FastQCFastQC Report
Thu 26 May 2016
SRR1513016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109059
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC40910.19397276226032556No Hit
CCCATGTACTCTGCGTTGATACCAC33290.15784290529567924No Hit
GTATCAACGCAGAGTACTTTTTTTT31600.14982985302924196No Hit
GTCCTACAGTGGACATTTCTAAATT24390.11564399099313959No Hit
CTGTAGGACGTGGAATATGGCAAGA24340.11540691844087814No Hit
GTCCTAAAGTGTGTATTTCTCATTT23950.11355775253323877No Hit
CTTTAGGACGTGAAATATGGCGAGG21560.10222568453514103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4650.012.0501684
GTACCGG658.064846E-411.6854936
ACCGACC658.064846E-411.6854938
TAGACCG700.001495502710.8539035
GTCCTAC12850.010.6047971
GATATAC3650.010.4432731
TCCTACA13850.010.2171682
AATCCCG1606.2318577E-910.08810619
GTCCTAA7250.09.9895311
TGTAGGA14550.09.9867112
AGGGTCG1054.1195774E-59.9494125
GTAGGAC14200.09.8984553
GGCGAGG6700.09.77811119
TGGCGAG14350.09.60190518
GTATAGT1803.965397E-99.5294861
GTACCGT900.00111981489.4944646
AGGACCT11350.09.4553285
AGGACGT15050.09.3394065
CCTACAG14200.09.2965213
TCTATAC2151.07320375E-109.2763013
Produced by FastQC (version 0.11.5)