##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513013_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2165880
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.395742146379302	31.0	31.0	34.0	27.0	34.0
2	30.547069089700262	31.0	31.0	34.0	27.0	34.0
3	30.66566707296803	31.0	31.0	34.0	27.0	34.0
4	34.144364877093835	37.0	35.0	37.0	30.0	37.0
5	33.778618852383325	37.0	35.0	37.0	28.0	37.0
6	33.636198219661296	37.0	35.0	37.0	28.0	37.0
7	33.51403540362347	37.0	35.0	37.0	28.0	37.0
8	33.399709586865384	37.0	35.0	37.0	27.0	37.0
9	34.85284226272924	39.0	35.0	39.0	27.0	39.0
10	34.74067030491071	39.0	35.0	39.0	27.0	39.0
11	34.770630875210074	39.0	35.0	39.0	27.0	39.0
12	34.60741684673204	39.0	35.0	39.0	27.0	39.0
13	34.59278861248084	39.0	35.0	39.0	27.0	39.0
14	35.650321347443075	40.0	36.0	41.0	27.0	41.0
15	35.66365449609397	40.0	36.0	41.0	27.0	41.0
16	35.63280052449812	40.0	36.0	41.0	27.0	41.0
17	35.614260716198494	40.0	36.0	41.0	27.0	41.0
18	35.53560261879698	40.0	36.0	41.0	27.0	41.0
19	35.504995659962695	40.0	36.0	41.0	26.0	41.0
20	35.4839981901121	40.0	36.0	41.0	26.0	41.0
21	35.42928555598648	40.0	36.0	41.0	26.0	41.0
22	35.38868450699023	40.0	36.0	41.0	25.0	41.0
23	35.28433892921122	40.0	36.0	41.0	25.0	41.0
24	35.26827848264909	40.0	36.0	41.0	25.0	41.0
25	35.17374092747521	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39615765293063276
1101	2	0.5014971513810238
1101	3	0.6125253431660518
1101	4	0.3630953854289416
1101	5	0.5947259520683872
1101	6	0.5789895505318015
1101	7	0.5254087469035014
1101	8	0.5464063963718218
1101	9	0.4320015017662584
1101	10	0.8138303036321801
1101	11	0.7875398364516002
1101	12	0.9219424992881713
1101	13	0.9692936785759727
1101	14	0.9931407470178826
1101	15	0.8393118691545354
1101	16	1.0791426890503075
1101	17	0.9670160690966156
1101	18	0.36303156487345944
1101	19	0.7234784945800001
1101	20	0.5447478024536707
1101	21	0.880288886490284
1101	22	0.784803253888775
1101	23	0.705587444217592
1101	24	0.683164613555256
1101	25	0.7788370564392437
1102	1	0.5778188362198371
1102	2	0.5252531217894578
1102	3	0.5082427071116911
1102	4	0.40821038697873036
1102	5	0.7598010699827853
1102	6	0.6660309167330354
1102	7	0.7094237368765803
1102	8	0.6495593695325255
1102	9	0.7813935377362142
1102	10	0.8267603690966112
1102	11	0.9264084557234611
1102	12	0.879312942825571
1102	13	0.9492499111408819
1102	14	0.9305912192356445
1102	15	1.0313482125571198
1102	16	0.9348815632712117
1102	17	1.0116902220361652
1102	18	1.1147960796412306
1102	19	1.0416684945426624
1102	20	1.0056676281308157
1102	21	1.1377495163380331
1102	22	0.9856733743986013
1102	23	1.0345439718019094
1102	24	0.9850193146333837
1102	25	1.0722928539438996
1103	1	0.5303695379571565
1103	2	0.5267348753463281
1103	3	0.5066283976163035
1103	4	0.5730073300096734
1103	5	0.5741270212171088
1103	6	0.5712600575832667
1103	7	0.583910900861369
1103	8	0.6882106992286054
1103	9	0.7882711726037002
1103	10	0.7728240123120287
1103	11	0.7981904057504181
1103	12	0.8831301538231671
1103	13	0.8511449701137295
1103	14	1.0378887054913761
1103	15	0.8129058448425823
1103	16	0.8791041517850289
1103	17	0.9544656051801041
1103	18	1.0171483565940491
1103	19	1.1580506993170374
1103	20	1.1174925633756985
1103	21	0.7472803610503007
1103	22	0.9327872824986017
1103	23	1.06881186389883
1103	24	0.9530611506695053
1103	25	1.0183571335732893
1104	1	0.5162496084223882
1104	2	0.5264294468927879
1104	3	0.471873031241973
1104	4	0.5247314722812533
1104	5	0.4389440460166938
1104	6	0.6142929216668804
1104	7	0.6775253962062564
1104	8	0.6586425815355241
1104	9	0.803746783687636
1104	10	0.7766919035816571
1104	11	0.7418220876272201
1104	12	0.7487558402392267
1104	13	0.9044543283174704
1104	14	1.004924910316717
1104	15	0.90697259368212
1104	16	0.830634625263869
1104	17	0.947388692145573
1104	18	0.9271220386774601
1104	19	0.930921981229119
1104	20	0.9001308124587553
1104	21	0.8576692352187223
1104	22	0.952959856318202
1104	23	0.7794652928749386
1104	24	0.9291187787338941
1104	25	1.0065060240552057
1105	1	0.5346259793362123
1105	2	0.5327168534074147
1105	3	0.4333136328328777
1105	4	0.4086585578696571
1105	5	0.38669212010834997
1105	6	0.45797738407887323
1105	7	0.6029495573798442
1105	8	0.7572505945713246
1105	9	0.7276172797285838
1105	10	0.7578216303307599
1105	11	0.7863993559866245
1105	12	0.730752303436276
1105	13	0.8324954190310123
1105	14	0.9340982471890129
1105	15	0.8785827511924964
1105	16	0.9855052227640186
1105	17	0.6517043673469232
1105	18	0.9705785286565956
1105	19	1.0057655182736767
1105	20	1.0757663177864174
1105	21	0.9922315350633113
1105	22	1.035026080375303
1105	23	1.078580059162924
1105	24	1.1364022887425094
1105	25	1.103180972325461
1106	1	0.3520015077778851
1106	2	0.3641998560722932
1106	3	0.35553626199587995
1106	4	0.22773905191902344
1106	5	0.3785798428212246
1106	6	0.3267182279112788
1106	7	0.41066096892951975
1106	8	0.5081426668998148
1106	9	0.45808124454099186
1106	10	0.48693606324133754
1106	11	0.37108727891436644
1106	12	0.47803614811977013
1106	13	0.45462683532445425
1106	14	0.5428961034249724
1106	15	0.48189309818508264
1106	16	0.6452200037833649
1106	17	0.5728882510967281
1106	18	0.6556804847627973
1106	19	0.5827606035917157
1106	20	0.7236326691321153
1106	21	0.6934168663596125
1106	22	0.6888779492799131
1106	23	0.8012475036678239
1106	24	0.7188597692442329
1106	25	0.7587759139352741
1107	1	0.5755976229189841
1107	2	0.5501930668288608
1107	3	0.5324159704392812
1107	4	0.6385994828245813
1107	5	0.6769738181101985
1107	6	0.6688850902535179
1107	7	0.6480867170391562
1107	8	0.7510590365589209
1107	9	0.763554132991942
1107	10	0.9573882659891808
1107	11	0.8942201496147462
1107	12	0.818226163074975
1107	13	0.912780566438272
1107	14	1.0915563372572734
1107	15	1.080573860481195
1107	16	0.993047131405767
1107	17	0.8954980390682721
1107	18	1.0322310389439693
1107	19	1.1352448971330276
1107	20	1.1504293901686324
1107	21	1.017943838140404
1107	22	1.1643140262642717
1107	23	1.16903187967241
1107	24	1.183477083736804
1107	25	1.1162937868020606
1108	1	0.3905279968190385
1108	2	0.3199657199156647
1108	3	0.25933694580595557
1108	4	0.30889841612700053
1108	5	0.45894928256475964
1108	6	0.461299996186348
1108	7	0.4953915037322929
1108	8	0.554637796300895
1108	9	0.4524092841937204
1108	10	0.670162508254478
1108	11	0.6131073735746
1108	12	0.5873869413119408
1108	13	0.6249681212176057
1108	14	0.5955609160995365
1108	15	0.7140436946270725
1108	16	0.6455565773032674
1108	17	0.5405109791824785
1108	18	0.6771948764194917
1108	19	0.5720498435581476
1108	20	0.605955078014766
1108	21	0.710574634552124
1108	22	0.832520373022021
1108	23	0.7112426365086009
1108	24	0.7136716870769533
1108	25	0.7251391140838663
1109	1	0.2863454838842294
1109	2	0.2498941602957494
1109	3	0.3089962148816312
1109	4	0.40178519864626594
1109	5	0.4811574551382378
1109	6	0.16221587219867928
1109	7	0.26467409361402616
1109	8	0.481209720576409
1109	9	0.5620512512715976
1109	10	0.45519599088731155
1109	11	0.4052093553067664
1109	12	0.4918633397738148
1109	13	0.4874343399517471
1109	14	0.7488221929420504
1109	15	0.7234062228695848
1109	16	0.5471866031439632
1109	17	0.5979786936341682
1109	18	0.5063977151178349
1109	19	0.738064111920842
1109	20	0.6657452385415041
1109	21	0.8180182882004345
1109	22	0.7178994872237112
1109	23	0.7411673673730235
1109	24	0.3692509858015214
1109	25	0.5718932368361607
1110	1	0.1983739360595429
1110	2	0.23181008982096785
1110	3	0.2927561499510638
1110	4	0.34010201434537635
1110	5	0.2576662187509058
1110	6	0.38421243248966164
1110	7	0.380128756044364
1110	8	0.44147720761264964
1110	9	0.44863531099515797
1110	10	0.5128156392096557
1110	11	0.37515976997975997
1110	12	0.5995395770775573
1110	13	0.21962007399923777
1110	14	0.5832111087374372
1110	15	0.6545423302917186
1110	16	0.7141625808141825
1110	17	0.5389355320780922
1110	18	0.49671086243110807
1110	19	0.5604271012252511
1110	20	0.5966622257823602
1110	21	0.5855441483068731
1110	22	0.448317242238474
1110	23	0.6567489283756416
1110	24	0.5753772038042584
1110	25	0.5843138015040878
1111	1	-0.3232403132163064
1111	2	-0.18671648569431554
1111	3	-0.1731854328523319
1111	4	-0.2597303121244252
1111	5	0.011114610658943036
1111	6	-0.22907753002404974
1111	7	-0.06614681737099914
1111	8	0.0537019540973418
1111	9	-0.3521637901385546
1111	10	-0.2948438416900814
1111	11	-0.09214763089055111
1111	12	-0.16210913871047694
1111	13	-0.018966912468343367
1111	14	0.12965386571113413
1111	15	0.17264563028662394
1111	16	-5.917872635166077E-4
1111	17	0.12967389475199553
1111	18	0.15579084483682237
1111	19	0.13776009152097402
1111	20	0.1121470989325104
1111	21	0.08811190174547079
1111	22	-0.08974751158375938
1111	23	-0.006206215731531017
1111	24	0.09804179803320068
1111	25	0.26121345896218173
1112	1	-0.07303960553972644
1112	2	-0.32410612009749684
1112	3	-0.1329411489511223
1112	4	-0.3149802708231064
1112	5	-0.06439339990766513
1112	6	0.06129323515136065
1112	7	-0.002962795085210246
1112	8	0.09926167314696954
1112	9	-0.21501590700425055
1112	10	0.0501789472759242
1112	11	-0.11667129541023513
1112	12	0.04019638145724258
1112	13	-0.020350410134980734
1112	14	0.03544062992367003
1112	15	-0.02620286391826454
1112	16	-0.2921186458380802
1112	17	-0.11536782022840697
1112	18	-0.06861464162832931
1112	19	-0.059368652541664346
1112	20	0.13730297652526957
1112	21	0.29302926344703195
1112	22	0.17242538215401737
1112	23	0.2829060511007029
1112	24	0.2751534683050849
1112	25	0.26125538313389995
1113	1	-0.24613490195842047
1113	2	-0.6180884903007389
1113	3	-0.14682657969251878
1113	4	-0.2069857862921225
1113	5	-0.18104719765013755
1113	6	-0.2793633426479758
1113	7	-0.08002772033928807
1113	8	0.022757995789987717
1113	9	-0.07440148993210727
1113	10	-0.25594703827513143
1113	11	-0.1281725974499679
1113	12	-0.10228598073177153
1113	13	-0.011327477435131073
1113	14	-0.09919978665519835
1113	15	0.050949168889225405
1113	16	-0.21745144410613193
1113	17	-0.10232786983439723
1113	18	0.059156530353448034
1113	19	-0.08605218014246674
1113	20	0.13534666507531767
1113	21	-0.18611623998637583
1113	22	-0.34594529036077404
1113	23	-0.008275702166230303
1113	24	-0.2710441233472878
1113	25	-0.28384035671091823
1114	1	-0.5818480205048182
1114	2	-0.380754344651546
1114	3	-0.4803483095953496
1114	4	-0.23575140190554578
1114	5	-0.3387550052939403
1114	6	-0.8179622446193235
1114	7	-0.5746873292283396
1114	8	-0.906310283366544
1114	9	-0.7375922233944294
1114	10	-0.9358093009162829
1114	11	-0.6946018959870202
1114	12	-0.7471082136385974
1114	13	-0.7055608349614246
1114	14	-1.8494661937569248
1114	15	-0.9862227775418049
1114	16	-1.0573607168054622
1114	17	-1.1688907534171165
1114	18	-1.0871598836646754
1114	19	-1.1451797506029067
1114	20	-0.43897458555456126
1114	21	-0.5231011178622538
1114	22	-0.6343165121083985
1114	23	-0.7068329424303386
1114	24	-1.4658551671146043
1114	25	-1.342285195820331
1115	1	-1.3930464875002464
1115	2	-1.2226466591357017
1115	3	-0.8841262005536485
1115	4	-2.0933572113180787
1115	5	-1.3549542906059955
1115	6	-1.221168840804289
1115	7	-1.0119225266316505
1115	8	-0.8703029488066036
1115	9	-1.439857685307551
1115	10	-1.4087603256360453
1115	11	-1.1588232196428407
1115	12	-1.748267887556544
1115	13	-1.2730972116732957
1115	14	-1.9533352921486227
1115	15	-1.9153001876534574
1115	16	-1.4358260896322506
1115	17	-1.4656137646704366
1115	18	-1.396861487375432
1115	19	-1.2310618121533565
1115	20	-1.2565800219170526
1115	21	-1.469528656152086
1115	22	-1.6161493878364368
1115	23	-1.8625669732682084
1115	24	-1.314830810495927
1115	25	-1.1627083154479863
1116	1	-0.8112970542211713
1116	2	-0.8284284246129161
1116	3	-0.6813311747477933
1116	4	-0.8145788405845096
1116	5	-0.7955921279472804
1116	6	-1.5359467885903726
1116	7	-0.8021867444132411
1116	8	-0.8776914522263866
1116	9	-1.0803410798343052
1116	10	-1.0954945905383227
1116	11	-0.952692777662719
1116	12	-1.5242544380817051
1116	13	-1.163403181962984
1116	14	-1.5523115423396092
1116	15	-1.442739543650525
1116	16	-1.7480870054889905
1116	17	-1.6202026651377466
1116	18	-2.198938012801243
1116	19	-1.529318014984426
1116	20	-1.435651473567873
1116	21	-1.4532307448300799
1116	22	-2.6878350791657866
1116	23	-2.5239027792176074
1116	24	-1.8953905843625591
1116	25	-2.2745053606207577
1201	1	0.6858704824957584
1201	2	0.664151559043745
1201	3	0.536718646155915
1201	4	0.5707877426139163
1201	5	0.7229593444687907
1201	6	0.7383762193381003
1201	7	0.8418247632898854
1201	8	0.7202577486949338
1201	9	0.9698717338536795
1201	10	0.9343759562224108
1201	11	0.9208033288370956
1201	12	0.8604443655402392
1201	13	0.8839411268708375
1201	14	0.9159607594772226
1201	15	0.9039963699898266
1201	16	0.8564588134529814
1201	17	0.9007317614341162
1201	18	0.9138508839696371
1201	19	0.8216987615248215
1201	20	1.0064884997681034
1201	21	0.9554111144568935
1201	22	1.1556197041570044
1201	23	0.9705424399521974
1201	24	1.0168076011632792
1201	25	0.9782716929572288
1202	1	0.551259778914023
1202	2	0.6447345882969913
1202	3	0.6514536161052611
1202	4	0.6171941717304676
1202	5	0.6977504922909148
1202	6	0.7427700152623373
1202	7	0.6216644170548733
1202	8	0.6362989018961045
1202	9	0.7347035547849785
1202	10	0.7459718429977897
1202	11	0.7528094865875374
1202	12	0.7244014311715787
1202	13	0.7304692254427394
1202	14	0.6874519390497795
1202	15	0.8866140837378751
1202	16	0.6531566935830284
1202	17	0.8473813984931766
1202	18	0.8588061667496163
1202	19	0.9755727353188348
1202	20	0.9849623797514653
1202	21	0.9301200329608079
1202	22	0.9121909866855233
1202	23	0.8685642401699454
1202	24	0.795315401909761
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2211	9	-0.25152579966763255
2211	10	-0.3032713981095796
2211	11	-0.1946727022014869
2211	12	-0.2205746693945656
2211	13	-0.02860397401614989
2211	14	-1.1601293232298602
2211	15	-0.5298099627374171
2211	16	-0.3030594604142678
2211	17	-0.11979748996500916
2211	18	-0.40193289056661996
2211	19	-0.283440741551253
2211	20	-0.34857910104503276
2211	21	-0.34640604193954516
2211	22	-0.2111780689841538
2211	23	-0.2888975977287558
2211	24	-0.4287032601107512
2211	25	-0.47554240720792507
2212	1	-0.18968964434924374
2212	2	-0.1551508998731883
2212	3	-0.09454581602652112
2212	4	0.09053667096664242
2212	5	-0.09518312412684793
2212	6	-0.056697183444455845
2212	7	-0.03902175468135738
2212	8	-0.2734228851611036
2212	9	-0.43065027066068495
2212	10	-0.2998269588852409
2212	11	-0.2584283917803276
2212	12	-0.15776228481075805
2212	13	-0.3802666701658026
2212	14	-0.28852288073879606
2212	15	-0.27515250684719206
2212	16	-0.2731909932921681
2212	17	-0.41186248001842785
2212	18	-0.2173779216820506
2212	19	-0.20610747840353838
2212	20	-0.5160503303712787
2212	21	-0.3252400199174943
2212	22	-0.3505898540639052
2212	23	-0.7099775280598095
2212	24	-0.3817016720182522
2212	25	-0.6198462098852886
2213	1	-0.39916385325038206
2213	2	-0.40257190923482966
2213	3	-0.3454916219598161
2213	4	-0.37057770715743743
2213	5	-0.3625053247065324
2213	6	-0.1950290523004199
2213	7	-0.6794483826617466
2213	8	-0.3177830062215392
2213	9	-0.5511613976790812
2213	10	-0.27838917373377825
2213	11	-0.3315980307284505
2213	12	-0.5491515837798104
2213	13	-0.17748311833063468
2213	14	-0.24352026921052783
2213	15	-0.4059313862102627
2213	16	-0.6759500652439456
2213	17	-0.508186817860576
2213	18	-0.7071338485565306
2213	19	-0.8677209599540205
2213	20	-0.31301516553835995
2213	21	-1.065044668063038
2213	22	-0.5703934834919622
2213	23	-0.6025905656675192
2213	24	-0.8910109270495781
2213	25	-0.8658426331307183
2214	1	-1.1262910874387408
2214	2	-1.122599974319737
2214	3	-0.9035672847429232
2214	4	-1.5228537708728496
2214	5	-1.380910420822552
2214	6	-2.839268396675436
2214	7	-1.4994811582549872
2214	8	-1.9328675739864423
2214	9	-1.5599506052593455
2214	10	-1.805778039983359
2214	11	-1.5424400301156425
2214	12	-1.6137013674455147
2214	13	-1.8361896445587504
2214	14	-2.0206389882492104
2214	15	-2.375810646071862
2214	16	-1.7521065237618245
2214	17	-1.905552751093147
2214	18	-1.9324217308754825
2214	19	-3.7913631780219212
2214	20	-2.2553413099996646
2214	21	-2.6962888423440674
2214	22	-2.4667459504152234
2214	23	-2.5357589693451814
2214	24	-1.748127864402555
2214	25	-1.5917353252763746
2215	1	-1.4851410855962435
2215	2	-1.2683091999873533
2215	3	-1.226363048688512
2215	4	-1.1579862241589112
2215	5	-1.888752839882578
2215	6	-1.7462692166972857
2215	7	-1.9360014774375038
2215	8	-2.77060021134454
2215	9	-2.2082649431216623
2215	10	-1.6585319504042957
2215	11	-2.1301111103379355
2215	12	-2.822440394048055
2215	13	-2.17967189155182
2215	14	-2.350349219076257
2215	15	-3.5616366549273835
2215	16	-2.948248203292671
2215	17	-2.5381065086978793
2215	18	-3.307343535775992
2215	19	-3.9467792958244736
2215	20	-3.4011141128894096
2215	21	-2.4493809285338273
2215	22	-3.434518336856403
2215	23	-3.283274107783253
2215	24	-2.387933154720038
2215	25	-2.5999746136021855
2216	1	-0.9268071708659846
2216	2	-0.7339969226391894
2216	3	-1.3702400830248855
2216	4	-0.7981187912182293
2216	5	-0.74899851610855
2216	6	-0.8658600102805494
2216	7	-2.173044237290899
2216	8	-1.483472082448202
2216	9	-1.536876134962533
2216	10	-1.4500936624420362
2216	11	-1.8201459385105352
2216	12	-1.3740912545654425
2216	13	-1.859777202152607
2216	14	-1.803343513111301
2216	15	-1.796631242585704
2216	16	-1.7184656428890932
2216	17	-1.9032551372931081
2216	18	-1.812185109750871
2216	19	-1.8513376046570968
2216	20	-2.4157979306940263
2216	21	-1.9966059531130043
2216	22	-2.4404106627166087
2216	23	-1.4603185882861922
2216	24	-1.7025132627271802
2216	25	-1.455964378733519
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57168.0
3	1.0
4	954.0
5	8159.0
6	9734.0
7	7535.0
8	5975.0
9	5356.0
10	4515.0
11	4133.0
12	3874.0
13	3614.0
14	3388.0
15	3650.0
16	3643.0
17	3826.0
18	4205.0
19	4866.0
20	6260.0
21	8182.0
22	10063.0
23	12764.0
24	15003.0
25	17186.0
26	19618.0
27	21605.0
28	24568.0
29	29553.0
30	36952.0
31	47520.0
32	60058.0
33	77746.0
34	101685.0
35	133906.0
36	183161.0
37	286662.0
38	938792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.82269644459518	19.174400863506943	13.229473458528288	27.77342923336959
2	13.68396557885532	20.689761842541454	39.37613291238011	26.25013966622312
3	16.884407261713484	24.822196982288954	30.245165937171038	28.048229818826524
4	11.60489596558064	17.35741998888834	37.37798995332272	33.65969409220831
5	12.81571376363471	37.97375833999023	36.052505496851126	13.158022399523938
6	31.945448824078742	37.292024103879015	17.876997393413436	12.88552967862881
7	27.37918631116892	31.601106989255257	22.708798273747064	18.310908425828764
8	25.103169850932733	36.748703562016495	20.54877305035317	17.599353536697603
9	26.28253275795009	14.857322878874808	19.781247199533635	39.07889716364147
10	14.507553610179635	29.0438102457321	34.8483384830844	21.600297661003868
11	34.09723783742104	22.323498489936526	24.594018938933964	18.985244733708466
12	22.930428675065215	24.751273704903134	31.914036867597957	20.404260752433697
13	28.859416470118887	21.682344314767033	25.680513973207937	23.777725241906143
14	21.04440023937231	21.742846535750697	27.501588427383012	29.71116479749398
15	23.24438513538473	30.270076834989474	23.259391969266005	23.226146060359795
16	22.42212874197435	27.708216630545568	27.384766147240164	22.484888480239917
17	22.028262868602187	28.11410450804624	27.612666516717137	22.244966106634433
18	22.017782288168736	27.358655649041513	28.998602404676564	21.624959658113184
19	23.454354384581357	26.86945295812991	27.46632412870291	22.209868528585822
20	23.57178015069228	27.201305051672918	27.556472666592175	21.670442131042627
21	22.960306610209166	26.69320556984538	27.178736373302826	23.167751446642633
22	23.25103260663805	27.454177936153375	26.867579799046855	22.427209658161726
23	22.40045616562321	27.34809869429516	27.319472916320386	22.931972223761242
24	23.01729551036992	27.043511182521655	27.663213105065836	22.27598020204259
25	22.840745127100547	26.903345407365855	27.400250715092078	22.85565875044152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206.0
1	206.0
2	347.5
3	489.0
4	489.0
5	489.0
6	1159.0
7	1829.0
8	1829.0
9	1829.0
10	2684.0
11	3539.0
12	3539.0
13	3539.0
14	6163.0
15	8787.0
16	8787.0
17	8787.0
18	15989.0
19	23191.0
20	23191.0
21	23191.0
22	37438.5
23	51686.0
24	51686.0
25	51686.0
26	74635.0
27	97584.0
28	97584.0
29	97584.0
30	126209.5
31	154835.0
32	154835.0
33	154835.0
34	176585.0
35	198335.0
36	198335.0
37	198335.0
38	216763.0
39	235191.0
40	235191.0
41	235191.0
42	250039.5
43	264888.0
44	264888.0
45	264888.0
46	277954.5
47	291021.0
48	291021.0
49	291021.0
50	281135.5
51	271250.0
52	271250.0
53	271250.0
54	243400.0
55	215550.0
56	215550.0
57	215550.0
58	185956.0
59	156362.0
60	156362.0
61	156362.0
62	127365.0
63	98368.0
64	98368.0
65	98368.0
66	75858.0
67	53348.0
68	53348.0
69	53348.0
70	38951.5
71	24555.0
72	24555.0
73	24555.0
74	17059.0
75	9563.0
76	9563.0
77	9563.0
78	6605.5
79	3648.0
80	3648.0
81	3648.0
82	2411.0
83	1174.0
84	1174.0
85	1174.0
86	782.5
87	391.0
88	391.0
89	391.0
90	232.5
91	74.0
92	74.0
93	74.0
94	43.5
95	13.0
96	13.0
97	13.0
98	8.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07765896540897926
2	9.23412192734593E-5
3	0.0
4	0.0271021478567603
5	0.06736291945998855
6	0.08066005503536669
7	0.033612203815539185
8	0.0687018671394537
9	0.04903318743420688
10	0.10827007959813102
11	0.0035551369420281824
12	0.016021201543945186
13	0.007571979980423662
14	0.009972851681533603
15	0.009234121927345929
16	0.0221157220159935
17	0.03264262101316786
18	7.849003638244039E-4
19	0.00397067242875875
20	0.006556226568415609
21	1.3851182891018893E-4
22	9.23412192734593E-5
23	0.0
24	0.0
25	0.003462795722754723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2165880.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.17325719966274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88724829957827	55.14347182500132
2	12.546630345244683	17.106893150309183
3	3.4195718960039567	6.993700631370483
4	1.2612653681603092	3.4393827336248033
5	0.5999732291120166	2.0451064630582843
6	0.3377036816501746	1.3813415967846228
7	0.21843308103808592	1.0423906230166944
8	0.1338134414381402	0.729799852794747
9	0.10019926747107974	0.6147819389271347
>10	0.4504602088139362	5.23622139363445
>50	0.02576060163729499	1.181085455064132
>100	0.015209601364906357	2.0617989194476496
>500	0.0022385870922586137	1.0352627769417384
>1k	0.0014923913948387494	1.9887626400247942
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	4241	0.1958095554693704	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4018	0.1855135095203797	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3672	0.16953847858607124	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2733	0.1261842761371821	No Hit
GTCCTACAGTGGACATTTCTAAATT	2725	0.12581491126008826	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2641	0.121936580050603	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2628	0.1213363621253255	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2461	0.11362587031599165	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2338	0.10794688533067391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.234121927345928E-5	0.0	0.0	0.0	0.0
7	9.234121927345928E-5	0.0	0.0	0.0	0.0
8	9.234121927345928E-5	0.0	0.0	0.0	0.0
9	9.234121927345928E-5	0.0	0.0	0.0	0.0
10	9.234121927345928E-5	0.0	0.0	0.0	0.0
11	9.234121927345928E-5	0.0	0.0	0.0	0.0
12	9.234121927345928E-5	0.0	0.0	0.0	0.0
13	9.234121927345928E-5	0.0	0.0	0.0	9.234121927345928E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCCGT	25	0.0060061747	19.014568	1
GGCGAGG	730	0.0	12.220777	19
TAGGACC	480	0.0	11.892082	4
TGGCGAG	1520	0.0	11.0515585	18
TCCAACG	140	8.485586E-9	10.846405	18
GTATTAA	255	0.0	10.812206	1
CCAACGA	80	3.8066102E-4	10.676929	19
AGGACGT	1435	0.0	10.607572	5
CGGTTTC	100	2.4191248E-5	10.442316	13
GGACGTG	1465	0.0	10.257624	6
TAGGACG	1540	0.0	10.193213	4
CTAGGAC	285	0.0	10.007437	3
GACGTGA	865	0.0	9.997953	7
ATGGCGA	1730	0.0	9.986871	17
ACTATAC	210	5.456968E-12	9.959783	3
GTCCTAA	890	0.0	9.934579	1
ATTATAC	250	0.0	9.887347	3
TTAGGAC	1060	0.0	9.865822	3
AGGACCT	1120	0.0	9.85344	5
GTAGGAC	1420	0.0	9.841821	3
>>END_MODULE