FastQCFastQC Report
Thu 26 May 2016
SRR1513015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156820
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62970.2919576042507024No Hit
CTGTAGGACGTGGAATATGGCAAGA59940.27790914401758143No Hit
GTCCTAAAGTGTGTATTTCTCATTT58620.271789022727905No Hit
CTTTAGGACGTGAAATATGGCGAGG58320.2703980860711603No Hit
GTATCAACGCAGAGTACTTTTTTTT39030.18096085904247922No Hit
GTCCTACAGTGTGCATTTCTCATTT35460.16440871282721786No Hit
CCCATGTACTCTGCGTTGATACCAC33340.15457942711955563No Hit
GTACATGGGAAGCAGTGGTATCAAC32830.15221483480308973No Hit
CTGAAGGACCTGGAATATGGCGAGA31650.14674381728656077No Hit
TATCAACGCAGAGTACTTTTTTTTT28210.1307944102892221No Hit
TTTCTAAATTTTCCACCTTTTTCAG25790.11957418792481524No Hit
ATTTAGAAATGTCCACTGTAGGACG25470.11809052215762095No Hit
CTGTAGGACCTGGAATATGGCGAGA25400.11776597027104718No Hit
GGTATCAACGCAGAGTACTTTTTTT25040.11609684628295361No Hit
GTCCTTCAGTGTGCATTTCTCATTT23880.11071855787687429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT801.2702549E-714.2672716
TCCAACG2050.013.42442918
TAGGACC9850.013.2332114
AATGGCG853.9780316E-612.28503816
CCAACGT853.9924944E-612.28105119
GAACCGT550.003041389612.1055636
CCAACGA1353.783498E-1011.95024819
GTCGAGG1451.0004442E-1011.7805719
AATCCCG3950.011.772284519
GCCGGTT2050.011.57707811
AGGACCG1001.8956962E-611.4172665
GGCGAGG17100.011.09930119
ATGTCGA2150.011.03835317
GTATTAG1900.011.0077061
AAATGTC8300.010.8775747
CGGGAGT700.001473090110.8735874
TTCGGGA700.001482544410.8652522
TATGTCG2100.010.84912416
TGTCGAG2200.010.783730518
ACGAAGG803.7977862E-410.67960716
Produced by FastQC (version 0.11.5)