FastQCFastQC Report
Thu 26 May 2016
SRR1513015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156820
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68390.3170871931825558No Hit
GTCCTAAAGTGTGTATTTCTCATTT63930.2964086015522853No Hit
CTGTAGGACGTGGAATATGGCAAGA59850.27749186302055806No Hit
CTTTAGGACGTGAAATATGGCGAGG56680.2627942990142895No Hit
GTCCTACAGTGTGCATTTCTCATTT37030.17168794799751486No Hit
GTACATGGGAAGCAGTGGTATCAAC34550.16018953830175905No Hit
GTATCAACGCAGAGTACTTTTTTTT32980.15291030313146206No Hit
CTGAAGGACCTGGAATATGGCGAGA30280.1403918732207602No Hit
CCCATGTACTCTGCGTTGATACCAC27640.12815163064140725No Hit
GTCCTTCAGTGTGCATTTCTCATTT25500.11822961582329541No Hit
CTGTAGGACCTGGAATATGGCGAGA25460.11804415760239612No Hit
ATTTAGAAATGTCCACTGTAGGACG24410.11317587930378985No Hit
TTTCTAAATTTTCCACCTTTTTCAG24050.11150675531569625No Hit
GAATATGGCAAGAAAACTGAAAATC22240.10311477082000353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTC551.970475E-413.8100876
AAATGTC7950.013.0175297
TAGGACC9600.012.1672854
GATATAC7350.011.6718431
GAAATGT9350.011.5761046
AATGTCC8600.011.481918
TATGTCG1251.8255378E-811.40310516
CCACCTT9400.011.221413
AAGTCGT2650.011.1066987
TCTAGGC1803.6379788E-1211.0791533
TGTCCAC9600.011.07761210
ATTTAGA9800.010.9909861
AATCCCG4250.010.94703319
TTCGCAT700.001488422710.860117
TTTAGAA10550.010.8016122
GGCGAGG15150.010.77966119
GCGCCAC1252.2320091E-710.64314513
TAAGACG909.554542E-510.5515754
TAGAAAT10550.010.5315714
ACTGTTC8050.010.37925158
Produced by FastQC (version 0.11.5)