FastQCFastQC Report
Thu 26 May 2016
SRR1513020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393687
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC72160.3014596311046515No Hit
CCCATGTACTCTGCGTTGATACCAC65950.27551638956973074No Hit
GTATCAACGCAGAGTACTTTTTTTT43630.18227111564711676No Hit
TATCAACGCAGAGTACTTTTTTTTT30640.12800336886150948No Hit
GAGTACATGGGAAGCAGTGGTATCA25830.1079088452249605No Hit
GGTATCAACGCAGAGTACTTTTTTT25650.10715686720945553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC1155.4005795E-912.3801113
CGTTTAT600.005738781811.1188811
CGGTACT600.005777459611.1088534
TAGCCCG700.001463555410.8821435
TATACCG909.29965E-510.5798625
TCGAACT1553.752575E-910.40450519
TAGGACC2900.010.1785564
TAGAACG750.002598404210.1566674
CTAGCAC2350.010.1296543
CTACACT3600.010.0508684
GTCTTAG2400.09.9275721
CTAGGAC3650.09.9131853
TGGCGAG4200.09.7151718
ACACCGC800.00443204969.5218756
TCCGGAC1002.6932865E-49.5218743
TTAGGGC1201.6553646E-59.5218743
GGACCGC1106.668875E-59.5218746
TAGACAG4150.09.4071545
GTATTAA3150.09.3791921
CTCGAAC1951.717126E-99.24592218
Produced by FastQC (version 0.11.5)