FastQCFastQC Report
Thu 26 May 2016
SRR1513019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957596
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC53510.27334547066912684No Hit
GTACATGGGAAGCAGTGGTATCAAC53000.2707402344508264No Hit
GTATCAACGCAGAGTACTTTTTTTT34150.17444866049991928No Hit
TATCAACGCAGAGTACTTTTTTTTT24910.1272479101918884No Hit
CTGTAGGACGTGGAATATGGCAAGA21970.11222948963933313No Hit
GGTATCAACGCAGAGTACTTTTTTT21570.11018616711517595No Hit
GTCCTACAGTGGACATTTCTAAATT21310.1088580074744738No Hit
CTTTAGGACGTGAAATATGGCGAGG20500.1047202793630555No Hit
GAGTACATGGGAAGCAGTGGTATCA19680.10053146818853329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG704.359772E-714.9519335
GTACCGA550.00304018812.106116
TAGGACC4450.011.7599494
GTACCGT600.005821873411.0972676
GTCCTAC12100.011.0784931
CTAACAC1408.247298E-910.8660633
TCCTACA13850.010.7080562
GGCGAGG5800.010.4715419
GTATTAC1553.61797E-910.42711451
TGTACCG1105.934844E-610.3798555
ACCGTGC1054.0859944E-59.9567368
ATAATAC2300.09.9211883
CTATACT2500.09.8954614
CCTACAG14250.09.8747743
AATCCCG1752.6211637E-99.76097319
TCTAGAC1951.6189006E-109.75159553
GACGTGA7750.09.6886057
TTAGGAC8850.09.6689553
GTCCTAA6700.09.64897251
AGGACGT13050.09.5513225
Produced by FastQC (version 0.11.5)