FastQCFastQC Report
Thu 26 May 2016
SRR1513018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501034
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC30350.2021939543008353No Hit
GTATCAACGCAGAGTACTTTTTTTT28540.19013559985982995No Hit
GTCCTACAGTGGACATTTCTAAATT26290.17514593273703327No Hit
GTCCTAAAGTGTGTATTTCTCATTT25230.16808413400362684No Hit
CCCATGTACTCTGCGTTGATACCAC24520.16335406126709986No Hit
CTTTAGGACGTGAAATATGGCGAGG23640.1574914359035172No Hit
CTGTAGGACGTGGAATATGGCAAGA23520.15669198699030135No Hit
GGTATCAACGCAGAGTACTTTTTTT17750.11825181841317385No Hit
GTCCTACAGTGTGCATTTCTCATTT17500.11658629984397423No Hit
TATCAACGCAGAGTACTTTTTTTTT16750.11158974413637533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT655.473914E-513.1482856
TAGGACC5050.011.8496344
GTATAGG1501.6916601E-1011.42946151
GGCGAGG6250.011.24324519
GTATTAA2400.010.715121
GGACGGG803.7852698E-410.68298156
CACCTTT4250.010.50739414
AGGACGT13750.010.5001785
GTCTTAC1658.8766683E-1010.3904191
GTCCTAA8900.010.3806911
TTAGGAC9350.010.3620193
GGACGTG14050.010.1380626
AGGACCT9700.010.0860545
GTATTAG1803.7471182E-1010.0536931
TAGGACT2650.010.0361684
GTAGGAC15450.09.959613
TTAGTAC1151.0279688E-59.9114953
GTCATAA1252.4854016E-69.9055341
GACGTGA8250.09.8988447
TATGGCG15500.09.86850516
Produced by FastQC (version 0.11.5)