##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513018_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1501034 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.259696982213594 31.0 30.0 34.0 24.0 34.0 2 29.26325319746255 31.0 30.0 34.0 23.0 34.0 3 29.50836956391394 31.0 30.0 34.0 25.0 34.0 4 32.962940213213024 35.0 35.0 37.0 26.0 37.0 5 33.08741174417102 37.0 35.0 37.0 27.0 37.0 6 33.09299589482983 37.0 35.0 37.0 26.0 37.0 7 32.86396710534205 37.0 35.0 37.0 26.0 37.0 8 32.89574853067952 37.0 35.0 37.0 26.0 37.0 9 34.233734212549486 39.0 35.0 39.0 25.0 39.0 10 34.12831354919342 38.0 35.0 39.0 25.0 39.0 11 34.197200729630374 39.0 35.0 39.0 25.0 39.0 12 34.12263946053187 39.0 35.0 39.0 25.0 39.0 13 34.129805187624 39.0 35.0 39.0 25.0 39.0 14 35.12718832484807 39.0 35.0 41.0 25.0 41.0 15 35.08555169303293 40.0 35.0 41.0 25.0 41.0 16 35.0008280958326 39.0 35.0 41.0 24.0 41.0 17 34.921081734324474 39.0 35.0 41.0 23.0 41.0 18 34.652173768215775 39.0 34.0 41.0 19.0 41.0 19 34.881111953493395 39.0 34.0 41.0 22.0 41.0 20 34.963499827452274 40.0 35.0 41.0 23.0 41.0 21 34.900178143866164 40.0 35.0 41.0 22.0 41.0 22 34.80754399966956 39.0 35.0 41.0 21.0 41.0 23 34.562901306699246 39.0 34.0 41.0 18.0 41.0 24 34.57296503610178 39.0 34.0 41.0 18.0 41.0 25 34.48134152857297 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5074391699623781 1101 2 0.6742320405780546 1101 3 0.6262298548751772 1101 4 0.6620575760538401 1101 5 0.3941354947517297 1101 6 0.49388745076676344 1101 7 0.6036383762181359 1101 8 0.47670328648747784 1101 9 0.3854910136498333 1101 10 0.49311859583880846 1101 11 0.6830881403018054 1101 12 0.8948815561180226 1101 13 0.6232089015906794 1101 14 0.5780765512376718 1101 15 0.3702490755024286 1101 16 0.8532957054021324 1101 17 0.9454331316484215 1101 18 1.001762471033075 1101 19 0.622076449552921 1101 20 0.742777321165299 1101 21 0.6442152443823659 1101 22 0.8370487679609653 1101 23 1.0007214230616341 1101 24 0.5959307331579282 1101 25 0.8813228853681636 1102 1 0.6681714604587334 1102 2 0.7200290042516038 1102 3 0.8367561706646498 1102 4 0.8056925254964185 1102 5 0.7761537588026641 1102 6 0.5087449969310285 1102 7 0.7992711362792093 1102 8 0.742518687057867 1102 9 0.8013006526899602 1102 10 0.9721754333348471 1102 11 0.9799077755969421 1102 12 1.0289163557608134 1102 13 0.7653458531650159 1102 14 0.8391026797774117 1102 15 0.9330569572758307 1102 16 0.9792211512005409 1102 17 0.8640515166914895 1102 18 1.2306104530571034 1102 19 1.191077976358443 1102 20 1.0097810373523188 1102 21 0.9205526395945327 1102 22 1.117181571233516 1102 23 1.3545009869137985 1102 24 1.2476030173165995 1102 25 1.0563293670897025 1103 1 -0.02622629864490733 1103 2 0.09183910760329184 1103 3 0.08735020138038507 1103 4 0.15952233328160048 1103 5 -0.2509398372564462 1103 6 0.08027498823789614 1103 7 -0.0414610186517308 1103 8 -0.048561193170087336 1103 9 0.25241627135121547 1103 10 0.2331142916367881 1103 11 0.11469962337053374 1103 12 0.35555114760858686 1103 13 0.3768401060385784 1103 14 0.4458736640331864 1103 15 0.6251220591786577 1103 16 0.5585653026542232 1103 17 0.23744087243378686 1103 18 0.5011214160629933 1103 19 0.5554313038963556 1103 20 0.4385456466443145 1103 21 0.27482676303701226 1103 22 0.42707725364441274 1103 23 0.7184389759024938 1103 24 0.5492933486215392 1103 25 0.5655247693885528 1104 1 0.5873572798136415 1104 2 0.5453923905419238 1104 3 0.648308765979305 1104 4 0.8357111633984076 1104 5 0.6291882257217054 1104 6 0.6321333199950345 1104 7 0.8077109907662958 1104 8 0.7640202525360422 1104 9 0.8072464338186904 1104 10 0.9382490398480812 1104 11 0.9032587436590731 1104 12 0.8387628966612368 1104 13 0.9119576356981867 1104 14 0.9785015150405343 1104 15 1.0989276852099792 1104 16 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-0.14607465677782727 2216 17 -0.3924526366940029 2216 18 -0.6198724987443072 2216 19 -0.9544064242983694 2216 20 -0.7849077002116758 2216 21 -1.023068774488344 2216 22 -1.560346681446866 2216 23 -1.2604910443814816 2216 24 -1.1093526960032136 2216 25 -1.197888009516106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66156.0 3 0.0 4 527.0 5 3359.0 6 4288.0 7 3880.0 8 3354.0 9 3227.0 10 2922.0 11 2835.0 12 2612.0 13 2675.0 14 2579.0 15 2552.0 16 2790.0 17 2859.0 18 3332.0 19 4076.0 20 5159.0 21 6646.0 22 8329.0 23 9975.0 24 11393.0 25 13247.0 26 14645.0 27 16545.0 28 18841.0 29 21857.0 30 27288.0 31 34344.0 32 43439.0 33 55630.0 34 73221.0 35 96780.0 36 134885.0 37 212967.0 38 581820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99509447006303 19.138787651463335 12.618584851226114 25.24753302724752 2 14.487754972824005 20.648758168627804 41.558512534948896 23.304974323599296 3 18.23888514931988 25.44769305617573 30.20960099168927 26.103820802815118 4 11.997091581848911 17.375665790497923 38.94141310201057 31.6858295256426 5 12.594583678521133 38.45044391761881 36.27667721280653 12.678295191053532 6 30.886653717628793 37.712218183514295 18.76908622659928 12.632041872257629 7 27.22149259141541 31.94516907777998 23.06832742632228 17.76501090448233 8 24.462307552882976 37.34866779682702 21.25414480979188 16.934879840498123 9 26.416762732306708 15.332209420874118 20.60764571436187 37.643382132457305 10 14.685321674952673 29.196460639305187 35.15132372862947 20.966893957112674 11 33.312832254083666 22.726479176189784 25.555828014824744 18.4048605549018 12 23.0402945780134 25.140215749342147 32.33241577559616 19.48707389704829 13 28.545488710782262 22.46502354863386 26.095138080020043 22.894349660563833 14 20.458092487475128 22.83212071858586 28.149344201773406 28.560442592165607 15 22.65244841084202 31.61249521859835 23.55375095128235 22.18130541927728 16 21.576806618760262 28.382212614879414 28.544603066294222 21.496377700066102 17 21.358989104338075 29.062917100078696 28.220037596064206 21.35805619951902 18 21.278068209775096 27.729147734508185 30.348436167595523 20.644347888121196 19 23.002708835200863 27.403816358965642 28.05953440020791 21.533940405625582 20 23.161335979241436 27.874859563851633 28.37489887930954 20.588905577597387 21 22.423440743418617 27.3064402736175 27.69187710535873 22.578241877605144 22 22.75805774523065 28.677510278331216 27.383806547480226 21.180625428957907 23 21.67482071532619 28.48943749725126 28.091486160329342 21.74425562709321 24 22.560594904530753 27.603756800788943 28.671759692685967 21.163888601994344 25 22.247669894308125 27.72264362293702 28.22947951032963 21.800206972425222 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 417.0 3 595.0 4 595.0 5 595.0 6 1148.0 7 1701.0 8 1701.0 9 1701.0 10 2504.5 11 3308.0 12 3308.0 13 3308.0 14 5513.5 15 7719.0 16 7719.0 17 7719.0 18 13821.5 19 19924.0 20 19924.0 21 19924.0 22 31195.0 23 42466.0 24 42466.0 25 42466.0 26 60257.5 27 78049.0 28 78049.0 29 78049.0 30 99525.5 31 121002.0 32 121002.0 33 121002.0 34 136869.0 35 152736.0 36 152736.0 37 152736.0 38 163378.5 39 174021.0 40 174021.0 41 174021.0 42 181300.0 43 188579.0 44 188579.0 45 188579.0 46 194031.0 47 199483.0 48 199483.0 49 199483.0 50 188494.5 51 177506.0 52 177506.0 53 177506.0 54 154815.5 55 132125.0 56 132125.0 57 132125.0 58 112358.0 59 92591.0 60 92591.0 61 92591.0 62 74743.0 63 56895.0 64 56895.0 65 56895.0 66 43660.5 67 30426.0 68 30426.0 69 30426.0 70 21917.5 71 13409.0 72 13409.0 73 13409.0 74 9399.5 75 5390.0 76 5390.0 77 5390.0 78 3650.5 79 1911.0 80 1911.0 81 1911.0 82 1280.0 83 649.0 84 649.0 85 649.0 86 426.5 87 204.0 88 204.0 89 204.0 90 139.5 91 75.0 92 75.0 93 75.0 94 47.5 95 20.0 96 20.0 97 20.0 98 15.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3580864923779208 2 0.04190444720106273 3 0.037574098921143696 4 0.03744085743560772 5 0.04283713759981453 6 0.01858718723226789 7 0.01832070426119595 8 0.024116708882010666 9 0.021585120656827227 10 0.020719051000843418 11 0.01998622283039558 12 0.020719051000843418 13 0.020652430258075435 14 0.027980711962553813 15 0.028513677904697696 16 0.02198484511343514 17 0.022984156254954917 18 0.0773466823536309 19 0.025915468936746268 20 0.023450501454330815 21 0.02658167636442612 22 0.02025270580146752 23 0.023184018483258875 24 0.019253394659947742 25 0.02338388071156283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1501034.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.10666516507162 #Duplication Level Percentage of deduplicated Percentage of total 1 84.88898731516024 62.908397591665924 2 10.715013506306995 15.88107836302225 3 2.4840757820511934 5.522597166753936 4 0.8173149416693751 2.4227393886680972 5 0.34927117446450107 1.2941660988926047 6 0.18457986257085357 0.8207158843051908 7 0.11822189833562828 0.613272144459628 8 0.08661319452373085 0.513489200435787 9 0.056306158737705586 0.37553954870758677 >10 0.26363355763261775 3.446041899867082 >50 0.017247845496329385 0.8870415621022415 >100 0.015312190955661933 2.3507242293018162 >500 0.002251692167818559 1.169259213609664 >1k 0.0011708799272656504 1.7949377082081903 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3035 0.2021939543008353 No Hit GTATCAACGCAGAGTACTTTTTTTT 2854 0.19013559985982995 No Hit GTCCTACAGTGGACATTTCTAAATT 2629 0.17514593273703327 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2523 0.16808413400362684 No Hit CCCATGTACTCTGCGTTGATACCAC 2452 0.16335406126709986 No Hit CTTTAGGACGTGAAATATGGCGAGG 2364 0.1574914359035172 No Hit CTGTAGGACGTGGAATATGGCAAGA 2352 0.15669198699030135 No Hit GGTATCAACGCAGAGTACTTTTTTT 1775 0.11825181841317385 No Hit GTCCTACAGTGTGCATTTCTCATTT 1750 0.11658629984397423 No Hit TATCAACGCAGAGTACTTTTTTTTT 1675 0.11158974413637533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCGT 65 5.473914E-5 13.148285 6 TAGGACC 505 0.0 11.849634 4 GTATAGG 150 1.6916601E-10 11.4294615 1 GGCGAGG 625 0.0 11.243245 19 GTATTAA 240 0.0 10.71512 1 GGACGGG 80 3.7852698E-4 10.6829815 6 CACCTTT 425 0.0 10.507394 14 AGGACGT 1375 0.0 10.500178 5 GTCTTAC 165 8.8766683E-10 10.390419 1 GTCCTAA 890 0.0 10.380691 1 TTAGGAC 935 0.0 10.362019 3 GGACGTG 1405 0.0 10.138062 6 AGGACCT 970 0.0 10.086054 5 GTATTAG 180 3.7471182E-10 10.053693 1 TAGGACT 265 0.0 10.036168 4 GTAGGAC 1545 0.0 9.95961 3 TTAGTAC 115 1.0279688E-5 9.911495 3 GTCATAA 125 2.4854016E-6 9.905534 1 GACGTGA 825 0.0 9.898844 7 TATGGCG 1550 0.0 9.868505 16 >>END_MODULE