##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513013_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2165880
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.042010176002364	31.0	30.0	34.0	23.0	34.0
2	29.01539420466508	31.0	30.0	34.0	22.0	34.0
3	29.25330073688293	31.0	30.0	34.0	23.0	34.0
4	32.68678043104881	35.0	33.0	37.0	25.0	37.0
5	32.806887269839514	35.0	35.0	37.0	26.0	37.0
6	32.80164875247013	37.0	35.0	37.0	25.0	37.0
7	32.59011394906458	36.0	33.0	37.0	25.0	37.0
8	32.60596570447116	37.0	33.0	37.0	25.0	37.0
9	33.920207952425805	38.0	34.0	39.0	23.0	39.0
10	33.79238092599775	38.0	34.0	39.0	23.0	39.0
11	33.89221702033354	38.0	34.0	39.0	23.0	39.0
12	33.78172844294236	38.0	34.0	39.0	22.0	39.0
13	33.78789729809593	38.0	34.0	39.0	22.0	39.0
14	34.77966184645502	39.0	34.0	41.0	22.0	41.0
15	34.72751260457643	39.0	34.0	41.0	19.0	41.0
16	34.61059107614457	39.0	34.0	41.0	18.0	41.0
17	34.55294152953996	39.0	34.0	41.0	18.0	41.0
18	34.23953820156241	39.0	34.0	41.0	17.0	41.0
19	34.4813678504811	39.0	34.0	41.0	18.0	41.0
20	34.56601935471956	39.0	34.0	41.0	18.0	41.0
21	34.50262202892127	39.0	34.0	41.0	18.0	41.0
22	34.420623487912536	39.0	34.0	41.0	18.0	41.0
23	34.1443057787135	39.0	34.0	41.0	10.0	41.0
24	34.14849945518681	39.0	34.0	41.0	9.0	41.0
25	34.06962897298096	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5335440544196359
1101	2	0.8657164438250504
1101	3	0.834482468383289
1101	4	0.8394090171133115
1101	5	0.6335084945039355
1101	6	0.8333611443636713
1101	7	0.8900464514530171
1101	8	0.605814327932201
1101	9	0.44140591786124617
1101	10	0.656378023942402
1101	11	0.8642645883880746
1101	12	1.0753414447761926
1101	13	0.8778875972026157
1101	14	0.8093886840410534
1101	15	0.7402503264654925
1101	16	1.1784465087993041
1101	17	1.1562425247685297
1101	18	1.2880456997974292
1101	19	0.7143456358067084
1101	20	1.021963615184447
1101	21	0.8105297093889234
1101	22	0.9414752132511595
1101	23	1.1477437806912576
1101	24	0.9147881960038404
1101	25	1.2184087263699865
1102	1	0.738931332734964
1102	2	0.7094362650208872
1102	3	0.8152810000082482
1102	4	0.8946388895080872
1102	5	0.6972571454437002
1102	6	0.7348134613862882
1102	7	0.9782023561356112
1102	8	0.893090589326853
1102	9	0.9061223444667661
1102	10	1.0422482751570215
1102	11	0.8691131762160964
1102	12	1.0103538990762004
1102	13	0.9113465316224136
1102	14	1.0266187506258149
1102	15	1.1175715503105295
1102	16	1.2163858169601411
1102	17	1.0354998601084944
1102	18	1.3934146794410864
1102	19	1.3052145986454633
1102	20	1.2451706254429027
1102	21	1.01997350445874
1102	22	1.1633723747163955
1102	23	1.31816640531828
1102	24	1.2372081766595286
1102	25	1.057599813049677
1103	1	0.49214290492294666
1103	2	0.6563403383430497
1103	3	0.6983003842686379
1103	4	0.7654102141492203
1103	5	0.48947834839339066
1103	6	0.7661604861055125
1103	7	0.5918107960758618
1103	8	0.7100060180925851
1103	9	0.9821391748888075
1103	10	0.9550460146146023
1103	11	0.8619760662810378
1103	12	1.0130423844382221
1103	13	0.9631336275109064
1103	14	1.1703525732099678
1103	15	1.2100439549461726
1103	16	1.343505689168211
1103	17	1.0976408658062198
1103	18	1.372677402947403
1103	19	1.4763912280697937
1103	20	1.323071059386315
1103	21	1.1983164279445049
1103	22	1.2938665137983065
1103	23	1.652536760064521
1103	24	1.5015230815656437
1103	25	1.4171255209920304
1104	1	0.7829458580753297
1104	2	0.6793014190976336
1104	3	0.7958403140713415
1104	4	1.0938805786430592
1104	5	0.8286121849479002
1104	6	0.9055782803114738
1104	7	0.9226709111928102
1104	8	0.9680272463141719
1104	9	1.0693763564917873
1104	10	1.1119638611938498
1104	11	1.0466886214224687
1104	12	0.9659410371274078
1104	13	1.0292540774518883
1104	14	1.0871141936297377
1104	15	1.2321534864268386
1104	16	1.1252253151986267
1104	17	1.2926371079662928
1104	18	1.435388688722071
1104	19	1.2849056598564346
1104	20	1.3850418362580186
1104	21	1.331560017880598
1104	22	1.3859686123174555
1104	23	1.5868379937994632
1104	24	1.3572187917479397
1104	25	1.1562885427322982
1105	1	0.6639684442589662
1105	2	0.8111719424511499
1105	3	0.8001163947978505
1105	4	0.892047677481564
1105	5	0.6799423290716149
1105	6	0.5747944121317516
1105	7	0.8480246899702593
1105	8	0.8788425081940261
1105	9	0.7683055023410077
1105	10	1.0942974420619507
1105	11	0.9315258634445058
1105	12	0.9427432969975982
1105	13	1.031883787874719
1105	14	1.0587950836974258
1105	15	1.1861653311538163
1105	16	1.1950464029725651
1105	17	1.146995270849196
1105	18	1.092602696114497
1105	19	1.0896689198254563
1105	20	1.2181225428937736
1105	21	1.1686866618107175
1105	22	0.8318818856232468
1105	23	1.0290818406141042
1105	24	1.195868461707903
1105	25	1.2013827365260212
1106	1	0.5494190323465311
1106	2	0.6626210585757732
1106	3	0.6811159518137408
1106	4	0.5028040815667865
1106	5	0.7255893997955951
1106	6	0.6147884649828939
1106	7	0.34283244533141044
1106	8	0.6518474228759885
1106	9	0.6939244592703915
1106	10	0.7870592735745205
1106	11	0.6133478187135779
1106	12	0.7717479430986387
1106	13	0.7978718517202452
1106	14	0.9299240304416685
1106	15	0.9636404612585991
1106	16	1.04058377408861
1106	17	0.9923846608565867
1106	18	1.2319792744633915
1106	19	0.689692551458009
1106	20	0.8677932696667554
1106	21	0.8793525506391475
1106	22	0.9037478602580435
1106	23	1.1426772670647907
1106	24	0.9702420926428417
1106	25	1.1290328802342486
1107	1	0.6622665311934419
1107	2	0.7366096604738246
1107	3	0.8342356668262525
1107	4	0.8534249147254087
1107	5	0.8571869859976715
1107	6	0.6438269816223041
1107	7	0.8957030234129562
1107	8	0.8650073911485876
1107	9	1.0714963700423326
1107	10	0.9467437292470109
1107	11	0.9129931567431271
1107	12	1.114812641828145
1107	13	1.0297811211515224
1107	14	1.2607265099306773
1107	15	1.2854775054393457
1107	16	1.208769721787327
1107	17	1.3207942203070218
1107	18	1.6279258200907378
1107	19	1.4713552172581146
1107	20	1.394088572055395
1107	21	1.4483551817767193
1107	22	1.3165932625809944
1107	23	1.3909787332338794
1107	24	1.5031513166494364
1107	25	1.4715502988541829
1108	1	0.5794227472101134
1108	2	0.5124922422413789
1108	3	0.3210771349289985
1108	4	0.5287717519345563
1108	5	0.4802947216816449
1108	6	0.6064177250371046
1108	7	0.6672906084001653
1108	8	0.7528933461571725
1108	9	0.8070890070889405
1108	10	0.7235155860908549
1108	11	0.5920654340463329
1108	12	0.666256136436651
1108	13	0.720791700098772
1108	14	0.9588693531532471
1108	15	0.8695364313195455
1108	16	0.7015350543406669
1108	17	0.8398400652951636
1108	18	1.0296824224663084
1108	19	0.9786484856086162
1108	20	0.9184605692361103
1108	21	0.8177345727632215
1108	22	0.825151665929404
1108	23	1.0088964302573729
1108	24	1.079052080810314
1108	25	1.1266392869980564
1109	1	0.14126658408807558
1109	2	0.10241787836375948
1109	3	0.35037916063083685
1109	4	0.34984156263694643
1109	5	0.3063083128024928
1109	6	0.26596526962701006
1109	7	0.24523127588837212
1109	8	0.28288078144909434
1109	9	0.4182619151007785
1109	10	0.47355914737562443
1109	11	0.048359597276594
1109	12	0.3676590990804698
1109	13	0.3666018702294096
1109	14	0.2620079947694478
1109	15	0.49923275471308415
1109	16	0.45990903069301936
1109	17	0.2891360911106702
1109	18	-1.2515580804078894
1109	19	0.1699539934906511
1109	20	0.2660601382516461
1109	21	0.36269534888919
1109	22	0.6098558865074111
1109	23	0.1924459024341303
1109	24	0.48132761342358776
1109	25	0.6272187711984145
1110	1	0.41400900626713266
1110	2	0.2691686776233908
1110	3	0.33429558573627816
1110	4	0.35579415690726535
1110	5	0.4051834788065065
1110	6	0.3151899186678122
1110	7	0.30254400236802326
1110	8	0.22565875775476485
1110	9	0.5416606227012792
1110	10	0.6513914719711167
1110	11	0.563871821418104
1110	12	0.055506274017211865
1110	13	0.2832227162039658
1110	14	0.5011183702465587
1110	15	0.6107016995470147
1110	16	0.5960556179985872
1110	17	0.2724152075580264
1110	18	0.7606146941485008
1110	19	0.5563260151883327
1110	20	0.5946502193431797
1110	21	0.4543253846003168
1110	22	0.5873323285281131
1110	23	0.42165678990841116
1110	24	0.6855486462987841
1110	25	0.6327787751439402
1111	1	0.022441026667841868
1111	2	0.3082838193787012
1111	3	0.139395694295132
1111	4	0.18777171516427416
1111	5	0.15342432937703876
1111	6	0.28922863039939983
1111	7	0.2636522705916846
1111	8	0.032816490271557086
1111	9	0.41938797158127983
1111	10	0.3007618452291041
1111	11	0.14956605266986855
1111	12	0.19361301069197623
1111	13	0.2457234500872687
1111	14	0.21864752636628992
1111	15	0.4773240509804566
1111	16	0.3301064535252607
1111	17	0.2766099684521848
1111	18	0.5663259781404122
1111	19	0.2963839917540909
1111	20	0.20673536682600258
1111	21	0.3113192731038694
1111	22	0.17655160649697166
1111	23	0.45147252352394673
1111	24	0.5875590008656388
1111	25	0.022274517589281118
1112	1	-0.07133724583806966
1112	2	0.07612742984712995
1112	3	0.10952008279715386
1112	4	0.32623582828806263
1112	5	0.19278614382769632
1112	6	0.10516613864140822
1112	7	-0.1914387370772559
1112	8	0.1058648921573564
1112	9	0.27207751458460905
1112	10	0.25090618723137936
1112	11	0.18133942021951555
1112	12	0.3058280390814261
1112	13	-0.13934164792809867
1112	14	0.15720155309278283
1112	15	0.1286181204369541
1112	16	0.31792438887492125
1112	17	0.260359455031427
1112	18	0.2596151811284031
1112	19	0.2698886025109246
1112	20	0.1766778834601439
1112	21	0.07209307647589469
1112	22	0.1019626631209789
1112	23	0.32924071086107887
1112	24	-0.012573833466959172
1112	25	0.29471050010931066
1113	1	-0.4831222600324878
1113	2	-0.24836247276779844
1113	3	-0.4996655849388887
1113	4	-0.0730670427450093
1113	5	-0.383573403256662
1113	6	-0.16988687066861274
1113	7	-0.28945831580062986
1113	8	-0.04498743175552278
1113	9	-0.27665290960819533
1113	10	-0.5778174529781737
1113	11	-0.4184521426502812
1113	12	-0.21977625278660895
1113	13	-0.3754269505592873
1113	14	-0.3337121136711474
1113	15	-0.7996141509311059
1113	16	-0.7250107395789271
1113	17	-0.6438409500774398
1113	18	-0.7705356970424546
1113	19	-0.6105821273790397
1113	20	-0.8736198473041696
1113	21	-0.6873726739334387
1113	22	-0.5616100070268004
1113	23	-0.9773579884167134
1113	24	-0.3900816058020524
1113	25	-0.46429453693951217
1114	1	-1.3474253416114195
1114	2	-1.1483417936872513
1114	3	-1.033949265135547
1114	4	-1.1318267284856738
1114	5	-1.2377564381125836
1114	6	-1.5947376319631452
1114	7	-1.879179418765208
1114	8	-1.289312706846271
1114	9	-1.4845234485007452
1114	10	-1.6412775671395679
1114	11	-2.2702915208822105
1114	12	-1.8843762486788407
1114	13	-1.6332467485806816
1114	14	-1.9739422214391027
1114	15	-1.8571811808055756
1114	16	-2.569615079150381
1114	17	-4.699826158142233
1114	18	-3.586521904829848
1114	19	-2.520850177589203
1114	20	-1.7440952261243865
1114	21	-2.9155274359413106
1114	22	-3.0478584184618143
1114	23	-2.8107640325976853
1114	24	-2.453399432711965
1114	25	-2.2558754703818273
1115	1	-1.2143178686185507
1115	2	-0.956528211744125
1115	3	-0.8952927553339478
1115	4	-0.9294871104971705
1115	5	-0.882317826591624
1115	6	-1.0060011186093156
1115	7	-2.070688123087315
1115	8	-1.5103087058845936
1115	9	-1.2148280776968434
1115	10	-1.024782581897739
1115	11	-1.8122127302378104
1115	12	-1.3574717064892354
1115	13	-1.2882140047737067
1115	14	-1.375789495908272
1115	15	-2.674414392460214
1115	16	-1.4655891676888544
1115	17	-1.5166168268071942
1115	18	-1.4667116849589448
1115	19	-1.6068898775414269
1115	20	-1.3475769549670247
1115	21	-2.036295618602452
1115	22	-1.994870786968896
1115	23	-1.452766892042746
1115	24	-1.3389173082877264
1115	25	-1.755348289601038
1116	1	-1.4048321070531244
1116	2	-2.155159469477031
1116	3	-1.8137675543940226
1116	4	-1.4951420523323193
1116	5	-2.0312136019331497
1116	6	-1.7149587061627933
1116	7	-1.3224070250441962
1116	8	-2.030518402992314
1116	9	-2.064367619937528
1116	10	-2.945968582782548
1116	11	-1.7694079568639793
1116	12	-1.6432292689082786
1116	13	-2.244448987506537
1116	14	-2.014390758180049
1116	15	-1.6353655107671798
1116	16	-1.474024115271753
1116	17	-1.9202793734039787
1116	18	-1.73237126773369
1116	19	-2.2061397996342436
1116	20	-2.4867328972356475
1116	21	-2.9815807748362566
1116	22	-2.112415898975023
1116	23	-2.035143729320559
1116	24	-2.6066860465996555
1116	25	-2.4312619885383384
1201	1	0.26682783297639645
1201	2	0.34383406122937643
1201	3	0.48780372337061806
1201	4	0.6016619024048566
1201	5	0.6730156238063287
1201	6	0.6935182713573695
1201	7	0.40613308248768476
1201	8	-0.010268934689698028
1201	9	0.3507600379599225
1201	10	0.6011774974102835
1201	11	0.2759983596402975
1201	12	-0.36868159062544237
1201	13	0.0739803140886579
1201	14	0.6515749504545028
1201	15	0.30840542786985736
1201	16	0.7714413835132703
1201	17	0.8791627569694995
1201	18	1.0562353179708097
1201	19	0.5931679327311556
1201	20	0.5731894146526102
1201	21	0.47972489339131386
1201	22	0.7233655664954668
1201	23	1.0095094193990874
1201	24	0.7393041096725526
1201	25	0.6792736363652381
1202	1	0.7535000414687865
1202	2	0.880796280085093
1202	3	0.5989864616125296
1202	4	0.8045496950275677
1202	5	0.7474724472400567
1202	6	0.7807805102886434
1202	7	0.8348671120853766
1202	8	0.8699274390254317
1202	9	1.1999166682529747
1202	10	1.2832082304074817
1202	11	0.8867285092115935
1202	12	1.0851017905954734
1202	13	1.0694166337026942
1202	14	1.204291146674045
1202	15	0.9053791457333062
1202	16	1.1269003905610475
1202	17	1.3921639699221018
1202	18	1.5851387829964096
1202	19	1.3061232506854523
1202	20	1.2374868666289132
1202	21	1.2647397554752544
1202	22	1.2580776581717075
1202	23	1.3791979339933391
1202	24	1.535810214942174
1202	25	1.4705814267839443
1203	1	0.22593507176138417
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2211	15	0.25552133613000194
2211	16	0.1669158813618239
2211	17	0.37884506404716234
2211	18	0.3706317005122344
2211	19	0.08916469382191394
2211	20	-0.07439235865382443
2211	21	0.006717736873454783
2211	22	-0.11780754497596746
2211	23	0.45807524336095895
2211	24	0.21920038193665903
2211	25	0.37755271210899366
2212	1	-0.3668282777014724
2212	2	-0.34389207390780996
2212	3	-0.279138628001963
2212	4	-0.49241918444877797
2212	5	-0.35211779548112787
2212	6	-0.513925393097999
2212	7	-0.4671145143331046
2212	8	-0.38495077554591006
2212	9	-0.2765419370216122
2212	10	-0.29195668680731757
2212	11	-0.5686432975475242
2212	12	-0.26309728120197207
2212	13	-0.4436189334698497
2212	14	-0.4508724712412757
2212	15	-0.45572857256582466
2212	16	-0.31854275021752443
2212	17	-0.2738809270703584
2212	18	-3.152182426138058
2212	19	-1.435572650219413
2212	20	-0.7011577870015628
2212	21	-0.7577036072817265
2212	22	-0.49986081380254177
2212	23	-0.5449006725699306
2212	24	-0.9001432602236861
2212	25	-0.598007689759676
2213	1	-0.1877213045291768
2213	2	-0.05888609618263274
2213	3	-0.4811277335169031
2213	4	-0.5020056907690815
2213	5	-0.38452544737820205
2213	6	-0.4142123716057853
2213	7	-0.30872915769850806
2213	8	-0.5108289083151192
2213	9	-0.5078066492271063
2213	10	-0.580336782907473
2213	11	-0.6322147609198652
2213	12	-0.623713475811762
2213	13	-0.4563042659482335
2213	14	-0.5539774131113475
2213	15	-0.6771945172491272
2213	16	-1.2258560685347106
2213	17	-0.8508682864558281
2213	18	-0.6625599716967017
2213	19	-0.5913630029307555
2213	20	-1.3475838603090224
2213	21	-0.7814276295599569
2213	22	-0.7565264288165992
2213	23	-1.213504038153559
2213	24	-0.9479641247162007
2213	25	-1.0259263579449964
2214	1	-2.022468983454921
2214	2	-4.091988950441358
2214	3	-2.333186132861428
2214	4	-1.8894645323711288
2214	5	-2.018232254204147
2214	6	-2.034629446906841
2214	7	-2.1141110151005513
2214	8	-1.6931300879660434
2214	9	-1.980440584312415
2214	10	-2.1161791114999815
2214	11	-1.8482617394469258
2214	12	-2.542445452925236
2214	13	-2.335318173613608
2214	14	-3.4165925653760993
2214	15	-3.7390593111661516
2214	16	-3.037848103621897
2214	17	-3.3717182954747287
2214	18	-2.6680051602482777
2214	19	-4.811200305684846
2214	20	-3.1441458432191567
2214	21	-2.9296283523622684
2214	22	-3.142799866136393
2214	23	-3.3105377700772074
2214	24	-3.612843537912358
2214	25	-3.553970630006635
2215	1	-1.204980630029496
2215	2	-2.8487464943538185
2215	3	-1.6471095001100124
2215	4	-1.3413573976482418
2215	5	-1.207677991355844
2215	6	-1.7749135642778526
2215	7	-2.0475773633483385
2215	8	-2.0661015994212626
2215	9	-1.7505917888832272
2215	10	-3.48689576264972
2215	11	-2.4771371640535307
2215	12	-2.594099282054348
2215	13	-1.9850825338744684
2215	14	-3.3662442891756896
2215	15	-3.116051105175952
2215	16	-2.780967889412832
2215	17	-3.103141381909115
2215	18	-2.057585155444471
2215	19	-2.5978078881565345
2215	20	-2.9304517890657458
2215	21	-2.6295773396330624
2215	22	-2.1768071091447823
2215	23	-2.7406121048139447
2215	24	-2.56161281697241
2215	25	-2.436189652648917
2216	1	-1.9200970499484562
2216	2	-1.958011849759977
2216	3	-2.43591594715274
2216	4	-3.0924817051617026
2216	5	-2.269983139564978
2216	6	-2.3110053870406766
2216	7	-2.110090016756871
2216	8	-2.687989460153087
2216	9	-2.9419673112309113
2216	10	-2.3909151058324163
2216	11	-2.8473133423025523
2216	12	-3.491001984458592
2216	13	-3.9514918592814006
2216	14	-3.378336860121454
2216	15	-3.206162977358229
2216	16	-2.326865274496342
2216	17	-2.4589670004976085
2216	18	-2.4378784962310966
2216	19	-2.7212746802622654
2216	20	-2.5615363932328883
2216	21	-2.9533825261501896
2216	22	-3.313565961822512
2216	23	-3.4015828507754478
2216	24	-3.298513944254676
2216	25	-3.419380019408365
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107034.0
3	4.0
4	957.0
5	5397.0
6	6622.0
7	6023.0
8	5359.0
9	4954.0
10	4378.0
11	4286.0
12	4162.0
13	4053.0
14	3976.0
15	4056.0
16	4245.0
17	4625.0
18	5228.0
19	6062.0
20	7961.0
21	10208.0
22	12522.0
23	15256.0
24	18066.0
25	20175.0
26	22139.0
27	24813.0
28	28460.0
29	33749.0
30	41246.0
31	51230.0
32	64863.0
33	82289.0
34	107050.0
35	140455.0
36	192349.0
37	295532.0
38	816096.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.77832331533615	18.2353033849895	12.272046544300677	25.71432675537367
2	14.945739656585951	20.234500756342307	40.57158693317629	24.248172653895452
3	18.698989100277537	24.758429283437586	29.702217125205703	26.84036449107917
4	12.327087946245886	16.870581649591383	38.11224145750948	32.690088946653255
5	13.14092378752887	38.03150115473441	35.74988452655889	13.07769053117783
6	31.92100792294375	36.823101358911615	18.33267682082935	12.923213897315286
7	27.793322081094622	31.51902913652537	22.58970812677933	18.097940655600684
8	25.092306306847178	36.52808213009936	20.892836938224622	17.48677462482884
9	26.696921179251753	15.037034608274563	19.87690997581517	38.38913423665851
10	14.920641371224006	28.841478085344065	34.7098927091843	21.52798783424763
11	34.21473596712	22.40864485441825	24.671084532083402	18.705534646378354
12	23.357496366770743	24.646525007284932	31.74650381056133	20.249474815383003
13	29.00117575017572	21.830254185550558	25.597272776811586	23.57129728746214
14	21.23520740119486	21.939691641512013	27.51224788386863	29.312853073424495
15	23.415269681488304	30.24480223603838	23.25103982989556	23.088888252577753
16	22.433556125222186	27.780469095995702	27.42713929395922	22.35883548482289
17	22.18728843068562	28.136444370143433	27.566425260210053	22.109841938960898
18	22.05013126756397	27.293762017453037	29.103174456441355	21.552932258541635
19	23.583409957775352	26.92305027933477	27.32215932680713	22.171380436082746
20	23.743242506837156	27.267437403423152	27.523328533546632	21.465991556193064
21	23.163576191665946	26.795759866143815	27.053921634279448	22.986742307910788
22	23.434093502518426	27.607011788371782	26.73753451378681	22.22136019532298
23	22.62979853374127	27.45635282572303	27.23089534145356	22.682953299082147
24	23.20861966494987	27.055688218753737	27.59501761052004	22.140674505776353
25	23.028891002964837	26.98833461101516	27.293500447958323	22.689273938061678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	284.0
1	284.0
2	465.0
3	646.0
4	646.0
5	646.0
6	1238.0
7	1830.0
8	1830.0
9	1830.0
10	2748.0
11	3666.0
12	3666.0
13	3666.0
14	6305.5
15	8945.0
16	8945.0
17	8945.0
18	15984.5
19	23024.0
20	23024.0
21	23024.0
22	37013.0
23	51002.0
24	51002.0
25	51002.0
26	73447.5
27	95893.0
28	95893.0
29	95893.0
30	124945.5
31	153998.0
32	153998.0
33	153998.0
34	176078.5
35	198159.0
36	198159.0
37	198159.0
38	216270.0
39	234381.0
40	234381.0
41	234381.0
42	249598.5
43	264816.0
44	264816.0
45	264816.0
46	277994.0
47	291172.0
48	291172.0
49	291172.0
50	281402.5
51	271633.0
52	271633.0
53	271633.0
54	243942.0
55	216251.0
56	216251.0
57	216251.0
58	186086.5
59	155922.0
60	155922.0
61	155922.0
62	127462.0
63	99002.0
64	99002.0
65	99002.0
66	76719.0
67	54436.0
68	54436.0
69	54436.0
70	39789.5
71	25143.0
72	25143.0
73	25143.0
74	17540.5
75	9938.0
76	9938.0
77	9938.0
78	6911.5
79	3885.0
80	3885.0
81	3885.0
82	2615.5
83	1346.0
84	1346.0
85	1346.0
86	861.5
87	377.0
88	377.0
89	377.0
90	237.0
91	97.0
92	97.0
93	97.0
94	58.5
95	20.0
96	20.0
97	20.0
98	17.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35846861321956897
2	0.03947587123940385
3	0.03596690490701239
4	0.035182004543187984
5	0.040630136480322085
6	0.018329732025781666
7	0.01777568471014091
8	0.02470127615565036
9	0.021838698358173123
10	0.02109996860398545
11	0.019853362143793747
12	0.02082294494616507
13	0.020961456775075257
14	0.028071730659131624
15	0.02871811919404584
16	0.02220806323526696
17	0.02373169335327904
18	0.10896263874268196
19	0.025717029567658412
20	0.0233623284761852
21	0.026363418102572627
22	0.020361238849797774
23	0.023316157866548472
24	0.01906846177996934
25	0.02308530481836482
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2165880.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.16802586778287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.61239288128593	56.449681019444434
2	12.157374713170071	16.81803217289754
3	3.24846253698525	6.74069222366158
4	1.212327719211713	3.354172601706639
5	0.5660119244287327	1.9574963715180076
6	0.30935019190674135	1.283828524560545
7	0.18978484234425358	0.91889300192063
8	0.12919519632623108	0.7148941345188832
9	0.1031927869330745	0.6423877220359953
>10	0.4310629924190318	5.142832663356235
>50	0.022403376813352024	1.0669999442599865
>100	0.014956781397966308	2.093388210358318
>500	0.0022110360319612084	1.0707626988357926
>1k	0.0012730207456743447	1.7459387109254672
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4321	0.1995032042403088	No Hit
CCCATGTACTCTGCGTTGATACCAC	3468	0.1601196742201784	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3171	0.1464070031580697	No Hit
GTCCTACAGTGGACATTTCTAAATT	2848	0.13149389624540603	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2699	0.12461447540953331	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2619	0.12092082663859494	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2525	0.11658078933274235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.8468243854691857E-4	0.0	0.0	0.0	0.0
7	1.8468243854691857E-4	0.0	0.0	0.0	0.0
8	1.8468243854691857E-4	0.0	0.0	0.0	0.0
9	1.8468243854691857E-4	0.0	0.0	4.617060963672964E-5	0.0
10	1.8468243854691857E-4	0.0	0.0	4.617060963672964E-5	0.0
11	2.3085304818364823E-4	0.0	0.0	4.617060963672964E-5	0.0
12	2.3085304818364823E-4	0.0	0.0	4.617060963672964E-5	0.0
13	2.3085304818364823E-4	0.0	0.0	4.617060963672964E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAC	70	1.09708126E-4	12.207353	19
TAGGACC	555	0.0	11.97933	4
CCAACGA	160	3.6379788E-12	11.86826	19
GGCGAGG	765	0.0	11.79069	19
CCCCCCG	100	1.923352E-6	11.403536	14
CCGAGTA	75	2.069453E-4	11.403009	18
CGAGCAA	60	0.0058997814	11.077555	10
ACTGCGT	70	0.0014983172	10.851481	8
TCCAACG	230	0.0	10.741965	18
TTAGAAC	205	0.0	10.6561775	3
CTCTAGG	360	0.0	10.58427	1
AGGACGT	1610	0.0	10.55953	5
GGACGTG	1575	0.0	10.549565	6
TGGCGAG	1475	0.0	10.501077	18
GACGTGG	805	0.0	10.497144	7
CTAGTAC	100	2.405435E-5	10.447688	3
CTAGGAC	285	0.0	10.331047	3
TTAGGAC	995	0.0	10.118364	3
TGTAGGA	1835	0.0	10.093134	2
GTAGGAC	1685	0.0	10.033387	3
>>END_MODULE