##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513018_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501034
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.377085395800496	31.0	31.0	34.0	26.0	34.0
2	30.54650261086691	31.0	31.0	34.0	27.0	34.0
3	30.633101581976156	31.0	31.0	34.0	27.0	34.0
4	34.10015029639568	37.0	35.0	37.0	30.0	37.0
5	33.76386744071087	37.0	35.0	37.0	28.0	37.0
6	33.635450629366154	37.0	35.0	37.0	28.0	37.0
7	33.500774133030966	37.0	35.0	37.0	28.0	37.0
8	33.372611146716196	37.0	35.0	37.0	27.0	37.0
9	34.81271043827122	39.0	35.0	39.0	27.0	39.0
10	34.69007830602105	39.0	35.0	39.0	27.0	39.0
11	34.72833393514071	39.0	35.0	39.0	27.0	39.0
12	34.58620990597149	39.0	35.0	39.0	27.0	39.0
13	34.57096574761131	39.0	35.0	39.0	27.0	39.0
14	35.61440313810347	40.0	36.0	41.0	27.0	41.0
15	35.61670555097353	40.0	36.0	41.0	27.0	41.0
16	35.59277737879355	40.0	36.0	41.0	27.0	41.0
17	35.571220905056116	40.0	36.0	41.0	27.0	41.0
18	35.50542825812074	40.0	36.0	41.0	27.0	41.0
19	35.48871511238253	40.0	36.0	41.0	26.0	41.0
20	35.48191713179049	40.0	36.0	41.0	26.0	41.0
21	35.41508053781593	40.0	36.0	41.0	26.0	41.0
22	35.392326223123526	40.0	36.0	41.0	26.0	41.0
23	35.270374954864444	40.0	36.0	41.0	25.0	41.0
24	35.249408074700504	40.0	36.0	41.0	25.0	41.0
25	35.14600735226517	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8422920105254725
1101	2	0.9256415647497
1101	3	0.9609389918678843
1101	4	0.6696270503476001
1101	5	0.9965832772748158
1101	6	1.0825061239833431
1101	7	1.1034235531479482
1101	8	1.086064252822318
1101	9	0.9488537537687165
1101	10	1.2814842037049274
1101	11	1.3443658570964487
1101	12	1.3164079065826044
1101	13	1.4416018915894924
1101	14	1.4812164406028572
1101	15	1.5655869369090425
1101	16	1.703331384285221
1101	17	1.5174028996403734
1101	18	0.921088019184559
1101	19	1.3414217452679438
1101	20	1.2950913779709126
1101	21	1.42306412526127
1101	22	1.3741987796454183
1101	23	1.2172850911279198
1101	24	1.139837886910236
1101	25	1.3195352100972855
1102	1	0.843826017957845
1102	2	0.8152506449213952
1102	3	0.8104118118488692
1102	4	0.7693591391903993
1102	5	1.0164749605058816
1102	6	0.8135578913327493
1102	7	0.9752367067215317
1102	8	1.0037103796336027
1102	9	1.226905204233958
1102	10	1.2407501847494942
1102	11	1.166701869541349
1102	12	1.2239209420448276
1102	13	1.3751282361866188
1102	14	1.4442058105795255
1102	15	1.4025500343077084
1102	16	1.335688138239071
1102	17	1.231054845597221
1102	18	1.3788776080993728
1102	19	1.4295155429806172
1102	20	1.377718923847091
1102	21	1.4069246386136172
1102	22	1.276036707799129
1102	23	1.6705950902636957
1102	24	1.5404947013601529
1102	25	1.3667797582492796
1103	1	0.5971777034596322
1103	2	0.5389442602985817
1103	3	0.5527135601343929
1103	4	0.5150463796038096
1103	5	0.6330208892221947
1103	6	0.700701714379214
1103	7	0.6503308823228124
1103	8	0.6956692321401192
1103	9	0.731531882491467
1103	10	0.9495688846432486
1103	11	0.7715941712896353
1103	12	0.9248385504675056
1103	13	0.8926980227219232
1103	14	1.002995533365187
1103	15	0.8627161697273991
1103	16	0.9381994876064041
1103	17	1.088815881045882
1103	18	1.0088737444849656
1103	19	1.0527262065563932
1103	20	1.137242733370904
1103	21	0.8645262999678138
1103	22	1.0171648832072293
1103	23	1.0542664898580156
1103	24	1.0291549930630453
1103	25	1.2117232528885111
1104	1	0.7492287655964276
1104	2	0.8977576803334095
1104	3	0.8254666695830721
1104	4	0.7804356232267438
1104	5	0.8682996797493772
1104	6	0.9636035621124321
1104	7	0.9648083253678834
1104	8	0.8588812094690255
1104	9	1.1035135812581274
1104	10	1.2037641130173284
1104	11	1.2006454225696146
1104	12	1.0841690162162863
1104	13	1.1175760579808198
1104	14	1.3264081981317233
1104	15	1.281778541322005
1104	16	1.38781288546582
1104	17	1.263774791320266
1104	18	1.4158676397257324
1104	19	1.2521558420318257
1104	20	1.4606516478847098
1104	21	1.3914487121303765
1104	22	1.502112347058052
1104	23	1.3594602637862536
1104	24	1.4205184871246033
1104	25	1.4987214527671995
1105	1	0.5716137899639016
1105	2	0.6299609914851736
1105	3	0.5178874834710285
1105	4	0.5133360585329712
1105	5	0.5693640158910398
1105	6	0.7088421959648414
1105	7	0.7153534388626355
1105	8	0.8723506615376166
1105	9	0.8536221136001103
1105	10	0.8502420750676265
1105	11	0.8159056255904744
1105	12	0.7418287516327382
1105	13	0.9031480234230784
1105	14	0.9856872557048675
1105	15	0.9805130280620986
1105	16	1.1223883801706194
1105	17	0.8325295396102277
1105	18	1.0885525664140943
1105	19	0.9104080921164979
1105	20	1.0524283288610832
1105	21	0.9353946669469195
1105	22	1.0034036204039438
1105	23	0.9484098733967272
1105	24	0.9320976130861922
1105	25	1.1319221826798866
1106	1	0.7860425562931752
1106	2	0.6847242266842066
1106	3	0.7317546975317235
1106	4	0.657656294463095
1106	5	0.8185122821391317
1106	6	0.8274833046201593
1106	7	0.9345055633396271
1106	8	0.9335074168664548
1106	9	0.9386446774803403
1106	10	0.9889379936998708
1106	11	0.8835431957173299
1106	12	1.0864107738495576
1106	13	0.970772401631784
1106	14	1.2837293307577582
1106	15	1.2203987762791968
1106	16	1.290925720546376
1106	17	1.2346348394551967
1106	18	1.3059123815187732
1106	19	1.2454059415081105
1106	20	1.2998295617028361
1106	21	1.3297185265829583
1106	22	1.2025103809688389
1106	23	1.3319093570389953
1106	24	1.406090723647658
1106	25	1.4291432644074078
1107	1	0.7363741259263321
1107	2	0.7274893490043439
1107	3	0.6664246160406755
1107	4	0.7122476467825081
1107	5	0.7367107495134633
1107	6	0.6988644354924531
1107	7	0.8238834106568831
1107	8	0.7901442092677371
1107	9	0.6657255212022832
1107	10	0.8988468988322609
1107	11	0.9514383135184019
1107	12	0.904009870293379
1107	13	0.9333743940065844
1107	14	1.1225881282489354
1107	15	1.1189436432817175
1107	16	1.1393864094886723
1107	17	1.0255881567907394
1107	18	1.0992886706258957
1107	19	1.260920867605627
1107	20	1.21148582851675
1107	21	1.1931001704303767
1107	22	1.0953250973122408
1107	23	1.2524432111470745
1107	24	1.1180347836464577
1107	25	1.1108158461443338
1108	1	0.5322707742286212
1108	2	0.4580922844242039
1108	3	0.5336493938269804
1108	4	0.5824721401713973
1108	5	0.6559734830691966
1108	6	0.625141337972515
1108	7	0.6545245881782691
1108	8	0.7475621451358023
1108	9	0.6355577912160228
1108	10	0.6526538581208641
1108	11	0.7170150372988644
1108	12	0.7910837820218433
1108	13	0.7626111577574619
1108	14	0.8773403381329388
1108	15	0.987003072668287
1108	16	0.962835105420119
1108	17	0.840054311886
1108	18	0.866358319617575
1108	19	0.9602557258938091
1108	20	0.9605591185692219
1108	21	1.0694032555296928
1108	22	1.0811889012598854
1108	23	0.9603354651545928
1108	24	1.0643725059004652
1108	25	1.1056257545245103
1109	1	0.30663187252615387
1109	2	0.3594761512158655
1109	3	0.3811562015587775
1109	4	0.531219302583132
1109	5	0.40197638968546556
1109	6	0.3422387889791665
1109	7	0.5603560077412197
1109	8	0.6248247342728561
1109	9	0.6895357055617808
1109	10	0.5841668362121197
1109	11	0.7561391174077201
1109	12	0.7950211062484414
1109	13	0.6288694431845343
1109	14	0.9063812043641093
1109	15	0.9486000251852857
1109	16	0.7588590924445029
1109	17	0.7737072407400376
1109	18	0.7954003432044843
1109	19	0.9045363218328148
1109	20	0.8328153174492385
1109	21	0.9400603194504527
1109	22	0.9372360023317583
1109	23	0.7826924366522263
1109	24	0.38675146715715414
1109	25	0.5897013662283541
1110	1	0.3060056424313231
1110	2	0.29240690805966807
1110	3	0.33888567015781135
1110	4	0.4289559280975297
1110	5	0.26400000750619057
1110	6	0.5005318646635502
1110	7	0.43911786138230013
1110	8	0.49949820000912837
1110	9	0.6103444369985311
1110	10	0.5793090799050944
1110	11	0.5484812179623759
1110	12	0.5334269033498913
1110	13	0.23312780782936926
1110	14	0.6275030508482757
1110	15	0.6762167723672547
1110	16	0.7227882389823463
1110	17	0.6492663747532745
1110	18	0.5289573301431147
1110	19	0.682170961303207
1110	20	0.5741465037079578
1110	21	0.6536405663640323
1110	22	0.7161678327128627
1110	23	0.6157752779476269
1110	24	0.5041696099376125
1110	25	0.5807104912954557
1111	1	-0.22922864076491578
1111	2	-0.08946341334869956
1111	3	-0.03560430895173283
1111	4	0.0628398119814193
1111	5	0.23214614277188872
1111	6	0.07251281300319334
1111	7	0.33074862257827675
1111	8	0.5196827867697991
1111	9	0.09888324754820843
1111	10	0.23527210815073119
1111	11	0.29216715933853976
1111	12	0.14949745759979294
1111	13	0.2497198074376712
1111	14	0.4208289854785576
1111	15	0.6540492243844866
1111	16	0.3529156490010621
1111	17	0.5246839118555968
1111	18	0.4717982689079676
1111	19	0.3525471799131168
1111	20	0.4942819924575801
1111	21	0.33002007086853524
1111	22	0.20118953920196248
1111	23	0.28623173984431105
1111	24	0.3042523013246523
1111	25	0.3271940672627238
1112	1	-0.06276106789902514
1112	2	-0.23131121064041693
1112	3	-0.10905336417699729
1112	4	-0.2647471641817063
1112	5	-0.045776370641235076
1112	6	-0.025620155396488542
1112	7	-0.19852043186617863
1112	8	0.021388358932966867
1112	9	-0.12874911293856428
1112	10	0.047087508982841086
1112	11	-0.15381521258146336
1112	12	0.09481475049111054
1112	13	-0.034303272338348734
1112	14	0.01994667518642501
1112	15	-0.016921749168510303
1112	16	-0.31997492109854875
1112	17	-0.09544197909933416
1112	18	-0.14485652300734841
1112	19	-0.15846723292109743
1112	20	0.07867470001242793
1112	21	0.2532416118998668
1112	22	0.2202715209980397
1112	23	0.3150547939652881
1112	24	0.3120073737722535
1112	25	0.3658232027436412
1113	1	-0.2973311897878901
1113	2	-0.8245842751571395
1113	3	-0.34847813417466966
1113	4	-0.2751124986519642
1113	5	-0.16941834261565702
1113	6	-0.3456194631738754
1113	7	-0.1242979106131088
1113	8	0.007246390716822759
1113	9	-0.1340005654261347
1113	10	-0.3893723107281275
1113	11	-0.04288881070392847
1113	12	-0.20591070230725705
1113	13	-0.20666373223287593
1113	14	-0.16546060779959504
1113	15	-0.17293981198351815
1113	16	-0.485651237453375
1113	17	-0.300683188090602
1113	18	-0.06208889811929197
1113	19	-0.38168472909245565
1113	20	-0.188755893745018
1113	21	-0.40798586311500173
1113	22	-0.4732523343742798
1113	23	-0.24217200141283257
1113	24	-0.5507593176640952
1113	25	-0.49207503109230544
1114	1	-0.6705654606634184
1114	2	-0.5656572791577368
1114	3	-0.520396442788627
1114	4	-0.22883964039672833
1114	5	-0.47492263452068784
1114	6	-0.8029334575031228
1114	7	-0.5838152863087771
1114	8	-0.8723485185529896
1114	9	-0.6729456501267563
1114	10	-0.777345913801355
1114	11	-0.7235057870794961
1114	12	-0.809782387201345
1114	13	-0.7315453141249364
1114	14	-1.8580804916841913
1114	15	-1.2082494006597742
1114	16	-1.1316633076848817
1114	17	-1.1913456821488921
1114	18	-1.1949261793394044
1114	19	-1.1505520027848632
1114	20	-0.42642237730520094
1114	21	-0.6329678761396096
1114	22	-0.8322394410883689
1114	23	-0.6350892946181048
1114	24	-1.4608899151276091
1114	25	-1.4029343553738869
1115	1	-2.0383885043209666
1115	2	-1.8909139483267765
1115	3	-1.6312193701388296
1115	4	-3.1095114739291922
1115	5	-2.0944125739351342
1115	6	-1.9444171163208068
1115	7	-1.6413409003587027
1115	8	-1.688361925618132
1115	9	-2.2384478335328737
1115	10	-2.0956167407465927
1115	11	-1.9742697148843504
1115	12	-2.377375988182557
1115	13	-2.013305150025843
1115	14	-2.918749576133898
1115	15	-2.9218689910464377
1115	16	-2.464690365003257
1115	17	-2.5038592953952374
1115	18	-2.3593192135866374
1115	19	-2.0576932739184386
1115	20	-2.2914455859077165
1115	21	-2.425213348083105
1115	22	-2.786029474606032
1115	23	-3.018095733815791
1115	24	-2.4166592882310027
1115	25	-2.385720867950937
1116	1	-2.444085698452856
1116	2	-2.2804440592925808
1116	3	-2.2281299371391263
1116	4	-2.328824962036297
1116	5	-2.449648165860655
1116	6	-2.8836927957759464
1116	7	-2.398640430340187
1116	8	-2.723055248247647
1116	9	-2.853115518788794
1116	10	-2.860309674931056
1116	11	-2.9067968089269023
1116	12	-3.51345864929014
1116	13	-2.978295892085381
1116	14	-3.6678192502285007
1116	15	-3.3723461310549823
1116	16	-3.7577410829792015
1116	17	-3.6851310645035014
1116	18	-4.405267084260327
1116	19	-3.3384173354999263
1116	20	-3.323893696122525
1116	21	-3.234696586679007
1116	22	-4.742425222427503
1116	23	-5.095013033863701
1116	24	-4.0482191540879775
1116	25	-4.477968799481026
1201	1	0.7260643247466056
1201	2	0.8106690619179808
1201	3	0.7098542247353272
1201	4	0.7090399998993959
1201	5	0.8628337705837481
1201	6	0.872231972346583
1201	7	0.9696089553036416
1201	8	1.0200859122879535
1201	9	1.1889550294528135
1201	10	1.1046546041436542
1201	11	1.107602168734438
1201	12	0.8972705950009967
1201	13	0.9894895042817566
1201	14	1.1216580353873624
1201	15	1.1578252012816321
1201	16	1.1207768384113308
1201	17	1.2521313362738766
1201	18	1.10230281486497
1201	19	0.8448731139760142
1201	20	1.0718594740803056
1201	21	1.0693869876319226
1201	22	1.2281547329232936
1201	23	1.1047076352182117
1201	24	0.9915761282190303
1201	25	0.9043874224277033
1202	1	1.1255911767669602
1202	2	1.154902581303002
1202	3	1.1115277758962172
1202	4	1.1220609591749309
1202	5	1.1864457161160544
1202	6	1.337710367831484
1202	7	1.211861551842368
1202	8	1.10673780450594
1202	9	1.4188459494821615
1202	10	1.3891612929470654
1202	11	1.4393340781366533
1202	12	1.5100962789424628
1202	13	1.466908113937997
1202	14	1.5542454169445605
1202	15	1.7351697725027009
1202	16	1.525879424438422
1202	17	1.6531642181472606
1202	18	1.6111845187295089
1202	19	1.8675818903142982
1202	20	1.8494034754297815
1202	21	1.7995839498124937
1202	22	1.6131722121430911
1202	23	1.7720502038284067
1202	24	1.7362756493374363
1202	25	1.6857227567099926
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2211	16	0.10080674274246348
2211	17	0.2560880374679684
2211	18	0.06435929989822142
2211	19	0.1160254303121917
2211	20	-0.09042774910680151
2211	21	-0.19191272623610445
2211	22	0.17884923810657227
2211	23	0.0201175286371722
2211	24	-0.04312205877883457
2211	25	-0.12461811278901536
2212	1	-0.06100620238036569
2212	2	-0.027292019891618224
2212	3	0.06730809570414564
2212	4	0.16474228541733282
2212	5	-0.11119602688553698
2212	6	0.02501022272939224
2212	7	-0.12505138623967582
2212	8	-0.22650482621022405
2212	9	-0.3472569689325482
2212	10	-0.24701758366180115
2212	11	-0.19730847555410236
2212	12	-0.0942121727075147
2212	13	-0.12293172357947668
2212	14	-0.49525716592570035
2212	15	-0.20840861477604022
2212	16	-0.002191599356592633
2212	17	-0.3275588794675386
2212	18	-0.040807270971228604
2212	19	-0.13674382908119043
2212	20	-0.3516277172113078
2212	21	-6.552465777858174E-4
2212	22	-0.050324668725373556
2212	23	-0.5022124700670716
2212	24	-0.25276678954435994
2212	25	-0.4107618484817337
2213	1	-0.6138604657430271
2213	2	-0.6742591827718876
2213	3	-0.6137486738677644
2213	4	-0.5497481651416862
2213	5	-0.5047464739719061
2213	6	-0.391884522384359
2213	7	-0.8926251508585992
2213	8	-0.5663944426472511
2213	9	-0.8988601179249613
2213	10	-0.6543805598727843
2213	11	-0.6041845260131993
2213	12	-0.8770024528596778
2213	13	-0.7177846198440463
2213	14	-0.7198605050216145
2213	15	-0.8026675233897933
2213	16	-1.1504975506942188
2213	17	-0.9318203548216815
2213	18	-0.9152701599542112
2213	19	-1.2817641468216934
2213	20	-0.9280467506208012
2213	21	-1.5717836378149102
2213	22	-1.0269963270386953
2213	23	-1.0074727437200295
2213	24	-1.1198031171565148
2213	25	-1.3876536356673839
2214	1	-1.2583108479721226
2214	2	-1.1541875124477983
2214	3	-1.0775525071499317
2214	4	-1.5966196474780432
2214	5	-1.6206128662371206
2214	6	-3.0130164662760883
2214	7	-1.6751072332775792
2214	8	-2.3210266726896585
2214	9	-1.7091685542535942
2214	10	-1.8632639278853063
2214	11	-1.576554687508306
2214	12	-1.7072311460038136
2214	13	-1.8166371745443115
2214	14	-1.9899818253322508
2214	15	-2.343191766606502
2214	16	-1.9386384050529912
2214	17	-1.9819039259822446
2214	18	-2.073640920281221
2214	19	-3.685431423690485
2214	20	-2.33767183637125
2214	21	-2.6206837579602933
2214	22	-2.4421100103475837
2214	23	-2.614415782678215
2214	24	-2.0200720229996705
2214	25	-1.6898948967012402
2215	1	-1.9056621484969085
2215	2	-1.8849839647534168
2215	3	-1.7522084748242364
2215	4	-1.6441386756740783
2215	5	-2.629469395544991
2215	6	-2.267649943194474
2215	7	-2.762537187914866
2215	8	-3.606841613159041
2215	9	-2.8692893944167466
2215	10	-2.452772689114802
2215	11	-3.12708074247724
2215	12	-3.8798260963843774
2215	13	-3.1234879604501025
2215	14	-3.1961610652209416
2215	15	-4.3823930271053655
2215	16	-3.8760048264814735
2215	17	-3.4799305845166586
2215	18	-4.22773372959265
2215	19	-4.88619890485289
2215	20	-4.416339290849709
2215	21	-3.482936772611051
2215	22	-4.4852299991657425
2215	23	-4.505510968131652
2215	24	-3.473467237678385
2215	25	-3.7354653613831275
2216	1	-2.2680352782847883
2216	2	-2.0713245023789035
2216	3	-2.536409157918346
2216	4	-2.1484678191791566
2216	5	-2.1432215042869345
2216	6	-2.621813498602531
2216	7	-4.03214883370153
2216	8	-3.0047110924034897
2216	9	-3.3009478335328737
2216	10	-3.2603956183993503
2216	11	-3.4612658692783143
2216	12	-3.017478893751562
2216	13	-4.063590009732437
2216	14	-3.907907103551647
2216	15	-3.996715878954145
2216	16	-3.8171757659432934
2216	17	-4.137527932341545
2216	18	-4.21283010947041
2216	19	-4.073262637256992
2216	20	-4.199065583059117
2216	21	-4.437781002263421
2216	22	-4.390314832817115
2216	23	-3.440840001014198
2216	24	-3.7863876032858386
2216	25	-3.3892655909248752
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39976.0
3	0.0
4	611.0
5	5743.0
6	6656.0
7	5410.0
8	4303.0
9	3761.0
10	3351.0
11	2866.0
12	2648.0
13	2469.0
14	2572.0
15	2411.0
16	2494.0
17	2595.0
18	2906.0
19	3519.0
20	4477.0
21	5774.0
22	7339.0
23	8776.0
24	10512.0
25	12280.0
26	13381.0
27	14970.0
28	16979.0
29	20489.0
30	25689.0
31	32634.0
32	41720.0
33	54186.0
34	69881.0
35	91736.0
36	125991.0
37	198415.0
38	651514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.954920355516435	20.05834150114349	13.772461477940245	27.214276665399822
2	13.342411525929144	21.339637436352167	40.20691084073435	25.11104019698434
3	16.722072917735375	25.560446998535674	30.665727758331922	27.051752325397025
4	11.334008155170817	18.17920717985557	38.01883592566654	32.46794873930708
5	12.225104311868321	38.5806252912366	36.519386774875024	12.674883622020053
6	30.84035815418179	38.246007303635615	18.288092886989674	12.625541655192924
7	26.708068325398642	32.104500647747805	23.234220810031296	17.953210216822253
8	24.499999666677557	37.51350789207552	20.944915799417217	17.0415766418297
9	25.960491253234295	15.070058107371331	20.515274680302813	38.45417595909156
10	14.348063575314447	29.491718949143888	35.2400681078334	20.92014936770827
11	33.20641582971069	22.631422908442843	25.47317576908343	18.688985492763038
12	22.724880063965884	25.344882729211086	32.37086886993603	19.559368336886994
13	28.501519394347625	22.344790919211928	26.159300219131243	22.994389467309205
14	20.321501932516174	22.713144774477595	28.12632212955887	28.83903116344736
15	22.574536994161498	31.49852188588306	23.60305390302745	22.323887216927986
16	21.465648671844598	28.440596830338343	28.35037395184204	21.74338054597502
17	21.20327398644236	29.136333465728704	28.357488824142518	21.30290372368642
18	21.089105217013586	27.819431157112668	30.298158842167815	20.793304783705928
19	22.894077092864677	27.413824003027376	28.176931035884152	21.515167868223795
20	23.102763414489523	27.7482739069632	28.43937737394008	20.7095853046072
21	22.196417650327177	27.186537741518137	27.911042180481882	22.706002427672807
22	22.5597605912203	28.570895604421224	27.57877752025108	21.29056628410739
23	21.429922879725417	28.35295983030342	28.316318372959405	21.900798917011763
24	22.49425728432172	27.52872690255771	28.73689568243715	21.240120130683422
25	22.127231481080507	27.62316243216864	28.29559649958527	21.95400958716559
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	183.0
1	183.0
2	316.5
3	450.0
4	450.0
5	450.0
6	1105.0
7	1760.0
8	1760.0
9	1760.0
10	2488.5
11	3217.0
12	3217.0
13	3217.0
14	5523.0
15	7829.0
16	7829.0
17	7829.0
18	14025.0
19	20221.0
20	20221.0
21	20221.0
22	31868.0
23	43515.0
24	43515.0
25	43515.0
26	61723.0
27	79931.0
28	79931.0
29	79931.0
30	100474.0
31	121017.0
32	121017.0
33	121017.0
34	136991.5
35	152966.0
36	152966.0
37	152966.0
38	163720.5
39	174475.0
40	174475.0
41	174475.0
42	181379.5
43	188284.0
44	188284.0
45	188284.0
46	194140.5
47	199997.0
48	199997.0
49	199997.0
50	189002.5
51	178008.0
52	178008.0
53	178008.0
54	154902.0
55	131796.0
56	131796.0
57	131796.0
58	111423.5
59	91051.0
60	91051.0
61	91051.0
62	73471.5
63	55892.0
64	55892.0
65	55892.0
66	42823.0
67	29754.0
68	29754.0
69	29754.0
70	21434.0
71	13114.0
72	13114.0
73	13114.0
74	9055.0
75	4996.0
76	4996.0
77	4996.0
78	3404.0
79	1812.0
80	1812.0
81	1812.0
82	1191.5
83	571.0
84	571.0
85	571.0
86	365.0
87	159.0
88	159.0
89	159.0
90	96.0
91	33.0
92	33.0
93	33.0
94	18.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08287620400337367
2	6.662074276798527E-5
3	0.0
4	0.026048710422282236
5	0.06428901677110578
6	0.08214337583292583
7	0.02997933424559337
8	0.06562143162646548
9	0.04716748587973357
10	0.10972436333887174
11	0.003930623823311131
12	0.015589253807708552
13	0.007128419476174424
14	0.009393524730285924
15	0.00899380027367801
16	0.020385947287003492
17	0.03184471504309696
18	0.001465656340895676
19	0.004330348279919042
20	0.006728695019566511
21	5.329659421438822E-4
22	1.998622283039558E-4
23	1.3324148553597054E-4
24	1.3324148553597054E-4
25	0.004596831250990983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1501034.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.13875178006126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.00823982829773	61.44257800281723
2	11.377657253660345	16.642952994281547
3	2.5932666937840594	5.690048670485172
4	0.8699920754834991	2.545205378376318
5	0.3748425276541855	1.370775729335511
6	0.20278451808731093	0.8898843919936306
7	0.12251535371075445	0.6272434031008093
8	0.08751635124239349	0.5120669352171258
9	0.056350500455540686	0.3709264738950017
>10	0.26863369385191593	3.425686359228436
>50	0.018674611972540556	0.932726259618458
>100	0.01569445813585271	2.2689239184350103
>500	0.0023722732205522638	1.1405291162519033
>1k	0.0014598604434167778	2.1404523669638875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3401	0.2265771461539179	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2865	0.1908684280302778	No Hit
CCCATGTACTCTGCGTTGATACCAC	2837	0.1890030472327742	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2699	0.17980938473079222	No Hit
GTCCTACAGTGGACATTTCTAAATT	2525	0.1682173754891628	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2406	0.16028950709977255	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2403	0.1600896448714686	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2304	0.15349419133743805	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2298	0.15309446688083014	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1597	0.10639332620047248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	475	0.0	12.217132	4
TCAATAC	160	3.6379788E-12	11.882549	3
TTACACC	150	2.0827429E-9	10.781786	4
GGCGAGG	560	0.0	10.507714	19
TACACCA	165	9.058567E-10	10.378189	5
AGGACCT	1115	0.0	10.153252	5
AAGGCGT	85	6.5241446E-4	10.070258	6
TGTAGGA	1655	0.0	10.051703	2
GTATAAG	190	9.822543E-11	10.006691	1
GACGTGG	715	0.0	9.970705	7
AGGACGT	1500	0.0	9.957296	5
GGACGTG	1470	0.0	9.898996	6
TGGCGAG	1470	0.0	9.878221	18
GTAGGAC	1585	0.0	9.715952	3
CTGTAGG	1635	0.0	9.651713	1
GTCCTAA	905	0.0	9.558878	1
TAATACC	80	0.004463321	9.513341	4
TAAGTCT	90	0.0010992644	9.51334	4
TAACTTA	90	0.0011019537	9.510799	6
GGTCTAG	90	0.0011066737	9.506356	1
>>END_MODULE