FastQCFastQC Report
Thu 26 May 2016
SRR1513011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607212
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43160.1655408152463244No Hit
GTCCTACAGTGGACATTTCTAAATT42160.16170530052792023No Hit
CTGTAGGACGTGGAATATGGCAAGA40420.15503150491789697No Hit
CTTTAGGACGTGAAATATGGCGAGG33950.13021572468982193No Hit
GTCCTAAAGTGTGTATTTCTCATTT33470.12837467762498792No Hit
TATCAACGCAGAGTACTTTTTTTTT30060.11529557243522967No Hit
GTCCTACAGTGTGCATTTCTCATTT26590.10198633636236715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7000.012.7790944
GACCCGT604.0432488E-412.6852916
ACTGCGC951.0352487E-612.0051798
GGCGAGG9350.011.36756119
CCTTACG600.00583937811.0930192
TAGGACT3200.011.0032764
TGGCGAG20450.010.81243518
GTCCTAC21100.010.68178751
CGTGCGC909.51524E-510.55605710
TTAGGAC13050.010.2006723
AACCGTC856.5642124E-410.0641337
TCCTACA24700.010.0472342
TGTAGGA22500.010.015412
GTAGGAC21550.09.9717743
GTCCTAA11750.09.9550921
GCGTGCG1151.0252432E-59.9144669
CTATTAG1451.5937621E-79.8378751
AGGACCT16350.09.720065
ATAACAC2550.09.6949243
GTATCAA27000.09.6860561
Produced by FastQC (version 0.11.5)