##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513012_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1818950
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.687041974765663	31.0	30.0	34.0	18.0	34.0
2	28.70468622007202	31.0	30.0	34.0	16.0	34.0
3	28.965532862365652	31.0	30.0	34.0	22.0	34.0
4	32.38211770526952	35.0	33.0	37.0	23.0	37.0
5	32.50212705132082	35.0	33.0	37.0	25.0	37.0
6	32.50010005772561	36.0	33.0	37.0	24.0	37.0
7	32.24153934962478	35.0	33.0	37.0	22.0	37.0
8	32.29627367437258	35.0	33.0	37.0	22.0	37.0
9	33.56984743945683	38.0	34.0	39.0	19.0	39.0
10	33.43488276203304	38.0	33.0	39.0	18.0	39.0
11	33.52623931388988	38.0	34.0	39.0	19.0	39.0
12	33.441378817449625	38.0	33.0	39.0	18.0	39.0
13	33.45699936776712	38.0	34.0	39.0	18.0	39.0
14	34.38390115176338	39.0	34.0	41.0	17.0	41.0
15	34.333385744522936	39.0	34.0	41.0	17.0	41.0
16	34.2326743450892	39.0	33.0	41.0	17.0	41.0
17	34.15486297039501	39.0	33.0	41.0	17.0	41.0
18	33.8763335990544	39.0	33.0	41.0	11.0	41.0
19	34.09725665906155	39.0	34.0	41.0	17.0	41.0
20	34.183160614640315	39.0	34.0	41.0	17.0	41.0
21	34.109317463371724	39.0	34.0	41.0	11.0	41.0
22	34.004611451661674	39.0	34.0	41.0	10.0	41.0
23	33.74190824376701	39.0	33.0	41.0	2.0	41.0
24	33.749672613320875	39.0	34.0	41.0	2.0	41.0
25	33.639450782044584	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8250571620814497
1101	2	1.114800753327021
1101	3	1.174476128973037
1101	4	1.0644124596561042
1101	5	0.8910023185768949
1101	6	1.0938367334901429
1101	7	1.216978585335582
1101	8	0.9989143844824682
1101	9	1.0298503996929682
1101	10	1.0154953634922421
1101	11	1.1930935068160622
1101	12	1.426423169848654
1101	13	1.1672598818073823
1101	14	1.2197261894753098
1101	15	1.0581834059947326
1101	16	1.4753148218515477
1101	17	1.5802346271458916
1101	18	1.7545830079604343
1101	19	1.292345787692689
1101	20	1.4100857968146556
1101	21	1.3196666113587838
1101	22	1.4555896884463166
1101	23	1.7971338671550043
1101	24	1.3961169025024063
1101	25	1.7342631030312816
1102	1	1.0184800588494411
1102	2	0.9767497591334191
1102	3	1.1009179987507345
1102	4	1.3085859718907074
1102	5	1.1246085581252458
1102	6	1.1073583164166934
1102	7	1.346556162328831
1102	8	1.3426701126224785
1102	9	1.4415954484650015
1102	10	1.5458640230581295
1102	11	1.5934347982102466
1102	12	1.6079868780455726
1102	13	1.4393215046088343
1102	14	1.4786790079316887
1102	15	1.83920595477381
1102	16	1.7839826080611445
1102	17	1.574140238678595
1102	18	1.9630407741197828
1102	19	1.9012382579909612
1102	20	1.763225452040551
1102	21	1.6792943985835933
1102	22	1.920057242851314
1102	23	2.0785945745820555
1102	24	1.8566040164786415
1102	25	1.8142822226813777
1103	1	0.8061479304793515
1103	2	0.9938521873943209
1103	3	1.0273259631837952
1103	4	1.2105713107444345
1103	5	0.8085692066344592
1103	6	1.1209762938115446
1103	7	0.9271339745862548
1103	8	1.0249998192079985
1103	9	1.388186205183743
1103	10	1.3510289461311231
1103	11	1.2683429437070473
1103	12	1.4810521868709898
1103	13	1.4522226383323869
1103	14	1.6855076194692344
1103	15	1.7213130487326609
1103	16	1.6951572739141625
1103	17	1.6863855066210078
1103	18	1.9327415583036611
1103	19	1.99945484853788
1103	20	1.83577181028771
1103	21	1.5562661462546146
1103	22	1.6426419346187942
1103	23	2.086036431228834
1103	24	1.8104335602436166
1103	25	1.7247706022201683
1104	1	0.6393295596987763
1104	2	0.6644842850977426
1104	3	0.626756701479291
1104	4	0.8511498957616723
1104	5	0.6752954892956708
1104	6	0.6766941407112768
1104	7	0.8419248592795299
1104	8	0.7893281490074884
1104	9	0.9922763325052273
1104	10	1.0216837172604656
1104	11	0.9615826010254196
1104	12	1.0386282343917728
1104	13	0.8875425530032217
1104	14	1.1835644501845621
1104	15	1.3331979597831136
1104	16	1.1126011194904493
1104	17	1.1559801590481626
1104	18	1.4437214482219076
1104	19	1.1573164225112365
1104	20	1.2445163158080632
1104	21	1.2241209760174314
1104	22	1.0787600672490427
1104	23	1.4373874669906073
1104	24	1.3144946542432194
1104	25	1.1233006159976782
1105	1	0.8142731205631186
1105	2	1.0082969196836977
1105	3	0.9434026635538615
1105	4	0.9825758131449973
1105	5	0.8127598106622074
1105	6	0.6046019049389528
1105	7	0.9501962132087556
1105	8	0.9174248311271427
1105	9	0.9715980886320068
1105	10	1.1851795574923543
1105	11	1.0059297061355537
1105	12	1.1387199871007994
1105	13	1.0833848032933773
1105	14	1.2620573874135133
1105	15	1.4292171641426776
1105	16	1.3315395236202576
1105	17	1.272655280826001
1105	18	1.3844341109189031
1105	19	1.4492278297849026
1105	20	1.4901074329460684
1105	21	1.418372635532684
1105	22	1.201169592391281
1105	23	1.3802146028345703
1105	24	1.474826226906302
1105	25	1.6488131575454616
1106	1	1.0380959443189823
1106	2	0.9413356474997947
1106	3	1.0292048203302393
1106	4	1.0676991486868133
1106	5	1.0766460193090452
1106	6	1.254236167562837
1106	7	1.0043147316727712
1106	8	1.1919485442179862
1106	9	1.2361058161461003
1106	10	1.3724128130215547
1106	11	1.1951971957043455
1106	12	1.3125231953867669
1106	13	1.3491799388575103
1106	14	1.5151141175375287
1106	15	1.6157583319359432
1106	16	1.5089260250777343
1106	17	1.6765205819125981
1106	18	1.7703743619411725
1106	19	1.3693995005424497
1106	20	1.404869715636032
1106	21	1.3758546134364877
1106	22	1.4760232957685844
1106	23	1.7445180819689767
1106	24	1.6626086487027862
1106	25	1.847391389936881
1107	1	0.6490793078694246
1107	2	0.6478565749548046
1107	3	0.8259226216853364
1107	4	0.9664283316160578
1107	5	0.7971588067314102
1107	6	0.8108633760615263
1107	7	1.0356668398715172
1107	8	0.9733338807540335
1107	9	1.1738615571479443
1107	10	0.9190394225829124
1107	11	0.9362843077752316
1107	12	1.0893202086859333
1107	13	1.057338292593876
1107	14	1.384567728958686
1107	15	1.5210907162348732
1107	16	1.3760520535154512
1107	17	1.438807979840938
1107	18	1.7524225665796394
1107	19	1.5534183082777204
1107	20	1.4507110334217046
1107	21	1.4676064080700328
1107	22	1.5300107127901157
1107	23	1.5027739588071682
1107	24	1.5920243803709226
1107	25	1.6132586241711238
1108	1	0.6274723228881562
1108	2	0.6172876696826357
1108	3	0.5069513344343264
1108	4	0.6345704583866763
1108	5	0.5206474259522196
1108	6	0.6574990154554357
1108	7	0.7192651137345578
1108	8	0.564717647474076
1108	9	0.7771660456653038
1108	10	0.7576836462577035
1108	11	0.6770869981411849
1108	12	0.6490027043145759
1108	13	0.7224777551173034
1108	14	0.9562331497172707
1108	15	0.8885028211580845
1108	16	0.8059726520109507
1108	17	0.9980739451604208
1108	18	1.0838955083509134
1108	19	1.1125873778197146
1108	20	0.9718315507766562
1108	21	0.9236366068489019
1108	22	0.8206709763068574
1108	23	1.0227687394724754
1108	24	1.0430804117629435
1108	25	1.0852840287634749
1109	1	0.14441787325955957
1109	2	0.09752098577870427
1109	3	0.2838052630828187
1109	4	0.38660631579323734
1109	5	0.28315688754997126
1109	6	0.29095273272100286
1109	7	0.3468579732399064
1109	8	0.42385542338168136
1109	9	0.5931676928983123
1109	10	0.8390801073563026
1109	11	0.12394711738594566
1109	12	0.4723009246697103
1109	13	0.4448177241620783
1109	14	0.37988661643995414
1109	15	0.5880245936670292
1109	16	0.6436594617297118
1109	17	0.48510647599159284
1109	18	-1.0487461562705604
1109	19	0.25694390946013357
1109	20	0.6536782390995555
1109	21	0.6383933761435472
1109	22	0.8287745498998476
1109	23	0.4478674645509457
1109	24	0.7360478315327512
1109	25	0.95163024543794
1110	1	0.33398134157150494
1110	2	0.3858182517023039
1110	3	0.4248124360112122
1110	4	0.22681356988859847
1110	5	0.5046863573193789
1110	6	0.41541843311322424
1110	7	0.4359042602946417
1110	8	0.39876728954956775
1110	9	0.6457353108039356
1110	10	0.8873956627826658
1110	11	0.7547718879899463
1110	12	0.26291080932890054
1110	13	0.4453845012174611
1110	14	0.6760944065605017
1110	15	0.795134918448241
1110	16	0.8940666152866754
1110	17	0.35388423193379737
1110	18	0.9329449246451134
1110	19	0.7261390153346454
1110	20	0.6987681035657687
1110	21	0.6706196461141545
1110	22	0.6604190933687804
1110	23	0.7471710919577674
1110	24	0.8961943361064115
1110	25	0.8620501778534262
1111	1	0.2136280043993537
1111	2	0.5611867881043366
1111	3	0.44654913164036714
1111	4	0.3719408899707588
1111	5	0.42947514576914614
1111	6	0.5231970636601133
1111	7	0.41465929932462586
1111	8	0.2653633284803405
1111	9	0.590581244217617
1111	10	0.5407913476365778
1111	11	0.3649192304420765
1111	12	0.4880458473656404
1111	13	0.5108801152071507
1111	14	0.5515690008706855
1111	15	0.5813366438693279
1111	16	0.512848390353426
1111	17	0.41913025685572336
1111	18	0.5672596066813185
1111	19	0.29276935240898894
1111	20	0.4549719213015493
1111	21	0.5736262639461884
1111	22	0.2259716383224344
1111	23	0.6184339579776363
1111	24	0.7396223221587377
1111	25	0.07330627263586109
1112	1	-0.14898541090396478
1112	2	0.0814153318272659
1112	3	0.1138517368355636
1112	4	0.2543031052022755
1112	5	0.20547883840735182
1112	6	0.31065762221437865
1112	7	-0.10035640839660331
1112	8	0.3190087995968227
1112	9	0.47513964367550443
1112	10	0.5369960174277679
1112	11	0.5693201229246228
1112	12	0.6205396626952648
1112	13	0.17420005148824202
1112	14	0.38515263359374785
1112	15	0.3631864200719903
1112	16	0.6257045268119867
1112	17	0.6296249996187839
1112	18	0.7295891884126391
1112	19	0.5691967534370477
1112	20	0.31595567981934636
1112	21	0.4470783749257379
1112	22	0.2582897019287955
1112	23	0.5775817734397535
1112	24	0.3186694021086751
1112	25	0.6542762454943016
1113	1	-0.5069930439155925
1113	2	-0.4078850927942739
1113	3	-0.45884839460926585
1113	4	-0.215939989362532
1113	5	-0.423111750374332
1113	6	-0.21960278130268307
1113	7	-0.3537565275674588
1113	8	-0.06797927150154237
1113	9	-0.3202000812862593
1113	10	-0.5678951963619028
1113	11	-0.5233991211916518
1113	12	-0.4697791639612916
1113	13	-0.44597177446635783
1113	14	-0.4667863765840039
1113	15	-0.8072784258510808
1113	16	-0.8440146934315322
1113	17	-0.8574463367514511
1113	18	-0.9523637571837682
1113	19	-0.8226723312743331
1113	20	-1.0223152142056406
1113	21	-0.9146112083789646
1113	22	-0.7052962702481125
1113	23	-1.1368007409013856
1113	24	-0.43673373756839595
1113	25	-0.5003674753565193
1114	1	-2.197468010378543
1114	2	-1.8797134598309029
1114	3	-1.6748974552521823
1114	4	-1.965883273704275
1114	5	-2.00074902848311
1114	6	-2.500651183353394
1114	7	-2.5589957244174713
1114	8	-2.100911183298784
1114	9	-2.3020193559140907
1114	10	-2.5150401672112075
1114	11	-3.290332677551156
1114	12	-2.5951029931058507
1114	13	-2.4249560334734035
1114	14	-2.9325651586615997
1114	15	-2.936277838872794
1114	16	-3.66088130357436
1114	17	-5.228320064805306
1114	18	-4.489107191403196
1114	19	-3.46446326725534
1114	20	-2.6995982592290524
1114	21	-3.637405531953622
1114	22	-3.88880537736334
1114	23	-3.8173198394357293
1114	24	-3.4505709514804224
1114	25	-3.2031431537384556
1115	1	-1.6668874780557417
1115	2	-1.1961428160656276
1115	3	-1.3656485147404887
1115	4	-1.2477480852386833
1115	5	-1.0965974039343749
1115	6	-1.4069966213873428
1115	7	-2.6005088867378063
1115	8	-1.8801107370804289
1115	9	-1.7353158781016838
1115	10	-1.4910253792209822
1115	11	-2.2779935628124974
1115	12	-1.9890375626957315
1115	13	-1.7941658457926195
1115	14	-1.749345155073911
1115	15	-3.0764181510014126
1115	16	-1.9187097449493393
1115	17	-1.99946918918587
1115	18	-2.102541671300944
1115	19	-2.2606479882006916
1115	20	-2.0188161998977066
1115	21	-2.902567145963534
1115	22	-2.6800054580862565
1115	23	-2.3427904428395436
1115	24	-2.1906983703651015
1115	25	-2.682631123781281
1116	1	-2.8867212971448204
1116	2	-3.7838635602084594
1116	3	-3.222056182438603
1116	4	-3.1631994259994585
1116	5	-3.7856806367449884
1116	6	-3.2565220729903395
1116	7	-3.0178113127693216
1116	8	-4.109549314577826
1116	9	-4.213523364484288
1116	10	-5.403650689218061
1116	11	-3.682478405734823
1116	12	-3.793666352901699
1116	13	-4.712036287618062
1116	14	-4.430159145588565
1116	15	-3.8878205178431493
1116	16	-3.8627164056720567
1116	17	-4.316502076972547
1116	18	-3.8623815030221706
1116	19	-4.324941944100566
1116	20	-4.630609481483692
1116	21	-5.252998238576797
1116	22	-4.12816383194086
1116	23	-4.127266145062531
1116	24	-4.618441423058986
1116	25	-4.63911446448212
1201	1	0.5647878539029492
1201	2	0.6034359368913265
1201	3	0.7575952350750335
1201	4	0.7346625315361379
1201	5	0.7929137976949505
1201	6	0.8051509689868013
1201	7	0.7890405286185214
1201	8	0.25671575256971124
1201	9	0.5810900918839366
1201	10	0.8801108176645727
1201	11	0.4969094006752073
1201	12	-0.1058313624789946
1201	13	0.33454114926963996
1201	14	0.9301220559670611
1201	15	0.6106812637224195
1201	16	1.183407348235228
1201	17	1.205846313885985
1201	18	1.4733868959109415
1201	19	1.1332422430002609
1201	20	0.9500048249259265
1201	21	0.9655541196329409
1201	22	1.1889536105318896
1201	23	1.3618393214898106
1201	24	1.2184333399653582
1201	25	1.190468204500462
1202	1	1.1829186831014553
1202	2	1.2972145560632669
1202	3	1.0126634198962208
1202	4	1.4136081613743485
1202	5	1.3513891732468366
1202	6	1.2910540476693058
1202	7	1.3042938353431452
1202	8	1.4081177217965575
1202	9	1.6321640085130156
1202	10	1.8203970435561203
1202	11	1.4074273592767952
1202	12	1.576523736232943
1202	13	1.611420047741916
1202	14	1.8849075232369756
1202	15	1.6345384332663784
1202	16	1.8527886501484119
1202	17	1.9526541798582784
1202	18	2.350544639091318
1202	19	2.0321362045543196
1202	20	1.9005265196821028
1202	21	1.9036237123465227
1202	22	2.063193508106579
1202	23	1.9856251851566213
1202	24	2.183081799712852
1202	25	2.0281060891079576
1203	1	0.9485232171916707
1203	2	1.1492703619847262
1203	3	1.1821363138730874
1203	4	1.4703518089813912
1203	5	1.1535705503820495
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2211	21	0.32672993918342996
2211	22	0.3546817820956747
2211	23	0.8484570506802882
2211	24	0.725166426426604
2211	25	0.6548939410203971
2212	1	0.04161125980731839
2212	2	-0.028974087734056297
2212	3	0.05738653181717979
2212	4	-0.07252027213969114
2212	5	0.1296251930302148
2212	6	-0.05515225177499161
2212	7	-0.05362743857968866
2212	8	0.048902832200937496
2212	9	0.10846639542067749
2212	10	0.17919827039723657
2212	11	0.1141053813916244
2212	12	0.1013995242293646
2212	13	-0.05489558090097546
2212	14	-0.08204982026721552
2212	15	-0.1747885017812152
2212	16	0.08577188860150642
2212	17	-0.003435886576752978
2212	18	-2.896163035456258
2212	19	-1.0524598093438442
2212	20	-0.31239435812045
2212	21	-0.29918647813074273
2212	22	0.1433178898759948
2212	23	0.023130419603241137
2212	24	-0.5593228767700538
2212	25	-0.17111242215883493
2213	1	-0.33662287961449877
2213	2	-0.16612861598541073
2213	3	-0.5546556472998603
2213	4	-0.5491060356576405
2213	5	-0.5141366709500801
2213	6	-0.4311726644428546
2213	7	-0.1539308621749207
2213	8	-0.7273865709286511
2213	9	-0.5113564946669982
2213	10	-0.4939194585463156
2213	11	-0.44400862465330704
2213	12	-0.733784075004273
2213	13	-0.5730353676509878
2213	14	-0.5201717135598187
2213	15	-0.7340538105463565
2213	16	-1.3461302624392886
2213	17	-1.0692843984473583
2213	18	-0.6472715174848105
2213	19	-0.49421925417578194
2213	20	-1.457488519497069
2213	21	-0.9401972230341968
2213	22	-0.8379022202511734
2213	23	-1.2593231030240375
2213	24	-0.9363233093914758
2213	25	-1.17069628155393
2214	1	-2.073390270615505
2214	2	-4.011562029535813
2214	3	-2.1815269766084526
2214	4	-1.8963942291996716
2214	5	-1.9229520837373322
2214	6	-2.078810096517909
2214	7	-2.2694000592834023
2214	8	-1.9275296787085168
2214	9	-2.3097161779517563
2214	10	-2.3682258586515985
2214	11	-1.8442231540608276
2214	12	-2.547145871178852
2214	13	-2.230550675355861
2214	14	-3.6523850499616337
2214	15	-3.907741157080789
2214	16	-3.0506293620638623
2214	17	-3.522556203371085
2214	18	-2.771887973424608
2214	19	-5.15147043412059
2214	20	-3.284969181099413
2214	21	-3.2167826343731747
2214	22	-3.2434641133739994
2214	23	-3.375970624726623
2214	24	-3.5770137978162104
2214	25	-3.105046976294588
2215	1	-1.803422398929463
2215	2	-3.611784435032053
2215	3	-2.30011560933621
2215	4	-1.89192907391719
2215	5	-1.876393636152752
2215	6	-2.364082077052988
2215	7	-2.8542519227416356
2215	8	-2.75170142365344
2215	9	-2.568921144590206
2215	10	-4.288266377069398
2215	11	-3.250195934809888
2215	12	-3.5074108113984543
2215	13	-2.7107943045077327
2215	14	-4.338642660094678
2215	15	-4.256785502545611
2215	16	-3.730335290308151
2215	17	-3.9799325382083417
2215	18	-2.7153287085369833
2215	19	-3.620369285074087
2215	20	-3.5794335627848923
2215	21	-3.292144822729039
2215	22	-2.7497355666684804
2215	23	-3.336560009489734
2215	24	-3.427506925841854
2215	25	-3.259624623653526
2216	1	-3.5495431076710737
2216	2	-3.48819886430163
2216	3	-4.029311313140191
2216	4	-4.887692199757552
2216	5	-4.063233601374961
2216	6	-3.978364869425768
2216	7	-4.076887429968149
2216	8	-4.3556281934867265
2216	9	-4.6669716061269355
2216	10	-4.3212926283986945
2216	11	-5.356897266810588
2216	12	-5.488000473509846
2216	13	-5.055327802030483
2216	14	-5.40350169687353
2216	15	-5.0980714093161055
2216	16	-4.311017590112684
2216	17	-4.784889427943483
2216	18	-4.871627755380871
2216	19	-5.250073652751546
2216	20	-4.7668969698418024
2216	21	-5.307501031527931
2216	22	-6.222623578381032
2216	23	-5.986958850604843
2216	24	-5.649247685091389
2216	25	-6.049951364809893
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97533.0
3	2.0
4	836.0
5	4766.0
6	6066.0
7	5358.0
8	4923.0
9	4559.0
10	4145.0
11	3798.0
12	3639.0
13	3605.0
14	3570.0
15	3651.0
16	3812.0
17	4091.0
18	4572.0
19	5602.0
20	7161.0
21	9012.0
22	11403.0
23	13789.0
24	15868.0
25	18114.0
26	20091.0
27	22245.0
28	25588.0
29	30027.0
30	36449.0
31	46128.0
32	56737.0
33	72184.0
34	92711.0
35	121035.0
36	164980.0
37	255693.0
38	635207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24706341889707	18.809897785847365	12.389335125267094	25.553703669988476
2	14.515413159800882	20.276050354770245	41.716041906943005	23.49249457848587
3	18.53921152378166	25.10124886431882	29.840036693776355	26.519502918123163
4	12.10735968066858	17.089928554104688	38.691766315660956	32.110945449565776
5	12.691032348795217	38.32939265091604	36.29173259678596	12.687842403502781
6	31.352505777742095	37.51235141868794	18.486905159250135	12.648237644319831
7	27.57059450376273	31.63102254119319	22.9031697556287	17.895213199415384
8	24.490064134817843	37.71125089564936	20.938206105757835	16.860478863774965
9	26.608782199151527	15.083101236414425	20.312939048095615	37.99517751633843
10	14.86616154534352	28.97678564456857	35.10037815364024	21.056674656447665
11	33.767514904371176	22.36705938763682	25.393793227075424	18.471632480916576
12	23.408285832745875	24.774026242539485	32.29051993485005	19.527167989864587
13	28.940603429172214	22.28934444754211	25.79720473140204	22.972847391883636
14	20.51896973739709	22.81086794295578	27.749582198895872	28.920580120751254
15	22.751209565216435	31.771392998984833	23.24410945314124	22.23328798265749
16	21.645219223624924	28.151707345506377	28.605474459378165	21.597598971490537
17	21.51664089496363	29.02945892621081	28.164530511423504	21.28936966740206
18	21.61279194709892	27.452091543650596	30.246354907979338	20.688761601271143
19	23.338319154903246	27.19641020846966	27.598062128469223	21.86720850815787
20	23.510550490750553	27.622253223875624	28.244565286127727	20.6226309992461
21	22.50522005623418	27.048222486303025	27.35512934796177	23.091428109501024
22	22.99601887186706	28.681993643392094	27.05220556698083	21.26978191776001
23	21.900784863403633	28.379193558764126	27.89644180226589	21.823579775566348
24	22.940109711249143	27.302605532191937	28.48742323731766	21.26986151924126
25	22.42043449307766	27.66999554036138	28.06069507674593	21.848874889815033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	294.0
1	294.0
2	431.5
3	569.0
4	569.0
5	569.0
6	1092.0
7	1615.0
8	1615.0
9	1615.0
10	2447.5
11	3280.0
12	3280.0
13	3280.0
14	5865.0
15	8450.0
16	8450.0
17	8450.0
18	15024.0
19	21598.0
20	21598.0
21	21598.0
22	34912.0
23	48226.0
24	48226.0
25	48226.0
26	69712.0
27	91198.0
28	91198.0
29	91198.0
30	117553.0
31	143908.0
32	143908.0
33	143908.0
34	163195.5
35	182483.0
36	182483.0
37	182483.0
38	195444.0
39	208405.0
40	208405.0
41	208405.0
42	217540.5
43	226676.0
44	226676.0
45	226676.0
46	234845.5
47	243015.0
48	243015.0
49	243015.0
50	230443.5
51	217872.0
52	217872.0
53	217872.0
54	190735.0
55	163598.0
56	163598.0
57	163598.0
58	139644.5
59	115691.0
60	115691.0
61	115691.0
62	94106.5
63	72522.0
64	72522.0
65	72522.0
66	55943.5
67	39365.0
68	39365.0
69	39365.0
70	28981.5
71	18598.0
72	18598.0
73	18598.0
74	13082.5
75	7567.0
76	7567.0
77	7567.0
78	5187.5
79	2808.0
80	2808.0
81	2808.0
82	1843.5
83	879.0
84	879.0
85	879.0
86	549.5
87	220.0
88	220.0
89	220.0
90	151.0
91	82.0
92	82.0
93	82.0
94	50.5
95	19.0
96	19.0
97	19.0
98	15.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3509167376783309
2	0.04018802056131284
3	0.03617471618241293
4	0.035789878776217046
5	0.04051788119519503
6	0.018307265180461257
7	0.01781247422963798
8	0.024024849501085792
9	0.021166057340773523
10	0.020726243162263944
11	0.0195717309436763
12	0.020671266389950246
13	0.020726243162263944
14	0.02754336292916243
15	0.02902773578163226
16	0.021770801836224197
17	0.02298029082712554
18	0.07526320129745183
19	0.025289315264300834
20	0.022485499876302262
21	0.026553781027515877
22	0.02034140575606806
23	0.02292531405481184
24	0.019681684488303694
25	0.02292531405481184
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1818950.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.3628377066858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.68970972142748	61.28387719997986
2	10.532695068601393	15.243514077264248
3	2.5679666843108753	5.574760692388916
4	0.8745057472912815	2.531268698592119
5	0.4176455684238611	1.5111009243386198
6	0.23644765172325596	1.0266013828666158
7	0.13829038380240494	0.7004959219642102
8	0.0947756729247754	0.5486589310717965
9	0.07280822438030657	0.47417487520896784
>10	0.33571938143225094	4.18664383006545
>50	0.019391493287924017	0.9612351284536055
>100	0.015624877757285625	2.2458397839421234
>500	0.0030479480253946195	1.487554566099648
>1k	0.0013715766114275768	2.224273987763868
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3994	0.21957722862090767	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3701	0.2034690343329943	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3618	0.1989059622309574	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3381	0.18587646719261114	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3323	0.18268781439841666	No Hit
CCCATGTACTCTGCGTTGATACCAC	2851	0.15673877786635146	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2743	0.15080128645647214	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2120	0.11655075730503864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTGT	45	6.7855633E-4	14.771319	6
GAACCGT	80	1.2957207E-7	14.243772	6
CTCGACA	50	0.0015006183	13.298941	4
TAGGACC	610	0.0	12.9251995	4
TCCAACG	105	1.9934305E-8	12.667051	18
GACGGCG	55	0.0030668285	12.091941	13
GCGCCAC	75	2.0725396E-4	11.400973	13
GCCCTAG	145	1.1623342E-9	11.169304	1
TAACACT	210	0.0	10.856278	4
CCATGCG	70	0.0014963761	10.852995	9
AGGACCT	1220	0.0	10.744751	5
GGACCTG	1235	0.0	10.533855	6
GTCCTAA	1230	0.0	10.456199	1
GGCGAGG	905	0.0	10.387723	19
TGTAGGA	2110	0.0	10.219565	2
TGGCGAG	1980	0.0	10.172027	18
GTATTAA	235	0.0	10.13485	1
GTAGGAC	2050	0.0	10.101635	3
GGCCTAA	85	6.4257334E-4	10.087157	1
GTCCTAC	2110	0.0	10.068564	1
>>END_MODULE