FastQCFastQC Report
Thu 26 May 2016
SRR1513016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109059
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC40470.19188652380042476No Hit
CCCATGTACTCTGCGTTGATACCAC39880.18908906768373954No Hit
GTATCAACGCAGAGTACTTTTTTTT36950.17519661612121804No Hit
TATCAACGCAGAGTACTTTTTTTTT25810.12237685147736502No Hit
CTGTAGGACGTGGAATATGGCAAGA24820.11768281494258814No Hit
GTCCTACAGTGGACATTTCTAAATT24460.11597589256630562No Hit
CTTTAGGACGTGAAATATGGCGAGG23460.11123444152107645No Hit
GGTATCAACGCAGAGTACTTTTTTT22870.10843698540439124No Hit
GTCCTAAAGTGTGTATTTCTCATTT22070.10464382456820791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG508.606854E-515.2225315
TAGGACC4600.012.2028444
GTGCGCC1001.9479667E-611.39144811
ATAGGAC1950.011.2126773
TGGATCG600.005812392611.0997625
ATAAGCG600.00584744711.0908013
AGGACGT13800.010.9618775
GGACGTG13550.010.8804246
GGATCGT803.7295953E-410.7005446
AATCCCG1252.2709719E-710.62974919
GTAGGAC14000.010.5249423
TAGGACG15200.010.5155654
GTCTAGA2001.8189894E-1210.4577851
TTAGTAC1553.623427E-910.4263743
GACGTGG7450.010.3338557
TTAGGAC9700.010.1924293
TGGCGAG12400.010.10316218
GGCGAGG6300.010.09344219
GTCCTAC13350.010.0411831
CGTGGAA7650.09.93637859
Produced by FastQC (version 0.11.5)