##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513011_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2607212 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.50802543099679 31.0 31.0 34.0 27.0 34.0 2 30.671639667200058 31.0 31.0 34.0 27.0 34.0 3 30.773144262913796 31.0 31.0 34.0 27.0 34.0 4 34.248135939846854 37.0 35.0 37.0 30.0 37.0 5 33.903115281764585 37.0 35.0 37.0 30.0 37.0 6 33.76382971542015 37.0 35.0 37.0 29.0 37.0 7 33.645198012282854 37.0 35.0 37.0 28.0 37.0 8 33.53500789348929 37.0 35.0 37.0 28.0 37.0 9 35.00697411641247 39.0 35.0 39.0 28.0 39.0 10 34.901839973120715 39.0 35.0 39.0 27.0 39.0 11 34.92034287967377 39.0 35.0 39.0 27.0 39.0 12 34.76505861433593 39.0 35.0 39.0 27.0 39.0 13 34.7443978472023 39.0 35.0 39.0 27.0 39.0 14 35.814289747055476 40.0 36.0 41.0 27.0 41.0 15 35.82418805988926 40.0 36.0 41.0 27.0 41.0 16 35.80114390390962 40.0 36.0 41.0 27.0 41.0 17 35.7744667483887 40.0 36.0 41.0 27.0 41.0 18 35.69875330429593 40.0 36.0 41.0 27.0 41.0 19 35.68324670184089 40.0 36.0 41.0 27.0 41.0 20 35.652981805852384 40.0 36.0 41.0 27.0 41.0 21 35.60139796840456 40.0 36.0 41.0 27.0 41.0 22 35.562288759026885 40.0 36.0 41.0 27.0 41.0 23 35.45213546117462 40.0 36.0 41.0 26.0 41.0 24 35.433824713908955 40.0 36.0 41.0 26.0 41.0 25 35.33098727683058 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5354214072038701 1101 2 0.5333480186892601 1101 3 0.5183753956881461 1101 4 0.4067579258595799 1101 5 0.5923181471007695 1101 6 0.5849061174763861 1101 7 0.6361619025928178 1101 8 0.5504929738679252 1101 9 0.4847236558879757 1101 10 0.8276578444559703 1101 11 0.7817487318834466 1101 12 0.8744177340774613 1101 13 0.9353513888603473 1101 14 0.9620869932493648 1101 15 0.9093312401845068 1101 16 1.0085180658921757 1101 17 0.9428858893505563 1101 18 0.48155578573027924 1101 19 0.8008971747832234 1101 20 0.7718334587807192 1101 21 0.8685583495350926 1101 22 0.7747734004598499 1101 23 0.8186126638219946 1101 24 0.7286146764512011 1101 25 0.8076501279953376 1102 1 0.6234217859603923 1102 2 0.5534599901545931 1102 3 0.5484859750351312 1102 4 0.4882783072247605 1102 5 0.5983428214579476 1102 6 0.659123019549007 1102 7 0.668464942929134 1102 8 0.6868547916985719 1102 9 0.7656553969389179 1102 10 0.885559809224091 1102 11 0.7460065321357803 1102 12 0.768242685854986 1102 13 0.9704604255896996 1102 14 0.9993457867969582 1102 15 0.9433642697601243 1102 16 0.9053693073858327 1102 17 0.9191645061618274 1102 18 0.9524670337957346 1102 19 1.0154125796388342 1102 20 1.074085241570124 1102 21 0.9997205426106177 1102 22 1.0884034305847905 1102 23 1.2172596752825129 1102 24 1.1159664033050376 1102 25 1.1882075647919095 1103 1 0.5341680399947464 1103 2 0.43352134817082444 1103 3 0.5196940700879757 1103 4 0.4909836467694859 1103 5 0.5541828834399567 1103 6 0.6694278811902734 1103 7 0.6833424590742823 1103 8 0.7349273321167544 1103 9 0.8440704852176992 1103 10 0.9248293887920269 1103 11 0.921380567225377 1103 12 0.8743729819855304 1103 13 0.9176945590121264 1103 14 1.0628958024363158 1103 15 0.8336160091649489 1103 16 0.887185885619644 1103 17 1.0727015848042427 1103 18 1.0284246207438201 1103 19 1.1257924240245956 1103 20 1.1518339649423126 1103 21 0.7729239932615286 1103 22 1.0187142141207843 1103 23 1.0242955004996972 1103 24 1.0386084667126525 1103 25 1.1357716635661461 1104 1 0.6017497011811699 1104 2 0.6261948088870426 1104 3 0.6031240968275782 1104 4 0.5352620621451791 1104 5 0.5689748911416146 1104 6 0.6694318382603797 1104 7 0.5579102915597502 1104 8 0.5425821883081312 1104 9 0.8185274777712692 1104 10 0.829799570769886 1104 11 0.8026074292322107 1104 12 0.7846263321999629 1104 13 0.8519784765139278 1104 14 0.9594541527026124 1104 15 1.0359606691318177 1104 16 0.9574472027352243 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0.09406602950219423 2212 7 0.12817176327607882 2212 8 -0.14756347381000978 2212 9 -0.20142477871034714 2212 10 -0.15755877742250846 2212 11 -0.05526196630245295 2212 12 0.02493425472736277 2212 13 -0.05764253742485437 2212 14 -0.19962329638089926 2212 15 -0.10913714955280795 2212 16 0.049580274131749036 2212 17 -0.1706872974562259 2212 18 -0.06420754330630274 2212 19 0.06707372033972803 2212 20 -0.3155244388691756 2212 21 -0.0010474052004383338 2212 22 -0.12357690140522948 2212 23 -0.5471516847830173 2212 24 -0.3118572744929935 2212 25 -0.514023894129302 2213 1 -0.29963837428448414 2213 2 -0.40527325676506365 2213 3 -0.29955737435305707 2213 4 -0.3019744060655185 2213 5 -0.322922593226032 2213 6 -0.0819088852680494 2213 7 -0.6471448556709092 2213 8 -0.3266587374920533 2213 9 -0.4659040869944988 2213 10 -0.3825659822050298 2213 11 -0.2752844720516521 2213 12 -0.43479717130784223 2213 13 -0.39960122307160617 2213 14 -0.2879693235453402 2213 15 -0.5211028027280236 2213 16 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2216 14 -2.474460615805171 2216 15 -2.42052829701575 2216 16 -2.2553358471999587 2216 17 -2.5244530121296194 2216 18 -2.285734849150387 2216 19 -2.527805363060587 2216 20 -2.9369475647510015 2216 21 -2.830020933600977 2216 22 -3.1306947156038305 2216 23 -2.1845939172239213 2216 24 -2.4087975342989623 2216 25 -2.296663786775646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62986.0 3 4.0 4 1054.0 5 8943.0 6 10645.0 7 8261.0 8 6722.0 9 5991.0 10 5260.0 11 4786.0 12 4367.0 13 4208.0 14 4038.0 15 4063.0 16 4289.0 17 4478.0 18 4893.0 19 5858.0 20 7280.0 21 9651.0 22 12094.0 23 15030.0 24 17987.0 25 20829.0 26 23667.0 27 26122.0 28 29523.0 29 35585.0 30 44562.0 31 57025.0 32 72825.0 33 93495.0 34 122696.0 35 160115.0 36 220387.0 37 342337.0 38 1145156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.99607699078323 19.008630236420803 13.43788037739195 27.557412395404015 2 14.072179895121526 19.87927315350367 39.571066059938445 26.477480891436358 3 17.417379177450858 23.743447023103606 29.741156453713774 29.098017345731762 4 11.766520329127104 16.53350756235011 36.77477843156287 34.925193676959914 5 13.173502846174543 37.651141252470325 35.62249105424946 13.552864847105676 6 33.201724401992486 36.65459718339486 17.082880231906042 13.060798182706618 7 28.68621403163731 31.13277441697268 21.506334713678463 18.674676837711544 8 25.693914613309836 36.94100363363949 19.623917545397468 17.74116420765321 9 26.27329097215827 14.859601866347424 19.47459096908862 39.39251619240569 10 14.87536671247093 28.734439316260975 34.1108923922304 22.279301579037696 11 35.12379513104448 21.916053284646335 23.94391533985647 19.01623624445271 12 23.406938804631597 24.316042964953073 31.098573742156578 21.178444488258748 13 29.16014922293148 21.29790656766545 25.43456230778495 24.107381901618115 14 21.072266401132918 21.36573694054182 26.93761133162209 30.624385326703173 15 23.189415308892762 30.51901625032699 22.718966693081853 23.57260174769839 16 22.785442339966437 27.736312634859743 26.799980819947255 22.678264205226565 17 22.151614887030608 28.313993257860552 27.15324345092882 22.381148404180017 18 22.19549269001418 27.19394504498527 28.724483514224335 21.88607875077622 19 23.796082781390908 26.63066575226032 26.952472349180486 22.620779117168286 20 23.995941882230202 26.778706005093746 27.331461536101138 21.89389057657492 21 23.26548302591659 26.297766578066206 26.724122721223072 23.712627674794128 22 23.600213408939318 27.37399226219996 26.61751050264249 22.40828382621823 23 22.616572798836458 27.15697841218896 27.140524054047006 23.085924734927577 24 23.47029066692339 26.43345705430055 27.571377997407957 22.5248742813681 25 23.060450637323793 26.76176916420677 27.289241287044252 22.888538911425186 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 81.0 1 81.0 2 232.5 3 384.0 4 384.0 5 384.0 6 1024.0 7 1664.0 8 1664.0 9 1664.0 10 2568.5 11 3473.0 12 3473.0 13 3473.0 14 6143.5 15 8814.0 16 8814.0 17 8814.0 18 16596.5 19 24379.0 20 24379.0 21 24379.0 22 40006.0 23 55633.0 24 55633.0 25 55633.0 26 80765.0 27 105897.0 28 105897.0 29 105897.0 30 138163.0 31 170429.0 32 170429.0 33 170429.0 34 198835.0 35 227241.0 36 227241.0 37 227241.0 38 251968.0 39 276695.0 40 276695.0 41 276695.0 42 297144.5 43 317594.0 44 317594.0 45 317594.0 46 331641.0 47 345688.0 48 345688.0 49 345688.0 50 339645.5 51 333603.0 52 333603.0 53 333603.0 54 303454.5 55 273306.0 56 273306.0 57 273306.0 58 237752.5 59 202199.0 60 202199.0 61 202199.0 62 166758.0 63 131317.0 64 131317.0 65 131317.0 66 102228.5 67 73140.0 68 73140.0 69 73140.0 70 53747.0 71 34354.0 72 34354.0 73 34354.0 74 24111.0 75 13868.0 76 13868.0 77 13868.0 78 9573.5 79 5279.0 80 5279.0 81 5279.0 82 3461.0 83 1643.0 84 1643.0 85 1643.0 86 1034.0 87 425.0 88 425.0 89 425.0 90 258.0 91 91.0 92 91.0 93 91.0 94 52.5 95 14.0 96 14.0 97 14.0 98 7.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0793567995237825 2 1.5342058873616722E-4 3 0.0 4 0.02611985523233247 5 0.06846393772351463 6 0.0774390421645804 7 0.032947071431091914 8 0.05983402960710522 9 0.04034961483761198 10 0.10167949518489483 11 0.003145122069091428 12 0.00958878679601045 13 0.004065645601508431 14 0.005216300017029686 15 0.004832748545189267 16 0.012925684601022088 17 0.02381854640128996 18 9.588786796010451E-4 19 0.002147888242306341 20 0.0035286735409318457 21 7.671029436808361E-5 22 4.2190661902445983E-4 23 0.0 24 3.8355147184041804E-5 25 0.002301308831042508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2607212.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.43775884133831 #Duplication Level Percentage of deduplicated Percentage of total 1 79.07388439662144 52.53491662190599 2 13.237142976596447 17.588922256548596 3 3.9139034388524188 7.800929183962851 4 1.5350455963392655 4.079399565601859 5 0.7437011333568584 2.470491827399647 6 0.40266669643092784 1.605136372254982 7 0.2552500181013607 1.187076740280581 8 0.17023460051158917 0.9048004268192423 9 0.11620012085167174 0.694806804582996 >10 0.511248835085229 5.6875172102955345 >50 0.024087228239971514 1.1035150077192568 >100 0.013685850886076672 1.8165001699471617 >500 0.0018504207854726515 0.8801963008054872 >1k 0.0010986873413089773 1.6457915118757784 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4316 0.1655408152463244 No Hit GTCCTACAGTGGACATTTCTAAATT 4216 0.16170530052792023 No Hit CTGTAGGACGTGGAATATGGCAAGA 4042 0.15503150491789697 No Hit CTTTAGGACGTGAAATATGGCGAGG 3395 0.13021572468982193 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3347 0.12837467762498792 No Hit TATCAACGCAGAGTACTTTTTTTTT 3006 0.11529557243522967 No Hit GTCCTACAGTGTGCATTTCTCATTT 2659 0.10198633636236715 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.1506544155212541E-4 0.0 0.0 0.0 0.0 7 1.1506544155212541E-4 0.0 0.0 0.0 0.0 8 1.1506544155212541E-4 0.0 0.0 0.0 0.0 9 1.1506544155212541E-4 0.0 0.0 0.0 0.0 10 1.1506544155212541E-4 0.0 0.0 0.0 0.0 11 1.1506544155212541E-4 0.0 0.0 0.0 0.0 12 1.1506544155212541E-4 0.0 0.0 0.0 0.0 13 1.1506544155212541E-4 0.0 0.0 0.0 3.8355147184041804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 700 0.0 12.779094 4 GACCCGT 60 4.0432488E-4 12.685291 6 ACTGCGC 95 1.0352487E-6 12.005179 8 GGCGAGG 935 0.0 11.367561 19 CCTTACG 60 0.005839378 11.093019 2 TAGGACT 320 0.0 11.003276 4 TGGCGAG 2045 0.0 10.812435 18 GTCCTAC 2110 0.0 10.6817875 1 CGTGCGC 90 9.51524E-5 10.556057 10 TTAGGAC 1305 0.0 10.200672 3 AACCGTC 85 6.5642124E-4 10.064133 7 TCCTACA 2470 0.0 10.047234 2 TGTAGGA 2250 0.0 10.01541 2 GTAGGAC 2155 0.0 9.971774 3 GTCCTAA 1175 0.0 9.955092 1 GCGTGCG 115 1.0252432E-5 9.914466 9 CTATTAG 145 1.5937621E-7 9.837875 1 AGGACCT 1635 0.0 9.72006 5 ATAACAC 255 0.0 9.694924 3 GTATCAA 2700 0.0 9.686056 1 >>END_MODULE