##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513016_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2109059
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.44603873101701	31.0	31.0	34.0	27.0	34.0
2	30.586990216964058	31.0	31.0	34.0	27.0	34.0
3	30.674787665968566	31.0	31.0	34.0	27.0	34.0
4	34.156779397826234	37.0	35.0	37.0	30.0	37.0
5	33.78830653860324	37.0	35.0	37.0	28.0	37.0
6	33.6382889241126	37.0	35.0	37.0	28.0	37.0
7	33.515768880813674	37.0	35.0	37.0	28.0	37.0
8	33.39981290234176	37.0	35.0	37.0	27.0	37.0
9	34.85878014792379	39.0	35.0	39.0	27.0	39.0
10	34.74723703793967	39.0	35.0	39.0	27.0	39.0
11	34.76770256308619	39.0	35.0	39.0	27.0	39.0
12	34.61711739690544	39.0	35.0	39.0	27.0	39.0
13	34.59310147321625	39.0	35.0	39.0	27.0	39.0
14	35.65673696183938	40.0	36.0	41.0	27.0	41.0
15	35.65362277679287	40.0	36.0	41.0	27.0	41.0
16	35.622993951330905	40.0	36.0	41.0	27.0	41.0
17	35.59755654061835	40.0	36.0	41.0	27.0	41.0
18	35.52158806368148	40.0	36.0	41.0	26.0	41.0
19	35.49526779478431	40.0	36.0	41.0	26.0	41.0
20	35.46908597625766	40.0	36.0	41.0	26.0	41.0
21	35.41428333678669	40.0	36.0	41.0	25.0	41.0
22	35.37173782241275	40.0	36.0	41.0	25.0	41.0
23	35.2588917616814	40.0	36.0	41.0	25.0	41.0
24	35.235450501858885	40.0	36.0	41.0	25.0	41.0
25	35.1332831371716	40.0	36.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.647848452592882
1101	2	0.6379196041728008
1101	3	0.6806324553811898
1101	4	0.49166439940718476
1101	5	0.7615624417099198
1101	6	0.7758650984810487
1101	7	0.7144164476076327
1101	8	0.738373256642987
1101	9	0.6823105195927823
1101	10	1.0353772116996183
1101	11	1.0337595698702557
1101	12	1.0725157053612833
1101	13	1.106980664423702
1101	14	1.0575548797301977
1101	15	0.9934878450888007
1101	16	1.2029287835829692
1101	17	1.147923420173548
1101	18	0.5716931040397029
1101	19	1.025875413594484
1101	20	0.860766221662395
1101	21	1.0888810523186905
1101	22	1.0872505958234555
1101	23	1.0553530521206085
1101	24	0.8968517990824836
1101	25	0.9666522826606112
1102	1	0.7430714104224094
1102	2	0.7525800140066856
1102	3	0.6999230856939462
1102	4	0.6372085858938021
1102	5	0.7841616431250387
1102	6	0.6570473474061842
1102	7	0.7369720521689231
1102	8	0.7215357802400248
1102	9	0.901133295058429
1102	10	0.9585183044127277
1102	11	0.9604540994230604
1102	12	0.9084123577485173
1102	13	0.983166772550156
1102	14	1.11796731378751
1102	15	1.1566738015060238
1102	16	1.1185212667815634
1102	17	1.1240556089623936
1102	18	1.2029172880551826
1102	19	1.141860835432169
1102	20	1.2370600511631196
1102	21	1.2549016839921379
1102	22	1.1808863740214335
1102	23	1.269808615391966
1102	24	1.2569347347655864
1102	25	1.1746560003187838
1103	1	0.46780422781691655
1103	2	0.4370212338334767
1103	3	0.4257407565986817
1103	4	0.47973438283501935
1103	5	0.5042878638731523
1103	6	0.6667649630317598
1103	7	0.6143352362485217
1103	8	0.6455706982426079
1103	9	0.7426571572290612
1103	10	0.8531239797397916
1103	11	0.7431839685957158
1103	12	0.7349440511334464
1103	13	0.8306055048147556
1103	14	0.9991148170121278
1103	15	0.8289513028880009
1103	16	0.8619868094386476
1103	17	1.0775197696404817
1103	18	1.0983245111987188
1103	19	1.0644515853861023
1103	20	1.0987423802583791
1103	21	0.6989200182467883
1103	22	0.9454501293190205
1103	23	1.0750854718504144
1103	24	1.0637087351341137
1103	25	1.0842930005030453
1104	1	0.4032557465374502
1104	2	0.4036883769465405
1104	3	0.3961376173886677
1104	4	0.2857372262671092
1104	5	0.37748784938918334
1104	6	0.6165404614899757
1104	7	0.5177442102116814
1104	8	0.2655318964481168
1104	9	0.45139775694166673
1104	10	0.5714391509365129
1104	11	0.5692200489283863
1104	12	0.5244993932410154
1104	13	0.5528788020906248
1104	14	0.7714754945570235
1104	15	0.7398800303187372
1104	16	0.6379981145587337
1104	17	0.4858847390145087
1104	18	0.6276979079565947
1104	19	0.6367211663800845
1104	20	0.6228474233481975
1104	21	0.49148764504823106
1104	22	0.5022084261565567
1104	23	0.4475284793325258
1104	24	0.5219702203509371
1104	25	0.7132629424528147
1105	1	0.4219220090344358
1105	2	0.528431789351643
1105	3	0.5192729219156256
1105	4	0.3883113021730935
1105	5	0.3891229058604253
1105	6	0.5689405991968997
1105	7	0.628028957225709
1105	8	0.6863435930837412
1105	9	0.7887293095348085
1105	10	0.8002420750206198
1105	11	0.7804602919612762
1105	12	0.7419323335810404
1105	13	0.8280376634017728
1105	14	1.0342672872695857
1105	15	0.8586650566175038
1105	16	1.0479231293644702
1105	17	0.6998588308903066
1105	18	0.9260935026816242
1105	19	1.0347626950016746
1105	20	1.1197216221693793
1105	21	0.9688032166076752
1105	22	1.0744363030558475
1105	23	1.069252680650166
1105	24	1.103940431599412
1105	25	1.1907479361574573
1106	1	0.4631589029627001
1106	2	0.48560266250514417
1106	3	0.4380382420390525
1106	4	0.39440664808061854
1106	5	0.4970011250453794
1106	6	0.5646852534488076
1106	7	0.6347983930936749
1106	8	0.6100950590776648
1106	9	0.6484565427148539
1106	10	0.6771884520294122
1106	11	0.6170460648129819
1106	12	0.7238508510433377
1106	13	0.5330731634196155
1106	14	0.7551267343038361
1106	15	0.7139919073572614
1106	16	0.8013934560892864
1106	17	0.6460223159598613
1106	18	0.8153557687001154
1106	19	0.8434342947880964
1106	20	0.9095852876787163
1106	21	0.7623259558425772
1106	22	0.9460386079162717
1106	23	0.9631145394063481
1106	24	0.8361221621217751
1106	25	0.9012044203484564
1107	1	0.5035197672613805
1107	2	0.5027133826319812
1107	3	0.4036938718410319
1107	4	0.4339504266749259
1107	5	0.56332106898288
1107	6	0.6195914251318939
1107	7	0.5474964654214247
1107	8	0.6047187318394123
1107	9	0.6478678578029218
1107	10	0.6945167393556417
1107	11	0.6885245001878673
1107	12	0.6992226917415252
1107	13	0.7241211595496111
1107	14	0.8688245771563885
1107	15	0.8384356001154885
1107	16	0.8232072986098515
1107	17	0.7991277564559951
1107	18	0.9328998952228744
1107	19	1.1127310056823845
1107	20	1.0190657291485294
1107	21	1.0871927317464767
1107	22	1.0399270915651115
1107	23	1.1212892956482676
1107	24	1.0732256425433704
1107	25	1.1290107977175978
1108	1	0.521991896748176
1108	2	0.353328122576265
1108	3	0.3875275927444939
1108	4	0.3678854530373741
1108	5	0.4083513455007335
1108	6	0.46197279413702574
1108	7	0.40639638023274216
1108	8	0.456338467984736
1108	9	0.47623338631810697
1108	10	0.5542489706083771
1108	11	0.4099529071595214
1108	12	0.5110489528793707
1108	13	0.504555391585157
1108	14	0.6483240360931433
1108	15	0.6003297819587914
1108	16	0.6155115373223481
1108	17	0.5869342630293346
1108	18	0.56883915799542
1108	19	0.46996513641247617
1108	20	0.6304959780376223
1108	21	0.6919034355891753
1108	22	0.8390544831797868
1108	23	0.6969771366907977
1108	24	0.611562851577979
1108	25	0.6222486583304985
1109	1	0.08614939947259259
1109	2	0.0855541274576943
1109	3	0.07986725752883572
1109	4	0.17381522166471797
1109	5	0.1784845491428939
1109	6	-0.04115825699192044
1109	7	0.17799581463513192
1109	8	0.3379772600555597
1109	9	0.44753200925003256
1109	10	0.23089330157304033
1109	11	0.3304324042459754
1109	12	0.3989809190515885
1109	13	0.4150973025485669
1109	14	0.4038543739206375
1109	15	0.4358259857900819
1109	16	0.22103467008468414
1109	17	0.3514204968517163
1109	18	0.2795058066494249
1109	19	0.4942376499895147
1109	20	0.4795854379279234
1109	21	0.5964255146107291
1109	22	0.5371379358470065
1109	23	0.5120929130815952
1109	24	0.11366222743920673
1109	25	0.2813968395018591
1110	1	0.21207941698457944
1110	2	0.21068710400472312
1110	3	0.2592333890160461
1110	4	0.2818650080839262
1110	5	0.18563991484885634
1110	6	0.24058017609428362
1110	7	0.17338541545998964
1110	8	0.3239259351999948
1110	9	0.4275536128244397
1110	10	0.4417540076171349
1110	11	0.2953544186156236
1110	12	0.4449400686563436
1110	13	0.023297659334872378
1110	14	0.47102839725904033
1110	15	0.5228581129238421
1110	16	0.6340696449623451
1110	17	0.4240052335990683
1110	18	0.22222932999601142
1110	19	0.45161805292891444
1110	20	0.552376390612821
1110	21	0.4923290186450373
1110	22	0.42044017312626636
1110	23	0.4254339385967256
1110	24	0.4727987391165911
1110	25	0.46833322352176054
1111	1	-0.30001899833964174
1111	2	-0.18210439362548314
1111	3	-0.12986228233769026
1111	4	-0.12135095158365061
1111	5	0.1829780008966253
1111	6	-0.011385898452438425
1111	7	0.15154524845853246
1111	8	0.3262924456310188
1111	9	-0.09983029359172235
1111	10	0.018135511417995076
1111	11	0.13692294566802587
1111	12	0.04925689877962469
1111	13	0.20546644634236344
1111	14	0.36622579245644005
1111	15	0.5298263537630561
1111	16	0.20599712011562588
1111	17	0.41055124433862744
1111	18	0.3617385662794419
1111	19	0.353242115843301
1111	20	0.30333809318742766
1111	21	0.2622424982359348
1111	22	0.14195670915893288
1111	23	-0.003163748065475147
1111	24	0.1347947478867937
1111	25	0.24533966988304456
1112	1	0.16306642197577048
1112	2	0.012974800709883993
1112	3	0.12345950579516796
1112	4	-0.008759635332708626
1112	5	0.13306943975628371
1112	6	0.06513938128617269
1112	7	0.1986429819591251
1112	8	0.3160340009780498
1112	9	0.061935021327414574
1112	10	0.25765727927954174
1112	11	0.13317665889584873
1112	12	0.2878330355259493
1112	13	0.26540812455241536
1112	14	0.32793826755778355
1112	15	0.2701069333771642
1112	16	0.03230079594325019
1112	17	0.17273237538319108
1112	18	0.18446188580431055
1112	19	0.25001483632919985
1112	20	0.48710168322891434
1112	21	0.49591147067312846
1112	22	0.3196973955280029
1112	23	0.44685249077849676
1112	24	0.4866831401649563
1112	25	0.39840466994299106
1113	1	-0.3078075528592521
1113	2	-0.6552104356765476
1113	3	-0.20410441794684075
1113	4	-0.2942332945997208
1113	5	-0.1848188629626506
1113	6	-0.2239794465586158
1113	7	0.004912254574428232
1113	8	0.07029874476361897
1113	9	-0.05358364771655033
1113	10	-0.42617912227257193
1113	11	-0.1707411501832965
1113	12	-0.1795341467276188
1113	13	-0.1412819348785277
1113	14	-0.1784570465800428
1113	15	0.026196914026627383
1113	16	-0.3337270569785389
1113	17	-0.2659473849831855
1113	18	-0.009269548933723115
1113	19	-0.1800199168243779
1113	20	0.11805016005402535
1113	21	-0.23772720351682608
1113	22	-0.3111666409926457
1113	23	-0.0874294328512164
1113	24	-0.24940235763274643
1113	25	-0.3960057355782567
1114	1	-0.5044321774785843
1114	2	-0.3052374617629994
1114	3	-0.36149685654564223
1114	4	-0.15102367040549325
1114	5	-0.3220394887784437
1114	6	-0.796305791288134
1114	7	-0.6838686376520613
1114	8	-1.0239296549616697
1114	9	-0.6798856024492892
1114	10	-0.9327751173017091
1114	11	-0.6741314592938821
1114	12	-0.5761075825735915
1114	13	-0.49312616241923024
1114	14	-1.6750444470053196
1114	15	-0.9386898781685389
1114	16	-0.821553688268267
1114	17	-1.0402744971037805
1114	18	-1.0220957286873755
1114	19	-0.9522385977311032
1114	20	-0.37076227361972514
1114	21	-0.4341455752645018
1114	22	-0.6125802352683181
1114	23	-0.5278216921571612
1114	24	-1.1648979746490937
1114	25	-1.1638761019920807
1115	1	-1.4117607163687858
1115	2	-1.2969571057835836
1115	3	-0.8992721804901045
1115	4	-2.0500024398159766
1115	5	-1.3859742045825314
1115	6	-1.265056579069224
1115	7	-1.1237386160468859
1115	8	-0.9368940019865661
1115	9	-1.297486629272015
1115	10	-1.1987574426007086
1115	11	-1.1981592708513205
1115	12	-1.5680425682204415
1115	13	-1.1078139525667723
1115	14	-1.846880229931024
1115	15	-1.853328581787089
1115	16	-1.2670735882844255
1115	17	-1.3336819010921275
1115	18	-1.227736330081683
1115	19	-1.0173200565152811
1115	20	-1.2373724271638125
1115	21	-1.1961860658812498
1115	22	-1.447966740644759
1115	23	-1.8471570800827593
1115	24	-1.3392538581977362
1115	25	-1.151444783188829
1116	1	-0.7300186418429355
1116	2	-0.7977430129137382
1116	3	-0.6635824145979683
1116	4	-0.7566815577171724
1116	5	-0.6933287823444658
1116	6	-1.3632615167514146
1116	7	-0.803580728565251
1116	8	-1.0143089643134573
1116	9	-1.0593300395878202
1116	10	-1.2186206359221003
1116	11	-1.0703865736852904
1116	12	-1.5865215563418928
1116	13	-1.1917733779047595
1116	14	-1.6639249165263905
1116	15	-1.3716112192073666
1116	16	-1.7604721364996152
1116	17	-1.5242521922896302
1116	18	-2.323410226533582
1116	19	-1.6416510688309103
1116	20	-1.462332165183561
1116	21	-1.4882632814669279
1116	22	-2.81859247401799
1116	23	-2.7001571439639775
1116	24	-1.910598423418783
1116	25	-2.2083046177028436
1201	1	0.7189746342903547
1201	2	0.7724561972987445
1201	3	0.6806201645083938
1201	4	0.7205041629987363
1201	5	0.7848685346053372
1201	6	0.9154448465601277
1201	7	0.8264511506371619
1201	8	0.9132458962208361
1201	9	1.0050269703564467
1201	10	0.8790448041454653
1201	11	1.005390336862689
1201	12	0.9475177288586352
1201	13	0.9109175608843927
1201	14	0.948436511030792
1201	15	1.1079805228263098
1201	16	1.0695704155071013
1201	17	1.0140260523588864
1201	18	1.0341797614479091
1201	19	0.841511422294424
1201	20	1.0676444938574647
1201	21	0.9938644116760287
1201	22	1.0976979376274372
1201	23	1.0866025462462758
1201	24	1.1946818305011533
1201	25	1.051858908220467
1202	1	0.48046404503870477
1202	2	0.5719328392266085
1202	3	0.6078217066511797
1202	4	0.6507707451619353
1202	5	0.658107083256283
1202	6	0.686879095176387
1202	7	0.5730677098950281
1202	8	0.5172552371464718
1202	9	0.7661807550260136
1202	10	0.6779356863394455
1202	11	0.7751076126368446
1202	12	0.7781476733412589
1202	13	0.8080281121524209
1202	14	0.7438197640018984
1202	15	1.0483346254183914
1202	16	0.7872215365411463
1202	17	0.9111702735882972
1202	18	0.8453655472198989
1202	19	1.0033892770435742
1202	20	1.0045464982644248
1202	21	0.9963866333625688
1202	22	0.8445038294217397
1202	23	0.8457857110313611
1202	24	0.7516878077488442
1202	25	0.8319221335763487
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2211	8	0.08774894955327994
2211	9	-0.24805398770583054
2211	10	-0.2771736695076257
2211	11	7.520389491730839E-4
2211	12	0.09639340002571828
2211	13	0.052540224922992707
2211	14	-1.0445474525633784
2211	15	-0.4207950187761256
2211	16	-0.182313883948801
2211	17	0.09053769624288321
2211	18	-0.17133133185895133
2211	19	-0.03026767725553725
2211	20	-0.19316950879962747
2211	21	-0.3076840744303624
2211	22	-0.06712386019560768
2211	23	-0.12321911127152418
2211	24	-0.10582026875561468
2211	25	-0.3633520189456263
2212	1	-0.28659371346663676
2212	2	-0.2737564115509592
2212	3	-0.2759290544098256
2212	4	-0.19862592564146553
2212	5	-0.296273980366081
2212	6	-0.3275073700698883
2212	7	-0.33162097465407214
2212	8	-0.6125036406181508
2212	9	-0.6906058421420767
2212	10	-0.6505615198795738
2212	11	-0.5934591982529938
2212	12	-0.49927135283173385
2212	13	-0.4228516162920499
2212	14	-0.4945162358459143
2212	15	-0.39752571031823436
2212	16	-0.3416391131774432
2212	17	-0.5644861549615072
2212	18	-0.44380553216009844
2212	19	-0.41126995832024704
2212	20	-0.6314987411132122
2212	21	-0.5983113060175995
2212	22	-0.6240447900865362
2212	23	-1.0438062303969673
2212	24	-0.739135239313967
2212	25	-0.8222712268163193
2213	1	-0.4581629554472535
2213	2	-0.6711505932454287
2213	3	-0.4872281400039391
2213	4	-0.5197386357928977
2213	5	-0.4519251204138257
2213	6	-0.3088231162861703
2213	7	-0.851753178690899
2213	8	-0.46612487637476363
2213	9	-0.790644560930474
2213	10	-0.5921847364060113
2213	11	-0.6284732598753706
2213	12	-0.8435804381099814
2213	13	-0.6044938280216314
2213	14	-0.6542900723069494
2213	15	-0.8061749167530863
2213	16	-0.8948635775459124
2213	17	-0.8073640051927242
2213	18	-1.0398546587592605
2213	19	-1.3040447746968198
2213	20	-0.7646655129657702
2213	21	-1.5808223505363372
2213	22	-0.9071395335389951
2213	23	-1.0257010135435607
2213	24	-1.174950503357401
2213	25	-1.2151344959831292
2214	1	-1.0451069573451264
2214	2	-0.9830474368365678
2214	3	-0.8821312047097045
2214	4	-1.3249875211996027
2214	5	-1.330296525264501
2214	6	-2.716993006121399
2214	7	-1.3783154978970558
2214	8	-1.9360854971592936
2214	9	-1.57479948247844
2214	10	-1.6881785067516972
2214	11	-1.4902731686414938
2214	12	-1.496727185864252
2214	13	-1.6813328940652212
2214	14	-1.6880538339881355
2214	15	-2.2337079355444303
2214	16	-1.6176995154366836
2214	17	-1.7806272793141247
2214	18	-1.821395310153406
2214	19	-3.5420258464349743
2214	20	-2.0338808161964295
2214	21	-2.419500729899049
2214	22	-2.1641203752686664
2214	23	-2.155038505610335
2214	24	-1.5095342907721374
2214	25	-1.4586273253210607
2215	1	-1.4940009760499713
2215	2	-1.2736377463256545
2215	3	-1.2098979662931555
2215	4	-1.2333630658747055
2215	5	-1.970957474141862
2215	6	-1.7718448257299606
2215	7	-1.8664380849304507
2215	8	-2.7317524164247793
2215	9	-2.1835509636843184
2215	10	-1.6585031289854584
2215	11	-2.2610925656109373
2215	12	-2.9897163582884367
2215	13	-2.211510479294539
2215	14	-2.23865120029658
2215	15	-3.381442363339758
2215	16	-2.8969106325186544
2215	17	-2.5461207426432964
2215	18	-2.9887803314135652
2215	19	-3.8661591149526444
2215	20	-3.2359242751542467
2215	21	-2.446766309223193
2215	22	-3.3849498391696784
2215	23	-3.315217768186951
2215	24	-2.301455798892462
2215	25	-2.469008652715047
2216	1	-1.0523018430349396
2216	2	-0.9321863479875923
2216	3	-1.2972938563840515
2216	4	-0.7594048561745197
2216	5	-0.7958528310470498
2216	6	-1.1981278206029202
2216	7	-2.359417632154152
2216	8	-1.4967478949190465
2216	9	-1.60624767646825
2216	10	-1.4925530002432836
2216	11	-2.0896856165436546
2216	12	-1.5096967934425507
2216	13	-1.9223918605042698
2216	14	-2.0222260065011994
2216	15	-2.134626357382267
2216	16	-1.9370441231201738
2216	17	-2.1242484782042865
2216	18	-1.772876818335945
2216	19	-1.875895154150001
2216	20	-2.4649211011418046
2216	21	-2.254920408032028
2216	22	-2.581743067070029
2216	23	-1.5404886151568817
2216	24	-1.810641463113619
2216	25	-1.656981420181232
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54646.0
3	1.0
4	897.0
5	8207.0
6	9447.0
7	7407.0
8	5874.0
9	5362.0
10	4417.0
11	4191.0
12	3888.0
13	3655.0
14	3532.0
15	3558.0
16	3507.0
17	3722.0
18	4180.0
19	4905.0
20	6310.0
21	8067.0
22	10148.0
23	12305.0
24	14762.0
25	16976.0
26	19139.0
27	20904.0
28	24161.0
29	28996.0
30	36159.0
31	46353.0
32	58658.0
33	75941.0
34	98512.0
35	129450.0
36	177289.0
37	275847.0
38	917686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.458835704873394	18.620104858023147	13.18568999739492	27.735369439708535
2	14.127674948875304	20.238030325372595	38.93575286419204	26.69854186156006
3	17.169742524983892	24.460908869785055	29.72226002212361	28.647088583107443
4	11.874683728604403	16.909226374674954	36.434512198649976	34.78157769807066
5	13.29478534651436	37.76296736310514	35.30841552496683	13.633831765413658
6	32.858589472625276	36.299588778873506	17.50275934785435	13.33906240064687
7	28.202946474560548	31.293984399677864	22.006926520263058	18.49614260549853
8	25.918269942422505	36.07650367592638	19.971125854458364	18.034100527192745
9	26.595496408283147	14.737328430535475	19.259372047221685	39.407803113959694
10	15.228179688419582	28.423217411444867	33.90065004347546	22.4479528566601
11	34.717742107556575	22.044266246121996	23.942217465911746	19.29577418040968
12	23.45087547254273	24.350722020633498	31.047219182057695	21.151183324766077
13	29.098917418742392	21.252799390784286	25.455572251665647	24.192710938807675
14	21.64009284439081	21.18137137519234	26.685033228144462	30.49350255227239
15	23.780413786824674	29.575534683286925	22.836277663553528	23.80777386633487
16	23.13076178914223	27.261751204522174	26.45979551576312	23.14769149057248
17	22.655242556045156	27.75781909896169	26.94212963292314	22.644808712070013
18	22.83206845964843	26.850251157162646	28.133916980158723	22.183763403030195
19	24.02017636777964	26.519273096943486	26.812065612076236	22.648484923200645
20	24.260656644061594	26.529588433383942	26.92447388910016	22.28528103345431
21	23.600971620912325	26.100197576827135	26.589990863197844	23.7088399390627
22	23.95279027546434	26.722084799035777	26.24627973544575	23.078845190054135
23	23.123535187967715	26.674218217698037	26.837324133653915	23.364922460680333
24	23.68152037544724	26.517431004742402	26.91002333743311	22.89102528237725
25	23.509003353260706	26.252683746548115	26.75453486784208	23.483778032349104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	329.5
3	445.0
4	445.0
5	445.0
6	1035.0
7	1625.0
8	1625.0
9	1625.0
10	2208.0
11	2791.0
12	2791.0
13	2791.0
14	4961.5
15	7132.0
16	7132.0
17	7132.0
18	13308.5
19	19485.0
20	19485.0
21	19485.0
22	31210.0
23	42935.0
24	42935.0
25	42935.0
26	62490.5
27	82046.0
28	82046.0
29	82046.0
30	106927.5
31	131809.0
32	131809.0
33	131809.0
34	153185.0
35	174561.0
36	174561.0
37	174561.0
38	194923.5
39	215286.0
40	215286.0
41	215286.0
42	232587.0
43	249888.0
44	249888.0
45	249888.0
46	268267.5
47	286647.0
48	286647.0
49	286647.0
50	280930.0
51	275213.0
52	275213.0
53	275213.0
54	251049.0
55	226885.0
56	226885.0
57	226885.0
58	197679.5
59	168474.0
60	168474.0
61	168474.0
62	140470.0
63	112466.0
64	112466.0
65	112466.0
66	87869.0
67	63272.0
68	63272.0
69	63272.0
70	46504.5
71	29737.0
72	29737.0
73	29737.0
74	20786.5
75	11836.0
76	11836.0
77	11836.0
78	8117.0
79	4398.0
80	4398.0
81	4398.0
82	2884.5
83	1371.0
84	1371.0
85	1371.0
86	904.5
87	438.0
88	438.0
89	438.0
90	266.5
91	95.0
92	95.0
93	95.0
94	52.0
95	9.0
96	9.0
97	9.0
98	5.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0776649681208539
2	0.0
3	0.0
4	0.02399174228885963
5	0.06557426795551949
6	0.07951413402849328
7	0.030392701199919017
8	0.06121213299390865
9	0.04148769664575529
10	0.1006610056902154
11	0.0022758965017100046
12	0.012090700165334398
13	0.005594912233370428
14	0.007301834609652931
15	0.006969933036486888
16	0.016073519043326906
17	0.025414177602428385
18	9.95704719498127E-4
19	0.00270262709578063
20	0.004409549472063133
21	2.844870627137506E-4
22	9.482902090458351E-5
23	0.0
24	4.7414510452291754E-5
25	0.003176772200303548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2109059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.09777116063944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8287829485843	53.56333410316465
2	12.838171730531995	17.228254177924526
3	3.6988179509848997	7.445473213201503
4	1.442451921136048	3.871412356584452
5	0.7106132428345859	2.3840282375717474
6	0.4196493851822972	1.689452304879889
7	0.24721736179499224	1.1611413778060178
8	0.16822515818235906	0.9030026533745841
9	0.11741510931222798	0.7090462921890005
>10	0.4912841358983671	5.545741683438219
>50	0.020579182598102725	0.9346054976092187
>100	0.01353468060618355	1.8582371619609646
>500	0.0018410217651258444	0.8515861788567267
>1k	0.0014161705885580463	1.8546847614385662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4047	0.19188652380042476	No Hit
CCCATGTACTCTGCGTTGATACCAC	3988	0.18908906768373954	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3695	0.17519661612121804	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2581	0.12237685147736502	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2482	0.11768281494258814	No Hit
GTCCTACAGTGGACATTTCTAAATT	2446	0.11597589256630562	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2346	0.11123444152107645	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2287	0.10843698540439124	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2207	0.10464382456820791	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.7414510452291754E-5	0.0
10	0.0	0.0	0.0	4.7414510452291754E-5	0.0
11	0.0	0.0	0.0	4.7414510452291754E-5	0.0
12	0.0	0.0	0.0	4.7414510452291754E-5	0.0
13	0.0	0.0	0.0	4.7414510452291754E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTCCG	50	8.606854E-5	15.222531	5
TAGGACC	460	0.0	12.202844	4
GTGCGCC	100	1.9479667E-6	11.391448	11
ATAGGAC	195	0.0	11.212677	3
TGGATCG	60	0.0058123926	11.099762	5
ATAAGCG	60	0.005847447	11.090801	3
AGGACGT	1380	0.0	10.961877	5
GGACGTG	1355	0.0	10.880424	6
GGATCGT	80	3.7295953E-4	10.700544	6
AATCCCG	125	2.2709719E-7	10.629749	19
GTAGGAC	1400	0.0	10.524942	3
TAGGACG	1520	0.0	10.515565	4
GTCTAGA	200	1.8189894E-12	10.457785	1
TTAGTAC	155	3.623427E-9	10.426374	3
GACGTGG	745	0.0	10.333855	7
TTAGGAC	970	0.0	10.192429	3
TGGCGAG	1240	0.0	10.103162	18
GGCGAGG	630	0.0	10.093442	19
GTCCTAC	1335	0.0	10.041183	1
CGTGGAA	765	0.0	9.9363785	9
>>END_MODULE