##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513016_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2109059
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.104053039767972	31.0	30.0	34.0	23.0	34.0
2	29.073493439491262	31.0	30.0	34.0	22.0	34.0
3	29.30693072123634	31.0	30.0	34.0	23.0	34.0
4	32.743631164419774	35.0	33.0	37.0	25.0	37.0
5	32.86105367369998	37.0	35.0	37.0	26.0	37.0
6	32.859085023226	37.0	35.0	37.0	26.0	37.0
7	32.64754708142352	36.0	33.0	37.0	25.0	37.0
8	32.65950549510469	37.0	33.0	37.0	25.0	37.0
9	33.981396442678935	38.0	34.0	39.0	24.0	39.0
10	33.857503275157306	38.0	34.0	39.0	23.0	39.0
11	33.959463438433914	39.0	34.0	39.0	25.0	39.0
12	33.85014169826449	38.0	34.0	39.0	23.0	39.0
13	33.84809528799337	39.0	34.0	39.0	23.0	39.0
14	34.85373145085083	39.0	34.0	41.0	22.0	41.0
15	34.790263809594705	39.0	34.0	41.0	22.0	41.0
16	34.67049143717649	39.0	34.0	41.0	19.0	41.0
17	34.61527866219011	39.0	34.0	41.0	18.0	41.0
18	34.30550164789131	39.0	34.0	41.0	17.0	41.0
19	34.53027772101207	39.0	34.0	41.0	18.0	41.0
20	34.61332091705353	39.0	34.0	41.0	18.0	41.0
21	34.55558000036984	39.0	34.0	41.0	18.0	41.0
22	34.471172688862666	39.0	34.0	41.0	18.0	41.0
23	34.19408940195604	39.0	34.0	41.0	11.0	41.0
24	34.19801579756659	39.0	34.0	41.0	10.0	41.0
25	34.1119390211464	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5189257678871968
1101	2	0.7617421119453844
1101	3	0.8155711110918098
1101	4	0.7283780357747744
1101	5	0.5957411549082536
1101	6	0.822740572379665
1101	7	0.9118780172000029
1101	8	0.7614861271361875
1101	9	0.6022661931848958
1101	10	0.7075198463654573
1101	11	0.9161168158960322
1101	12	1.1084951964523313
1101	13	0.9864578082652429
1101	14	0.9502487321823949
1101	15	0.7643853784731007
1101	16	1.255315026195099
1101	17	1.285962499037396
1101	18	1.4192483240895868
1101	19	0.7796993622137407
1101	20	1.0880389586892747
1101	21	1.0769572884264775
1101	22	1.1151101507945782
1101	23	1.3999940132109145
1101	24	1.091225755676163
1101	25	1.2430173364104817
1102	1	1.0067355597014682
1102	2	1.0655071988028304
1102	3	1.0445275158019918
1102	4	1.1914578543805803
1102	5	1.1302327178417784
1102	6	1.0449338929506524
1102	7	1.1029852169760375
1102	8	1.1941108063180792
1102	9	1.2040838105366802
1102	10	1.4009948636087017
1102	11	1.347594096265638
1102	12	1.3629035617306258
1102	13	1.2802654128077577
1102	14	1.3451960523248445
1102	15	1.5374080363249973
1102	16	1.504915909705872
1102	17	1.4709897949689292
1102	18	1.7358929577274296
1102	19	1.6663501815217856
1102	20	1.5533568794595212
1102	21	1.382005745449753
1102	22	1.7436280063454959
1102	23	1.9328091500385653
1102	24	1.878247891147815
1102	25	1.6449413261755836
1103	1	0.5771266597567468
1103	2	0.6246927525157489
1103	3	0.5933850798336842
1103	4	0.7310907086278604
1103	5	0.3549701418329363
1103	6	0.7655875690147766
1103	7	0.586141196338481
1103	8	0.5875878654667801
1103	9	0.9808665634388376
1103	10	0.8343604431185554
1103	11	0.8926972840305183
1103	12	0.990589207575475
1103	13	1.1210982854814304
1103	14	1.2043746961427928
1103	15	1.2796182813959334
1103	16	1.250652504509631
1103	17	1.1931037621699616
1103	18	1.5590756553504264
1103	19	1.6298728459772889
1103	20	1.3867892546207514
1103	21	1.2465879271339233
1103	22	1.2365430607033332
1103	23	1.558269809703205
1103	24	1.449733057906613
1103	25	1.399310683094697
1104	1	0.5066396553086427
1104	2	0.531359666934538
1104	3	0.4862259009985763
1104	4	0.7204333650642738
1104	5	0.43808793081672803
1104	6	0.3770851734422962
1104	7	0.58930794495393
1104	8	0.536113359160538
1104	9	0.7517817979076327
1104	10	0.8639129837213204
1104	11	0.7793514487437534
1104	12	0.794494257526118
1104	13	0.7339402310598118
1104	14	0.8437937745811155
1104	15	1.1496662881347959
1104	16	0.8267396598959564
1104	17	0.8954254854575225
1104	18	1.2120246679629147
1104	19	0.9840499468200292
1104	20	1.0444083948065241
1104	21	1.0680928116623747
1104	22	0.9027181299786378
1104	23	1.0957108732840197
1104	24	0.9991838105013358
1104	25	0.7996007436605623
1105	1	0.882414139847377
1105	2	0.9990003177025777
1105	3	0.9318139033674946
1105	4	1.076392471121224
1105	5	0.7300312720121767
1105	6	0.6648226005332774
1105	7	0.993185374576889
1105	8	0.9652400179220848
1105	9	0.8828485272318218
1105	10	1.1109558099370247
1105	11	1.0428741315826926
1105	12	1.0415872932848487
1105	13	1.109077138552145
1105	14	1.2328845405319058
1105	15	1.436401861867182
1105	16	1.2970878859843822
1105	17	1.2994502712067586
1105	18	1.328937916157571
1105	19	1.4347980316111375
1105	20	1.4377400438917505
1105	21	1.352896860739449
1105	22	1.1145124544863734
1105	23	1.4438423396326883
1105	24	1.4153292921380185
1105	25	1.4815489075901382
1106	1	0.6918927270504831
1106	2	0.7289416344618083
1106	3	0.7573467207047884
1106	4	0.7611448479202068
1106	5	0.8869751052168979
1106	6	0.8340900230093027
1106	7	0.5728354312055188
1106	8	0.8381922239383286
1106	9	0.983131661321643
1106	10	0.9940284244910984
1106	11	0.9681078526408129
1106	12	1.0440169523009573
1106	13	0.8813461592401168
1106	14	1.1152108638490361
1106	15	1.2919407868708035
1106	16	1.332773797205256
1106	17	1.2626621354799568
1106	18	1.4116360081052122
1106	19	1.0449186575681182
1106	20	1.1065246146692047
1106	21	1.0659133648460397
1106	22	1.0710246382026583
1106	23	1.4220223347878616
1106	24	1.3141926066966008
1106	25	1.3327330175839336
1107	1	0.5867190417226702
1107	2	0.6288508740214702
1107	3	0.8441922049274488
1107	4	0.9395410875711079
1107	5	0.8173854754544934
1107	6	0.6893484744877441
1107	7	0.9240738586226342
1107	8	0.7834329772651998
1107	9	0.9373350193236334
1107	10	0.8865039390039442
1107	11	0.8372777120091328
1107	12	1.0628148478620716
1107	13	1.0024208731593944
1107	14	1.2876078894278677
1107	15	1.2524902129903097
1107	16	1.2648470792100426
1107	17	1.3790282654420665
1107	18	1.5557571767401157
1107	19	1.4523383974311699
1107	20	1.346421729558024
1107	21	1.3258197679740675
1107	22	1.4458079551498102
1107	23	1.508903722075118
1107	24	1.5297181662315396
1107	25	1.4614207928053204
1108	1	0.5475518186434449
1108	2	0.5858823938335931
1108	3	0.4733854450904609
1108	4	0.55200165902599
1108	5	0.5062741085215166
1108	6	0.5982196093391252
1108	7	0.7033027859359677
1108	8	0.7496084659489242
1108	9	0.8612741900003087
1108	10	0.8003678213054215
1108	11	0.6077276003428906
1108	12	0.7042625949668135
1108	13	0.6600602257254167
1108	14	1.0173997527575978
1108	15	0.9790356968389915
1108	16	0.9697408235979523
1108	17	1.0328053473843681
1108	18	1.1601823833802314
1108	19	1.1194739855784306
1108	20	1.016086500584663
1108	21	1.0064681229115564
1108	22	0.8469729679281102
1108	23	1.061516942437514
1108	24	1.0169110840589966
1108	25	1.1855441552255996
1109	1	0.14298155574863003
1109	2	0.1369496441105369
1109	3	0.3318892232465025
1109	4	0.4757972873527052
1109	5	0.28074386075734026
1109	6	0.1720309987458819
1109	7	0.2562874841788343
1109	8	0.32203738347794086
1109	9	0.6135149652653169
1109	10	0.6476235921123035
1109	11	0.26886843234778723
1109	12	0.47668340679752674
1109	13	0.5802222496282212
1109	14	0.2800131199547309
1109	15	0.5548550478427501
1109	16	0.4684353558541261
1109	17	0.2795869348403528
1109	18	-1.1896559529702202
1109	19	0.20549435790017156
1109	20	0.450936049817777
1109	21	0.5508666350656028
1109	22	0.7636824146691694
1109	23	0.29747156853966317
1109	24	0.6819417506175895
1109	25	0.6461058801181991
1110	1	0.4227658473314193
1110	2	0.3815724817073054
1110	3	0.40239543427414404
1110	4	0.39419305828935336
1110	5	0.4772521012116684
1110	6	0.3867942595763054
1110	7	0.5542515109541739
1110	8	0.35114819999486
1110	9	0.6707112468319067
1110	10	0.8353062814299861
1110	11	0.697874504261506
1110	12	0.20306431709359174
1110	13	0.43278685577215015
1110	14	0.5584766475565743
1110	15	0.7123442869714012
1110	16	0.7717994579146463
1110	17	0.2490874340138518
1110	18	0.8803420830684701
1110	19	0.6841141840895943
1110	20	0.6496128308851894
1110	21	0.6643696873888061
1110	22	0.6705235465655406
1110	23	0.8135465918617015
1110	24	0.9236080081256475
1110	25	0.8284425030867268
1111	1	0.19558412821421456
1111	2	0.47176574125540327
1111	3	0.4563853511672882
1111	4	0.5176257784962672
1111	5	0.25702549388828544
1111	6	0.5161777196049258
1111	7	0.3671987190626709
1111	8	0.2738312517101704
1111	9	0.6506467832190523
1111	10	0.6170391841306326
1111	11	0.513142271142172
1111	12	0.5487258240454267
1111	13	0.5225567036065115
1111	14	0.49838500681977393
1111	15	0.6907667008776883
1111	16	0.5567982106553373
1111	17	0.5760334867663559
1111	18	0.8034386997134675
1111	19	0.5149562810607264
1111	20	0.37609871059687094
1111	21	0.5961741433868113
1111	22	0.48633616882977293
1111	23	0.8382521730189012
1111	24	0.8455952204354418
1111	25	0.27708167753235813
1112	1	-0.3305804820771421
1112	2	0.0519493927719914
1112	3	-0.00711530401738969
1112	4	0.09045263427461947
1112	5	0.10937634809870644
1112	6	0.13555792616157447
1112	7	-0.18717298117335446
1112	8	0.03919067327310444
1112	9	0.3345328003392254
1112	10	0.32135771759227794
1112	11	0.15761439791123877
1112	12	0.20550641328659225
1112	13	-0.1295421397300558
1112	14	0.1459141777050874
1112	15	0.09802210374451903
1112	16	0.3079265741758448
1112	17	0.396546943028099
1112	18	0.26418601712546774
1112	19	0.08814860164964955
1112	20	0.039441500241359506
1112	21	0.04772662409727957
1112	22	0.13280400229552214
1112	23	0.30377287500196104
1112	24	0.08341663238657304
1112	25	0.27069412644084423
1113	1	-0.3695521141137057
1113	2	-0.361567864217875
1113	3	-0.4588964860313389
1113	4	-0.17354763451964317
1113	5	-0.5241724480080237
1113	6	-0.17441022967544484
1113	7	-0.3152369153046095
1113	8	-0.08618903178708592
1113	9	-0.3669211202329521
1113	10	-0.524951555854166
1113	11	-0.5144661448763443
1113	12	-0.19813504168617158
1113	13	-0.41036760338911904
1113	14	-0.3034408707861971
1113	15	-0.716425322881939
1113	16	-0.7030732122918693
1113	17	-0.6129906556753184
1113	18	-0.7094923926381256
1113	19	-0.7340064121813157
1113	20	-0.8635968918030414
1113	21	-0.5673170129721044
1113	22	-0.47865193865461464
1113	23	-0.8807942667556219
1113	24	-0.30149233787054186
1113	25	-0.2712504674565537
1114	1	-1.4353592029218234
1114	2	-1.3273736755092749
1114	3	-1.0651440714146112
1114	4	-1.267710043898557
1114	5	-1.2898750888112893
1114	6	-1.7452984429978287
1114	7	-1.8307559681449064
1114	8	-1.419364951566081
1114	9	-1.6151195353192804
1114	10	-1.6666328295295258
1114	11	-2.506972936639798
1114	12	-2.0022494384625986
1114	13	-1.9388908072777014
1114	14	-2.1865018360866415
1114	15	-2.0451284363663973
1114	16	-2.7860677467229245
1114	17	-4.687734722821602
1114	18	-3.6324293242808494
1114	19	-2.600749964796723
1114	20	-1.7530792728651292
1114	21	-2.725432437929232
1114	22	-2.7825970424396367
1114	23	-2.7662553776048675
1114	24	-2.4136649136393977
1114	25	-2.187334535105027
1115	1	-1.2708551543927342
1115	2	-0.8386047266000958
1115	3	-0.8737232960072276
1115	4	-0.8907914529748595
1115	5	-0.7219316102185189
1115	6	-0.8149735310073964
1115	7	-1.9107779283750546
1115	8	-1.3721149511222634
1115	9	-1.1578892582511173
1115	10	-0.9330251560534677
1115	11	-1.666369767052501
1115	12	-1.2952927322485834
1115	13	-1.25120895189567
1115	14	-1.2904874314538617
1115	15	-2.6265342283992865
1115	16	-1.3875599594513375
1115	17	-1.5009100620334834
1115	18	-1.537386974944539
1115	19	-1.5832897177840763
1115	20	-1.2020848490238123
1115	21	-2.05623605453237
1115	22	-2.086542250156427
1115	23	-1.43878423036481
1115	24	-1.389817470665875
1115	25	-1.8243272262717554
1116	1	-1.4503901327235482
1116	2	-2.117730528897802
1116	3	-1.8079279916132087
1116	4	-1.4111996539788016
1116	5	-1.9326107012223623
1116	6	-1.7667870852576861
1116	7	-1.422220904298559
1116	8	-2.064083064306338
1116	9	-2.282247167923071
1116	10	-3.2448250262737233
1116	11	-1.8229271744163213
1116	12	-1.8488838655350648
1116	13	-2.246787698949568
1116	14	-2.151392628359325
1116	15	-1.6576442150514765
1116	16	-1.722033460512101
1116	17	-2.1350994597680497
1116	18	-1.8826414407462337
1116	19	-2.2796837909516086
1116	20	-2.6250420788650857
1116	21	-3.043443335646728
1116	22	-2.264191891917264
1116	23	-2.179084707456667
1116	24	-2.9144045121920286
1116	25	-2.6379495604915846
1201	1	0.511997512809419
1201	2	0.5179695374591979
1201	3	0.7633384990295262
1201	4	0.8732398613830767
1201	5	0.8764389087562421
1201	6	0.8390871334234333
1201	7	0.7912166203533033
1201	8	0.3042957367201069
1201	9	0.6542542868265571
1201	10	0.8139357219362608
1201	11	0.4963358452448361
1201	12	-0.11692670524346482
1201	13	0.38795645536990264
1201	14	0.8872638047894768
1201	15	0.582554206301559
1201	16	1.0166893296373658
1201	17	1.1807343414672076
1201	18	1.5065562891946982
1201	19	1.1435045005180342
1201	20	0.9804539513157522
1201	21	0.9564754582335766
1201	22	1.1471147841538425
1201	23	1.533009435957979
1201	24	1.2828776349905837
1201	25	1.1446106132431098
1202	1	0.48232344389120385
1202	2	0.6837040179004639
1202	3	0.37708353368616443
1202	4	0.6846753904338101
1202	5	0.6915636896331137
1202	6	0.7066705924742251
1202	7	0.7905100391348157
1202	8	0.8026141905330206
1202	9	1.1491485543643662
1202	10	1.2515383543221574
1202	11	0.9314328469396571
1202	12	1.0576988896657085
1202	13	0.9651523077771884
1202	14	1.1723413169443333
1202	15	0.8741981416778728
1202	16	1.0083783144460625
1202	17	1.1928148308274018
1202	18	1.5279637482329917
1202	19	1.396512789574551
1202	20	1.2236075794443977
1202	21	1.2129608295625047
1202	22	1.2320610712940052
1202	23	1.2693176193591356
1202	24	1.5027245254769355
1202	25	1.384213475560685
1203	1	0.554928599627651
1203	2	0.7078169686859397
1203	3	0.8335652024850724
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2211	19	0.2490255603354825
2211	20	0.12369099654532079
2211	21	0.13716800492861836
2211	22	5.165090375101045E-4
2211	23	0.5592676111576225
2211	24	0.41375301393569686
2211	25	0.42053510386249116
2212	1	-0.3725407019749589
2212	2	-0.2562169552259519
2212	3	-0.34573797977641973
2212	4	-0.5448285976310316
2212	5	-0.4155293120445336
2212	6	-0.5233509673768424
2212	7	-0.5282706462458364
2212	8	-0.46009359615570133
2212	9	-0.4553413603026044
2212	10	-0.5139622987475292
2212	11	-0.6140415370067842
2212	12	-0.509639998395464
2212	13	-0.5307999040396396
2212	14	-0.5841782446442849
2212	15	-0.6447197519009862
2212	16	-0.5124445067107075
2212	17	-0.5587422974732377
2212	18	-3.405927819974764
2212	19	-1.5536292888653307
2212	20	-0.9484273739828311
2212	21	-1.0036222472943024
2212	22	-0.744303760201575
2212	23	-0.7367191023545203
2212	24	-1.0142245100277236
2212	25	-0.7434126479217582
2213	1	-0.30951820078417924
2213	2	-0.18959925301737357
2213	3	-0.5856826155777597
2213	4	-0.719434047629349
2213	5	-0.509688358662153
2213	6	-0.554336109545126
2213	7	-0.3991041596613556
2213	8	-0.8363810719296119
2213	9	-0.7702615698010433
2213	10	-0.7499412787495032
2213	11	-0.7893942497782476
2213	12	-0.768651601772973
2213	13	-0.6174299493720312
2213	14	-0.6853751267751491
2213	15	-0.8431239687143943
2213	16	-1.3375783066559919
2213	17	-0.9210402732242002
2213	18	-0.7101537873695563
2213	19	-0.5644759185276769
2213	20	-1.2862438365459141
2213	21	-1.0114579028153798
2213	22	-0.904326087708121
2213	23	-1.3313885980787674
2213	24	-1.1098097180725333
2213	25	-1.2765727802224305
2214	1	-1.9473934554850167
2214	2	-3.821083162418244
2214	3	-2.082483283062171
2214	4	-1.7425882342950665
2214	5	-1.7538951433210137
2214	6	-1.872341550357639
2214	7	-2.068645791524105
2214	8	-1.7520907072014822
2214	9	-1.954846234645096
2214	10	-2.0065934833164683
2214	11	-1.7155374111457533
2214	12	-2.2499325622418276
2214	13	-2.137318644413167
2214	14	-3.159868538031713
2214	15	-3.445988010619292
2214	16	-2.6153563816023464
2214	17	-3.210838003713384
2214	18	-2.532716840050316
2214	19	-4.859035134167804
2214	20	-3.078969131068906
2214	21	-3.029788668143013
2214	22	-3.1839814860239635
2214	23	-3.2077118858317597
2214	24	-3.4689896792010053
2214	25	-3.1783085794042947
2215	1	-1.148890423872004
2215	2	-2.8974588506218772
2215	3	-1.7008970251214706
2215	4	-1.226832133691822
2215	5	-1.1844234906327138
2215	6	-1.646043478623561
2215	7	-2.1300885323266776
2215	8	-2.1428824407555744
2215	9	-1.7176192058308928
2215	10	-3.219044184541616
2215	11	-2.4031139632544445
2215	12	-2.596548834447102
2215	13	-1.8770020330078303
2215	14	-3.215254033163845
2215	15	-3.1303969678247228
2215	16	-2.725421974709789
2215	17	-2.964545325690306
2215	18	-1.9392514319921546
2215	19	-2.5275750759647053
2215	20	-2.7728728991769245
2215	21	-2.289896536667463
2215	22	-1.9101580995879246
2215	23	-2.6442072039254363
2215	24	-2.440336942205928
2215	25	-2.420657355439129
2216	1	-1.9948433647424615
2216	2	-2.0526816012853253
2216	3	-2.5928027067756325
2216	4	-3.3205606783672366
2216	5	-2.3756704958115975
2216	6	-2.514183402195883
2216	7	-2.3704487394019083
2216	8	-2.8235709137853817
2216	9	-3.2694892847769346
2216	10	-2.7489345524656734
2216	11	-3.2665834074161886
2216	12	-3.7929169689592364
2216	13	-4.342086759285976
2216	14	-3.7040113862381006
2216	15	-3.4978839264888926
2216	16	-2.606448939945899
2216	17	-2.838271944076041
2216	18	-2.840830671209595
2216	19	-3.1666379569537497
2216	20	-3.0941649211453566
2216	21	-3.371875532834075
2216	22	-3.97921449321894
2216	23	-4.006510824467835
2216	24	-3.871957978635269
2216	25	-3.94624158372865
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101507.0
3	6.0
4	922.0
5	5185.0
6	6395.0
7	5772.0
8	5116.0
9	4709.0
10	4367.0
11	4303.0
12	4028.0
13	4034.0
14	3930.0
15	4115.0
16	4282.0
17	4548.0
18	5028.0
19	5971.0
20	7635.0
21	9926.0
22	12397.0
23	14702.0
24	17199.0
25	19453.0
26	21748.0
27	24079.0
28	27487.0
29	32644.0
30	40010.0
31	50125.0
32	62521.0
33	79652.0
34	103436.0
35	136762.0
36	185410.0
37	287326.0
38	802329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42899593405992	17.85349768391301	12.14628819402314	25.57121818800393
2	15.334676304309571	19.86234152223088	40.13668136914737	24.66630080431218
3	18.946661955230287	24.48840204046302	29.241514078578223	27.323421925728475
4	12.75936468547074	16.42389653064235	37.06957924427983	33.74715953960708
5	13.669327163295867	37.70334964541732	35.02122373358416	13.606099457702658
6	32.75222527303164	35.99773323279889	17.826416848055466	13.423624646114012
7	28.50971246254862	31.28820420214214	21.898731656055244	18.303351679254
8	25.982558845400877	35.87349946124778	20.19614185118298	17.94779984216836
9	27.005956872075448	15.00884212083805	19.31992732815247	38.66527367893403
10	15.630341049420771	28.239628049555453	33.74424043993649	22.385790461087286
11	34.75753036116616	22.169472427759892	24.007337232423627	19.065659978650324
12	23.763701738628164	24.30057941625875	30.907100860540794	21.028617984572296
13	29.231584907700466	21.39032132928783	25.39676517704457	23.981328585967134
14	21.874401230832273	21.34805349036183	26.66754249008296	30.110002788722934
15	23.898477995170904	29.480300635200233	22.867730092308687	23.75349127732018
16	23.20703159456222	27.350812413985416	26.48732319289936	22.95483279855301
17	22.73304774275792	27.79884217729854	26.870127424994532	22.59798265494901
18	22.904938836497987	26.82422541736045	28.224777531170318	22.046058214971247
19	24.12949905053506	26.516393209381206	26.776666116527142	22.577441623556595
20	24.349433564258018	26.66905052773752	26.841580101525924	22.13993580647854
21	23.886151585730865	26.10870263212685	26.474693422836744	23.530452359305542
22	24.14452446813909	26.86025055473066	26.088725606196476	22.906499370933773
23	23.330810365792352	26.88738838714083	26.607870141153395	23.173931105913418
24	23.772091353529472	26.62386594122314	26.806444009945523	22.79759869530186
25	23.59678500935682	26.38358131519068	26.656128979020423	23.363504696432077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	321.0
1	321.0
2	463.0
3	605.0
4	605.0
5	605.0
6	1158.0
7	1711.0
8	1711.0
9	1711.0
10	2385.0
11	3059.0
12	3059.0
13	3059.0
14	5249.0
15	7439.0
16	7439.0
17	7439.0
18	13588.0
19	19737.0
20	19737.0
21	19737.0
22	31088.5
23	42440.0
24	42440.0
25	42440.0
26	61922.0
27	81404.0
28	81404.0
29	81404.0
30	106591.0
31	131778.0
32	131778.0
33	131778.0
34	153034.0
35	174290.0
36	174290.0
37	174290.0
38	194231.0
39	214172.0
40	214172.0
41	214172.0
42	232043.0
43	249914.0
44	249914.0
45	249914.0
46	267045.5
47	284177.0
48	284177.0
49	284177.0
50	279417.0
51	274657.0
52	274657.0
53	274657.0
54	250305.0
55	225953.0
56	225953.0
57	225953.0
58	197580.5
59	169208.0
60	169208.0
61	169208.0
62	141245.5
63	113283.0
64	113283.0
65	113283.0
66	88944.5
67	64606.0
68	64606.0
69	64606.0
70	47715.5
71	30825.0
72	30825.0
73	30825.0
74	21678.0
75	12531.0
76	12531.0
77	12531.0
78	8628.5
79	4726.0
80	4726.0
81	4726.0
82	3137.5
83	1549.0
84	1549.0
85	1549.0
86	1017.5
87	486.0
88	486.0
89	486.0
90	312.0
91	138.0
92	138.0
93	138.0
94	86.5
95	35.0
96	35.0
97	35.0
98	25.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3534277609113828
2	0.03783677934092882
3	0.03385396046293631
4	0.03252635417027215
5	0.03821609542454716
6	0.015599373938803989
7	0.015409715896994821
8	0.022901208548456918
9	0.019060633201821286
10	0.01853907358684608
11	0.017164052783729615
12	0.018254586524132328
13	0.018064928482323158
14	0.026884027426449426
15	0.027974561166852135
16	0.01920287673317816
17	0.021146871661722123
18	0.10654040498629958
19	0.023849498757502755
20	0.020625312046746915
21	0.025414177602428385
22	0.017922684950966283
23	0.020435654004937746
24	0.017638197888252532
25	0.020720141067651496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2109059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.23552929580478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72393792847856	55.082406313914255
2	12.367477425582358	16.878027363770585
3	3.502696442367622	7.170250371224611
4	1.3876459375120183	3.7874702008522325
5	0.6422593312400404	2.191245270616688
6	0.3680451156817582	1.5068251963968282
7	0.23204740737672438	1.1083714364849018
8	0.15753613562253246	0.8599649277935351
9	0.11071150969129975	0.6799012616631112
>10	0.47176337758786396	5.419592883530944
>50	0.02031332503544642	0.956079907788831
>100	0.012496535105264956	1.8266988346459698
>500	0.001883574440986378	0.9230121083763954
>1k	0.001185954277657925	1.6101539229411248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4091	0.19397276226032556	No Hit
CCCATGTACTCTGCGTTGATACCAC	3329	0.15784290529567924	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3160	0.14982985302924196	No Hit
GTCCTACAGTGGACATTTCTAAATT	2439	0.11564399099313959	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2434	0.11540691844087814	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2395	0.11355775253323877	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2156	0.10222568453514103	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.482902090458351E-5	0.0	0.0	0.0
6	0.0	9.482902090458351E-5	0.0	0.0	0.0
7	0.0	9.482902090458351E-5	0.0	0.0	0.0
8	0.0	9.482902090458351E-5	0.0	0.0	0.0
9	0.0	9.482902090458351E-5	0.0	4.7414510452291754E-5	0.0
10	0.0	9.482902090458351E-5	0.0	4.7414510452291754E-5	0.0
11	0.0	9.482902090458351E-5	0.0	4.7414510452291754E-5	0.0
12	0.0	9.482902090458351E-5	0.0	4.7414510452291754E-5	0.0
13	0.0	1.4224353135687526E-4	0.0	4.7414510452291754E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	465	0.0	12.050168	4
GTACCGG	65	8.064846E-4	11.685493	6
ACCGACC	65	8.064846E-4	11.685493	8
TAGACCG	70	0.0014955027	10.853903	5
GTCCTAC	1285	0.0	10.604797	1
GATATAC	365	0.0	10.443273	1
TCCTACA	1385	0.0	10.217168	2
AATCCCG	160	6.2318577E-9	10.088106	19
GTCCTAA	725	0.0	9.989531	1
TGTAGGA	1455	0.0	9.986711	2
AGGGTCG	105	4.1195774E-5	9.949412	5
GTAGGAC	1420	0.0	9.898455	3
GGCGAGG	670	0.0	9.778111	19
TGGCGAG	1435	0.0	9.601905	18
GTATAGT	180	3.965397E-9	9.529486	1
GTACCGT	90	0.0011198148	9.494464	6
AGGACCT	1135	0.0	9.455328	5
AGGACGT	1505	0.0	9.339406	5
CCTACAG	1420	0.0	9.296521	3
TCTATAC	215	1.07320375E-10	9.276301	3
>>END_MODULE