FastQCFastQC Report
Thu 26 May 2016
SRR1513011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607212
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43850.1681873204020233No Hit
CTGTAGGACGTGGAATATGGCAAGA40220.15426440197421615No Hit
GTCCTAAAGTGTGTATTTCTCATTT35390.13573886588432393No Hit
GTATCAACGCAGAGTACTTTTTTTT35190.13497176294064311No Hit
CTTTAGGACGTGAAATATGGCGAGG32100.12312002246077419No Hit
GTCCTACAGTGTGCATTTCTCATTT28400.10892861800267872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACG550.003081568312.0845257
TAGGACC7900.011.5419724
GTAGGAC23850.010.8321933
GTATTAG2050.010.690641
TGTAGGA25450.010.6365622
CTAATAC2450.010.4672743
TGGCGAG21900.010.32647418
TTAGACT1752.3646862E-1010.3122024
TAGGACA7750.010.2946944
AGGACCT15050.010.2867615
TAGGACT4350.010.2622954
GTCCTAC21600.010.1903241
GTCCTAG3850.010.1473561
AGGACGT21700.010.1106665
CTGTAGG26700.010.0996171
GTTCTAG2650.010.067971
GACGTGG11700.010.0630617
GGACGTG21250.010.008836
GGCGAGG9600.09.98970619
CCTACAC3900.09.9851633
Produced by FastQC (version 0.11.5)