FastQCFastQC Report
Thu 26 May 2016
SRR1513020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393687
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60110.2511188806222367No Hit
CCCATGTACTCTGCGTTGATACCAC51270.21418840474966028No Hit
GTATCAACGCAGAGTACTTTTTTTT27850.11634770962118271No Hit
GAGTACATGGGAAGCAGTGGTATCA24690.10314631779342913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT2850.012.3349214
GGGCGCG550.00307160412.0896857
GTGTTAC2100.011.32305051
TATAAGG2800.011.1974012
TAATACG600.00587160211.084764
CGAAATC803.7787238E-410.68552513
ACGAAAT803.7787238E-410.68552512
CTACACG1355.680704E-810.5569144
TAGGACC2250.010.5569144
GACGAAA909.5389485E-510.55338511
TGGACCG1303.8379585E-710.2318735
TACACGG1303.8379585E-710.2318735
AGACGAA856.6018547E-410.05780510
TTAGGAC3600.010.0280213
AAGACCG1151.0255659E-59.9141125
GTATAAG3750.09.8918181
GTCTAGG3000.09.8284081
CCTACAC3100.09.8066893
GTCTTAG2450.09.7054731
GTCCTAG3400.09.5113631
Produced by FastQC (version 0.11.5)