FastQCFastQC Report
Thu 26 May 2016
SRR1513019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957596
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52840.26992290544116354No Hit
CCCATGTACTCTGCGTTGATACCAC44800.22885212270560423No Hit
GTATCAACGCAGAGTACTTTTTTTT28710.14665947417138164No Hit
GTCCTACAGTGGACATTTCTAAATT22380.11432389522659425No Hit
GAGTACATGGGAAGCAGTGGTATCA21730.11100349612483884No Hit
GTCCTAAAGTGTGTATTTCTCATTT21080.10768309702308343No Hit
CTGTAGGACGTGGAATATGGCAAGA21050.10752984783377163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG655.4683696E-513.1501315
ATGCCGT550.003081914512.0840976
GTCTAGG2200.011.6919531
TGTCGAG752.0684603E-411.40348318
CCCGGTC600.005901281711.0770888
GGCCGCA700.001498731310.8510257
TTAGACA1951.8189894E-1210.7151964
TAGGACC3750.010.6372664
ATAGGAG1909.094947E-1210.4975713
GTCCTAC12850.010.3052281
TAGACAG2700.010.2008215
CCAACGA951.6536939E-49.99436619
AAGGCGT2151.0913936E-119.7154546
GGACGTG12600.09.6453556
TCCTACA14950.09.5930982
GTCCTAG3200.09.5267761
CATTCGT800.00453737569.49464719
TGGCGAG11750.09.38158918
GTAGGAC12150.09.3805463
GACGTGG6800.09.35502057
Produced by FastQC (version 0.11.5)