FastQCFastQC Report
Thu 26 May 2016
SRR1513010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068439
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41910.2026165625382233No Hit
GTCCTAAAGTGTGTATTTCTCATTT38250.18492205958212932No Hit
CTGTAGGACGTGGAATATGGCAAGA35870.17341579809701907No Hit
CTTTAGGACGTGAAATATGGCGAGG33480.16186119097541674No Hit
GTCCTACAGTGTGCATTTCTCATTT24950.1206223630476896No Hit
GTATCAACGCAGAGTACTTTTTTTT24540.11864019195151512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG508.7310305E-515.1969615
TAGGACC7600.013.4973024
TCCAACG1850.012.32573718
CCAACGT853.9667684E-612.28809319
CCAACGA951.0464883E-611.99412119
GGCGAGG9300.011.84365719
TCAATAC2250.011.3977213
TGCACCG600.00588551911.0811195
TGGCGAG21250.010.95419718
GGTATCA9450.010.6870421
GTCCTAC21350.010.620931
TCCTACA23450.010.53119852
AGGACCT15250.010.5257645
TGTAGGA21750.010.5245882
GTCCTAA12600.010.5106061
TAAGACT2001.8189894E-1210.4479114
GATATAC4200.010.4349891
GTAGGAC21450.010.3615653
CGGTTTC1303.8326834E-710.23268513
GGACCTG14950.010.1622426
Produced by FastQC (version 0.11.5)