##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513014_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1561629 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.59224374035062 31.0 31.0 34.0 27.0 34.0 2 30.709099280302812 31.0 31.0 34.0 27.0 34.0 3 30.82147808474356 31.0 31.0 34.0 27.0 34.0 4 34.32434400232065 37.0 35.0 37.0 30.0 37.0 5 34.01197915766165 37.0 35.0 37.0 30.0 37.0 6 33.894006835170195 37.0 35.0 37.0 29.0 37.0 7 33.76306856494084 37.0 35.0 37.0 29.0 37.0 8 33.64769481099544 37.0 35.0 37.0 28.0 37.0 9 35.11341105986121 39.0 35.0 39.0 28.0 39.0 10 34.996102787537886 39.0 35.0 39.0 28.0 39.0 11 35.04759132931061 39.0 35.0 39.0 28.0 39.0 12 34.903433529986955 39.0 35.0 39.0 27.0 39.0 13 34.898573220656125 39.0 35.0 39.0 27.0 39.0 14 35.957906135196005 40.0 36.0 41.0 27.0 41.0 15 35.97344759862938 40.0 36.0 41.0 27.0 41.0 16 35.9533685657733 40.0 36.0 41.0 27.0 41.0 17 35.923513203199995 40.0 36.0 41.0 27.0 41.0 18 35.84437212679836 40.0 36.0 41.0 27.0 41.0 19 35.81784726077705 40.0 36.0 41.0 27.0 41.0 20 35.805354536833015 40.0 36.0 41.0 27.0 41.0 21 35.74709806234387 40.0 36.0 41.0 27.0 41.0 22 35.71934243024432 40.0 36.0 41.0 27.0 41.0 23 35.61475997179868 40.0 36.0 41.0 27.0 41.0 24 35.605754631861984 40.0 36.0 41.0 27.0 41.0 25 35.50872518376644 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5968736792804847 1101 2 0.6334622761353899 1101 3 0.6058784197262383 1101 4 0.4140857233194737 1101 5 0.5751931188617121 1101 6 0.5722196546216907 1101 7 0.6665123463375693 1101 8 0.6658708412498981 1101 9 0.4632855434611187 1101 10 0.7880002500358287 1101 11 0.8191861896348485 1101 12 0.9510118091266193 1101 13 0.8914068818048761 1101 14 1.1067001952695321 1101 15 1.071927088860285 1101 16 1.0951536052338398 1101 17 1.021960546313693 1101 18 0.42048493402772635 1101 19 0.910703670516007 1101 20 0.8723886011317887 1101 21 1.1056856992795048 1101 22 0.9265798800605864 1101 23 0.8606686295904638 1101 24 0.762902192500384 1101 25 0.8979061404406963 1102 1 0.6147397014397598 1102 2 0.6969826378911534 1102 3 0.6636198717736725 1102 4 0.48271363291518554 1102 5 0.7174386727135982 1102 6 0.6789116164710123 1102 7 0.7155711403117664 1102 8 0.7463744925921887 1102 9 0.9957924519913135 1102 10 1.1046075769760648 1102 11 0.9229839321264208 1102 12 0.9547411160212889 1102 13 1.1149114773851494 1102 14 1.132733442841797 1102 15 1.1366342451446627 1102 16 1.051799221457408 1102 17 1.1312504620252923 1102 18 1.2000070976321169 1102 19 1.3416001768354704 1102 20 1.1494055021254042 1102 21 1.2308829930414689 1102 22 1.2385916267276045 1102 23 1.2762546202173866 1102 24 1.2726737562104589 1102 25 1.2897934902505455 1103 1 0.6566612868553783 1103 2 0.5571632402829962 1103 3 0.6851134089919348 1103 4 0.7032918910309007 1103 5 0.7349286149078154 1103 6 0.7413959383107809 1103 7 0.857038841424071 1103 8 0.797025777763217 1103 9 0.9760042980461776 1103 10 1.0570080566464028 1103 11 1.0297321127087855 1103 12 1.192278574619678 1103 13 1.1489560546819177 1103 14 1.2054055020445347 1103 15 1.0635976765400343 1103 16 1.0747783347682613 1103 17 1.1428972383952711 1103 18 1.3880413755354866 1103 19 1.3354190386949156 1103 20 1.3212982222017118 1103 21 1.0915137244886708 1103 22 1.295628836227877 1103 23 1.309540136726703 1103 24 1.2520589470146106 1103 25 1.2953607705247308 1104 1 0.6497338179477943 1104 2 0.5828945294958814 1104 3 0.6133589624886788 1104 4 0.5456521020156799 1104 5 0.6735716545269028 1104 6 0.5555136888503753 1104 7 0.7491889665476776 1104 8 0.5307889486050215 1104 9 0.7479772879734625 1104 10 0.8779754665153661 1104 11 0.8309561878622063 1104 12 0.7307672516345392 1104 13 0.8553640842437602 1104 14 0.9563960034765202 1104 15 0.9702962596324483 1104 16 1.063922690384132 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-2.7924555421154906 2216 18 -2.4329121570262657 2216 19 -2.6521886430403043 2216 20 -3.1192280382472646 2216 21 -3.0520362616169123 2216 22 -3.251056406398689 2216 23 -2.2733313011076675 2216 24 -2.6423365957564116 2216 25 -2.6162106505362033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33486.0 3 0.0 4 528.0 5 4810.0 6 5585.0 7 4419.0 8 3710.0 9 3241.0 10 2867.0 11 2623.0 12 2457.0 13 2404.0 14 2161.0 15 2315.0 16 2384.0 17 2565.0 18 2832.0 19 3319.0 20 4286.0 21 5519.0 22 7206.0 23 8855.0 24 10689.0 25 12450.0 26 14356.0 27 15999.0 28 18335.0 29 21955.0 30 27809.0 31 35783.0 32 45095.0 33 58684.0 34 75706.0 35 98709.0 36 135081.0 37 207990.0 38 675416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.63360160166319 19.518283390306706 13.21839964418878 27.629715363841324 2 13.697244158019709 20.818082155289225 39.98551511627609 25.499158570414977 3 17.101565096447363 25.12229217054755 30.130716066364034 27.645426666641054 4 11.568614891913532 17.692105684547638 37.570728582866295 33.16855084067254 5 12.561225352870768 38.322201062649135 36.212793824024686 12.903779760455405 6 31.709914887808406 37.55568244520194 18.03845268970803 12.695949977281625 7 27.389406918357036 31.585029250854017 22.771596085196112 18.253967745592835 8 24.803662740775863 37.39007292160607 20.634763998000743 17.17150033961732 9 26.38020065861128 14.779352408287739 19.98628960957421 38.85415732352677 10 14.544762188054527 29.126514211276707 35.0325151549342 21.296208445734564 11 33.82520190881637 22.135686822968296 25.07101708764623 18.96809418056911 12 23.21093701965446 24.80933484311274 32.019641649416926 19.960086487815875 13 29.13886503375901 21.823121096324133 25.664149230007638 23.37386463990922 14 20.615958039539663 22.37763133457575 27.647857467642673 29.35855315824191 15 23.128846103351748 31.085320813611162 23.12410701877638 22.661726064260705 16 21.828845637747243 27.904608366655587 28.210705522238637 22.055840473358533 17 21.753897104276167 28.63675817294912 28.00418936707888 21.60515535569584 18 21.682951100592078 27.203713593756202 29.949270434307067 21.16406487134465 19 23.49302265116842 26.970266138901465 27.507103505292484 22.029607704637627 20 23.59009958943613 27.44072888375426 27.863396415395087 21.10577511141452 21 22.648403580896762 26.797748491950667 27.213086410266264 23.34076151688631 22 23.19503953248759 28.213075209380982 26.828429580175033 21.763455677956387 23 21.763107626715435 27.906692306559368 27.788034161763132 22.54216590496206 24 23.07023380102931 27.234416413138344 28.049463796412333 21.64588598942001 25 22.572008212311037 27.16511330350842 27.76483486364259 22.49804362053795 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 132.0 1 132.0 2 239.0 3 346.0 4 346.0 5 346.0 6 781.5 7 1217.0 8 1217.0 9 1217.0 10 1744.5 11 2272.0 12 2272.0 13 2272.0 14 4068.0 15 5864.0 16 5864.0 17 5864.0 18 11308.5 19 16753.0 20 16753.0 21 16753.0 22 27737.0 23 38721.0 24 38721.0 25 38721.0 26 56940.0 27 75159.0 28 75159.0 29 75159.0 30 97047.0 31 118935.0 32 118935.0 33 118935.0 34 134687.0 35 150439.0 36 150439.0 37 150439.0 38 162131.0 39 173823.0 40 173823.0 41 173823.0 42 183059.0 43 192295.0 44 192295.0 45 192295.0 46 203325.0 47 214355.0 48 214355.0 49 214355.0 50 204978.5 51 195602.0 52 195602.0 53 195602.0 54 171245.0 55 146888.0 56 146888.0 57 146888.0 58 125346.5 59 103805.0 60 103805.0 61 103805.0 62 84429.0 63 65053.0 64 65053.0 65 65053.0 66 49894.5 67 34736.0 68 34736.0 69 34736.0 70 25311.5 71 15887.0 72 15887.0 73 15887.0 74 11001.5 75 6116.0 76 6116.0 77 6116.0 78 4209.0 79 2302.0 80 2302.0 81 2302.0 82 1494.5 83 687.0 84 687.0 85 687.0 86 439.5 87 192.0 88 192.0 89 192.0 90 117.0 91 42.0 92 42.0 93 42.0 94 24.5 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08010865576907192 2 6.40356960584108E-5 3 0.0 4 0.024269528806137693 5 0.0640997317544692 6 0.07799547779914436 7 0.03060906271592036 8 0.06717344516527293 9 0.0492434502689179 10 0.1114861468376932 11 0.002113177969927556 12 0.016201031102777933 13 0.0076842835270092965 14 0.010181675673287318 15 0.009349211624527976 16 0.02305285058102789 17 0.03419506169519137 18 9.605354408761621E-4 19 0.004866712900439221 20 0.0075562121348924744 21 5.763212645256972E-4 22 1.280713921168216E-4 23 0.0 24 1.280713921168216E-4 25 0.004546534420147167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1561629.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.21490912798028 #Duplication Level Percentage of deduplicated Percentage of total 1 83.49563209723082 59.46133852387566 2 11.378510871822126 16.206392354970966 3 2.7513967105587156 5.878214001523884 4 0.9965159103768615 2.8386716000829892 5 0.4513803085545557 1.607250382793619 6 0.24704948724972764 1.0556164070762086 7 0.15177669611216665 0.7566134534961118 8 0.09224779545453758 0.5255534696441124 9 0.06782218912328014 0.43469559317475787 >10 0.3259865653290098 3.937721497393498 >50 0.02079473141102758 1.0260267700589858 >100 0.016745517283383235 2.5851531760947837 >500 0.0023406327572560075 1.1371964774278767 >1k 0.001800486736350775 2.5495562923865034 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 4340 0.2779149208935029 No Hit GTACATGGGAAGCAGTGGTATCAAC 4106 0.26293056801583475 No Hit CTTTAGGACGTGAAATATGGCGAGG 2872 0.18391051907975584 No Hit GTCCTACAGTGGACATTTCTAAATT 2802 0.17942802035566704 No Hit CTGTAGGACGTGGAATATGGCAAGA 2792 0.17878766339508295 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2735 0.17513762871975352 No Hit GTATCAACGCAGAGTACTTTTTTTT 2628 0.16828580924150358 No Hit TATCAACGCAGAGTACTTTTTTTTT 1824 0.1168011096105413 No Hit GTCCTACAGTGTGCATTTCTCATTT 1673 0.10713171950572126 No Hit GGTATCAACGCAGAGTACTTTTTTT 1671 0.10700364811360445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.40356960584108E-5 7 0.0 0.0 0.0 0.0 6.40356960584108E-5 8 0.0 0.0 0.0 6.40356960584108E-5 6.40356960584108E-5 9 0.0 0.0 0.0 1.280713921168216E-4 6.40356960584108E-5 10 0.0 0.0 0.0 1.280713921168216E-4 6.40356960584108E-5 11 0.0 0.0 0.0 1.280713921168216E-4 6.40356960584108E-5 12 0.0 0.0 0.0 1.280713921168216E-4 6.40356960584108E-5 13 0.0 0.0 0.0 1.280713921168216E-4 6.40356960584108E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACAAT 25 0.0059811785 19.0279 6 GGCGAGG 765 0.0 12.279841 19 TAGGACC 535 0.0 12.095604 4 AGGACCT 950 0.0 11.3195 5 GTATTAG 120 1.2605233E-7 11.092486 1 CTACACT 235 0.0 10.93373 4 TAGAAAT 510 0.0 10.822566 4 GGACCTG 960 0.0 10.604091 6 CCAACGA 90 9.626941E-5 10.543299 19 GTCCTAC 1505 0.0 10.487058 1 TGGCGAG 1575 0.0 10.422135 18 TCCAACG 120 1.5473834E-6 10.279058 18 GTAGAAC 385 0.0 10.124588 3 TGGACCG 85 6.4892747E-4 10.076182 5 GTAATAC 85 6.546988E-4 10.0664835 3 AGGACGT 1790 0.0 9.994872 5 CTAGGAC 210 5.456968E-12 9.95996 3 ATCCCGT 105 4.0988343E-5 9.953573 10 GGACGTG 1750 0.0 9.9488735 6 AAATGTC 470 0.0 9.910209 7 >>END_MODULE