##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513015_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2156820 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.472080655780267 31.0 31.0 34.0 27.0 34.0 2 30.6270680909858 31.0 31.0 34.0 27.0 34.0 3 30.72603184317653 31.0 31.0 34.0 27.0 34.0 4 34.1802741999796 37.0 35.0 37.0 30.0 37.0 5 33.834902773527695 37.0 35.0 37.0 30.0 37.0 6 33.69797525987333 37.0 35.0 37.0 29.0 37.0 7 33.56747619180089 37.0 35.0 37.0 28.0 37.0 8 33.450398735174936 37.0 35.0 37.0 28.0 37.0 9 34.911502582505726 39.0 35.0 39.0 28.0 39.0 10 34.79331562207324 39.0 35.0 39.0 27.0 39.0 11 34.818235179569925 39.0 35.0 39.0 27.0 39.0 12 34.677419070668854 39.0 35.0 39.0 27.0 39.0 13 34.66250359325303 39.0 35.0 39.0 27.0 39.0 14 35.70704277593865 40.0 36.0 41.0 27.0 41.0 15 35.73586251982085 40.0 36.0 41.0 27.0 41.0 16 35.71505688930926 40.0 36.0 41.0 27.0 41.0 17 35.69907409983216 40.0 36.0 41.0 27.0 41.0 18 35.62544394061628 40.0 36.0 41.0 27.0 41.0 19 35.61230468931112 40.0 36.0 41.0 27.0 41.0 20 35.58853682736621 40.0 36.0 41.0 27.0 41.0 21 35.53478732578518 40.0 36.0 41.0 26.0 41.0 22 35.49386736028041 40.0 36.0 41.0 26.0 41.0 23 35.386527851188326 40.0 36.0 41.0 26.0 41.0 24 35.37327547036841 40.0 36.0 41.0 26.0 41.0 25 35.282899361096426 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7346177340348703 1101 2 0.7989404898645418 1101 3 0.7636310528942793 1101 4 0.5193751096017039 1101 5 0.8227008021097291 1101 6 0.8766262933750752 1101 7 0.912776161067427 1101 8 0.8818637200277308 1101 9 0.5554309976531258 1101 10 0.8423795200665296 1101 11 0.8339013094560883 1101 12 0.9823821444929521 1101 13 0.9699131576172135 1101 14 1.1186172017308706 1101 15 1.0349626159572836 1101 16 1.2189899674352915 1101 17 1.1689067285101942 1101 18 0.5980863380065955 1101 19 0.9504710808216785 1101 20 1.0535798802984147 1101 21 1.1088818099799909 1101 22 1.0733517647395843 1101 23 1.010076779914428 1101 24 0.9468518650742226 1101 25 1.0191021673953031 1102 1 0.627501516177766 1102 2 0.5906056795637298 1102 3 0.5463812668974377 1102 4 0.5251401850401791 1102 5 0.6847304193928849 1102 6 0.5467857681528656 1102 7 0.6051642477848986 1102 8 0.6940781153698197 1102 9 0.8719009448216823 1102 10 0.9983914061979959 1102 11 0.9574369433391041 1102 12 0.9443001407445948 1102 13 0.9608722311626536 1102 14 1.0469861016980602 1102 15 1.0399987608081958 1102 16 1.0406485956278928 1102 17 1.0861842312158743 1102 18 0.9587891356972449 1102 19 1.1105216873839439 1102 20 1.0785122854676814 1102 21 1.0338749279400403 1102 22 0.9833616447092695 1102 23 1.1177250831616234 1102 24 1.0934044344319886 1102 25 1.0842047569278108 1103 1 0.5921834657478229 1103 2 0.39819653163710456 1103 3 0.5007134428023221 1103 4 0.5472573701257133 1103 5 0.5289066151497934 1103 6 0.6153352254946611 1103 7 0.5843532600924206 1103 8 0.564458794192511 1103 9 0.5813595591798588 1103 10 0.724464451446778 1103 11 0.659135818027778 1103 12 0.7563713316839298 1103 13 0.7439162073838617 1103 14 0.7827057373807946 1103 15 0.6080562807602021 1103 16 0.7322416656008102 1103 17 0.8069095934424979 1103 18 0.7881015868398293 1103 19 0.7825481567423864 1103 20 0.8151241712276729 1103 21 0.5210392888395674 1103 22 0.8011610156984119 1103 23 0.8456767983978608 1103 24 0.8915143113567439 1103 25 0.9733621368976344 1104 1 0.4840165733455031 1104 2 0.6327913026159919 1104 3 0.5607600203369323 1104 4 0.5026515582149145 1104 5 0.5648817261178607 1104 6 0.6996360931241696 1104 7 0.5968491812056342 1104 8 0.6510450900269049 1104 9 0.6773532654016705 1104 10 0.8076518388583267 1104 11 0.8514898609309611 1104 12 0.8072427607023585 1104 13 0.8471149486282243 1104 14 0.9544086782022916 1104 15 0.9281370985937656 1104 16 0.8274701950112586 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1107 17 0.9072121065479166 1107 18 0.9639000159312445 1107 19 1.0973660741564828 1107 20 1.1110145559435338 1107 21 1.0263702768400549 1107 22 1.025529604493066 1107 23 1.0938658995664667 1107 24 1.1043844414530497 1107 25 1.1080525703778434 1108 1 0.6761362963470106 1108 2 0.6054068358014142 1108 3 0.6030022901109717 1108 4 0.6706897708045361 1108 5 0.6945129856297498 1108 6 0.7683050701489407 1108 7 0.7436718276047145 1108 8 0.7464378203445534 1108 9 0.678978461751548 1108 10 0.906414758221942 1108 11 0.9036465007656886 1108 12 0.9586036105229709 1108 13 0.9149618537874105 1108 14 1.0355737587897806 1108 15 1.018472711724911 1108 16 0.9141275713490344 1108 17 0.9561319945564577 1108 18 1.0381986235223053 1108 19 1.0599259906051444 1108 20 1.1132365249111515 1108 21 1.126339319299511 1108 22 1.1121083199932684 1108 23 1.0819232628685143 1108 24 1.1227601231142827 1108 25 1.1725379778096894 1109 1 0.1054211133705465 1109 2 0.14503903633040593 1109 3 0.1358757223945517 1109 4 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-2.8369223239758057 2216 22 -3.14139000425417 2216 23 -2.2363949844765756 2216 24 -2.4288835795357855 2216 25 -2.3150285748145194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55864.0 3 2.0 4 958.0 5 8154.0 6 9309.0 7 7427.0 8 5883.0 9 5334.0 10 4593.0 11 3978.0 12 3752.0 13 3471.0 14 3469.0 15 3462.0 16 3406.0 17 3522.0 18 4130.0 19 4840.0 20 6247.0 21 8054.0 22 10041.0 23 12199.0 24 14559.0 25 16721.0 26 19208.0 27 21200.0 28 23794.0 29 28940.0 30 35967.0 31 46273.0 32 58765.0 33 76679.0 34 100095.0 35 130586.0 36 181154.0 37 282590.0 38 952194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.809833925027526 19.94124664576442 13.65052658540558 27.59839284380248 2 12.697739307507314 20.91234444090528 41.974632061969096 24.415284189618312 3 16.81035042330839 25.422195639877227 30.573483183575824 27.193970753238567 4 11.012832582794971 17.956850614266568 38.54454302184832 32.485773781090145 5 11.845423906225061 38.83253653340064 36.88031483495477 12.441724725419526 6 30.713187301687473 38.38857237253011 18.49708917347004 12.401151152312375 7 26.701358935114328 31.85158387829878 23.345855943601872 18.101201242985017 8 23.670320860080952 38.972278980686035 20.938305560349868 16.41909459888314 9 25.90754452485942 14.974101964873674 20.696950776569956 38.421402733696944 10 14.241401717335808 29.654537015548847 35.60023207240659 20.50382919470875 11 33.12494754807842 22.188055381810578 26.283733250739427 18.403263819371578 12 23.167697744612635 24.858693399107157 32.9635642512037 19.010044605076512 13 28.773400746153083 22.559585136660264 26.14967018475774 22.51734393242891 14 19.690671934193016 23.42625694956436 28.11018218407593 28.77288893216669 15 21.88480262483719 33.07054667260804 23.34249423521616 21.70215646733861 16 20.647713386710446 28.459182277500688 29.79472303957372 21.09838129621514 17 20.627290853191067 29.68519045010758 28.91733286830241 20.770185828398947 18 20.52440114540909 27.868391085987287 31.69909457785295 19.908113190750672 19 23.03824994725824 27.17728418187677 28.01619114077529 21.768274730089697 20 23.073267176153 28.287295020777286 28.720599513511957 19.918838289557755 21 21.71646008852885 27.29075473001575 27.810457198150417 23.182327983304983 22 22.455813194399692 29.41424113122673 27.6126907853222 20.517254889051372 23 20.88956004189503 28.972250337186384 28.690307346142628 21.447882274775953 24 22.602313870565865 27.659437347315652 29.254888796880962 20.483359985237517 25 21.85685909396085 28.11578465156478 28.806182328045075 21.221173926429294 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 151.0 1 151.0 2 340.0 3 529.0 4 529.0 5 529.0 6 1196.0 7 1863.0 8 1863.0 9 1863.0 10 2828.0 11 3793.0 12 3793.0 13 3793.0 14 6600.0 15 9407.0 16 9407.0 17 9407.0 18 18034.0 19 26661.0 20 26661.0 21 26661.0 22 44359.0 23 62057.0 24 62057.0 25 62057.0 26 91504.0 27 120951.0 28 120951.0 29 120951.0 30 157678.5 31 194406.0 32 194406.0 33 194406.0 34 215964.0 35 237522.0 36 237522.0 37 237522.0 38 249737.0 39 261952.0 40 261952.0 41 261952.0 42 269594.5 43 277237.0 44 277237.0 45 277237.0 46 282266.0 47 287295.0 48 287295.0 49 287295.0 50 267345.0 51 247395.0 52 247395.0 53 247395.0 54 211035.0 55 174675.0 56 174675.0 57 174675.0 58 146314.5 59 117954.0 60 117954.0 61 117954.0 62 94493.0 63 71032.0 64 71032.0 65 71032.0 66 53962.5 67 36893.0 68 36893.0 69 36893.0 70 26429.5 71 15966.0 72 15966.0 73 15966.0 74 10977.5 75 5989.0 76 5989.0 77 5989.0 78 4077.0 79 2165.0 80 2165.0 81 2165.0 82 1428.0 83 691.0 84 691.0 85 691.0 86 439.0 87 187.0 88 187.0 89 187.0 90 116.0 91 45.0 92 45.0 93 45.0 94 24.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07923702487922034 2 1.390936656744652E-4 3 0.0 4 0.02735508758264482 5 0.07103049860442688 6 0.0816943463061359 7 0.03338247976187164 8 0.06899045817453472 9 0.049053699427861384 10 0.10756576812158641 11 0.0038482580836602036 12 0.015717584221214567 13 0.007325599725521834 14 0.009736556597212563 15 0.00885563004794095 16 0.021559518179542103 17 0.03203790766035181 18 0.001669123988093582 19 0.003940987194109847 20 0.006398308621025398 21 4.172809970233955E-4 22 5.10010107473039E-4 23 4.636455522482173E-5 24 4.636455522482173E-5 25 0.004265539080683599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2156820.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.35653479206184 #Duplication Level Percentage of deduplicated Percentage of total 1 84.12708754031898 58.34763273944965 2 11.002020780188037 15.261240740481977 3 2.6517019878111823 5.517385835274173 4 0.9031510847772312 2.505577185353617 5 0.3980455687100668 1.3803530667532893 6 0.22190395649033978 0.9234293687291056 7 0.12594992038243202 0.6114815024543095 8 0.0916467591126274 0.5085041309579722 9 0.06921449524064303 0.432042979254539 >10 0.3566704148281465 4.386971441493613 >50 0.02513960237655198 1.191434304247917 >100 0.02150805934510469 3.124894675702276 >500 0.0031473261761587796 1.4790148540927683 >1k 0.002544646695617524 3.216073562144802 >5k 2.6785754690710774E-4 1.1139636136100723 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6297 0.2919576042507024 No Hit CTGTAGGACGTGGAATATGGCAAGA 5994 0.27790914401758143 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5862 0.271789022727905 No Hit CTTTAGGACGTGAAATATGGCGAGG 5832 0.2703980860711603 No Hit GTATCAACGCAGAGTACTTTTTTTT 3903 0.18096085904247922 No Hit GTCCTACAGTGTGCATTTCTCATTT 3546 0.16440871282721786 No Hit CCCATGTACTCTGCGTTGATACCAC 3334 0.15457942711955563 No Hit GTACATGGGAAGCAGTGGTATCAAC 3283 0.15221483480308973 No Hit CTGAAGGACCTGGAATATGGCGAGA 3165 0.14674381728656077 No Hit TATCAACGCAGAGTACTTTTTTTTT 2821 0.1307944102892221 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2579 0.11957418792481524 No Hit ATTTAGAAATGTCCACTGTAGGACG 2547 0.11809052215762095 No Hit CTGTAGGACCTGGAATATGGCGAGA 2540 0.11776597027104718 No Hit GGTATCAACGCAGAGTACTTTTTTT 2504 0.11609684628295361 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2388 0.11071855787687429 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 6 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 7 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 8 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 9 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 10 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 11 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 12 4.636455522482173E-5 4.636455522482173E-5 0.0 0.0 0.0 13 4.636455522482173E-5 1.390936656744652E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 80 1.2702549E-7 14.267271 6 TCCAACG 205 0.0 13.424429 18 TAGGACC 985 0.0 13.233211 4 AATGGCG 85 3.9780316E-6 12.285038 16 CCAACGT 85 3.9924944E-6 12.281051 19 GAACCGT 55 0.0030413896 12.105563 6 CCAACGA 135 3.783498E-10 11.950248 19 GTCGAGG 145 1.0004442E-10 11.78057 19 AATCCCG 395 0.0 11.7722845 19 GCCGGTT 205 0.0 11.577078 11 AGGACCG 100 1.8956962E-6 11.417266 5 GGCGAGG 1710 0.0 11.099301 19 ATGTCGA 215 0.0 11.038353 17 GTATTAG 190 0.0 11.007706 1 AAATGTC 830 0.0 10.877574 7 CGGGAGT 70 0.0014730901 10.873587 4 TTCGGGA 70 0.0014825444 10.865252 2 TATGTCG 210 0.0 10.849124 16 TGTCGAG 220 0.0 10.7837305 18 ACGAAGG 80 3.7977862E-4 10.679607 16 >>END_MODULE