FastQCFastQC Report
Thu 26 May 2016
SRR1513012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818950
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT37160.20429368591769978No Hit
CTGTAGGACGTGGAATATGGCAAGA36380.2000054976772314No Hit
CTTTAGGACGTGAAATATGGCGAGG36110.19852112482476153No Hit
GTCCTAAAGTGTGTATTTCTCATTT35620.1958272629813904No Hit
GTATCAACGCAGAGTACTTTTTTTT34800.19131916765166718No Hit
GTACATGGGAAGCAGTGGTATCAAC32800.18032381318892768No Hit
CCCATGTACTCTGCGTTGATACCAC32730.1799389757827318No Hit
TATCAACGCAGAGTACTTTTTTTTT25130.13815662882432173No Hit
GGTATCAACGCAGAGTACTTTTTTT21450.11792517661288107No Hit
GTCCTACAGTGTGCATTTCTCATTT20300.11160284779680583No Hit
CTGTAGGACCTGGAATATGGCGAGA18630.10242172682041836No Hit
CTGAAGGACCTGGAATATGGCGAGA18610.10231177327579097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT550.003085136612.082334512
TAGGACC7750.011.907434
GGACCGT657.9503807E-411.7052596
TGTACCG752.0466674E-411.4164025
AAATGTC4850.011.3658947
TAAGACT2200.011.2434274
GGCCCGC600.0058280811.0956116
TCTTGCG600.005847229611.0907222
GGTTCCG600.00585682511.088287
ATTTAGA5800.010.9817561
GGACCTA1303.2137905E-810.97368056
TAGAAAT6900.010.7545824
GGCGAGG8300.010.74815619
AGGACCG803.721558E-410.7028775
GCCGGTT803.7961415E-410.6799211
TCCAACG1355.7751095E-810.5445918
GGACCTG13400.010.5041616
ATGTCCA5450.010.4599289
AGGACCT14350.010.4086845
TGTAGGA23550.010.3338482
Produced by FastQC (version 0.11.5)