FastQCFastQC Report
Thu 26 May 2016
SRR1513017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869108
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41730.22326157718013084No Hit
CTTTAGGACGTGAAATATGGCGAGG41630.22272656261703444No Hit
GTACATGGGAAGCAGTGGTATCAAC40850.21855344902488244No Hit
CCCATGTACTCTGCGTTGATACCAC40180.21496885145213654No Hit
CTGTAGGACGTGGAATATGGCAAGA39460.2111167465978424No Hit
GTCCTAAAGTGTGTATTTCTCATTT38490.20592710533580724No Hit
GTATCAACGCAGAGTACTTTTTTTT32780.17537777378300237No Hit
GTCCTACAGTGTGCATTTCTCATTT27480.14702200193889278No Hit
TATCAACGCAGAGTACTTTTTTTTT22270.11914774320156996No Hit
CTGAAGGACCTGGAATATGGCGAGA21470.11486762669679869No Hit
GGTATCAACGCAGAGTACTTTTTTT20860.11160403786191062No Hit
CTGTAGGACCTGGAATATGGCGAGA20620.11032000291047923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7900.012.8868874
CCGTGTT550.00306246712.0942679
TAGGGCG657.9219026E-411.7102994
TAGAAAT6800.011.613434
GGCGAGG11250.011.55693219
AGGACCT15500.011.1106185
TGGCGAG25850.010.86690118
ACGAAAT1605.529728E-1010.6787412
GGACCTG15700.010.6632086
CGAAATC1803.6379788E-1110.54690413
CTATACT2350.010.5268114
GGCGTGC1106.0126877E-610.368468
AGGACGT24150.010.3616755
CACCTTT7700.010.35514314
GACGTGA13750.010.3001637
ATAGGAC2400.010.2989393
GGACGTG23700.010.2746376
AAATGTC6850.010.2683747
TATACTG2600.010.2465115
GGCGAGA15250.010.20583519
Produced by FastQC (version 0.11.5)