FastQCFastQC Report
Thu 26 May 2016
SRR1513014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561629
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44140.28265356240182526No Hit
CCCATGTACTCTGCGTTGATACCAC36500.23373029061319944No Hit
GTCCTACAGTGGACATTTCTAAATT29920.19159480260676512No Hit
GTCCTAAAGTGTGTATTTCTCATTT29590.18948162463683757No Hit
CTGTAGGACGTGGAATATGGCAAGA28630.1833341978152301No Hit
CTTTAGGACGTGAAATATGGCGAGG26650.1706551299956648No Hit
GTATCAACGCAGAGTACTTTTTTTT20120.12883982046952253No Hit
GAGTACATGGGAAGCAGTGGTATCA18050.1155844313854315No Hit
GTCCTACAGTGTGCATTTCTCATTT17480.11193439671010208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG350.00217375216.2817335
CTAGACT1459.822543E-1111.790224
GTCCTAT1800.011.6463281
TAGGACC4600.011.562394
GACGTGA9650.011.02074057
GGCGAGG8200.011.00093919
TTAGGAC11600.010.8895783
TAGGGTG1158.8425077E-710.7365055
GTCCTAA8700.010.7336051
TATGTCG909.497632E-510.55737216
GTCCTAG2350.010.5425181
TAATACT1951.4551915E-1110.2282694
TAGGACT2151.8189894E-1210.1603074
GGACGTG16550.010.1553596
ACCTTTT4500.010.135076515
AGGACGT16500.010.1308565
GTCCTAC16750.010.1261421
TCCTATA2450.010.0794912
CCTATAC1701.5643309E-910.0563643
ACGTGAA11200.010.0042388
Produced by FastQC (version 0.11.5)