FastQCFastQC Report
Thu 26 May 2016
SRR1513014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561629
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC43400.2779149208935029No Hit
GTACATGGGAAGCAGTGGTATCAAC41060.26293056801583475No Hit
CTTTAGGACGTGAAATATGGCGAGG28720.18391051907975584No Hit
GTCCTACAGTGGACATTTCTAAATT28020.17942802035566704No Hit
CTGTAGGACGTGGAATATGGCAAGA27920.17878766339508295No Hit
GTCCTAAAGTGTGTATTTCTCATTT27350.17513762871975352No Hit
GTATCAACGCAGAGTACTTTTTTTT26280.16828580924150358No Hit
TATCAACGCAGAGTACTTTTTTTTT18240.1168011096105413No Hit
GTCCTACAGTGTGCATTTCTCATTT16730.10713171950572126No Hit
GGTATCAACGCAGAGTACTTTTTTT16710.10700364811360445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT250.005981178519.02796
GGCGAGG7650.012.27984119
TAGGACC5350.012.0956044
AGGACCT9500.011.31955
GTATTAG1201.2605233E-711.0924861
CTACACT2350.010.933734
TAGAAAT5100.010.8225664
GGACCTG9600.010.6040916
CCAACGA909.626941E-510.54329919
GTCCTAC15050.010.4870581
TGGCGAG15750.010.42213518
TCCAACG1201.5473834E-610.27905818
GTAGAAC3850.010.1245883
TGGACCG856.4892747E-410.0761825
GTAATAC856.546988E-410.06648353
AGGACGT17900.09.9948725
CTAGGAC2105.456968E-129.959963
ATCCCGT1054.0988343E-59.95357310
GGACGTG17500.09.94887356
AAATGTC4700.09.9102097
Produced by FastQC (version 0.11.5)