FastQCFastQC Report
Thu 26 May 2016
SRR1513013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165880
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC43210.1995032042403088No Hit
CCCATGTACTCTGCGTTGATACCAC34680.1601196742201784No Hit
GTATCAACGCAGAGTACTTTTTTTT31710.1464070031580697No Hit
GTCCTACAGTGGACATTTCTAAATT28480.13149389624540603No Hit
GTCCTAAAGTGTGTATTTCTCATTT26990.12461447540953331No Hit
CTGTAGGACGTGGAATATGGCAAGA26190.12092082663859494No Hit
CTTTAGGACGTGAAATATGGCGAGG25250.11658078933274235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC701.09708126E-412.20735319
TAGGACC5550.011.979334
CCAACGA1603.6379788E-1211.8682619
GGCGAGG7650.011.7906919
CCCCCCG1001.923352E-611.40353614
CCGAGTA752.069453E-411.40300918
CGAGCAA600.005899781411.07755510
ACTGCGT700.001498317210.8514818
TCCAACG2300.010.74196518
TTAGAAC2050.010.65617753
CTCTAGG3600.010.584271
AGGACGT16100.010.559535
GGACGTG15750.010.5495656
TGGCGAG14750.010.50107718
GACGTGG8050.010.4971447
CTAGTAC1002.405435E-510.4476883
CTAGGAC2850.010.3310473
TTAGGAC9950.010.1183643
TGTAGGA18350.010.0931342
GTAGGAC16850.010.0333873
Produced by FastQC (version 0.11.5)