##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513011_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2607212
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.122250894825584	31.0	30.0	34.0	23.0	34.0
2	29.142309869699893	31.0	30.0	34.0	22.0	34.0
3	29.368737946895	31.0	30.0	34.0	23.0	34.0
4	32.82413896530087	35.0	33.0	37.0	26.0	37.0
5	32.94449933492175	35.0	35.0	37.0	26.0	37.0
6	32.938392428387104	37.0	35.0	37.0	26.0	37.0
7	32.82248317359693	37.0	35.0	37.0	25.0	37.0
8	32.808773126236	37.0	35.0	37.0	25.0	37.0
9	34.144513372905614	39.0	35.0	39.0	25.0	39.0
10	34.01928611865855	39.0	34.0	39.0	25.0	39.0
11	34.082914622976574	39.0	35.0	39.0	25.0	39.0
12	33.97344596450154	39.0	35.0	39.0	24.0	39.0
13	33.97448155347551	39.0	35.0	39.0	24.0	39.0
14	34.98078867387846	39.0	35.0	41.0	24.0	41.0
15	34.918653718991784	39.0	35.0	41.0	23.0	41.0
16	34.812057093937895	39.0	34.0	41.0	22.0	41.0
17	34.747344289608975	39.0	34.0	41.0	21.0	41.0
18	34.44541295452767	39.0	34.0	41.0	17.0	41.0
19	34.67748038901325	39.0	34.0	41.0	19.0	41.0
20	34.75187518314583	39.0	34.0	41.0	19.0	41.0
21	34.693230163101425	39.0	34.0	41.0	19.0	41.0
22	34.60294099597578	39.0	34.0	41.0	18.0	41.0
23	34.331789666509664	39.0	34.0	41.0	16.0	41.0
24	34.331190942662126	39.0	34.0	41.0	16.0	41.0
25	34.24363803173658	39.0	34.0	41.0	15.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5847174240792583
1101	2	0.8016394546018262
1101	3	0.8350488072863307
1101	4	0.8056172129419537
1101	5	0.5400844970239831
1101	6	0.7365364241848553
1101	7	0.885008520722522
1101	8	0.6535173676880106
1101	9	0.5989957738052922
1101	10	0.6797895861604957
1101	11	0.800559529318015
1101	12	1.0167938391056524
1101	13	0.7982210465494504
1101	14	0.7780418958306896
1101	15	0.5059759984430983
1101	16	1.0117983034986437
1101	17	1.0605498302583953
1101	18	1.3080855549350474
1101	19	0.7986630179933485
1101	20	0.9218337842872018
1101	21	0.9837845128908072
1101	22	0.9968165206937201
1101	23	1.2697732561803434
1101	24	0.8982966998791042
1101	25	1.1493210659411588
1102	1	0.7298730403361908
1102	2	0.6861713265871359
1102	3	0.7229890490383433
1102	4	0.8143013350169355
1102	5	0.653334202923304
1102	6	0.7589384930483121
1102	7	0.864683404649945
1102	8	0.8800064953690878
1102	9	0.854959307829418
1102	10	1.0286627227912675
1102	11	1.0638716924190987
1102	12	1.0097563045848759
1102	13	1.0483059532273487
1102	14	1.0521839245108353
1102	15	1.306428374199676
1102	16	1.1802532151681078
1102	17	0.9143775913558301
1102	18	1.31907156351852
1102	19	1.3556501352548622
1102	20	1.2037468433606762
1102	21	1.080414833981024
1102	22	1.3435449798570005
1102	23	1.5907302184025198
1102	24	1.4490434898548443
1102	25	1.2341201368589267
1103	1	0.5525023992279614
1103	2	0.7206910682696694
1103	3	0.720911590582503
1103	4	0.8204739815569724
1103	5	0.461996849588985
1103	6	0.728564502573235
1103	7	0.5177142754094533
1103	8	0.5937589425665948
1103	9	1.0284103053342193
1103	10	0.8985350955195486
1103	11	0.9309291013522127
1103	12	1.144217488950467
1103	13	0.9975630364805355
1103	14	1.2799191997918342
1103	15	1.2872222176629222
1103	16	1.35919064539722
1103	17	1.1893901152890933
1103	18	1.496721424755222
1103	19	1.4462706637493739
1103	20	1.3409210761745385
1103	21	1.1446913075546306
1103	22	1.2826572674425591
1103	23	1.583817820622123
1103	24	1.3361829567277113
1103	25	1.385232613585778
1104	1	0.7118022904025381
1104	2	0.6314168087169278
1104	3	0.6601251780580668
1104	4	0.8736457321294111
1104	5	0.6387399254807349
1104	6	0.66332974369341
1104	7	0.8671494666051842
1104	8	0.8539075833100114
1104	9	0.9912014954812136
1104	10	1.0642679236189636
1104	11	1.000852748485407
1104	12	0.9861713894434629
1104	13	1.015624663290815
1104	14	1.1245305126835419
1104	15	1.2258290543888748
1104	16	1.0423594335630852
1104	17	1.081909675955984
1104	18	1.3206209530664736
1104	19	1.2494462364917993
1104	20	1.2538487462690497
1104	21	1.3415587229828958
1104	22	1.1410117845909724
1104	23	1.4171883564100156
1104	24	1.1881070550868102
1104	25	0.9311325583486791
1105	1	0.501991476557393
1105	2	0.5971536342996799
1105	3	0.7133566672268437
1105	4	0.863068548454514
1105	5	0.5054936910046024
1105	6	0.3174519938759346
1105	7	0.6347456883168263
1105	8	0.6620178241476466
1105	9	0.562640972144365
1105	10	0.7696075912846254
1105	11	0.6583002013443959
1105	12	0.6277113229823215
1105	13	0.6913550100915771
1105	14	0.7733072314021499
1105	15	0.9706960856121682
1105	16	0.8467829900139421
1105	17	0.8405414372908169
1105	18	0.7804233645177518
1105	19	0.767271110581035
1105	20	0.7967527995123405
1105	21	0.7825034809975264
1105	22	0.5561408131812797
1105	23	0.7527701345853828
1105	24	0.7330251800157441
1105	25	0.7527209540349276
1106	1	0.5047347306879679
1106	2	0.5219564253232889
1106	3	0.6394838849404358
1106	4	0.7088194234069007
1106	5	0.7402064604398362
1106	6	0.6676424616178664
1106	7	0.5219167847196502
1106	8	0.6108821285543371
1106	9	0.7921839950664449
1106	10	0.8842073450095498
1106	11	0.6843024158017812
1106	12	0.7358912976319942
1106	13	0.7991316254122083
1106	14	1.0064515050843
1106	15	1.0047679508090681
1106	16	0.9445227344783262
1106	17	0.9259942405530808
1106	18	1.2529238802309877
1106	19	0.7808458094432282
1106	20	0.8399326063645276
1106	21	0.7811244907910364
1106	22	0.8198020509540669
1106	23	1.1928048886118816
1106	24	1.1028589243942335
1106	25	1.1819396936575899
1107	1	0.45209803363049517
1107	2	0.5427934350106653
1107	3	0.7215422514471612
1107	4	0.7068023908549961
1107	5	0.6481500335992365
1107	6	0.6371167413958432
1107	7	0.8327543374208162
1107	8	0.708339890977058
1107	9	0.8401492088032185
1107	10	0.7147329725077682
1107	11	0.7326024204321797
1107	12	0.9025722495600874
1107	13	0.8979636125987085
1107	14	1.1241972943079048
1107	15	1.259706609085157
1107	16	1.0912826720218902
1107	17	1.1808712954502667
1107	18	1.5361311294709026
1107	19	1.3360242109161007
1107	20	1.1884224571933686
1107	21	1.1775384671199234
1107	22	1.1444478011003838
1107	23	1.241921847929369
1107	24	1.218722883966194
1107	25	1.2587937626708765
1108	1	0.49587642791067665
1108	2	0.5463538398334506
1108	3	0.47519161467051774
1108	4	0.5737511384034661
1108	5	0.5300313796432761
1108	6	0.7185374691169315
1108	7	0.6508044106563418
1108	8	0.6729835815507741
1108	9	0.7604595494932056
1108	10	0.8276387676303685
1108	11	0.6858434025329174
1108	12	0.7917285308506905
1108	13	0.787601924292396
1108	14	0.9774454137686917
1108	15	0.9473291854859411
1108	16	0.9873425389566748
1108	17	1.0021947275067404
1108	18	1.128866641664409
1108	19	1.124816797164371
1108	20	1.0499468111071266
1108	21	0.9122676198221882
1108	22	0.8628867052983935
1108	23	1.088651695748439
1108	24	1.0597387061486927
1108	25	1.1942792686579793
1109	1	0.051438795341113064
1109	2	-0.03736934233913658
1109	3	0.17925233048329048
1109	4	0.2708024349652476
1109	5	0.1542480140321345
1109	6	0.06792691553157937
1109	7	0.21178117063521285
1109	8	0.19603444215256616
1109	9	0.42675365900300477
1109	10	0.5396861447441452
1109	11	0.09233836803339557
1109	12	0.3971747820139484
1109	13	0.4141859471970619
1109	14	0.21707721621174159
1109	15	0.4303676170360333
1109	16	0.41691456457770926
1109	17	0.275186901186494
1109	18	-1.3045158419661291
1109	19	0.024575866389788814
1109	20	0.31330594572654036
1109	21	0.4089088415162294
1109	22	0.5438630251253471
1109	23	0.12481436751248509
1109	24	0.6032372431675626
1109	25	0.6328455890718061
1110	1	0.4488094997930858
1110	2	0.36942826668803974
1110	3	0.5114674693642804
1110	4	0.3734648422669622
1110	5	0.45431778272021006
1110	6	0.383530425798682
1110	7	0.5495774535542068
1110	8	0.5282823417792599
1110	9	0.8215582610453538
1110	10	0.9656836567995128
1110	11	0.9022078702830072
1110	12	0.3124941045123677
1110	13	0.5610096641985081
1110	14	0.8116423372030255
1110	15	0.8705869783091345
1110	16	1.0450468136239621
1110	17	0.6317772633963159
1110	18	1.0557841481822408
1110	19	0.9204810539836643
1110	20	0.9172754482803569
1110	21	0.9769845595557243
1110	22	0.9558794794438796
1110	23	0.9683847318611143
1110	24	1.1400440579642819
1110	25	1.0151443614564641
1111	1	0.09457954188314233
1111	2	0.3861067847389883
1111	3	0.2506656101563216
1111	4	0.30396579498836473
1111	5	0.24352176901911093
1111	6	0.31494308514587743
1111	7	0.20126222244860514
1111	8	0.0506865097459297
1111	9	0.37576938343318744
1111	10	0.35507000847916004
1111	11	0.29771390234091655
1111	12	0.46928166411909444
1111	13	0.42487322188944887
1111	14	0.3202524921715053
1111	15	0.574739741850685
1111	16	0.37335002415434104
1111	17	0.4576480935713505
1111	18	0.592901913976597
1111	19	0.4642521131438073
1111	20	0.4110205153822051
1111	21	0.3963169858331739
1111	22	0.20778353904015034
1111	23	0.4801767790370022
1111	24	0.5765605303861179
1111	25	0.09393163047174369
1112	1	-0.24525075996381318
1112	2	0.0266861773357725
1112	3	0.06536073715773227
1112	4	0.2297094569681164
1112	5	0.09635646783339524
1112	6	0.09621692968033813
1112	7	-0.22514922412685934
1112	8	0.07320321292451837
1112	9	0.28754425420399343
1112	10	0.27433860053329795
1112	11	0.20479794875466695
1112	12	0.1394666019766504
1112	13	0.037423645223412905
1112	14	0.2119414642016224
1112	15	0.07306761949948282
1112	16	0.2064285264328305
1112	17	0.3150808230052462
1112	18	0.1740725170673798
1112	19	0.07954340779444635
1112	20	0.025541566959738304
1112	21	0.12123389659569739
1112	22	0.10925255504605502
1112	23	0.4189912066097463
1112	24	0.1923551255454541
1112	25	0.3774214530723867
1113	1	-0.13899676862907384
1113	2	-0.01253549804969012
1113	3	-0.21717903756846724
1113	4	0.06726691306821664
1113	5	-0.07389021099369586
1113	6	-0.014166898100498315
1113	7	-0.15065968476990577
1113	8	0.17456722169684724
1113	9	-0.08971013291819219
1113	10	-0.23074545171829186
1113	11	-0.1205914334345124
1113	12	0.08499193332913535
1113	13	-0.007044006323027929
1113	14	0.05769191161351728
1113	15	-0.32702688192306084
1113	16	-0.3282639400303964
1113	17	-0.3561597459726684
1113	18	-0.5012416033667222
1113	19	-0.3299872542604376
1113	20	-0.4699576653655768
1113	21	-0.4161370661811645
1113	22	-0.33806254338455943
1113	23	-0.6458009428411202
1113	24	-0.08503761988099967
1113	25	-0.07035420236692147
1114	1	-1.2112790464124075
1114	2	-1.0446928042088857
1114	3	-0.9689393630155543
1114	4	-1.086736824850398
1114	5	-1.1026521529030155
1114	6	-1.5387669667797041
1114	7	-1.6453099931530026
1114	8	-1.219573800084273
1114	9	-1.3791425411343
1114	10	-1.4966388200389034
1114	11	-2.1123684453312137
1114	12	-1.6273837779911986
1114	13	-1.570961296457078
1114	14	-1.8413667311930553
1114	15	-1.807880990555482
1114	16	-2.3766173104094577
1114	17	-4.358890261374508
1114	18	-3.3205798854824273
1114	19	-2.4125536177061733
1114	20	-1.632938576105694
1114	21	-2.5120636510932854
1114	22	-2.6092880487634673
1114	23	-2.4031039398130787
1114	24	-2.0147363018943594
1114	25	-1.8062223017420749
1115	1	-1.3007070923738944
1115	2	-0.8112073632374575
1115	3	-0.8861663586914581
1115	4	-0.864279759799782
1115	5	-0.7043294997637943
1115	6	-0.8140097306567
1115	7	-1.9091849208522333
1115	8	-1.2534642799869324
1115	9	-1.1255537911738145
1115	10	-0.8588804601960618
1115	11	-1.6391240434100212
1115	12	-1.2413807858172774
1115	13	-1.1342757374168144
1115	14	-1.0251937385842993
1115	15	-2.3012694020577626
1115	16	-1.1429677062598316
1115	17	-1.1756967729249084
1115	18	-1.121604525979599
1115	19	-1.2982273247711476
1115	20	-1.0070052846234603
1115	21	-1.728112908953868
1115	22	-1.7943827744430223
1115	23	-1.149313047702492
1115	24	-1.0272863727461683
1115	25	-1.2887590570673737
1116	1	-1.1697926719154452
1116	2	-1.8748576414831568
1116	3	-1.5238048735137255
1116	4	-1.373290784610532
1116	5	-1.7363356127129101
1116	6	-1.3854696344150454
1116	7	-1.1282855420501932
1116	8	-1.7748316638387784
1116	9	-1.8292489374582885
1116	10	-2.8402639782823442
1116	11	-1.4962421518431555
1116	12	-1.3929207346357373
1116	13	-2.072107361663768
1116	14	-1.807801393066228
1116	15	-1.4304663796576662
1116	16	-1.3711330457435267
1116	17	-1.7776878092950383
1116	18	-1.5482952842412274
1116	19	-1.9306010727652776
1116	20	-2.1698715009384415
1116	21	-2.586111066538038
1116	22	-1.8873275516082444
1116	23	-1.8588503683469568
1116	24	-2.505965635490483
1116	25	-2.1301960516097225
1201	1	0.4643125557235699
1201	2	0.42159722906779606
1201	3	0.6460108871008678
1201	4	0.6010924571876899
1201	5	0.7155771282543171
1201	6	0.6254749901725987
1201	7	0.49699312082186964
1201	8	0.1923979770604234
1201	9	0.4605208607402176
1201	10	0.5815779617193613
1201	11	0.25839195190453523
1201	12	-0.1862240446499399
1201	13	0.21473040240306318
1201	14	0.7273008791060676
1201	15	0.3583936172541655
1201	16	0.8980832844557582
1201	17	0.9756392490363339
1201	18	1.2849815642081026
1201	19	0.7438815972283237
1201	20	0.7223595335834432
1201	21	0.6608336932186774
1201	22	0.9697135235087586
1201	23	1.189813825811079
1201	24	1.0046263902251837
1201	25	0.9053733924852878
1202	1	0.7545703652557307
1202	2	0.8693310767052118
1202	3	0.6672160681300632
1202	4	0.9456944559603713
1202	5	0.8221403496680679
1202	6	1.0192755804534244
1202	7	0.9955700180487241
1202	8	1.0595482648953762
1202	9	1.2771374850352402
1202	10	1.4264696218111226
1202	11	1.1042285726929393
1202	12	1.2692893827776643
1202	13	1.2526402384686364
1202	14	1.4227462927409675
1202	15	1.149997091728899
1202	16	1.3983550474083302
1202	17	1.4403849461229186
1202	18	1.7154087872582764
1202	19	1.517892073251815
1202	20	1.4791050261172671
1202	21	1.5214404843703093
1202	22	1.569870590806616
1202	23	1.6203228699652499
1202	24	1.7313552263199838
1202	25	1.5462090635644543
1203	1	0.3988491042494857
1203	2	0.598291278512427
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2211	17	0.23999753309319516
2211	18	0.20348307855837788
2211	19	0.10875588211847287
2211	20	-0.025421021838575086
2211	21	0.013256653653265005
2211	22	0.006045911374727098
2211	23	0.5686898413872541
2211	24	0.23582521079470808
2211	25	0.25727153840776396
2212	1	-0.12097231396447938
2212	2	-0.09501290169364651
2212	3	-0.10794420866991672
2212	4	-0.11461521224720883
2212	5	-0.13975936335340577
2212	6	-0.21668650445263893
2212	7	-0.3165170085427391
2212	8	-0.2207399859493009
2212	9	0.08092210270350364
2212	10	0.01570315588805471
2212	11	-0.2635898957182192
2212	12	-0.04641703677646092
2212	13	-0.07770402973611823
2212	14	-0.13930733548753693
2212	15	-0.18345799542492358
2212	16	-0.028551663365384172
2212	17	-0.07689473070816177
2212	18	-2.6854924384086303
2212	19	-1.0095654454441956
2212	20	-0.3514679060116066
2212	21	-0.422514235224817
2212	22	-0.07075701567938353
2212	23	-0.10261774103040722
2212	24	-0.4692659125465255
2212	25	-0.1416047750207312
2213	1	-0.09853599777140687
2213	2	0.0928624169521548
2213	3	-0.2768724709000061
2213	4	-0.3378605322999917
2213	5	-0.23641271011005927
2213	6	-0.24377542308052114
2213	7	-0.020282588053959216
2213	8	-0.34854629823107786
2213	9	-0.27993926132595703
2213	10	-0.32380102861772286
2213	11	-0.3997483192686673
2213	12	-0.367259815214787
2213	13	-0.13143176382625654
2213	14	-0.18873920507261488
2213	15	-0.3619961995635208
2213	16	-0.86802668600793
2213	17	-0.33557995791097994
2213	18	-0.20321673523987727
2213	19	-0.10532715419819993
2213	20	-0.817002513284038
2213	21	-0.4754379055398772
2213	22	-0.3331656839442445
2213	23	-0.8959735281120516
2213	24	-0.5849054345708851
2213	25	-0.8307001941145273
2214	1	-1.8663119625600721
2214	2	-3.749224515700895
2214	3	-2.029255952251642
2214	4	-1.73422961515449
2214	5	-1.6760476082419018
2214	6	-1.629454080555007
2214	7	-1.8759971018797224
2214	8	-1.5215790604581372
2214	9	-1.7919265193466245
2214	10	-1.7874331279099849
2214	11	-1.5031912019826947
2214	12	-2.0825324582088562
2214	13	-1.8286535424983867
2214	14	-3.2013764547691075
2214	15	-3.3408016188368244
2214	16	-2.483513476526106
2214	17	-2.952888111434291
2214	18	-2.336692699158945
2214	19	-4.531463335236541
2214	20	-2.834224362532094
2214	21	-2.799700257933793
2214	22	-2.758115811340094
2214	23	-2.941605653366171
2214	24	-3.1437052002083945
2214	25	-2.914470100464051
2215	1	-1.102306929040985
2215	2	-2.5773755816049935
2215	3	-1.402758489425075
2215	4	-0.9924490662887102
2215	5	-1.0214117439243147
2215	6	-1.5265879704463892
2215	7	-1.9692313667365475
2215	8	-1.9057649532381475
2215	9	-1.6277350042953458
2215	10	-3.2049435903535013
2215	11	-2.1969659538239092
2215	12	-2.3779746217586997
2215	13	-1.7522747792888467
2215	14	-3.0729370719291005
2215	15	-2.9845341256286666
2215	16	-2.622733083430006
2215	17	-2.843016656527272
2215	18	-1.7291117606869264
2215	19	-2.261628250859566
2215	20	-2.5310454648249276
2215	21	-2.2239642071866825
2215	22	-1.882954938187325
2215	23	-2.428954892890271
2215	24	-2.277738768964589
2215	25	-2.217534706394062
2216	1	-2.2943537586622824
2216	2	-2.304717046699274
2216	3	-2.75275723093522
2216	4	-3.755887725640548
2216	5	-2.627944826684015
2216	6	-2.589877297519724
2216	7	-2.532944555748564
2216	8	-3.217315615887358
2216	9	-3.4647371230300976
2216	10	-2.7837124704163543
2216	11	-3.494234673093917
2216	12	-4.014153445373491
2216	13	-4.567813257712004
2216	14	-3.834452158283863
2216	15	-3.638956708123956
2216	16	-2.9000549940194738
2216	17	-3.0577180283834267
2216	18	-3.0903454308228113
2216	19	-3.3325612747960385
2216	20	-3.217471316582998
2216	21	-3.577627390072344
2216	22	-4.138586680291194
2216	23	-4.145861624482237
2216	24	-4.046172227623067
2216	25	-4.050676135995101
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121481.0
3	3.0
4	993.0
5	5793.0
6	7350.0
7	6815.0
8	5904.0
9	5691.0
10	5214.0
11	4977.0
12	4898.0
13	4746.0
14	4891.0
15	4801.0
16	4974.0
17	5487.0
18	6011.0
19	7185.0
20	9256.0
21	11803.0
22	15040.0
23	18075.0
24	21011.0
25	23600.0
26	26673.0
27	29645.0
28	33719.0
29	39978.0
30	49060.0
31	61100.0
32	76981.0
33	98819.0
34	127855.0
35	167406.0
36	230156.0
37	354016.0
38	1005805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70931080740368	18.163205210372244	12.360391778728548	25.767092203495523
2	15.154084818979019	19.514174483397426	40.778048333142756	24.553692364480796
3	19.1182767644057	23.673770076661576	29.21815168095278	27.989801477979942
4	12.639495064795542	15.927904768299584	37.509587438010186	33.92301272889468
5	13.552352177820207	37.46670360510456	35.44666475835125	13.534279458723983
6	33.06835601535084	36.23308439122222	17.445230945340658	13.253328648086285
7	28.935562140959615	30.98433888617161	21.57155872677926	18.508540246089517
8	25.59054831134622	36.838291063119314	19.812760132632395	17.758400492902066
9	26.490067495455932	15.144009998872136	19.633873404396525	38.73204910127541
10	15.184760495937583	28.446590879882947	34.14644345204414	22.222205172135332
11	35.10205537836409	21.94978981264592	24.10149605519883	18.84665875379116
12	23.683606820255143	24.172192798113787	31.10592448860746	21.03827589302361
13	29.272211263358756	21.38060196768504	25.37201952800418	23.975167240952032
14	21.2870548308101	21.471707943460007	26.942550361422118	30.29868686430778
15	23.34830494201203	30.425948469377772	22.737982013352063	23.487764575258137
16	22.886713106433714	27.61407832356482	26.901637872630026	22.597570697371438
17	22.24514669724514	28.175300877340714	27.235849241184106	22.34370318423004
18	22.325079773152837	27.037714051401128	28.809098458749283	21.82810771669675
19	23.92608404416205	26.624368617599597	26.892727717754518	22.556819620483836
20	24.063763483390257	26.808875664506665	27.3279744405906	21.799386411512476
21	23.38700333698312	26.34298416371704	26.643535161090576	23.626477338209263
22	23.737559543287592	27.438191410881963	26.469846937738158	22.354402108092287
23	22.81452535484351	27.27144732571638	27.06992367958469	22.844103639855415
24	23.572203484500204	26.391122411713617	27.55529503472531	22.481379069060868
25	23.201590095433346	26.823538077430065	27.2250901161497	22.74978171098688
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	187.0
2	397.5
3	608.0
4	608.0
5	608.0
6	1102.0
7	1596.0
8	1596.0
9	1596.0
10	2573.0
11	3550.0
12	3550.0
13	3550.0
14	6320.5
15	9091.0
16	9091.0
17	9091.0
18	16823.0
19	24555.0
20	24555.0
21	24555.0
22	39616.0
23	54677.0
24	54677.0
25	54677.0
26	79856.0
27	105035.0
28	105035.0
29	105035.0
30	137788.5
31	170542.0
32	170542.0
33	170542.0
34	198372.5
35	226203.0
36	226203.0
37	226203.0
38	251431.5
39	276660.0
40	276660.0
41	276660.0
42	296119.5
43	315579.0
44	315579.0
45	315579.0
46	330049.5
47	344520.0
48	344520.0
49	344520.0
50	338019.5
51	331519.0
52	331519.0
53	331519.0
54	301828.0
55	272137.0
56	272137.0
57	272137.0
58	238261.0
59	204385.0
60	204385.0
61	204385.0
62	168897.0
63	133409.0
64	133409.0
65	133409.0
66	104056.5
67	74704.0
68	74704.0
69	74704.0
70	55190.5
71	35677.0
72	35677.0
73	35677.0
74	25015.0
75	14353.0
76	14353.0
77	14353.0
78	9995.5
79	5638.0
80	5638.0
81	5638.0
82	3741.0
83	1844.0
84	1844.0
85	1844.0
86	1197.0
87	550.0
88	550.0
89	550.0
90	347.5
91	145.0
92	145.0
93	145.0
94	89.5
95	34.0
96	34.0
97	34.0
98	24.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35735490631371747
2	0.04027290454324389
3	0.0365141001192078
4	0.035938772911447175
5	0.0411934280756609
6	0.017183105938450727
7	0.016761199319426268
8	0.023358284635081456
9	0.019867966241333655
10	0.019676190505413443
11	0.018794022120180484
12	0.019407704475125152
13	0.018947442708916653
14	0.02696366847038139
15	0.027730771414062227
16	0.020635069185014492
17	0.022437761102664457
18	0.10121923341868631
19	0.024163742725946336
20	0.022207630219560206
21	0.02554452802457184
22	0.018985797856100693
23	0.021862433894903825
24	0.017950208882131564
25	0.02209256477800808
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2607212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.5934451810574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93480214595327	54.030686669111624
2	12.75136221394325	17.238170055839596
3	3.75392747895336	7.612226737868971
4	1.4734055502518086	3.9837022916164546
5	0.6819716279919611	2.3048405925855553
6	0.37209392633725713	1.5090666247249085
7	0.23617890198547167	1.117490196499412
8	0.16302593620326372	0.8815587745476696
9	0.10713376264609474	0.65173861012133
>10	0.4880456757409447	5.493966772901693
>50	0.02219458750496069	1.0342394934884245
>100	0.012785143141093634	1.687586570034178
>500	0.0021056078861910383	0.9918918809642319
>1k	9.674414611805769E-4	1.4628347296959217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4385	0.1681873204020233	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4022	0.15426440197421615	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3539	0.13573886588432393	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3519	0.13497176294064311	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3210	0.12312002246077419	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2840	0.10892861800267872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.1506544155212541E-4	0.0	0.0	0.0	0.0
7	1.1506544155212541E-4	0.0	0.0	0.0	0.0
8	1.1506544155212541E-4	0.0	0.0	0.0	0.0
9	1.1506544155212541E-4	0.0	0.0	0.0	0.0
10	1.1506544155212541E-4	0.0	0.0	0.0	0.0
11	1.1506544155212541E-4	0.0	0.0	0.0	0.0
12	1.1506544155212541E-4	0.0	0.0	0.0	0.0
13	1.1506544155212541E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTACG	55	0.0030815683	12.084525	7
TAGGACC	790	0.0	11.541972	4
GTAGGAC	2385	0.0	10.832193	3
GTATTAG	205	0.0	10.69064	1
TGTAGGA	2545	0.0	10.636562	2
CTAATAC	245	0.0	10.467274	3
TGGCGAG	2190	0.0	10.326474	18
TTAGACT	175	2.3646862E-10	10.312202	4
TAGGACA	775	0.0	10.294694	4
AGGACCT	1505	0.0	10.286761	5
TAGGACT	435	0.0	10.262295	4
GTCCTAC	2160	0.0	10.190324	1
GTCCTAG	385	0.0	10.147356	1
AGGACGT	2170	0.0	10.110666	5
CTGTAGG	2670	0.0	10.099617	1
GTTCTAG	265	0.0	10.06797	1
GACGTGG	1170	0.0	10.063061	7
GGACGTG	2125	0.0	10.00883	6
GGCGAGG	960	0.0	9.989706	19
CCTACAC	390	0.0	9.985163	3
>>END_MODULE