##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513017_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1869108
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.033023238892564	31.0	30.0	34.0	23.0	34.0
2	29.019294765203508	31.0	30.0	34.0	22.0	34.0
3	29.269401233101565	31.0	30.0	34.0	23.0	34.0
4	32.70948548719496	35.0	33.0	37.0	25.0	37.0
5	32.82952349462953	35.0	35.0	37.0	26.0	37.0
6	32.82971556485767	37.0	35.0	37.0	26.0	37.0
7	32.60354029836692	36.0	33.0	37.0	25.0	37.0
8	32.642070977172	36.0	33.0	37.0	25.0	37.0
9	33.94880392144274	38.0	34.0	39.0	24.0	39.0
10	33.814164831566714	38.0	34.0	39.0	23.0	39.0
11	33.91326825416188	38.0	34.0	39.0	24.0	39.0
12	33.81483038968321	38.0	34.0	39.0	23.0	39.0
13	33.82141374388211	38.0	34.0	39.0	23.0	39.0
14	34.794438844625354	39.0	34.0	41.0	22.0	41.0
15	34.75329515469411	39.0	34.0	41.0	22.0	41.0
16	34.64190191257006	39.0	34.0	41.0	19.0	41.0
17	34.59061916165358	39.0	34.0	41.0	19.0	41.0
18	34.27894428786352	39.0	34.0	41.0	17.0	41.0
19	34.51067782065028	39.0	34.0	41.0	18.0	41.0
20	34.59735392497384	39.0	34.0	41.0	18.0	41.0
21	34.53570312683911	39.0	34.0	41.0	18.0	41.0
22	34.44688535921948	39.0	34.0	41.0	18.0	41.0
23	34.172633149074315	39.0	34.0	41.0	11.0	41.0
24	34.18376626711779	39.0	34.0	41.0	10.0	41.0
25	34.094537073299136	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7215257580134882
1101	2	0.9417649532794279
1101	3	0.8906170473422534
1101	4	0.8300047009094058
1101	5	0.7102090554803198
1101	6	0.9143957322304956
1101	7	0.9648947783969888
1101	8	0.7210794107933722
1101	9	0.7636000222892108
1101	10	0.8675537000973321
1101	11	1.0087519448314453
1101	12	1.258456213883271
1101	13	0.9847792345548996
1101	14	1.0256608752508072
1101	15	0.863607491300904
1101	16	1.2866327459456173
1101	17	1.270844085374037
1101	18	1.5197301782989143
1101	19	1.03129957230896
1101	20	1.1603714059843426
1101	21	1.3324425599552328
1101	22	1.1271161292825198
1101	23	1.3401472442964675
1101	24	0.9523430010692735
1101	25	1.2935176446987384
1102	1	0.7813934679487318
1102	2	0.7921145173682582
1102	3	0.8752142446858926
1102	4	1.021470595012083
1102	5	0.8100846465666507
1102	6	0.7863440660534025
1102	7	1.0038073619362322
1102	8	0.9320218755105785
1102	9	1.0460951313677995
1102	10	1.1034257048279414
1102	11	1.0565932197924184
1102	12	1.1392653229964154
1102	13	1.17254309952515
1102	14	1.1917527746562158
1102	15	1.3977985837762787
1102	16	1.3811928798003947
1102	17	1.1303097702086333
1102	18	1.5035342208206472
1102	19	1.4126289717863685
1102	20	1.389637786971278
1102	21	1.154859395092302
1102	22	1.3857745960962475
1102	23	1.5709632170186651
1102	24	1.525773538465863
1102	25	1.4013375032795992
1103	1	0.7597524981821664
1103	2	0.8607112830872978
1103	3	0.7478349600262106
1103	4	0.9309765629444584
1103	5	0.5671017274861754
1103	6	1.0005543705752515
1103	7	0.7829335794907522
1103	8	0.7647194425120816
1103	9	1.0932580168242154
1103	10	1.0562166440091616
1103	11	1.2100101616261583
1103	12	1.358461050795917
1103	13	1.154654227764155
1103	14	1.65919028225008
1103	15	1.5210281492544553
1103	16	1.5778657583748128
1103	17	1.4849581250014552
1103	18	1.7356991620135247
1103	19	1.7957723059413695
1103	20	1.5909322829380486
1103	21	1.4175517312181611
1103	22	1.6450371289174868
1103	23	1.9234059986318215
1103	24	1.8223637211640522
1103	25	1.7072765643945544
1104	1	0.6021965045729836
1104	2	0.7206819548626697
1104	3	0.7828462216206624
1104	4	0.9900424932002352
1104	5	0.7587082105771401
1104	6	0.82391307177501
1104	7	1.0550843868490674
1104	8	0.8432012214741604
1104	9	0.9998170634535057
1104	10	1.1325659549992935
1104	11	1.1136940575460912
1104	12	1.061651812774997
1104	13	0.9668792756582931
1104	14	1.2148468757379547
1104	15	1.3874379473963856
1104	16	1.166528046457124
1104	17	1.2314695040197563
1104	18	1.453467124164213
1104	19	1.2137663090036384
1104	20	1.2156393011859024
1104	21	1.4482466261469256
1104	22	1.2804479783264853
1104	23	1.5757924534670948
1104	24	1.404940888050156
1104	25	1.1604444425918103
1105	1	0.6290958122345671
1105	2	0.7154237703278561
1105	3	0.7597437725474769
1105	4	0.8343450556290435
1105	5	0.5330765488539839
1105	6	0.46465780711829296
1105	7	0.5886498095009571
1105	8	0.7766282550333443
1105	9	0.5794778194781784
1105	10	0.9061656062412098
1105	11	0.7863966876554187
1105	12	0.8378758177234431
1105	13	0.7487338466821569
1105	14	0.9861722489054046
1105	15	1.0936719056341744
1105	16	1.0600025395929151
1105	17	1.0793779957057765
1105	18	1.1028308394548318
1105	19	1.0148944563326623
1105	20	0.9771180604133818
1105	21	1.0721961681014491
1105	22	0.6719170200369149
1105	23	0.9407588397342366
1105	24	1.1690802914260416
1105	25	1.1074126651090808
1106	1	0.643263353856117
1106	2	0.7523945670552337
1106	3	0.7688330790632527
1106	4	0.7593984423201832
1106	5	0.7764869071035392
1106	6	0.7779377978503064
1106	7	0.5298865928171281
1106	8	0.7930214583898874
1106	9	1.028642902712157
1106	10	0.9583451104952729
1106	11	0.9241551034139093
1106	12	0.8687634735163456
1106	13	0.8606578701914529
1106	14	1.024363573488273
1106	15	1.0771253381179164
1106	16	1.1134542252075619
1106	17	1.0347454904400877
1106	18	1.281268842021774
1106	19	0.8427458666443925
1106	20	0.9807570669511421
1106	21	0.8174484926232068
1106	22	0.8835913735357153
1106	23	1.283872321458702
1106	24	1.0998317552841002
1106	25	1.3567859815240908
1107	1	0.5158415427409224
1107	2	0.6400521838703028
1107	3	0.7910087672562405
1107	4	0.7872571671132818
1107	5	0.7734490212309737
1107	6	0.6461987919874232
1107	7	0.8723916802443554
1107	8	0.7465134310659352
1107	9	0.9471131553884504
1107	10	0.8475976924702522
1107	11	0.7728351752029141
1107	12	1.0942942165254124
1107	13	0.8947206090469422
1107	14	1.2009176523725387
1107	15	1.2199709294740515
1107	16	1.0566318673997088
1107	17	1.1882427574216834
1107	18	1.4770238989733713
1107	19	1.308185285107193
1107	20	1.2149729180129825
1107	21	1.2241940830005618
1107	22	1.1321971693321302
1107	23	1.24483338028422
1107	24	1.3101122289064406
1107	25	1.298603565558956
1108	1	0.6722869230136155
1108	2	0.7295437146356036
1108	3	0.6823470924760358
1108	4	0.6670576229684357
1108	5	0.6670299638634489
1108	6	0.739821995713136
1108	7	0.7952597136886155
1108	8	0.741094884836599
1108	9	0.9740047578728621
1108	10	0.9967113968060843
1108	11	0.9767669450946528
1108	12	1.0448572728231653
1108	13	0.9606490087751709
1108	14	1.1773691846873362
1108	15	1.1602488481403768
1108	16	1.0403152773162248
1108	17	1.167092842047829
1108	18	1.333142932124474
1108	19	1.1252143390069378
1108	20	1.169166467035467
1108	21	1.119785983971724
1108	22	1.040490925293902
1108	23	1.1713333290172017
1108	24	1.2440633489512578
1108	25	1.290584572360011
1109	1	0.41974059913049544
1109	2	0.4400624666823596
1109	3	0.6372548519747383
1109	4	0.7561782077741839
1109	5	0.5923563794790994
1109	6	0.6973638469147758
1109	7	0.7773370519652119
1109	8	0.7055367610638825
1109	9	1.0246003049537435
1109	10	1.1263170096736133
1109	11	0.698818082660182
1109	12	0.9146977351289607
1109	13	0.9595528429520215
1109	14	0.8042103476891214
1109	15	1.1071942442606115
1109	16	1.0943370797885024
1109	17	0.90098971433099
1109	18	-0.7163688006109084
1109	19	0.7084138368470434
1109	20	0.9833925651736308
1109	21	0.9648874380077004
1109	22	1.0680265974184735
1109	23	0.8256008925124263
1109	24	1.1492332657813051
1109	25	1.2109572665143915
1110	1	0.40038240915520973
1110	2	0.18830015513137255
1110	3	0.34464856132851907
1110	4	0.17224416079592686
1110	5	0.32996938564788536
1110	6	0.42361194336978514
1110	7	0.42927372524199825
1110	8	0.36131292037077145
1110	9	0.6067828575534904
1110	10	0.8691393442169115
1110	11	0.7466883422701045
1110	12	0.21526191446724852
1110	13	0.46002081047520704
1110	14	0.6887011406666375
1110	15	0.7075617396669145
1110	16	0.7191703566506504
1110	17	0.30068057618338173
1110	18	0.8422878996122876
1110	19	0.7374657665973245
1110	20	0.731047901434593
1110	21	0.7658537942714432
1110	22	0.7909215922961295
1110	23	0.6930607963050477
1110	24	1.0058640149975275
1110	25	0.9916948445727769
1111	1	0.33506819757658235
1111	2	0.5429281084799058
1111	3	0.5239305370091678
1111	4	0.524602047034719
1111	5	0.5272303198382176
1111	6	0.5572650976357991
1111	7	0.5030154681513324
1111	8	0.24987645322430296
1111	9	0.6614669719438169
1111	10	0.6015191548624017
1111	11	0.49641867476776724
1111	12	0.6307888224913967
1111	13	0.6410854942250879
1111	14	0.43136679211446705
1111	15	0.6296470693497795
1111	16	0.4431381612534224
1111	17	0.5191537788612877
1111	18	0.7670577540930879
1111	19	0.44390619606729587
1111	20	0.3544273651236409
1111	21	0.7312945031965938
1111	22	0.40725190022333635
1111	23	0.73116525314272
1111	24	0.8290836357214957
1111	25	0.24395377397591744
1112	1	-0.22891093618881087
1112	2	0.06984689913077347
1112	3	0.03716867308592953
1112	4	0.1690981651202179
1112	5	0.0984115351139323
1112	6	0.21758717858832455
1112	7	-0.19029074233082355
1112	8	0.10212536058245547
1112	9	0.354681378430989
1112	10	0.3916756748634711
1112	11	0.30825616077927975
1112	12	0.3689983424494656
1112	13	0.050147826813528695
1112	14	0.30992835134141217
1112	15	0.23119878928019943
1112	16	0.40293405424482387
1112	17	0.38094298061974285
1112	18	0.2257265496827756
1112	19	0.2624239915163713
1112	20	0.05679474611835644
1112	21	0.22720841196666441
1112	22	0.2092406768944315
1112	23	0.4957492474067138
1112	24	0.1874328604425628
1112	25	0.33388148123505346
1113	1	-0.8173937109666056
1113	2	-0.6938382658617215
1113	3	-0.8702429014072628
1113	4	-0.5468810448710144
1113	5	-0.8415768377526156
1113	6	-0.6351438962273619
1113	7	-0.6390662779165055
1113	8	-0.34309666849694764
1113	9	-0.6055249019564641
1113	10	-0.8560892835015608
1113	11	-0.8119851745478286
1113	12	-0.5125286877434831
1113	13	-0.686964999765749
1113	14	-0.7640745930421318
1113	15	-0.9624363674691807
1113	16	-0.93532586714187
1113	17	-1.011731346843142
1113	18	-0.9781294936789067
1113	19	-0.9902104797527471
1113	20	-0.951251692999719
1113	21	-0.8653418348929947
1113	22	-0.8485641087411366
1113	23	-1.294890620934801
1113	24	-0.5480232442363402
1113	25	-0.48519395919323927
1114	1	-1.6200217694627455
1114	2	-1.3798166387517412
1114	3	-1.1769698635404744
1114	4	-1.4016024636356939
1114	5	-1.4292474153392583
1114	6	-1.8918712687199175
1114	7	-1.9297983707139963
1114	8	-1.3894899029610386
1114	9	-1.5885743643271013
1114	10	-1.8646703664046989
1114	11	-2.5960254788703168
1114	12	-1.8957295140899575
1114	13	-1.7845259410597407
1114	14	-2.2450870316201375
1114	15	-2.0266280592166197
1114	16	-2.677999252758891
1114	17	-4.865769575919799
1114	18	-3.8103155013631813
1114	19	-2.6775899366949147
1114	20	-1.8403503211098027
1114	21	-2.9783237286662008
1114	22	-3.099865475472253
1114	23	-2.8816627460451585
1114	24	-2.5825596730079283
1114	25	-2.4890183910507275
1115	1	-1.3835537605383657
1115	2	-1.034863290807177
1115	3	-1.0805882958766873
1115	4	-1.0331740806774583
1115	5	-0.9397801024309942
1115	6	-1.1009996624066787
1115	7	-2.220482288654644
1115	8	-1.501843957917849
1115	9	-1.4341425954365619
1115	10	-1.2806487410063596
1115	11	-1.9284835007784018
1115	12	-1.6921784938704576
1115	13	-1.5661629875089034
1115	14	-1.565894994180745
1115	15	-3.0461792760041106
1115	16	-1.7093308038433364
1115	17	-1.7981981126292368
1115	18	-1.8720758017813495
1115	19	-1.9414619076807043
1115	20	-1.592533308393456
1115	21	-2.456978828960885
1115	22	-2.3971296772055055
1115	23	-1.8011489348326535
1115	24	-1.769491331815523
1115	25	-2.005263957019359
1116	1	-2.138859145772834
1116	2	-2.950365164359777
1116	3	-2.520645635726769
1116	4	-2.4163015300623094
1116	5	-2.660831442008096
1116	6	-2.502408585852727
1116	7	-2.1745308937639543
1116	8	-2.6756646056639504
1116	9	-2.8161558464212746
1116	10	-3.943478631545908
1116	11	-2.3851445766362893
1116	12	-2.374997693257722
1116	13	-2.8685550213619067
1116	14	-2.863409994863183
1116	15	-2.4912625225771095
1116	16	-2.358844190449126
1116	17	-2.6735567653656958
1116	18	-2.6006186269554945
1116	19	-2.8898492305899133
1116	20	-3.200005646027776
1116	21	-3.6536529293967774
1116	22	-2.8237933515385407
1116	23	-2.6633624987738465
1116	24	-3.3601247330460957
1116	25	-3.127392660558609
1201	1	0.4404362388725005
1201	2	0.45137337998344407
1201	3	0.7138546757269424
1201	4	0.8069129642147317
1201	5	0.7804850825578171
1201	6	0.7396437481035107
1201	7	0.5916483965109052
1201	8	0.13729089538720984
1201	9	0.48737744992693166
1201	10	0.7731909549992935
1201	11	0.29769919039633663
1201	12	-0.22504051814287607
1201	13	0.2004013167210985
1201	14	0.8231953056896444
1201	15	0.4641589618891402
1201	16	0.898887647906399
1201	17	0.9978719798651667
1201	18	1.3296974985603498
1201	19	0.9988357534480841
1201	20	0.8444662939395258
1201	21	0.78202530972181
1201	22	1.0495980387129578
1201	23	1.3495922723076745
1201	24	1.0618551392578865
1201	25	0.9474538024951968
1202	1	0.8068244167261227
1202	2	0.7622960667487071
1202	3	0.48942824417593656
1202	4	0.9076311098347531
1202	5	0.8385825891941465
1202	6	0.9596866472313081
1202	7	0.7881275868787441
1202	8	0.9338352639878877
1202	9	1.185804876927314
1202	10	1.413421330425912
1202	11	0.9284396151213983
1202	12	1.030970099983783
1202	13	1.1033426326103637
1202	14	1.3553897975364393
1202	15	0.9095336774750322
1202	16	1.176760230386492
1202	17	1.4069673059933478
1202	18	1.8410930237783987
1202	19	1.523610592280086
1202	20	1.327980941266027
1202	21	1.3067468209087636
1202	22	1.495423076634836
1202	23	1.4837041892587592
1202	24	1.6441408806306583
1202	25	1.4861068646870805
1203	1	0.7078032833777037
1203	2	0.8012582721380745
1203	3	1.0030672905974534
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2211	17	0.46588019230235744
2211	18	0.4205150587250728
2211	19	0.06283444059153709
2211	20	0.03318151268597802
2211	21	0.06908056775951366
2211	22	0.11081741326432137
2211	23	0.6588192217579731
2211	24	0.46773701994850114
2211	25	0.5030774801936317
2212	1	-0.16988880781284266
2212	2	-0.21040591506790918
2212	3	-0.17131572576698773
2212	4	-0.3101633663174823
2212	5	-0.3827321209902692
2212	6	-0.4404642117470985
2212	7	-0.37803239247135423
2212	8	-0.24907465083128244
2212	9	-0.10595179843907943
2212	10	-0.14258635592507574
2212	11	-0.5153657022273848
2212	12	-0.397776279113927
2212	13	-0.49059891670560773
2212	14	-0.6431486485587214
2212	15	-0.4236053938531583
2212	16	-0.37105632968463453
2212	17	-0.42998224515817185
2212	18	-3.175633967359353
2212	19	-1.4160813800719936
2212	20	-0.7140123475170626
2212	21	-0.7788739666554108
2212	22	-0.5413572600732195
2212	23	-0.6149788061236947
2212	24	-0.9941836095749821
2212	25	-0.6664683496990165
2213	1	-0.1536571346794382
2213	2	-0.11360047311281107
2213	3	-0.4775757788010182
2213	4	-0.6220740176580364
2213	5	-0.459268434164656
2213	6	-0.530816384483181
2213	7	-0.2656997975005666
2213	8	-0.8281879754129307
2213	9	-0.6490565886752293
2213	10	-0.5998390068988684
2213	11	-0.6896199221002988
2213	12	-0.6743111951239769
2213	13	-0.48822129229248645
2213	14	-0.5478313030545294
2213	15	-0.7943818388267161
2213	16	-1.276248017721123
2213	17	-0.8874654282478289
2213	18	-0.6152299540047892
2213	19	-0.5160299760673723
2213	20	-1.2500894011303316
2213	21	-0.9554319940922227
2213	22	-0.8202746905715728
2213	23	-1.3346845866000692
2213	24	-0.9570317290791053
2213	25	-1.1364064378542622
2214	1	-1.8293032441075887
2214	2	-3.778297059965176
2214	3	-2.0196873923526972
2214	4	-1.5974343382900855
2214	5	-1.6954201036978063
2214	6	-1.8000620161867893
2214	7	-1.8094976375289171
2214	8	-1.6801223994104824
2214	9	-1.985830241145642
2214	10	-2.0244969062723825
2214	11	-1.7112442906569711
2214	12	-2.2775292785796104
2214	13	-2.2252289016475615
2214	14	-3.4273606512788817
2214	15	-3.711232415760186
2214	16	-2.841406186390323
2214	17	-3.3364829494861468
2214	18	-2.7269900415809474
2214	19	-4.796800887528153
2214	20	-3.1563586097213623
2214	21	-3.2289473139133875
2214	22	-3.275126993400079
2214	23	-3.2760881226826903
2214	24	-3.529394057916168
2214	25	-3.4012917819620974
2215	1	-1.2711812744216502
2215	2	-2.876646416306187
2215	3	-1.5833420577103468
2215	4	-1.3272037744202692
2215	5	-1.2342165737134252
2215	6	-1.7166016289305936
2215	7	-2.2130301699198682
2215	8	-2.2139100761853
2215	9	-1.955002028102733
2215	10	-3.6014716934353963
2215	11	-2.6252732017511207
2215	12	-2.86183232974577
2215	13	-2.0947510662304047
2215	14	-3.609718087417388
2215	15	-3.2543350257562977
2215	16	-2.7919423868782935
2215	17	-3.163960188980539
2215	18	-1.8531760370839692
2215	19	-2.635094567210828
2215	20	-2.796400849517358
2215	21	-2.3808306586521084
2215	22	-1.9896463187697506
2215	23	-2.550873647687528
2215	24	-2.472861085603178
2215	25	-2.333399746943055
2216	1	-2.696811668281228
2216	2	-2.799820493684745
2216	3	-3.288143041168034
2216	4	-4.168553169727431
2216	5	-3.130069521703984
2216	6	-3.236968694818863
2216	7	-3.0925553706123843
2216	8	-3.6484206723449084
2216	9	-4.1367235774703275
2216	10	-3.645853946066154
2216	11	-4.357152407777182
2216	12	-4.745740670349875
2216	13	-5.148942290584841
2216	14	-4.630938065695439
2216	15	-4.51351893004389
2216	16	-3.6855111344375864
2216	17	-3.9013336898456394
2216	18	-3.7063755608003817
2216	19	-4.158688980189666
2216	20	-3.884086788252258
2216	21	-4.419872521328422
2216	22	-5.100473308319007
2216	23	-4.970703580051534
2216	24	-5.083141055567079
2216	25	-5.143960771325201
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89075.0
3	0.0
4	755.0
5	4391.0
6	5738.0
7	5007.0
8	4473.0
9	4130.0
10	3834.0
11	3672.0
12	3521.0
13	3533.0
14	3510.0
15	3518.0
16	3698.0
17	4031.0
18	4368.0
19	5304.0
20	6810.0
21	8808.0
22	11140.0
23	13387.0
24	15466.0
25	17936.0
26	19649.0
27	22007.0
28	25177.0
29	29559.0
30	35584.0
31	45286.0
32	57092.0
33	73012.0
34	93839.0
35	123119.0
36	169361.0
37	262966.0
38	686352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.216227171815056	18.79638453629557	12.251772430881235	25.73561586100813
2	14.387826537471792	20.388440969560992	41.789142749487254	23.434589743479954
3	18.596665496364928	25.214454225502873	29.84660127248906	26.34227900564314
4	12.109968107578986	17.155275254767403	38.69783228943054	32.03692434822306
5	12.658433130394059	38.381160099016526	36.2979059343012	12.662500836288219
6	31.22783641690412	37.45346550547819	18.63295474308036	12.68574333453733
7	27.326024418226343	31.60205012072125	23.078734224901112	17.993191236151304
8	24.398354726949105	37.72156654140529	21.104169587707762	16.775909143937845
9	26.650366617327713	15.038748738431835	20.27124652031941	38.039638123921044
10	14.824042754375855	28.968690350244902	35.31939759867846	20.887869296700792
11	33.81413806721313	22.16416177300023	25.593367088661783	18.428333071124854
12	23.460654267422125	24.701024660783126	32.3560669465023	19.482254125292446
13	29.12529920228777	22.283071986746002	25.739343368371596	22.852285442594635
14	20.520978442769596	22.776617942898305	27.741668588823167	28.96073502550893
15	22.80883338292621	31.75026812262784	23.19897588930014	22.241922605145813
16	21.479313594382376	27.99676350985797	28.843773529170953	21.680149366588697
17	21.51206504329566	28.918858028440653	28.26411182754729	21.304965100716394
18	21.46798218459383	27.346894836402896	30.608071775777816	20.577051203225455
19	23.491129263583314	26.979753999751686	27.55520663421484	21.973910102450155
20	23.544269792317195	27.700012896893885	28.194590047343908	20.561127263445012
21	22.490124901730667	27.016160322039784	27.200095901238846	23.293618874990702
22	23.014894080415086	28.7495404630634	27.016223326121658	21.21934213039986
23	21.742871775167593	28.446167125907667	27.996808413288004	21.81415268563674
24	23.050224909217583	27.318951558096998	28.36927706910594	21.261546463579474
25	22.474001381741004	27.571245017143326	28.020498143854116	21.934255457261553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	274.0
1	274.0
2	392.0
3	510.0
4	510.0
5	510.0
6	1038.0
7	1566.0
8	1566.0
9	1566.0
10	2255.5
11	2945.0
12	2945.0
13	2945.0
14	5357.5
15	7770.0
16	7770.0
17	7770.0
18	14472.5
19	21175.0
20	21175.0
21	21175.0
22	34841.5
23	48508.0
24	48508.0
25	48508.0
26	70819.5
27	93131.0
28	93131.0
29	93131.0
30	121762.5
31	150394.0
32	150394.0
33	150394.0
34	169283.0
35	188172.0
36	188172.0
37	188172.0
38	201935.5
39	215699.0
40	215699.0
41	215699.0
42	224484.0
43	233269.0
44	233269.0
45	233269.0
46	243512.0
47	253755.0
48	253755.0
49	253755.0
50	239204.0
51	224653.0
52	224653.0
53	224653.0
54	195846.5
55	167040.0
56	167040.0
57	167040.0
58	142405.0
59	117770.0
60	117770.0
61	117770.0
62	95650.5
63	73531.0
64	73531.0
65	73531.0
66	56549.0
67	39567.0
68	39567.0
69	39567.0
70	28878.0
71	18189.0
72	18189.0
73	18189.0
74	12710.0
75	7231.0
76	7231.0
77	7231.0
78	4973.5
79	2716.0
80	2716.0
81	2716.0
82	1785.5
83	855.0
84	855.0
85	855.0
86	566.5
87	278.0
88	278.0
89	278.0
90	178.5
91	79.0
92	79.0
93	79.0
94	48.5
95	18.0
96	18.0
97	18.0
98	15.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3505950431970758
2	0.03830704271770277
3	0.03445493786340864
4	0.03418743058186044
5	0.039216567474966664
6	0.017815984951110368
7	0.018190495145277857
8	0.025787701941246844
9	0.02150758543647558
10	0.02070506359183097
11	0.019581533009328515
12	0.02097257087337917
13	0.020812066504450253
14	0.02926529660137349
15	0.029960815533398816
16	0.022310107281120192
17	0.02380814805779013
18	0.10486285436689587
19	0.026322716504343247
20	0.02359414223255157
21	0.027767255824703546
22	0.020277051941353843
23	0.02380814805779013
24	0.018939515533612825
25	0.024129156795647977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1869108.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.30619123386695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1187551761905	59.98188042948276
2	11.036139120699122	15.738900932482638
3	2.6442968995174683	5.656642211883422
4	0.9379881486102538	2.675374491996143
5	0.3973277160031046	1.416596304991648
6	0.21599414400876038	0.9241031842850441
7	0.14784619250065842	0.737964421295574
8	0.08983893608251764	0.5124857885237721
9	0.05769882273514421	0.3702854959129065
>10	0.3098298397887055	3.8991823980578917
>50	0.02176939807272705	1.0909444667159012
>100	0.018073604346510105	2.797141426440165
>500	0.0027856619228889706	1.4729049359060231
>1k	0.0016563395217177649	2.7255935120261245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4521	0.24188008397588584	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4262	0.2280232067916889	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4155	0.2222985509665573	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4129	0.22090751310250667	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3862	0.20662262426783257	No Hit
CCCATGTACTCTGCGTTGATACCAC	3508	0.18768310873421976	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2787	0.14910855873496875	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2577	0.1378732529099442	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2039	0.10908946941535749	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2024	0.10828694757071289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.350145630964075E-5	0.0
11	0.0	0.0	0.0	5.350145630964075E-5	0.0
12	0.0	0.0	0.0	1.070029126192815E-4	0.0
13	0.0	0.0	0.0	1.070029126192815E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	835	0.0	12.739948	4
CCGACCC	55	0.0030734781	12.088473	4
GGCGAGG	980	0.0	11.723463	19
GTCCTAC	2260	0.0	11.4256935	1
TTGCGAG	85	5.3131174E-5	11.178708	18
AGGACCT	1585	0.0	11.145422	5
TCCAACG	120	1.2720557E-7	11.085551	18
GGACCTG	1555	0.0	10.929685	6
AGGACGT	2340	0.0	10.877567	5
GTAGGAC	2420	0.0	10.832528	3
TGTAGGA	2480	0.0	10.761945	2
TGGCGAG	2265	0.0	10.613604	18
GGACGTG	2310	0.0	10.604562	6
TAGAAAT	645	0.0	10.602514	4
CTGTAGG	2515	0.0	10.418769	1
TCCTACA	2550	0.0	10.392024	2
TTAGGAC	1530	0.0	10.305113	3
GACGTGA	1305	0.0	10.25873	7
CGTGCGC	75	0.0026589138	10.12831	10
TAGGACT	330	0.0	10.073728	4
>>END_MODULE