##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513015_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2156820 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.271875260800623 31.0 30.0 34.0 23.0 34.0 2 29.269000658376683 31.0 30.0 34.0 23.0 34.0 3 29.49977513190716 31.0 30.0 34.0 24.0 34.0 4 32.93318357581996 35.0 35.0 37.0 26.0 37.0 5 33.06149145501247 37.0 35.0 37.0 26.0 37.0 6 33.062719652080375 37.0 35.0 37.0 26.0 37.0 7 32.86646219897813 37.0 35.0 37.0 26.0 37.0 8 32.88954572008791 37.0 35.0 37.0 26.0 37.0 9 34.21984078411736 39.0 35.0 39.0 25.0 39.0 10 34.083922626830244 39.0 34.0 39.0 25.0 39.0 11 34.168320026705985 39.0 35.0 39.0 25.0 39.0 12 34.07222670412923 39.0 35.0 39.0 25.0 39.0 13 34.08428148848768 39.0 35.0 39.0 25.0 39.0 14 35.08877606847118 39.0 35.0 41.0 25.0 41.0 15 35.056823935237986 40.0 35.0 41.0 24.0 41.0 16 34.94520961415417 39.0 35.0 41.0 23.0 41.0 17 34.90887510316114 39.0 35.0 41.0 23.0 41.0 18 34.60232750067229 39.0 34.0 41.0 18.0 41.0 19 34.83792713346501 39.0 34.0 41.0 21.0 41.0 20 34.92990745634777 40.0 35.0 41.0 22.0 41.0 21 34.8558470340594 40.0 35.0 41.0 22.0 41.0 22 34.78579297298801 39.0 35.0 41.0 19.0 41.0 23 34.526277111673664 39.0 34.0 41.0 18.0 41.0 24 34.53954432915125 39.0 34.0 41.0 18.0 41.0 25 34.463382665220095 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7741705336282756 1101 2 1.055417201474345 1101 3 0.9639869201521662 1101 4 1.0382029960138226 1101 5 0.8777643964994297 1101 6 1.0339672352329927 1101 7 1.0352646013738607 1101 8 0.7682930285778085 1101 9 0.7326504968757774 1101 10 0.7576873667792796 1101 11 0.9746744202130699 1101 12 1.0988274005682541 1101 13 0.9644808480537748 1101 14 0.9895732043908154 1101 15 0.7692372057679435 1101 16 1.2076144808188118 1101 17 1.2234161642705388 1101 18 1.5340734962533702 1101 19 1.0062982175412003 1101 20 1.267405803886227 1101 21 1.3743512475385202 1101 22 1.2504755946223582 1101 23 1.457429046353596 1101 24 1.2165902316130044 1101 25 1.4376049478495503 1102 1 0.7989304164261952 1102 2 0.8789298323745172 1102 3 0.9115433245973996 1102 4 0.9963458633922002 1102 5 0.8527680732057021 1102 6 0.802492555483532 1102 7 1.0408481040870825 1102 8 0.9912098445124613 1102 9 1.1067657305068934 1102 10 1.1373851603784146 1102 11 1.0001404191647367 1102 12 1.1777278202784132 1102 13 0.9843278970727525 1102 14 1.0013188668201565 1102 15 1.2927598297672205 1102 16 1.1799782824199667 1102 17 1.1887772545365038 1102 18 1.4023427669079709 1102 19 1.4298958840582827 1102 20 1.4131787229647514 1102 21 1.1846933509161843 1102 22 1.47974783303885 1102 23 1.6833850842594913 1102 24 1.4911361112508388 1102 25 1.4032522555578097 1103 1 0.3175280494547188 1103 2 0.47904272709700635 1103 3 0.4079174224394926 1103 4 0.6234707747671138 1103 5 0.21588185872391108 1103 6 0.5028476012049907 1103 7 0.37827370446521513 1103 8 0.4498909214663058 1103 9 0.6365273972038565 1103 10 0.549878115820448 1103 11 0.6208968683439338 1103 12 0.7726973696085011 1103 13 0.7325257627989288 1103 14 0.8774489957128466 1103 15 0.928274218844976 1103 16 0.9612746628806406 1103 17 0.8663984663822717 1103 18 1.1293944148800534 1103 19 1.0657640462921805 1103 20 0.9731227300911129 1103 21 0.8133651563227744 1103 22 0.9667807561213664 1103 23 1.255796959111784 1103 24 1.0599458990960215 1103 25 1.0330515538289404 1104 1 0.557815828886401 1104 2 0.5790841194166312 1104 3 0.5896538436577501 1104 4 0.760314230444699 1104 5 0.5402072220242147 1104 6 0.5432805666562572 1104 7 0.7126499812126568 1104 8 0.6770892514694822 1104 9 0.8228737427447967 1104 10 0.8924643758947397 1104 11 0.8710933254116924 1104 12 0.8370938955144638 1104 13 0.8639482550099373 1104 14 1.0054707914958385 1104 15 1.151892604902109 1104 16 1.000169675687225 1104 17 0.9641668571329163 1104 18 1.2581436605653735 1104 19 1.0588163052132131 1104 20 1.0746074001818116 1104 21 1.1359887857050595 1104 22 1.0243121467870822 1104 23 1.3050232445757501 1104 24 1.223619087511537 1104 25 0.9915091311713411 1105 1 0.7417053691343476 1105 2 0.8538271011906637 1105 3 0.9354100043728764 1105 4 1.0469957507838927 1105 5 0.7932595442871246 1105 6 0.5819895595745734 1105 7 0.8058158145426333 1105 8 0.7855092424176036 1105 9 0.7238776414980777 1105 10 0.9529078103968942 1105 11 0.8991691838962197 1105 12 0.9828916894889161 1105 13 0.9938139407432871 1105 14 1.2193792547316562 1105 15 1.3446707117169368 1105 16 1.1889238608370434 1105 17 0.9886227708688082 1105 18 1.1008706659118843 1105 19 1.0951975960322713 1105 20 1.1403815034887046 1105 21 1.0358736898881276 1105 22 0.7813339988761996 1105 23 1.044676775277594 1105 24 1.2201721222403847 1105 25 1.1375073019939421 1106 1 0.5857413501526523 1106 2 0.7313624120944802 1106 3 0.7040313270696394 1106 4 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1107 17 1.5116993249830841 1107 18 1.7093320661801457 1107 19 1.6382548594376587 1107 20 1.4842378661958904 1107 21 1.4582483831292308 1107 22 1.4234851571036202 1107 23 1.5427495357850134 1107 24 1.449349406645787 1107 25 1.5469051562950256 1108 1 0.7359040992349719 1108 2 0.7130194905102591 1108 3 0.5975958524468901 1108 4 0.721996513420784 1108 5 0.7140586134343252 1108 6 0.7939036910183148 1108 7 0.7444387973680477 1108 8 0.8081146715206984 1108 9 1.0003801451260514 1108 10 0.9078520943244328 1108 11 0.844181320717631 1108 12 0.8480326465764279 1108 13 0.9741749512589664 1108 14 1.217067811256122 1108 15 1.2030438111168493 1108 16 0.9986551075958374 1108 17 1.2024203975427952 1108 18 1.2896250401792102 1108 19 1.2712207278987293 1108 20 1.2478488081556023 1108 21 1.159529378941457 1108 22 1.1277459012412479 1108 23 1.2931591278598802 1108 24 1.3441128195955656 1108 25 1.4369956815866516 1109 1 0.046796515332289346 1109 2 0.0016416859157573072 1109 3 0.20821388837032018 1109 4 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6 -1.5813488477429338 2214 7 -1.7318016070506026 2214 8 -1.5117933178873848 2214 9 -1.6812141701885395 2214 10 -1.67607012462868 2214 11 -1.4919060893446883 2214 12 -1.9329295417064856 2214 13 -1.7496934165337663 2214 14 -3.0472887832820916 2214 15 -3.173701225036538 2214 16 -2.4736534488542645 2214 17 -2.8154227030001735 2214 18 -2.2707863327579076 2214 19 -4.29133577569878 2214 20 -2.63767557197653 2214 21 -2.539339211745144 2214 22 -2.603895221787255 2214 23 -2.766879566516952 2214 24 -3.0029570175135945 2214 25 -2.8340825006692114 2215 1 -0.9196429858282329 2215 2 -2.653352540381853 2215 3 -1.3238425058343317 2215 4 -0.9895320556039984 2215 5 -0.9976696417243787 2215 6 -1.4724511699568694 2215 7 -1.8927454049347823 2215 8 -1.828117973985762 2215 9 -1.6881240803905015 2215 10 -3.1944787218990776 2215 11 -2.2333882432008174 2215 12 -2.329988142758989 2215 13 -1.649948438632073 2215 14 -2.9796657837467464 2215 15 -2.8324989471272843 2215 16 -2.5120105014039424 2215 17 -2.7008367356991982 2215 18 -1.735155099034479 2215 19 -2.2849391514997777 2215 20 -2.381477238264985 2215 21 -2.1133950356502282 2215 22 -1.7381254548731349 2215 23 -2.358451410348337 2215 24 -2.0988296577487304 2215 25 -2.0541454997723108 2216 1 -2.1227883166656234 2216 2 -2.0770101523883078 2216 3 -2.59170933161554 2216 4 -3.1675051562270333 2216 5 -2.284309675379429 2216 6 -2.3880223386559827 2216 7 -2.1752471745379154 2216 8 -2.780949462316247 2216 9 -3.04404059740283 2216 10 -2.5968798681156855 2216 11 -3.046363301045435 2216 12 -3.4439523462156103 2216 13 -4.013327501540456 2216 14 -3.3559762501785464 2216 15 -3.3571625815979225 2216 16 -2.499903562058087 2216 17 -2.672824860596357 2216 18 -2.5983880309524103 2216 19 -2.989205348433263 2216 20 -2.7883870408269544 2216 21 -3.163120795610652 2216 22 -3.599730510459281 2216 23 -3.640905350459118 2216 24 -3.6528451858620805 2216 25 -3.701209209360794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97232.0 3 2.0 4 828.0 5 4774.0 6 6146.0 7 5475.0 8 4867.0 9 4568.0 10 4133.0 11 3984.0 12 3841.0 13 3722.0 14 3828.0 15 3955.0 16 3959.0 17 4227.0 18 4804.0 19 5805.0 20 7369.0 21 9473.0 22 11943.0 23 14682.0 24 16543.0 25 18694.0 26 21376.0 27 23872.0 28 27228.0 29 31987.0 30 39476.0 31 48971.0 32 62084.0 33 79970.0 34 104498.0 35 138097.0 36 191094.0 37 296086.0 38 847227.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60079273564528 19.236775563118712 12.680001154017608 25.4824305472184 2 13.89287526542774 20.504843795368547 42.99702127225349 22.60525966695022 3 18.40854561552986 25.405358773647958 30.099631131516713 26.08646447930547 4 11.796875217411067 17.39988887105796 39.24238446717939 31.560851444351577 5 12.19222356012297 38.79977624692247 36.66795303040704 12.34004716254752 6 30.71551690957462 38.01652815912886 18.839560182727002 12.428394748569518 7 26.97654834180101 31.82144056211267 23.33869403033524 17.863317065751083 8 23.712636332691186 38.89234644804587 21.141758173468887 16.253259045794056 9 26.335591297770737 15.21396867972845 20.773133701266993 37.677306321233814 10 14.571735964904144 29.459464472866564 35.594654003468776 20.374145558760514 11 33.285305734235635 22.262670312868813 26.25376843524784 18.198255517647706 12 23.369905207445953 24.864066985934382 32.971587275603284 18.794440531016377 13 28.981234798053222 22.730124722626062 26.001293832038343 22.287346647282373 14 19.92199347935981 23.53051390621594 28.129559462580524 28.417933151843727 15 22.104809143843003 33.07343225713696 23.348846700568824 21.472911898451212 16 20.658675414750242 28.507464901468122 29.93501079134355 20.89884889243808 17 20.742984952667243 29.661985717392845 28.963437397539987 20.631591932399925 18 20.690437810423404 27.6988763606878 31.826901342621106 19.783784486267688 19 23.159768823871506 27.15911399179512 27.973109218755653 21.70800796557772 20 23.1990001530378 28.27517124002356 28.73725264685832 19.78857596008032 21 21.942519945405493 27.38328059543812 27.622630099046454 23.05156936010993 22 22.504761414526175 29.549289857108874 27.5573097161608 20.388639012204155 23 21.04797944665498 29.173599710620774 28.632822282200394 21.14559856052385 24 22.7416209698923 27.617746965463674 29.312589123201594 20.328042941442433 25 21.931326869576147 28.212134407936446 28.775266876931234 21.08127184555618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 296.0 1 296.0 2 470.5 3 645.0 4 645.0 5 645.0 6 1297.5 7 1950.0 8 1950.0 9 1950.0 10 2878.5 11 3807.0 12 3807.0 13 3807.0 14 6745.0 15 9683.0 16 9683.0 17 9683.0 18 18200.0 19 26717.0 20 26717.0 21 26717.0 22 44111.5 23 61506.0 24 61506.0 25 61506.0 26 90564.0 27 119622.0 28 119622.0 29 119622.0 30 157197.5 31 194773.0 32 194773.0 33 194773.0 34 216508.5 35 238244.0 36 238244.0 37 238244.0 38 250115.0 39 261986.0 40 261986.0 41 261986.0 42 269304.0 43 276622.0 44 276622.0 45 276622.0 46 281570.0 47 286518.0 48 286518.0 49 286518.0 50 266094.0 51 245670.0 52 245670.0 53 245670.0 54 209888.0 55 174106.0 56 174106.0 57 174106.0 58 146718.0 59 119330.0 60 119330.0 61 119330.0 62 95626.5 63 71923.0 64 71923.0 65 71923.0 66 54604.5 67 37286.0 68 37286.0 69 37286.0 70 26887.5 71 16489.0 72 16489.0 73 16489.0 74 11320.5 75 6152.0 76 6152.0 77 6152.0 78 4257.0 79 2362.0 80 2362.0 81 2362.0 82 1573.0 83 784.0 84 784.0 85 784.0 86 513.5 87 243.0 88 243.0 89 243.0 90 154.0 91 65.0 92 65.0 93 65.0 94 44.0 95 23.0 96 23.0 97 23.0 98 20.5 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36192171808495843 2 0.04015170482469562 3 0.035561613857438265 4 0.03551524930221344 5 0.04042989215604455 6 0.017572166430207436 7 0.017803989206331545 8 0.02383138138555837 9 0.021003143516844243 10 0.02040040429892156 11 0.01951947774964995 12 0.01979766508099888 13 0.020168581522797453 14 0.02735508758264482 15 0.028514201463265366 16 0.021420424513867636 17 0.02276499661538747 18 0.10677757068276443 19 0.025129588931853377 20 0.022718632060162648 21 0.025917786370675343 22 0.019612206860099592 23 0.02225498650791443 24 0.018313999313804584 25 0.022672267504937824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2156820.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.229403395509 #Duplication Level Percentage of deduplicated Percentage of total 1 84.77266114959187 59.535334167854806 2 10.683140116174735 15.005411134991606 3 2.4567455165708565 5.176073157700884 4 0.8379549934647404 2.353963170532654 5 0.3741845026096245 1.3139377189059605 6 0.20358442937978258 0.8578567809574356 7 0.13097991336809645 0.6439048820845806 8 0.09149110559495544 0.5140292609543389 9 0.060323835380886995 0.38128562723957315 >10 0.33853944167920885 4.31234293364968 >50 0.023252737686718445 1.1266263225863336 >100 0.02138574043445394 3.127398300677852 >500 0.003176009966382115 1.5686870347888546 >1k 0.0023158406004867684 2.926778369546037 >5k 2.6466749719848784E-4 1.1563711375294223 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6839 0.3170871931825558 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6393 0.2964086015522853 No Hit CTGTAGGACGTGGAATATGGCAAGA 5985 0.27749186302055806 No Hit CTTTAGGACGTGAAATATGGCGAGG 5668 0.2627942990142895 No Hit GTCCTACAGTGTGCATTTCTCATTT 3703 0.17168794799751486 No Hit GTACATGGGAAGCAGTGGTATCAAC 3455 0.16018953830175905 No Hit GTATCAACGCAGAGTACTTTTTTTT 3298 0.15291030313146206 No Hit CTGAAGGACCTGGAATATGGCGAGA 3028 0.1403918732207602 No Hit CCCATGTACTCTGCGTTGATACCAC 2764 0.12815163064140725 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2550 0.11822961582329541 No Hit CTGTAGGACCTGGAATATGGCGAGA 2546 0.11804415760239612 No Hit ATTTAGAAATGTCCACTGTAGGACG 2441 0.11317587930378985 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2405 0.11150675531569625 No Hit GAATATGGCAAGAAAACTGAAAATC 2224 0.10311477082000353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.272911044964346E-5 0.0 0.0 4.636455522482173E-5 6 0.0 9.272911044964346E-5 0.0 0.0 4.636455522482173E-5 7 0.0 9.272911044964346E-5 0.0 0.0 4.636455522482173E-5 8 0.0 9.272911044964346E-5 0.0 0.0 4.636455522482173E-5 9 0.0 9.272911044964346E-5 0.0 0.0 4.636455522482173E-5 10 4.636455522482173E-5 9.272911044964346E-5 0.0 0.0 4.636455522482173E-5 11 4.636455522482173E-5 9.272911044964346E-5 0.0 0.0 4.636455522482173E-5 12 9.272911044964346E-5 9.272911044964346E-5 0.0 0.0 1.8545822089928693E-4 13 9.272911044964346E-5 9.272911044964346E-5 0.0 0.0 2.3182277612410863E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGTC 55 1.970475E-4 13.810087 6 AAATGTC 795 0.0 13.017529 7 TAGGACC 960 0.0 12.167285 4 GATATAC 735 0.0 11.671843 1 GAAATGT 935 0.0 11.576104 6 AATGTCC 860 0.0 11.48191 8 TATGTCG 125 1.8255378E-8 11.403105 16 CCACCTT 940 0.0 11.2214 13 AAGTCGT 265 0.0 11.106698 7 TCTAGGC 180 3.6379788E-12 11.079153 3 TGTCCAC 960 0.0 11.077612 10 ATTTAGA 980 0.0 10.990986 1 AATCCCG 425 0.0 10.947033 19 TTCGCAT 70 0.0014884227 10.8601 17 TTTAGAA 1055 0.0 10.801612 2 GGCGAGG 1515 0.0 10.779661 19 GCGCCAC 125 2.2320091E-7 10.643145 13 TAAGACG 90 9.554542E-5 10.551575 4 TAGAAAT 1055 0.0 10.531571 4 ACTGTTC 805 0.0 10.3792515 8 >>END_MODULE