##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513017_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1869108
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.38174894120618	31.0	31.0	34.0	26.0	34.0
2	30.503623653635852	31.0	31.0	34.0	27.0	34.0
3	30.61460867964826	31.0	31.0	34.0	27.0	34.0
4	34.095057107454465	37.0	35.0	37.0	30.0	37.0
5	33.74464557425253	37.0	35.0	37.0	28.0	37.0
6	33.61783214239092	37.0	35.0	37.0	28.0	37.0
7	33.48763581344684	37.0	35.0	37.0	28.0	37.0
8	33.35659469650764	37.0	35.0	37.0	27.0	37.0
9	34.792052679673944	39.0	35.0	39.0	27.0	39.0
10	34.660343329545434	39.0	35.0	39.0	27.0	39.0
11	34.70556650552028	39.0	35.0	39.0	27.0	39.0
12	34.55210078818345	39.0	35.0	39.0	27.0	39.0
13	34.53602895070804	39.0	35.0	39.0	27.0	39.0
14	35.564557532256025	40.0	36.0	41.0	27.0	41.0
15	35.581238751318814	40.0	36.0	41.0	27.0	41.0
16	35.55112224654755	40.0	36.0	41.0	27.0	41.0
17	35.529451481669334	40.0	36.0	41.0	27.0	41.0
18	35.45221784936986	40.0	36.0	41.0	26.0	41.0
19	35.427964569195574	40.0	36.0	41.0	26.0	41.0
20	35.41482300648224	40.0	36.0	41.0	26.0	41.0
21	35.35758286840568	40.0	36.0	41.0	25.0	41.0
22	35.319925868382136	40.0	36.0	41.0	25.0	41.0
23	35.20111571936988	40.0	35.0	41.0	25.0	41.0
24	35.19536698788941	40.0	36.0	41.0	25.0	41.0
25	35.09073579482833	40.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.44038093926827315
1101	2	0.47507069169740035
1101	3	0.45529035569948917
1101	4	0.28992861125081504
1101	5	0.5461771443304642
1101	6	0.5638990084130029
1101	7	0.6430869640324204
1101	8	0.5800449834959807
1101	9	0.35470805918493653
1101	10	0.6622973955716347
1101	11	0.6529286450107605
1101	12	0.7821972218832158
1101	13	0.925721064635951
1101	14	1.0656358996315376
1101	15	0.9418112892253632
1101	16	1.1117745702459274
1101	17	1.0606696243349916
1101	18	0.5215554537985696
1101	19	0.7196193844491958
1101	20	0.7023843580341236
1101	21	0.9330785036843849
1101	22	0.8922446566975921
1101	23	0.8581344228000631
1101	24	0.8958524655997593
1101	25	0.91840053896901
1102	1	0.5107780494488772
1102	2	0.4645204126412956
1102	3	0.4135051357856163
1102	4	0.4041556248801186
1102	5	0.6759497524827012
1102	6	0.48106748649648523
1102	7	0.5873491788566696
1102	8	0.6565391863861834
1102	9	0.8347980732577369
1102	10	0.8636507264859077
1102	11	0.7797007040742798
1102	12	0.9332405384559053
1102	13	0.9262259958750647
1102	14	1.0756742878449472
1102	15	0.9514961775741213
1102	16	0.9001090267152847
1102	17	0.9031111588651868
1102	18	0.9810484010802938
1102	19	1.025095824511169
1102	20	1.0339204337392474
1102	21	1.020720791579663
1102	22	1.0009380655210265
1102	23	0.8997811706013081
1102	24	0.8069982467360646
1102	25	0.794714944100491
1103	1	0.496960188865355
1103	2	0.38523206825891165
1103	3	0.44533453601196626
1103	4	0.4844269447795284
1103	5	0.6328763017069221
1103	6	0.6423857790994987
1103	7	0.6026334570086576
1103	8	0.6528502879927345
1103	9	0.779396733565882
1103	10	0.8739206634565377
1103	11	0.7824901157371968
1103	12	0.9745151323750818
1103	13	1.0006274477403991
1103	14	1.0545845073155107
1103	15	0.8577176313471
1103	16	0.9259744591160697
1103	17	1.0455190081170898
1103	18	1.0903710201748282
1103	19	1.12411049358424
1103	20	1.132868988088937
1103	21	0.7279859018256758
1103	22	0.9842821692187584
1103	23	1.0431248871722758
1103	24	1.111325367928437
1103	25	1.2196775315625743
1104	1	0.2757095000404064
1104	2	0.322265955672556
1104	3	0.22139870121160854
1104	4	0.19203497009870318
1104	5	0.3306240752767522
1104	6	0.37803267818525654
1104	7	0.42181367210695697
1104	8	0.3811302273761399
1104	9	0.5807634269842268
1104	10	0.6298219053755574
1104	11	0.6604290636564798
1104	12	0.535142013930475
1104	13	0.6845287996864897
1104	14	0.8354996875380039
1104	15	0.754436882941107
1104	16	0.7020383931663972
1104	17	0.6393616989909461
1104	18	0.6329599340689356
1104	19	0.653785442176634
1104	20	0.7824332254071606
1104	21	0.5570328408540348
1104	22	0.7002013991771889
1104	23	0.6589113911961206
1104	24	0.6410732441285631
1104	25	0.8285705852483858
1105	1	0.29110785814061657
1105	2	0.449879907660069
1105	3	0.35142504298713106
1105	4	0.2727903336873254
1105	5	0.2194703813258414
1105	6	0.3868566875717647
1105	7	0.4342088227793255
1105	8	0.5232143574041004
1105	9	0.6269639332936805
1105	10	0.5612292718267327
1105	11	0.5715340082176326
1105	12	0.5920721245492899
1105	13	0.5991303398805812
1105	14	0.8487321765907723
1105	15	0.7423722757839855
1105	16	0.8820819786805458
1105	17	0.6008099233943938
1105	18	0.8372236911513511
1105	19	0.9502418307737628
1105	20	0.9358632038085659
1105	21	0.6732053642278473
1105	22	0.8070109885450591
1105	23	0.9114474458657043
1105	24	0.9153403390070309
1105	25	1.0223314954958838
1106	1	0.5263642602105882
1106	2	0.45079838223742286
1106	3	0.4314592414843652
1106	4	0.47061402214439596
1106	5	0.5640173903484396
1106	6	0.5658778160218318
1106	7	0.6726733294096761
1106	8	0.5933681411772866
1106	9	0.6309422794145831
1106	10	0.6364405380833347
1106	11	0.41113299913847356
1106	12	0.6033254050599766
1106	13	0.5222930714547616
1106	14	0.7298957054305362
1106	15	0.6661872326322182
1106	16	0.7408504633377433
1106	17	0.5148407029541104
1106	18	0.7369648078887323
1106	19	0.5799641926137511
1106	20	0.7377128325874835
1106	21	0.8045367384528177
1106	22	0.7242271816110417
1106	23	0.7605777057432732
1106	24	0.7665531587456513
1106	25	0.8075563353358106
1107	1	0.5316239748163767
1107	2	0.5001643335351496
1107	3	0.5342980125292307
1107	4	0.5715596340548927
1107	5	0.6786472249614519
1107	6	0.689985569821502
1107	7	0.7350811795745997
1107	8	0.7938495893077686
1107	9	0.6074446832072624
1107	10	0.7312469800519565
1107	11	0.7815785616651141
1107	12	0.7938931548199548
1107	13	0.8126429093778285
1107	14	0.9698886893634295
1107	15	0.9388772247930817
1107	16	0.8215724953900931
1107	17	0.735409035532598
1107	18	0.8564679617821795
1107	19	0.9641281238892105
1107	20	0.9471050641061325
1107	21	0.9635550985705947
1107	22	1.110156697053064
1107	23	1.1660445433700346
1107	24	1.1995930956054934
1107	25	1.143673887604841
1108	1	0.4026620930749978
1108	2	0.4313256961719887
1108	3	0.44367893566164085
1108	4	0.4822254607522396
1108	5	0.4883281887244806
1108	6	0.48590574384703444
1108	7	0.48681782927663875
1108	8	0.495268837627151
1108	9	0.45028451924472535
1108	10	0.709930296407542
1108	11	0.6423195052124129
1108	12	0.7050552374930206
1108	13	0.7339689928873696
1108	14	0.7271425893499597
1108	15	0.8301211136496889
1108	16	0.7065104863730056
1108	17	0.6679160342738442
1108	18	0.6893743617771904
1108	19	0.6613427107728
1108	20	0.6816859203999215
1108	21	0.8333689363757557
1108	22	0.9080951610932146
1108	23	0.7700812984840653
1108	24	0.7896180331522018
1108	25	0.7324785935340543
1109	1	0.401807393076254
1109	2	0.40211133583220615
1109	3	0.39157750977813066
1109	4	0.49203765263990107
1109	5	0.4442881605898208
1109	6	0.23974435498902125
1109	7	0.3496464356104596
1109	8	0.6073793056824996
1109	9	0.6797251230472696
1109	10	0.6320683616698517
1109	11	0.7091626978624106
1109	12	0.645433319562251
1109	13	0.5401302437649065
1109	14	0.7986508572176803
1109	15	0.7926211372955407
1109	16	0.6202419801596051
1109	17	0.7609796335901322
1109	18	0.6878845065131429
1109	19	0.9278035862433143
1109	20	0.8362514215655779
1109	21	0.7960345365869088
1109	22	0.9784090076894216
1109	23	0.8050561209427016
1109	24	0.4920790595110347
1109	25	0.614949053751225
1110	1	0.13967623924230566
1110	2	0.21295460265823252
1110	3	0.1749524809046008
1110	4	0.27413087203678543
1110	5	0.23717958080801083
1110	6	0.2719505722337914
1110	7	0.2259506815115273
1110	8	0.25020614460736823
1110	9	0.45684743564557806
1110	10	0.42385132680882265
1110	11	0.36488216409097873
1110	12	0.4469681598442037
1110	13	0.10863367741281849
1110	14	0.5596779937867566
1110	15	0.582107644453842
1110	16	0.5776127792968069
1110	17	0.5816207069376631
1110	18	0.3609378537013299
1110	19	0.4879102313599546
1110	20	0.5775542484755078
1110	21	0.5853535162535124
1110	22	0.3601940652578577
1110	23	0.5328798633826111
1110	24	0.36566514412284334
1110	25	0.37377736425533215
1111	1	-0.18003129133238716
1111	2	0.05698620944453836
1111	3	0.0736398515553276
1111	4	0.11636627050342696
1111	5	0.4057113070715346
1111	6	0.17806983074603977
1111	7	0.4343013964172897
1111	8	0.5754406777079595
1111	9	-0.02674976008240293
1111	10	0.22810647074174284
1111	11	0.3831000770332622
1111	12	0.26457302641447455
1111	13	0.48208570494792724
1111	14	0.5994686319030933
1111	15	0.7150068097333602
1111	16	0.5285447690852152
1111	17	0.778329480885418
1111	18	0.7327057249953413
1111	19	0.8386777880981313
1111	20	0.8112132078491996
1111	21	0.6335203847489197
1111	22	0.5590716544371404
1111	23	0.5550425495004347
1111	24	0.6598659765482893
1111	25	0.70113683677355
1112	1	0.11997056798902861
1112	2	-0.15407071019442853
1112	3	0.05371466600085739
1112	4	-0.0997368474623812
1112	5	0.11226622852101542
1112	6	0.07447181138817882
1112	7	-0.03790988292367814
1112	8	0.19990202648295252
1112	9	-0.0787804295891803
1112	10	0.11767207515484301
1112	11	0.13467439933965863
1112	12	0.3320644183373531
1112	13	0.31807624100191134
1112	14	0.31279066985881343
1112	15	0.17300787651902283
1112	16	-0.08445723317153409
1112	17	0.07564717732562798
1112	18	-0.014261198838717348
1112	19	0.14680511442879407
1112	20	0.3341527020440509
1112	21	0.4660697816896402
1112	22	0.37830575346227135
1112	23	0.56117261600604
1112	24	0.5233095095378744
1112	25	0.4948209543337967
1113	1	-0.3860075718431588
1113	2	-0.7743938982271565
1113	3	-0.36038482408281425
1113	4	-0.4357633179910678
1113	5	-0.42727209908070307
1113	6	-0.47481884670178687
1113	7	-0.15161977726716458
1113	8	-0.026211011235815818
1113	9	-0.1499998035172112
1113	10	-0.4800481968390926
1113	11	-0.1633776219593983
1113	12	-0.2639817778256912
1113	13	-0.2136568671159651
1113	14	-0.16368173883781623
1113	15	-0.2020279159626739
1113	16	-0.5493869642145341
1113	17	-0.18167632597017302
1113	18	-0.11334462207505425
1113	19	-0.25336495409030846
1113	20	0.017756997720319134
1113	21	-0.27568406437521276
1113	22	-0.3979777866238621
1113	23	-0.21449023183180316
1113	24	-0.5748287029494321
1113	25	-0.5659663934939303
1114	1	-0.4893476624270505
1114	2	-0.3157807486722781
1114	3	-0.3317601492925988
1114	4	-0.08525886910953062
1114	5	-0.38120433820061095
1114	6	-0.6968136386554633
1114	7	-0.5889698642047207
1114	8	-0.9456942867475604
1114	9	-0.6127833764808628
1114	10	-0.8272711779004212
1114	11	-0.5590463376870645
1114	12	-0.685293731827926
1114	13	-0.5041477858636441
1114	14	-1.7455855951604633
1114	15	-0.8633469750438678
1114	16	-1.0083106971714315
1114	17	-1.0498013706700249
1114	18	-1.1149695278389586
1114	19	-0.9707264372931306
1114	20	-0.2970322358573938
1114	21	-0.3721908511325651
1114	22	-0.5636176951254512
1114	23	-0.38412152982186143
1114	24	-1.2102896127043792
1114	25	-1.0599630707556784
1115	1	-1.5723347514618915
1115	2	-1.4033189462362472
1115	3	-1.1414890241330369
1115	4	-2.443588814807626
1115	5	-1.6445106896578494
1115	6	-1.539437607251294
1115	7	-1.2519668156979193
1115	8	-1.3903575995945197
1115	9	-1.7999309438181754
1115	10	-1.5110958217364967
1115	11	-1.457294182190111
1115	12	-1.9677548798482505
1115	13	-1.3415156285566994
1115	14	-2.2548231959897436
1115	15	-2.3680556055707314
1115	16	-1.7523955941082576
1115	17	-1.684422102705767
1115	18	-1.5000845383594807
1115	19	-1.4775109523090535
1115	20	-1.4853623737444863
1115	21	-1.5921668542679157
1115	22	-1.8172931881328154
1115	23	-2.116740893179987
1115	24	-1.5672573276282407
1115	25	-1.363491539177204
1116	1	-1.2517593366038149
1116	2	-1.2302801942771744
1116	3	-1.0807549313711924
1116	4	-1.2886516996401838
1116	5	-1.4417139476354066
1116	6	-1.9149568997526494
1116	7	-1.3007762855421063
1116	8	-1.462410541110497
1116	9	-1.678487033848775
1116	10	-1.5361512901395074
1116	11	-1.5276319814823012
1116	12	-2.290815690359466
1116	13	-1.8279972411867291
1116	14	-2.245430538155908
1116	15	-2.012641550074342
1116	16	-2.2059273528498693
1116	17	-2.126393743408741
1116	18	-3.0842925967040458
1116	19	-2.4239525152286845
1116	20	-2.1297066773989712
1116	21	-2.2282653961740877
1116	22	-3.4702818185035618
1116	23	-3.3951662667820024
1116	24	-2.483216225646885
1116	25	-2.9439102578094065
1201	1	0.6238601642916137
1201	2	0.5948127551894622
1201	3	0.5499011166705436
1201	4	0.5847961415962857
1201	5	0.616070935180133
1201	6	0.5735716274606162
1201	7	0.602263551334012
1201	8	0.7053980413858056
1201	9	0.8331335960663537
1201	10	0.779821905375556
1201	11	0.8987065395018945
1201	12	0.6466229921913396
1201	13	0.7084795847106449
1201	14	0.7102204000983932
1201	15	0.6953185254531888
1201	16	0.6585299390601165
1201	17	0.6174565057542267
1201	18	0.700404076581016
1201	19	0.6199841136742208
1201	20	0.8192085877260027
1201	21	0.7447819350569276
1201	22	0.8241219909646276
1201	23	0.8578017897468442
1201	24	0.8074304301188988
1201	25	0.682088097325682
1202	1	0.6061245746063655
1202	2	0.7012286634185116
1202	3	0.6806522069246199
1202	4	0.5994588738678104
1202	5	0.7118165741638265
1202	6	0.6766041928758639
1202	7	0.5154662911899095
1202	8	0.4132253546205362
1202	9	0.6879283438858508
1202	10	0.6441350453073014
1202	11	0.6713044253570288
1202	12	0.5774250399729155
1202	13	0.6800369889578946
1202	14	0.5629033731738531
1202	15	0.9033508538566721
1202	16	0.6816338450138559
1202	17	0.788150002151653
1202	18	0.859214988602254
1202	19	0.8871639107921538
1202	20	0.9392953340286141
1202	21	0.8346461273208661
1202	22	0.8093336424625406
1202	23	0.7530307431029328
1202	24	0.5866018370206803
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2211	9	-0.17473811234726355
2211	10	-0.19716083858907751
2211	11	-0.023337526197607872
2211	12	0.08944006121548398
2211	13	0.03189648278008406
2211	14	-1.1552741406851652
2211	15	-0.45062082813025484
2211	16	-0.1919810360428329
2211	17	-0.022175941637058827
2211	18	-0.24456515629518094
2211	19	-0.2444902478430251
2211	20	-0.3680059368121391
2211	21	-0.19622842139779095
2211	22	-0.1957700289454536
2211	23	-0.19300221682688345
2211	24	-0.32477422438259396
2211	25	-0.37759126815846855
2212	1	-0.08872270218830991
2212	2	-0.09526544275638216
2212	3	-0.11026986839014796
2212	4	0.13983057203513027
2212	5	0.0055889090363479
2212	6	-0.005358694668231578
2212	7	-0.03918194353060045
2212	8	-0.155417201377972
2212	9	-0.3174067867534376
2212	10	-0.25445330470847694
2212	11	-0.13814978636178665
2212	12	-0.15291482293471148
2212	13	-0.14850862724080116
2212	14	-0.1556666213768665
2212	15	-0.1610458536317836
2212	16	0.05292302962968165
2212	17	-0.2588962019955403
2212	18	-0.033948747882107
2212	19	-0.04059069958442052
2212	20	-0.23631312095747603
2212	21	-0.1967394234864841
2212	22	-0.18029035730801013
2212	23	-0.597243728460441
2212	24	-0.18623436913413371
2212	25	-0.371991617893741
2213	1	-0.2161104414890893
2213	2	-0.2592184863915712
2213	3	-0.2010881118634984
2213	4	-0.2533144739985218
2213	5	-0.1294937128589666
2213	6	-0.11339617360161469
2213	7	-0.7092931636334683
2213	8	-0.36709563236241394
2213	9	-0.5652949437481354
2213	10	-0.3535410146105846
2213	11	-0.4411273182127644
2213	12	-0.5941892696721851
2213	13	-0.3197805998330949
2213	14	-0.33715384460006703
2213	15	-0.39499846065311317
2213	16	-0.6152153944271603
2213	17	-0.5532471754988961
2213	18	-0.721811290197266
2213	19	-1.103314413247233
2213	20	-0.5599256057851392
2213	21	-1.1677631665479637
2213	22	-0.8044703819330365
2213	23	-0.7910645363125042
2213	24	-0.9372401237047541
2213	25	-0.9199982427813111
2214	1	-0.9411968363506453
2214	2	-0.8447063520680835
2214	3	-0.7385040695850762
2214	4	-1.2869504062521386
2214	5	-1.1281045625719486
2214	6	-2.8189801548129836
2214	7	-1.290645488486156
2214	8	-1.7409069521915903
2214	9	-1.1118768406709592
2214	10	-1.5040292506938044
2214	11	-1.407433071930349
2214	12	-1.3347353893115468
2214	13	-1.532346121776186
2214	14	-1.6541903256562662
2214	15	-2.0778309607382113
2214	16	-1.471945333900706
2214	17	-1.5461473799233545
2214	18	-1.4983053807086435
2214	19	-3.196271104694439
2214	20	-1.8263959402123433
2214	21	-2.3186995196636886
2214	22	-1.9667237861708884
2214	23	-2.0777353008119235
2214	24	-1.3263043161881
2214	25	-1.1468236721027054
2215	1	-1.4272625916957509
2215	2	-1.2877036875757213
2215	3	-1.2025434327277225
2215	4	-1.1454171895380156
2215	5	-1.7645408456363043
2215	6	-1.5993641557947456
2215	7	-1.5212707185872247
2215	8	-2.4676234541561577
2215	9	-1.869453015824412
2215	10	-1.325051700237971
2215	11	-1.9328292097684283
2215	12	-2.4895141074848013
2215	13	-1.960861780088841
2215	14	-1.9218304975066616
2215	15	-3.0653220426968275
2215	16	-2.489437955066464
2215	17	-2.360286037526727
2215	18	-3.137126784839957
2215	19	-3.593276176797975
2215	20	-3.137425243388307
2215	21	-2.1488957217740605
2215	22	-3.0572143460470826
2215	23	-2.998622133726826
2215	24	-2.0443018076964634
2215	25	-2.246172343080538
2216	1	-1.4389918143994045
2216	2	-1.2700369403965333
2216	3	-1.646136744821085
2216	4	-1.255952526592452
2216	5	-0.9954111804971859
2216	6	-1.5413684410325317
2216	7	-2.801194401481819
2216	8	-1.9428468216278958
2216	9	-1.9003234054557154
2216	10	-1.8515803016259653
2216	11	-2.3643785061602074
2216	12	-1.7810406303352906
2216	13	-2.498850133706398
2216	14	-2.4577019743308313
2216	15	-2.576437562826875
2216	16	-2.2300164840753496
2216	17	-2.5410004957678396
2216	18	-2.2548137696899175
2216	19	-2.317282819354702
2216	20	-2.7663412600670014
2216	21	-2.75117030999634
2216	22	-2.9997507259303475
2216	23	-2.114810462916779
2216	24	-2.3972499351779035
2216	25	-2.1778671918676196
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48626.0
3	0.0
4	773.0
5	7095.0
6	8114.0
7	6429.0
8	5212.0
9	4622.0
10	4018.0
11	3719.0
12	3369.0
13	3084.0
14	3135.0
15	3091.0
16	3129.0
17	3351.0
18	3678.0
19	4300.0
20	5650.0
21	7226.0
22	9101.0
23	11111.0
24	13191.0
25	15231.0
26	17480.0
27	19216.0
28	21868.0
29	26342.0
30	33276.0
31	42638.0
32	53969.0
33	69355.0
34	90454.0
35	117189.0
36	161675.0
37	252100.0
38	786291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.33410683910098	19.59434316349581	13.327393722824008	27.7441562745792
2	13.363975417137702	20.904207196270733	40.48516216567591	25.24665522091566
3	16.882705547244996	25.381090873293573	30.41514990038029	27.321053679081142
4	11.343256104687878	17.790185404406806	37.94266613292346	32.92389235798185
5	12.27428917755222	38.470022476048925	36.53878483707274	12.716903509326114
6	31.163676510587447	37.95626026672606	18.24687688067146	12.63318634201503
7	26.975263050854714	31.749087483810197	23.146923134560012	18.128726330775077
8	24.447184378592038	37.90203373188489	20.790353813775965	16.8604280757471
9	26.247919108870082	14.803472880167433	20.19917674326487	38.74943126769761
10	14.413762518465036	29.26072364735234	35.329574803352045	20.995939030830588
11	33.69678506000993	22.15899849333128	25.510961871008615	18.63325457565018
12	23.250312491973666	24.803622338467335	32.34495261251851	19.601112557040487
13	28.922757539520116	22.164918391533373	25.889988416172415	23.0223356527741
14	20.310345547149762	22.67654173548877	27.728953545548862	29.284159171812608
15	22.672215545922185	31.809085879571914	23.18372832892626	22.33497024557964
16	21.45585463714403	27.993565780052847	28.75203744947179	21.798542133331335
17	21.356908031170576	29.034971906128632	28.254155479335125	21.353964583365666
18	21.368006548711392	27.34301016013311	30.55717549369473	20.731807797460768
19	23.32194423013336	27.055522680651755	27.651925750038252	21.970607339176624
20	23.41559723593287	27.680853511397896	28.174704451881638	20.728844800787595
21	22.353104828246675	26.938722008661898	27.427672602662774	23.280500560428656
22	22.89808378774001	28.58193016546966	27.10068567613145	21.419300370658885
23	21.456545047156183	28.32565052420727	28.048031467416546	22.169772961220005
24	22.938160876739065	27.313456472285175	28.507715980028976	21.240666670946784
25	22.29848847294088	27.502505300858143	28.052895689063405	22.146110537137574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	193.0
2	297.5
3	402.0
4	402.0
5	402.0
6	1049.5
7	1697.0
8	1697.0
9	1697.0
10	2397.5
11	3098.0
12	3098.0
13	3098.0
14	5511.5
15	7925.0
16	7925.0
17	7925.0
18	15073.0
19	22221.0
20	22221.0
21	22221.0
22	35780.0
23	49339.0
24	49339.0
25	49339.0
26	71908.0
27	94477.0
28	94477.0
29	94477.0
30	122673.0
31	150869.0
32	150869.0
33	150869.0
34	169534.5
35	188200.0
36	188200.0
37	188200.0
38	201614.0
39	215028.0
40	215028.0
41	215028.0
42	224216.0
43	233404.0
44	233404.0
45	233404.0
46	243152.5
47	252901.0
48	252901.0
49	252901.0
50	239553.5
51	226206.0
52	226206.0
53	226206.0
54	196589.0
55	166972.0
56	166972.0
57	166972.0
58	141595.0
59	116218.0
60	116218.0
61	116218.0
62	94564.5
63	72911.0
64	72911.0
65	72911.0
66	55964.5
67	39018.0
68	39018.0
69	39018.0
70	28221.5
71	17425.0
72	17425.0
73	17425.0
74	12205.5
75	6986.0
76	6986.0
77	6986.0
78	4794.5
79	2603.0
80	2603.0
81	2603.0
82	1660.0
83	717.0
84	717.0
85	717.0
86	476.5
87	236.0
88	236.0
89	236.0
90	144.5
91	53.0
92	53.0
93	53.0
94	30.0
95	7.0
96	7.0
97	7.0
98	4.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0760255694159995
2	5.350145630964074E-5
3	0.0
4	0.029104792232444568
5	0.07179895436753789
6	0.07618607378492842
7	0.0346689436886472
8	0.06746533640645698
9	0.04911433689225021
10	0.1105340087357178
11	0.004494122330009823
12	0.013910378640506595
13	0.006580679126085812
14	0.008506731553232878
15	0.0075972067959689865
16	0.018725509708374263
17	0.029479302426612054
18	0.0020330553397663485
19	0.003745101941674852
20	0.006580679126085812
21	1.6050436892892225E-4
22	2.1400582523856297E-4
23	0.0
24	0.0
25	0.0037986033979844932
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1869108.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.31944465764578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3127145029259	58.585038167667406
2	11.591912361724969	16.302736795931978
3	2.7900692548758985	5.885883616777342
4	0.9482012202581661	2.667079329290252
5	0.4424315168313815	1.5555769281311302
6	0.24462832264705975	1.0321276677643578
7	0.1433252576310315	0.70549867654197
8	0.09348972704902755	0.5259316549826002
9	0.06505269517422706	0.4117022458321285
>10	0.32356909046054283	4.009274549130555
>50	0.021139431830701404	1.0614581772350675
>100	0.018820076850040807	2.8310312449776953
>500	0.002589875724509746	1.3368042547708376
>1k	0.0020566660165224414	3.0898566909666894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4173	0.22326157718013084	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4163	0.22272656261703444	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4085	0.21855344902488244	No Hit
CCCATGTACTCTGCGTTGATACCAC	4018	0.21496885145213654	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3946	0.2111167465978424	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3849	0.20592710533580724	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3278	0.17537777378300237	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2748	0.14702200193889278	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2227	0.11914774320156996	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2147	0.11486762669679869	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2086	0.11160403786191062	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2062	0.11032000291047923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.350145630964075E-5	0.0
11	0.0	0.0	0.0	5.350145630964075E-5	0.0
12	0.0	0.0	0.0	5.350145630964075E-5	0.0
13	0.0	0.0	0.0	5.350145630964075E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	790	0.0	12.886887	4
CCGTGTT	55	0.003062467	12.094267	9
TAGGGCG	65	7.9219026E-4	11.710299	4
TAGAAAT	680	0.0	11.61343	4
GGCGAGG	1125	0.0	11.556932	19
AGGACCT	1550	0.0	11.110618	5
TGGCGAG	2585	0.0	10.866901	18
ACGAAAT	160	5.529728E-10	10.67874	12
GGACCTG	1570	0.0	10.663208	6
CGAAATC	180	3.6379788E-11	10.546904	13
CTATACT	235	0.0	10.526811	4
GGCGTGC	110	6.0126877E-6	10.36846	8
AGGACGT	2415	0.0	10.361675	5
CACCTTT	770	0.0	10.355143	14
GACGTGA	1375	0.0	10.300163	7
ATAGGAC	240	0.0	10.298939	3
GGACGTG	2370	0.0	10.274637	6
AAATGTC	685	0.0	10.268374	7
TATACTG	260	0.0	10.246511	5
GGCGAGA	1525	0.0	10.205835	19
>>END_MODULE