##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513012_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1818950 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.48498419417796 31.0 31.0 34.0 27.0 34.0 2 30.614269771021743 31.0 31.0 34.0 27.0 34.0 3 30.70738832843124 31.0 31.0 34.0 27.0 34.0 4 34.190764452019025 37.0 35.0 37.0 30.0 37.0 5 33.87143296957036 37.0 35.0 37.0 30.0 37.0 6 33.762964897330875 37.0 35.0 37.0 29.0 37.0 7 33.63865911652327 37.0 35.0 37.0 28.0 37.0 8 33.523998460650375 37.0 35.0 37.0 28.0 37.0 9 34.98126666483411 39.0 35.0 39.0 28.0 39.0 10 34.85041919788889 39.0 35.0 39.0 27.0 39.0 11 34.902846697270405 39.0 35.0 39.0 27.0 39.0 12 34.74674290112428 39.0 35.0 39.0 27.0 39.0 13 34.7474515517194 39.0 35.0 39.0 27.0 39.0 14 35.792864564721405 40.0 36.0 41.0 27.0 41.0 15 35.81157206080431 40.0 36.0 41.0 27.0 41.0 16 35.79168421341983 40.0 36.0 41.0 27.0 41.0 17 35.771646279446934 40.0 36.0 41.0 27.0 41.0 18 35.70577915830562 40.0 36.0 41.0 27.0 41.0 19 35.68339811429671 40.0 36.0 41.0 27.0 41.0 20 35.667037576623876 40.0 36.0 41.0 27.0 41.0 21 35.60644382748289 40.0 36.0 41.0 27.0 41.0 22 35.58072624316226 40.0 36.0 41.0 27.0 41.0 23 35.462709805107345 40.0 36.0 41.0 26.0 41.0 24 35.443833530333436 40.0 36.0 41.0 26.0 41.0 25 35.34285549355398 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7244769136928717 1101 2 0.7399756107804301 1101 3 0.7302687270901238 1101 4 0.47418692693641873 1101 5 0.6648927164043172 1101 6 0.8044608573274274 1101 7 0.7609800825285618 1101 8 0.7621032898921598 1101 9 0.7279380549518848 1101 10 1.0183396661685507 1101 11 0.8714481304269981 1101 12 0.9644679361042279 1101 13 1.011402046153897 1101 14 1.2043982603749868 1101 15 1.059291164184451 1101 16 1.2042309203622352 1101 17 1.1694298106486585 1101 18 0.684773659584728 1101 19 1.0429026264441106 1101 20 0.9446372053317376 1101 21 1.1453587429898278 1101 22 1.0214970630497717 1101 23 1.0587465801143239 1101 24 1.115353113928407 1101 25 1.0852856468894387 1102 1 0.7543622944925694 1102 2 0.6685317810975953 1102 3 0.7057244402840901 1102 4 0.5901565582768526 1102 5 0.7656603929156276 1102 6 0.7660865492867401 1102 7 0.898263329710673 1102 8 0.9278721367065401 1102 9 1.0563628129247533 1102 10 1.1351232221414662 1102 11 1.0362965973299794 1102 12 0.9593034450732674 1102 13 1.1396929947761834 1102 14 1.2820263662606237 1102 15 1.2221307283241032 1102 16 1.1633503307550868 1102 17 1.2240441887383255 1102 18 1.2289698728788068 1102 19 1.2132204976769287 1102 20 1.2023936356535856 1102 21 1.1704811313104884 1102 22 1.207873818856875 1102 23 1.21989413982093 1102 24 1.1505602011709755 1102 25 1.1924540351706128 1103 1 0.511107603026101 1103 2 0.40075253018155976 1103 3 0.49233089983572853 1103 4 0.5955003331047024 1103 5 0.6104779335683119 1103 6 0.5210900884672043 1103 7 0.6371496873558016 1103 8 0.5501938071632324 1103 9 0.6951665288817992 1103 10 0.6892188657569349 1103 11 0.7414215221908051 1103 12 0.782112847435215 1103 13 0.8442349354328869 1103 14 1.027523830912152 1103 15 0.7342837768993604 1103 16 0.7880201288667337 1103 17 0.9228505818847239 1103 18 0.9672341374635636 1103 19 1.0238025104307482 1103 20 1.0146079585709629 1103 21 0.5912496670840284 1103 22 0.9431562326874072 1103 23 0.8911324379494516 1103 24 1.0074694476447945 1103 25 0.9868352897371153 1104 1 0.4810633864445464 1104 2 0.5455287032224234 1104 3 0.4998095026402716 1104 4 0.4450766631363692 1104 5 0.5271998005798295 1104 6 0.5410766000461251 1104 7 0.5099336292193257 1104 8 0.4780768948167662 1104 9 0.5897704689110412 1104 10 0.6736477771411415 1104 11 0.6053477501183693 1104 12 0.5189750425470692 1104 13 0.6953168943125831 1104 14 0.7689498465597637 1104 15 0.8032463622322155 1104 16 0.6892796263397258 1104 17 0.6860926278485167 1104 18 0.7099135400958829 1104 19 0.7065474324619387 1104 20 0.7747910192771315 1104 21 0.6404206189337245 1104 22 0.7372547707162411 1104 23 0.5985655465248882 1104 24 0.7139594550692081 1104 25 0.875455826033928 1105 1 0.62513848091195 1105 2 0.5889936878425424 1105 3 0.5861937140929996 1105 4 0.46221041314750977 1105 5 0.45246729237062766 1105 6 0.5004273422259757 1105 7 0.6768670669532639 1105 8 0.7393203227484975 1105 9 0.8271943884149309 1105 10 0.7939542178836305 1105 11 0.7454470442077366 1105 12 0.8109207288222677 1105 13 0.8064382540433073 1105 14 0.9725018276774406 1105 15 0.9143978288491468 1105 16 0.8467025580671148 1105 17 0.6591997363098727 1105 18 0.9450978229932616 1105 19 0.969380887369212 1105 20 1.0889741929556394 1105 21 1.0743737218768317 1105 22 1.012647871368074 1105 23 1.082645261891905 1105 24 1.04846353658197 1105 25 1.1982464118925051 1106 1 0.5990931056867943 1106 2 0.6223817928744353 1106 3 0.6111242572692746 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0.8595183892392484 1107 17 0.7441241071592444 1107 18 0.9280279387327397 1107 19 1.006355619322946 1107 20 0.928286602599151 1107 21 0.9043581980539912 1107 22 0.974197827327167 1107 23 1.0144005450539098 1107 24 1.076812949124097 1107 25 1.065488839135746 1108 1 0.38392857264367564 1108 2 0.3110773534846061 1108 3 0.26426098045269697 1108 4 0.31204680669901563 1108 5 0.34727329293388465 1108 6 0.34144053414747333 1108 7 0.28391767741491947 1108 8 0.33423450836895086 1108 9 0.3137229590745747 1108 10 0.4315846390571707 1108 11 0.4040435686622672 1108 12 0.4755611218261464 1108 13 0.4144012013312306 1108 14 0.5280352231137044 1108 15 0.503088358614221 1108 16 0.3993168106667184 1108 17 0.4600800464284589 1108 18 0.4813420505365471 1108 19 0.5266575496591912 1108 20 0.5595454676789089 1108 21 0.7230557022529922 1108 22 0.6532427730467489 1108 23 0.6049482680473304 1108 24 0.6276564948157031 1108 25 0.5718105980824788 1109 1 0.10653001972081455 1109 2 0.14965862240639893 1109 3 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44234.0 3 2.0 4 695.0 5 6251.0 6 7164.0 7 5623.0 8 4678.0 9 4027.0 10 3462.0 11 3217.0 12 2989.0 13 2834.0 14 2793.0 15 2758.0 16 2948.0 17 3056.0 18 3465.0 19 4095.0 20 5053.0 21 6823.0 22 8577.0 23 10565.0 24 12415.0 25 14388.0 26 16518.0 27 18174.0 28 20939.0 29 25061.0 30 31356.0 31 40267.0 32 51726.0 33 66492.0 34 86440.0 35 112843.0 36 156158.0 37 244178.0 38 786686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.20505374723121 19.787717968157693 13.385420850338528 27.621807434272565 2 13.32791628572324 21.15441389505698 40.20739448989499 25.310275329324796 3 16.89243794496825 25.332142169933203 30.330740262239203 27.44467962285934 4 11.411651684546777 17.773814806810666 37.96115591907841 32.853377589564154 5 12.286467490686318 38.58565175964211 36.42837652578233 12.699504223889235 6 31.309116677450167 38.12038125167804 18.02632273909656 12.54417933177523 7 27.152013418205318 31.791852839682694 23.022175783988892 18.0339579581231 8 24.532816064668854 37.915217304001146 20.666504195014394 16.885462436315603 9 26.18063213823108 14.879641826837137 20.191627042293806 38.74809899263798 10 14.399966096358991 29.26076853389869 35.16710202023871 21.17216334950361 11 33.628237332845124 22.35409701593878 25.354981617980567 18.66268403323553 12 23.09148552916052 24.974212236046565 32.21991409210575 19.714388142687163 13 28.815791137552825 22.170027869365306 25.90872329277458 23.105457700307284 14 20.361240363780517 22.688668119281566 27.74302021839807 29.207071298539855 15 22.598721576070403 31.841838748802783 23.21077280539608 22.348666869730735 16 21.53948685970877 28.244711357898826 28.478631801852778 21.737169980539623 17 21.278787315515633 29.099709136544146 28.207430904118446 21.414072643821775 18 21.22706341317523 27.579486378790147 30.41648707370495 20.77696313432967 19 23.234695655950727 27.140013161953046 27.826534915186773 21.798756266909454 20 23.438953533123236 27.597803879826966 28.20307843883258 20.76016414821722 21 22.311303016908674 26.963497582118894 27.56598869236504 23.159210708607393 22 22.883869495779148 28.465896439969324 27.270203332151326 21.3800307321002 23 21.45699441985761 28.355204925918798 28.14173011902471 22.04607053519888 24 22.83900052227934 27.333901426647238 28.510954121883504 21.316143929189916 25 22.194812463370678 27.644430858684764 28.198667544854082 21.962089133090476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 153.0 2 295.0 3 437.0 4 437.0 5 437.0 6 1076.0 7 1715.0 8 1715.0 9 1715.0 10 2461.5 11 3208.0 12 3208.0 13 3208.0 14 5725.0 15 8242.0 16 8242.0 17 8242.0 18 15351.0 19 22460.0 20 22460.0 21 22460.0 22 35988.0 23 49516.0 24 49516.0 25 49516.0 26 71332.5 27 93149.0 28 93149.0 29 93149.0 30 119306.0 31 145463.0 32 145463.0 33 145463.0 34 164461.0 35 183459.0 36 183459.0 37 183459.0 38 196314.0 39 209169.0 40 209169.0 41 209169.0 42 217971.0 43 226773.0 44 226773.0 45 226773.0 46 235137.0 47 243501.0 48 243501.0 49 243501.0 50 230823.0 51 218145.0 52 218145.0 53 218145.0 54 190261.0 55 162377.0 56 162377.0 57 162377.0 58 138171.5 59 113966.0 60 113966.0 61 113966.0 62 92384.5 63 70803.0 64 70803.0 65 70803.0 66 54665.0 67 38527.0 68 38527.0 69 38527.0 70 28004.5 71 17482.0 72 17482.0 73 17482.0 74 12151.5 75 6821.0 76 6821.0 77 6821.0 78 4668.5 79 2516.0 80 2516.0 81 2516.0 82 1658.5 83 801.0 84 801.0 85 801.0 86 509.5 87 218.0 88 218.0 89 218.0 90 132.5 91 47.0 92 47.0 93 47.0 94 24.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07520822452513813 2 5.4976772313697455E-5 3 0.0 4 0.02468457076885016 5 0.06564226614255478 6 0.07465845680200116 7 0.02886280546469117 8 0.06580719645949586 9 0.04590560488193739 10 0.11193270843068803 11 0.0029137689326259658 12 0.014184007256933947 13 0.005882514637565629 14 0.008851260342505291 15 0.008136562302427224 16 0.021056103796146128 17 0.03139173699112125 18 0.0012094889909013442 19 0.00390335083427252 20 0.0058275378652519316 21 1.0995354462739491E-4 22 2.748838615684873E-4 23 0.0 24 0.0 25 0.002858792160312268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1818950.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.98057366550145 #Duplication Level Percentage of deduplicated Percentage of total 1 83.68259644198078 59.39838701270447 2 11.186154347855815 15.879993054432983 3 2.751862635410546 5.859863655302977 4 0.9707792600551237 2.756258751251348 5 0.4310341668739427 1.529752621707197 6 0.24067046397102887 1.024975655820362 7 0.15815959246598496 0.7858381022756276 8 0.11110431430307044 0.6308998372755295 9 0.07293791569202446 0.4659457588007289 >10 0.3532697273002825 4.330359418781486 >50 0.020737292139126267 1.018586879297428 >100 0.01658684184257093 2.4799124175526845 >500 0.002479698179567826 1.2927413796309284 >1k 0.0016273019303413854 2.5464854551662803 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3716 0.20429368591769978 No Hit CTGTAGGACGTGGAATATGGCAAGA 3638 0.2000054976772314 No Hit CTTTAGGACGTGAAATATGGCGAGG 3611 0.19852112482476153 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3562 0.1958272629813904 No Hit GTATCAACGCAGAGTACTTTTTTTT 3480 0.19131916765166718 No Hit GTACATGGGAAGCAGTGGTATCAAC 3280 0.18032381318892768 No Hit CCCATGTACTCTGCGTTGATACCAC 3273 0.1799389757827318 No Hit TATCAACGCAGAGTACTTTTTTTTT 2513 0.13815662882432173 No Hit GGTATCAACGCAGAGTACTTTTTTT 2145 0.11792517661288107 No Hit GTCCTACAGTGTGCATTTCTCATTT 2030 0.11160284779680583 No Hit CTGTAGGACCTGGAATATGGCGAGA 1863 0.10242172682041836 No Hit CTGAAGGACCTGGAATATGGCGAGA 1861 0.10231177327579097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGTT 55 0.0030851366 12.0823345 12 TAGGACC 775 0.0 11.90743 4 GGACCGT 65 7.9503807E-4 11.705259 6 TGTACCG 75 2.0466674E-4 11.416402 5 AAATGTC 485 0.0 11.365894 7 TAAGACT 220 0.0 11.243427 4 GGCCCGC 60 0.00582808 11.095611 6 TCTTGCG 60 0.0058472296 11.090722 2 GGTTCCG 60 0.005856825 11.08828 7 ATTTAGA 580 0.0 10.981756 1 GGACCTA 130 3.2137905E-8 10.9736805 6 TAGAAAT 690 0.0 10.754582 4 GGCGAGG 830 0.0 10.748156 19 AGGACCG 80 3.721558E-4 10.702877 5 GCCGGTT 80 3.7961415E-4 10.67992 11 TCCAACG 135 5.7751095E-8 10.54459 18 GGACCTG 1340 0.0 10.504161 6 ATGTCCA 545 0.0 10.459928 9 AGGACCT 1435 0.0 10.408684 5 TGTAGGA 2355 0.0 10.333848 2 >>END_MODULE