FastQCFastQC Report
Thu 26 May 2016
SRR1513013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165880
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC42410.1958095554693704No Hit
GTACATGGGAAGCAGTGGTATCAAC40180.1855135095203797No Hit
GTATCAACGCAGAGTACTTTTTTTT36720.16953847858607124No Hit
TATCAACGCAGAGTACTTTTTTTTT27330.1261842761371821No Hit
GTCCTACAGTGGACATTTCTAAATT27250.12581491126008826No Hit
CTTTAGGACGTGAAATATGGCGAGG26410.121936580050603No Hit
GTCCTAAAGTGTGTATTTCTCATTT26280.1213363621253255No Hit
CTGTAGGACGTGGAATATGGCAAGA24610.11362587031599165No Hit
GGTATCAACGCAGAGTACTTTTTTT23380.10794688533067391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT250.006006174719.0145681
GGCGAGG7300.012.22077719
TAGGACC4800.011.8920824
TGGCGAG15200.011.051558518
TCCAACG1408.485586E-910.84640518
GTATTAA2550.010.8122061
CCAACGA803.8066102E-410.67692919
AGGACGT14350.010.6075725
CGGTTTC1002.4191248E-510.44231613
GGACGTG14650.010.2576246
TAGGACG15400.010.1932134
CTAGGAC2850.010.0074373
GACGTGA8650.09.9979537
ATGGCGA17300.09.98687117
ACTATAC2105.456968E-129.9597833
GTCCTAA8900.09.9345791
ATTATAC2500.09.8873473
TTAGGAC10600.09.8658223
AGGACCT11200.09.853445
GTAGGAC14200.09.8418213
Produced by FastQC (version 0.11.5)