FastQCFastQC Report
Thu 26 May 2016
SRR1513017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869108
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT45210.24188008397588584No Hit
GTCCTAAAGTGTGTATTTCTCATTT42620.2280232067916889No Hit
GTACATGGGAAGCAGTGGTATCAAC41550.2222985509665573No Hit
CTGTAGGACGTGGAATATGGCAAGA41290.22090751310250667No Hit
CTTTAGGACGTGAAATATGGCGAGG38620.20662262426783257No Hit
CCCATGTACTCTGCGTTGATACCAC35080.18768310873421976No Hit
GTCCTACAGTGTGCATTTCTCATTT27870.14910855873496875No Hit
GTATCAACGCAGAGTACTTTTTTTT25770.1378732529099442No Hit
CTGTAGGACCTGGAATATGGCGAGA20390.10908946941535749No Hit
CTGAAGGACCTGGAATATGGCGAGA20240.10828694757071289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8350.012.7399484
CCGACCC550.003073478112.0884734
GGCGAGG9800.011.72346319
GTCCTAC22600.011.42569351
TTGCGAG855.3131174E-511.17870818
AGGACCT15850.011.1454225
TCCAACG1201.2720557E-711.08555118
GGACCTG15550.010.9296856
AGGACGT23400.010.8775675
GTAGGAC24200.010.8325283
TGTAGGA24800.010.7619452
TGGCGAG22650.010.61360418
GGACGTG23100.010.6045626
TAGAAAT6450.010.6025144
CTGTAGG25150.010.4187691
TCCTACA25500.010.3920242
TTAGGAC15300.010.3051133
GACGTGA13050.010.258737
CGTGCGC750.002658913810.1283110
TAGGACT3300.010.0737284
Produced by FastQC (version 0.11.5)