512616005E-5
10	4.260580512616005E-5	1.704232205046402E-4	0.0	4.260580512616005E-5	4.260580512616005E-5
11	4.260580512616005E-5	1.704232205046402E-4	0.0	4.260580512616005E-5	4.260580512616005E-5
12	4.260580512616005E-5	1.704232205046402E-4	0.0	4.260580512616005E-5	4.260580512616005E-5
13	4.260580512616005E-5	1.704232205046402E-4	0.0	4.260580512616005E-5	4.260580512616005E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	110	2.8067006E-9	12.936559	19
AGGACCG	155	1.8189894E-12	12.284237	5
TATATCG	55	0.0030206048	12.116724	5
TAGGACC	500	0.0	11.995557	4
GGCGAGG	480	0.0	11.463228	19
GCACCGT	120	1.2391138E-7	11.1069975	6
TGGTCCG	60	0.0057845977	11.1069975	5
TGGCGAG	1100	0.0	11.043198	18
CTCGAAC	165	8.185452E-11	10.928164	18
CCGCTAG	80	3.8209552E-4	10.672661	9
TGAACCG	90	9.315254E-5	10.578093	5
TCGAACT	180	3.6379788E-11	10.540899	19
TCCAACG	155	3.7271093E-9	10.408659	18
TGTAGGA	1155	0.0	10.392209	2
GCGTGCG	120	1.5518854E-6	10.277377	9
GTCCTAA	650	0.0	10.262268	1
TTAGGAC	770	0.0	10.262124	3
GGACCGC	130	3.7325844E-7	10.252612	6
GTCCTAC	1150	0.0	10.10929	1
ATGGCGA	1245	0.0	9.987425	17
>>END_MODULE