FastQCFastQC Report
Thu 26 May 2016
SRR1513012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818950
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39940.21957722862090767No Hit
GTCCTAAAGTGTGTATTTCTCATTT37010.2034690343329943No Hit
CTGTAGGACGTGGAATATGGCAAGA36180.1989059622309574No Hit
GTACATGGGAAGCAGTGGTATCAAC33810.18587646719261114No Hit
CTTTAGGACGTGAAATATGGCGAGG33230.18268781439841666No Hit
CCCATGTACTCTGCGTTGATACCAC28510.15673877786635146No Hit
GTATCAACGCAGAGTACTTTTTTTT27430.15080128645647214No Hit
GTCCTACAGTGTGCATTTCTCATTT21200.11655075730503864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGT456.7855633E-414.7713196
GAACCGT801.2957207E-714.2437726
CTCGACA500.001500618313.2989414
TAGGACC6100.012.92519954
TCCAACG1051.9934305E-812.66705118
GACGGCG550.003066828512.09194113
GCGCCAC752.0725396E-411.40097313
GCCCTAG1451.1623342E-911.1693041
TAACACT2100.010.8562784
CCATGCG700.001496376110.8529959
AGGACCT12200.010.7447515
GGACCTG12350.010.5338556
GTCCTAA12300.010.4561991
GGCGAGG9050.010.38772319
TGTAGGA21100.010.2195652
TGGCGAG19800.010.17202718
GTATTAA2350.010.134851
GTAGGAC20500.010.1016353
GGCCTAA856.4257334E-410.0871571
GTCCTAC21100.010.0685641
Produced by FastQC (version 0.11.5)