##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513010_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2068439
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.148698607984088	31.0	30.0	34.0	23.0	34.0
2	29.184553182375694	31.0	30.0	34.0	23.0	34.0
3	29.422209211874268	31.0	30.0	34.0	23.0	34.0
4	32.8828604566052	35.0	35.0	37.0	26.0	37.0
5	33.01230783213815	37.0	35.0	37.0	26.0	37.0
6	32.99982402188317	37.0	35.0	37.0	26.0	37.0
7	32.89068906552236	37.0	35.0	37.0	26.0	37.0
8	32.88148405633427	37.0	35.0	37.0	26.0	37.0
9	34.237786562717105	39.0	35.0	39.0	25.0	39.0
10	34.123701013179506	39.0	35.0	39.0	25.0	39.0
11	34.16374715425497	39.0	35.0	39.0	25.0	39.0
12	34.077093402319335	39.0	35.0	39.0	25.0	39.0
13	34.07420475053893	39.0	35.0	39.0	25.0	39.0
14	35.07644702115943	39.0	35.0	41.0	25.0	41.0
15	35.01830172415043	39.0	35.0	41.0	24.0	41.0
16	34.92971172947329	39.0	35.0	41.0	23.0	41.0
17	34.850486284584655	39.0	34.0	41.0	22.0	41.0
18	34.563306918889076	39.0	34.0	41.0	18.0	41.0
19	34.78644136955453	39.0	34.0	41.0	21.0	41.0
20	34.87045738356316	39.0	35.0	41.0	22.0	41.0
21	34.80814856033946	39.0	35.0	41.0	22.0	41.0
22	34.71593264292542	39.0	35.0	41.0	19.0	41.0
23	34.46000583048376	39.0	34.0	41.0	18.0	41.0
24	34.46434823555348	39.0	34.0	41.0	18.0	41.0
25	34.36878486626872	39.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.49734641405119007
1101	2	0.7151343173634821
1101	3	0.6678880158229177
1101	4	0.6560378456646418
1101	5	0.4991767112420078
1101	6	0.6742825100730272
1101	7	0.6269319375327029
1101	8	0.33772889791808325
1101	9	0.32322537172527177
1101	10	0.43725912011129964
1101	11	0.605764716289336
1101	12	0.6408705552396086
1101	13	0.5695451567499035
1101	14	0.6315666074462385
1101	15	0.16489290242714105
1101	16	0.8079632632505422
1101	17	0.7913721336508743
1101	18	1.0770643644393516
1101	19	0.4830696793675955
1101	20	0.7287248372395183
1101	21	0.7420176083633905
1101	22	0.7128759967556562
1101	23	0.9638084478038422
1101	24	0.6096906382858123
1101	25	0.7832566377065646
1102	1	0.3687584262173722
1102	2	0.5041643906535782
1102	3	0.4753063424073254
1102	4	0.5361594963208987
1102	5	0.4245224281483786
1102	6	0.36448591798966135
1102	7	0.5565212690198678
1102	8	0.5163399395843484
1102	9	0.4566736629963941
1102	10	0.6521583711938703
1102	11	0.537440087484832
1102	12	0.6021067646302853
1102	13	0.5819526987115466
1102	14	0.5881650156309064
1102	15	0.7581442378818153
1102	16	0.7337130144800383
1102	17	0.6578490556172483
1102	18	1.0176973317263176
1102	19	0.9201907810611445
1102	20	0.8633895979121249
1102	21	0.5625041087953448
1102	22	0.9363733100528293
1102	23	1.001301606673131
1102	24	0.8758047933575099
1102	25	0.7732623391520477
1103	1	0.43680291463709153
1103	2	0.6404212976968573
1103	3	0.6249778137186475
1103	4	0.7244278435529168
1103	5	0.2914055722782436
1103	6	0.666798454958446
1103	7	0.5327896902458491
1103	8	0.5238597115385559
1103	9	0.7874021380845662
1103	10	0.740081789245778
1103	11	0.8280250323300038
1103	12	0.9373917338844393
1103	13	0.7951533121395613
1103	14	1.0923486072888977
1103	15	0.9514885710495662
1103	16	1.1764276545016585
1103	17	1.0369497311731166
1103	18	1.276309665166579
1103	19	1.3366640933783884
1103	20	1.1166819458082813
1103	21	0.9273412747001899
1103	22	1.080938746660081
1103	23	1.3824399135739256
1103	24	1.2528757783841087
1103	25	1.2362710871335665
1104	1	0.36949185455381794
1104	2	0.37333972929885206
1104	3	0.3793933913684562
1104	4	0.5926956087874657
1104	5	0.3748860447799842
1104	6	0.3227295059315196
1104	7	0.534246788792359
1104	8	0.4437561597606674
1104	9	0.49246284384496875
1104	10	0.5375151691987909
1104	11	0.498047513965993
1104	12	0.5069254436482495
1104	13	0.4474098245338496
1104	14	0.5428282288814188
1104	15	0.7434400652167668
1104	16	0.4383110625734119
1104	17	0.5601803542871266
1104	18	0.8233884550855137
1104	19	0.6499768819962455
1104	20	0.7998615945176937
1104	21	0.7203900009411868
1104	22	0.5350955784458762
1104	23	0.839735625231917
1104	24	0.6123749040982673
1104	25	0.5243945584691474
1105	1	0.41888540028411825
1105	2	0.5458885636910544
1105	3	0.5015163421707278
1105	4	0.5962069018843863
1105	5	0.40684526849587854
1105	6	0.29048937876787306
1105	7	0.46307730000125247
1105	8	0.5834184175875805
1105	9	0.48537923013886086
1105	10	0.7403200290407028
1105	11	0.6253851330227107
1105	12	0.7042728518717212
1105	13	0.6624676821532987
1105	14	0.7899926607029002
1105	15	0.8689808220428219
1105	16	0.8410228598814058
1105	17	0.7361322242762398
1105	18	0.7970564102286986
1105	19	0.7632326237122484
1105	20	0.7728078329941042
1105	21	0.6382252117992024
1105	22	0.3113401505351163
1105	23	0.6930411406331345
1105	24	0.7797039403871722
1105	25	0.8375255741013916
1106	1	0.5839953035534649
1106	2	0.7099397269666561
1106	3	0.6911223753685327
1106	4	0.7246483351774273
1106	5	0.7473809218997616
1106	6	0.6397851722663788
1106	7	0.43359213081418346
1106	8	0.7441912202970471
1106	9	0.7019186789460008
1106	10	0.9054031644326272
1106	11	0.8127810552887951
1106	12	0.932003386693907
1106	13	0.8703776603727036
1106	14	1.08881515586355
1106	15	1.1576889338367522
1106	16	1.1886076224785072
1106	17	1.0839075318955054
1106	18	1.3527399350227967
1106	19	0.9307565668741802
1106	20	0.971657236915739
1106	21	0.820088988662178
1106	22	0.9275757839335341
1106	23	1.2839282985326221
1106	24	1.2311191862430775
1106	25	1.300852525409212
1107	1	0.3108365269642732
1107	2	0.32054442757315016
1107	3	0.48050025076761216
1107	4	0.4623945150986515
1107	5	0.40659099895253803
1107	6	0.2728076896991709
1107	7	0.4791865002239817
1107	8	0.2985706268956392
1107	9	0.3469773045272433
1107	10	0.1766197816433106
1107	11	0.32551852321280705
1107	12	0.4468407099459668
1107	13	0.46945172466899976
1107	14	0.6494332324657606
1107	15	0.6491772245604963
1107	16	0.65275220995224
1107	17	0.7390548262483705
1107	18	1.0921253784194533
1107	19	0.8928900029914786
1107	20	0.7908375347780421
1107	21	0.7694187808766415
1107	22	0.6525785119326599
1107	23	0.7621021935169736
1107	24	0.8223110930616713
1107	25	0.9136885533016823
1108	1	0.2910594253627039
1108	2	0.4002861278707641
1108	3	0.24946735895648686
1108	4	0.4238427056060843
1108	5	0.368291717591994
1108	6	0.4789597626840987
1108	7	0.6217116587448146
1108	8	0.537297250062089
1108	9	0.6938898213792655
1108	10	0.5849775067323577
1108	11	0.5110994212445945
1108	12	0.5412230773599234
1108	13	0.5681790846316659
1108	14	0.8468535685676386
1108	15	0.7897505748167504
1108	16	0.8114512734316222
1108	17	0.7994986933408583
1108	18	0.9340260144324759
1108	19	0.9731321984336958
1108	20	0.7709457911936539
1108	21	0.6841659325891243
1108	22	0.6795930575179838
1108	23	0.814536296305306
1108	24	0.8724901608565716
1108	25	1.067652473092643
1109	1	0.06721903593665957
1109	2	-0.06269344212637762
1109	3	0.14569571759597721
1109	4	0.19115496870242765
1109	5	-0.00892158030317347
1109	6	-0.10065141977067071
1109	7	0.04912615884781957
1109	8	0.15262125561211803
1109	9	0.34227583625326474
1109	10	0.41098722721560677
1109	11	0.011330820959031485
1109	12	0.3473527652339641
1109	13	0.2463052492699589
1109	14	0.046360005066460985
1109	15	0.429439136765005
1109	16	0.3044947832696536
1109	17	0.038772689785680825
1109	18	-1.3650373498311836
1109	19	-0.05717112657543311
1109	20	0.13836695981976277
1109	21	0.21900878733443108
1109	22	0.4172063918517992
1109	23	0.010055002386984313
1109	24	0.302608294715796
1109	25	0.4421425081831387
1110	1	0.12175512156239066
1110	2	0.0031813671833837986
1110	3	0.21042994894656886
1110	4	-0.07784303226056011
1110	5	0.10046821004043238
1110	6	0.08356738473186454
1110	7	0.17687474509323664
1110	8	0.003515990297977112
1110	9	0.39500419142652987
1110	10	0.46168472071418165
1110	11	0.36989812308201664
1110	12	-0.18768641182150958
1110	13	0.00958140011024966
1110	14	0.1724823062566756
1110	15	0.24108330548735069
1110	16	0.33515295941530354
1110	17	-0.20043581383574605
1110	18	0.35966668305275107
1110	19	0.22106669437292226
1110	20	0.18344636952338078
1110	21	0.18424209213912235
1110	22	0.1438359512613161
1110	23	0.20451489349643737
1110	24	0.372280996444033
1110	25	0.366532220982144
1111	1	0.006471512201475349
1111	2	0.2967548658691044
1111	3	0.26427763963964424
1111	4	0.16691122776801137
1111	5	0.13613176129001658
1111	6	0.3350602556137616
1111	7	0.24236645834056247
1111	8	0.09805818396735333
1111	9	0.474206893758911
1111	10	0.34617623176429646
1111	11	0.20613264073798376
1111	12	0.3341685698215002
1111	13	0.3445444083899645
1111	14	0.22104685419778036
1111	15	0.4687469944976712
1111	16	0.2564739885917007
1111	17	0.3761155498103861
1111	18	0.6310358914543741
1111	19	0.3275625945097147
1111	20	0.12448875539119797
1111	21	0.5248255162636966
1111	22	0.2923681328358967
1111	23	0.6796808359317055
1111	24	0.6525566956723523
1111	25	0.20362931711914456
1112	1	-0.10104190123463042
1112	2	0.25223132623334266
1112	3	0.24909929761591698
1112	4	0.42657759216006497
1112	5	0.39506775463997457
1112	6	0.48047966164413936
1112	7	0.10404937226786615
1112	8	0.29852746530945495
1112	9	0.5963163927387356
1112	10	0.6972213462508066
1112	11	0.6083294215133179
1112	12	0.6829318887967872
1112	13	0.41330659383544344
1112	14	0.47523486900924183
1112	15	0.6008281552322003
1112	16	0.7201292443915932
1112	17	0.6692013958014655
1112	18	0.6955405389266076
1112	19	0.6125833758895993
1112	20	0.4892189752959837
1112	21	0.590601228498258
1112	22	0.6055548629802274
1112	23	0.7424304014119443
1112	24	0.471847106010145
1112	25	0.5785091629590866
1113	1	-0.21083652552785637
1113	2	-0.11551603248265963
1113	3	-0.2075551387063257
1113	4	-0.04810879358500131
1113	5	-0.1951474994219069
1113	6	-0.07704679823562799
1113	7	-0.2828613368098729
1113	8	0.029203427037877816
1113	9	-0.057941178314862896
1113	10	-0.3709085400069867
1113	11	-0.3175021948678136
1113	12	0.0500208786644194
1113	13	-0.041789508447870105
1113	14	-0.08989942958050534
1113	15	-0.4111334038544783
1113	16	-0.3296698957211177
1113	17	-0.2537510093428068
1113	18	-0.30651641684659126
1113	19	-0.2536827940369264
1113	20	-0.45937688708441726
1113	21	-0.3495087505219985
1113	22	-0.2968672156138439
1113	23	-0.8113293946235416
1113	24	-0.1350985065889887
1113	25	-0.1931137096665836
1114	1	-0.8381633510639581
1114	2	-0.6691784730459247
1114	3	-0.4865311518425841
1114	4	-0.7085402635225861
1114	5	-0.7034313563316772
1114	6	-1.2522938215403876
1114	7	-1.169042069289219
1114	8	-0.8123131656629639
1114	9	-0.769716068681241
1114	10	-0.9863445607007293
1114	11	-1.655029679379517
1114	12	-1.2697021072667667
1114	13	-1.2150121442197275
1114	14	-1.2183169656219803
1114	15	-1.2159579359906658
1114	16	-1.8940809466306519
1114	17	-4.131407707468448
1114	18	-2.929680101744303
1114	19	-1.937286930563701
1114	20	-0.9990116205907142
1114	21	-1.888467811446212
1114	22	-2.086708688759316
1114	23	-2.023333259673336
1114	24	-1.5380062756254773
1114	25	-1.3677038615438661
1115	1	-0.9279813886057724
1115	2	-0.5138968621250619
1115	3	-0.57538013182754
1115	4	-0.45423214965687464
1115	5	-0.3703484837978408
1115	6	-0.5118925704403239
1115	7	-1.5510224144586005
1115	8	-0.8573596691388374
1115	9	-0.7754796768990602
1115	10	-0.5115204395413357
1115	11	-1.3437544179086487
1115	12	-0.9123258271084111
1115	13	-0.7700030701217031
1115	14	-0.7624760531621178
1115	15	-2.0432464033027884
1115	16	-0.9340767414690703
1115	17	-0.8660049326422694
1115	18	-0.9875800814602016
1115	19	-0.9196160074608599
1115	20	-0.6642052982721722
1115	21	-1.4597407316998172
1115	22	-1.5420345979185868
1115	23	-0.9738569323573998
1115	24	-0.9661269822517156
1115	25	-1.2356996258540178
1116	1	-0.7417605781292487
1116	2	-1.4507222671511286
1116	3	-1.04973591265556
1116	4	-0.5702142037161124
1116	5	-1.2388341005365646
1116	6	-0.7791890179706797
1116	7	-0.6779293013625178
1116	8	-1.4775685033538437
1116	9	-1.352924671403585
1116	10	-2.116589654238826
1116	11	-0.8998523966684999
1116	12	-0.8283117867377285
1116	13	-1.2229215765579013
1116	14	-1.1697893407726028
1116	15	-0.810673395281917
1116	16	-0.6661174233631542
1116	17	-0.8841667560945154
1116	18	-0.6266334450786175
1116	19	-0.8718032189637839
1116	20	-1.1792811063100217
1116	21	-1.5595875533960921
1116	22	-0.6813745750264104
1116	23	-0.521221195345575
1116	24	-1.152109153170528
1116	25	-0.9472440604781553
1201	1	0.43474444672457224
1201	2	0.40827422632371935
1201	3	0.6514838860443284
1201	4	0.6953722951239669
1201	5	0.730987819561868
1201	6	0.6782188305701595
1201	7	0.6858989084083333
1201	8	0.19036279978629267
1201	9	0.4433810748612359
1201	10	0.6016904437996402
1201	11	0.30602100564433954
1201	12	-0.20096176471950855
1201	13	0.15577712999435533
1201	14	0.7112027031633161
1201	15	0.5224388452117239
1201	16	0.9811189343526721
1201	17	1.032292931978823
1201	18	1.2454193636928323
1201	19	0.7541759910245176
1201	20	0.7678888142823155
1201	21	0.7085187481869255
1201	22	0.897492191223094
1201	23	1.1357783052832815
1201	24	0.8941195566270892
1201	25	0.921689044550412
1202	1	0.7049373179943643
1202	2	0.7911833351316382
1202	3	0.5210645183098741
1202	4	0.9465667405825187
1202	5	0.9046917474779335
1202	6	0.802274512570591
1202	7	0.7074974610337676
1202	8	0.8746643037023318
1202	9	1.1860725035333388
1202	10	1.3017027764887317
1202	11	0.9686197012127309
1202	12	1.1092694813743122
1202	13	1.0565118785537067
1202	14	1.2192152276538764
1202	15	0.8963678855704984
1202	16	1.2228529903561594
1202	17	1.307836809924794
1202	18	1.6037557257093837
1202	19	1.3773670225622325
1202	20	1.2972898360973701
1202	21	1.3509856456328961
1202	22	1.494039982754515
1202	23	1.4400558049549872
1202	24	1.545003287340009
1202	25	1.5029423036769174
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2211	9	0.2203197035402269
2211	10	0.4186049853734488
2211	11	0.14392605553180005
2211	12	0.4251956493154836
2211	13	0.2537963165558139
2211	14	0.6259600265022272
2211	15	0.4770055590120279
2211	16	0.34139654562854105
2211	17	0.48120213153512736
2211	18	0.4888747919270173
2211	19	0.2787088184793802
2211	20	0.19048811586709746
2211	21	0.2934710496230082
2211	22	0.12893509933466873
2211	23	0.675331031383898
2211	24	0.512968640014833
2211	25	0.48610607287769625
2212	1	-0.00634538220735692
2212	2	-0.04892725541899168
2212	3	-0.016784465656307646
2212	4	-0.14298309502108708
2212	5	-0.12838058959956555
2212	6	-0.11648210353892097
2212	7	-0.23973769531456668
2212	8	-0.047809671676105836
2212	9	0.07557657336715096
2212	10	-0.04284192777438989
2212	11	-0.22332057037462505
2212	12	0.10660448627664465
2212	13	-0.08964673481092689
2212	14	-0.0957730086970301
2212	15	-0.14434030509985263
2212	16	0.12401401716392968
2212	17	0.1018230315521933
2212	18	-2.5765745440867533
2212	19	-0.9696157119549369
2212	20	-0.27040462537460996
2212	21	-0.29079530675312526
2212	22	-0.09761875301313694
2212	23	-0.04318944968678551
2212	24	-0.6190938923133658
2212	25	-0.30167957750924757
2213	1	-0.018619895196387404
2213	2	0.059346388293054275
2213	3	-0.2860589266935989
2213	4	-0.24851354056944785
2213	5	-0.17596545569212907
2213	6	-0.12523542277943278
2213	7	-0.007618971688302167
2213	8	-0.3829563768016939
2213	9	-0.22432545004333093
2213	10	-0.2806361959609802
2213	11	-0.3069864680092138
2213	12	-0.41113721553061566
2213	13	-0.10693605404558326
2213	14	-0.12736716610201881
2213	15	-0.35709200578759237
2213	16	-0.7600284208214276
2213	17	-0.4201567614164716
2213	18	-0.2946350173744108
2213	19	-0.36894183936254876
2213	20	-1.0928396180172228
2213	21	-0.6849897365023025
2213	22	-0.4391057654442392
2213	23	-0.8714340735964399
2213	24	-0.5640826714694498
2213	25	-0.6412001068608859
2214	1	-1.3906792584129732
2214	2	-3.433734893339434
2214	3	-1.5542042294581009
2214	4	-1.1478287665085887
2214	5	-1.1537300595676747
2214	6	-1.1716812921561583
2214	7	-1.286362947652652
2214	8	-0.9900269311137961
2214	9	-1.1467834582299687
2214	10	-1.3841050322886872
2214	11	-1.0331310851930908
2214	12	-1.5069190799066376
2214	13	-1.397617400203309
2214	14	-2.613627525426928
2214	15	-2.8076219192834486
2214	16	-2.0366616150222185
2214	17	-2.3389555838309235
2214	18	-1.638945303100222
2214	19	-3.8027718153672296
2214	20	-2.317308595493884
2214	21	-2.2845453189433726
2214	22	-2.4354635175463457
2214	23	-2.516789668135992
2214	24	-2.7042929834086316
2214	25	-2.5199856209455334
2215	1	-0.8033999184933087
2215	2	-2.4983847684887124
2215	3	-1.1761605859883382
2215	4	-0.6577972824134832
2215	5	-0.679214806066426
2215	6	-1.40415007947567
2215	7	-1.6959004571521632
2215	8	-1.622194991042143
2215	9	-1.2897462201450693
2215	10	-2.9681067693422065
2215	11	-1.9349517344168419
2215	12	-2.2231909287829836
2215	13	-1.464784917302424
2215	14	-2.7788028592669036
2215	15	-2.5047166972662964
2215	16	-2.215220946256615
2215	17	-2.482992061954903
2215	18	-1.4173613274189734
2215	19	-1.9216991848168021
2215	20	-2.2011181710014256
2215	21	-1.9449817536410166
2215	22	-1.4728818217478477
2215	23	-2.09107150507009
2215	24	-1.8616808891734777
2215	25	-1.9993776522124946
2216	1	-1.085296768124227
2216	2	-0.9836172363816864
2216	3	-1.6223170903872983
2216	4	-2.3549958842637118
2216	5	-1.4001275689984602
2216	6	-1.2118821083224205
2216	7	-1.2509186138378467
2216	8	-1.503158513382516
2216	9	-1.5952614904559184
2216	10	-1.4337756208539432
2216	11	-1.9342835343930531
2216	12	-2.1411702964431214
2216	13	-2.2353744855642006
2216	14	-1.990625099365701
2216	15	-1.9192426123236004
2216	16	-1.243183091974636
2216	17	-1.41498918407774
2216	18	-1.390260845317897
2216	19	-1.6768292274750962
2216	20	-1.4399590323251346
2216	21	-1.8288401484880268
2216	22	-2.508265548145573
2216	23	-2.3424209348765146
2216	24	-2.166337045168021
2216	25	-2.167888690684279
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94321.0
3	4.0
4	768.0
5	4518.0
6	5560.0
7	5022.0
8	4546.0
9	4355.0
10	3886.0
11	3905.0
12	3780.0
13	3639.0
14	3699.0
15	3726.0
16	3893.0
17	4188.0
18	4560.0
19	5506.0
20	7225.0
21	9062.0
22	11617.0
23	13740.0
24	16089.0
25	18316.0
26	20443.0
27	23058.0
28	25985.0
29	31154.0
30	38189.0
31	47974.0
32	60947.0
33	78067.0
34	101696.0
35	133634.0
36	183646.0
37	290492.0
38	797229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99511798446799	18.730404253399023	12.49610632333091	25.77837143880208
2	14.60226533507255	20.46322373267131	41.48535715615761	23.449153776098534
3	18.521889602620117	24.930478107495595	29.869893707676283	26.677738582208004
4	12.224335694909335	17.08570978102585	38.789623381101684	31.90033114296313
5	12.894232815492657	38.013262009325	36.213507709570706	12.878997465611638
6	31.26628931073066	37.437345085330605	18.526416366145508	12.769949237793233
7	27.44225584211117	31.531927074669934	23.010384471066995	18.0154326121519
8	24.46203996129502	37.38202694357845	21.018486414813502	17.137446680313026
9	26.324203331017102	15.186915774870322	20.371882823013543	38.116998071099026
10	14.68711769171627	28.991600702117477	35.22622979357166	21.095051812594594
11	33.91157727378009	22.293395253386898	25.416541145421057	18.378486327411956
12	23.180656336042198	24.58750518610501	32.34417749099866	19.88766098685413
13	28.833223486495125	22.178294232533265	25.906478191090947	23.082004089880666
14	20.580879031813474	22.385057832567487	27.902678413398156	29.131384722220876
15	22.558527870103454	31.477260802345995	23.337448696986886	22.62676263056366
16	21.910305782774113	28.125670936806696	28.16387202345837	21.800151256960817
17	21.52604064161193	28.73728408267202	28.106668394601687	21.630006881114365
18	21.399511286381344	27.580674037693854	30.01495173348172	21.004862942443083
19	23.3783460311857	27.074206877875756	27.481086204353616	22.066360886584928
20	23.442222306040005	27.594055642758285	28.06819028055424	20.895531770647462
21	22.79418911724043	26.985226167638583	27.110279784497703	23.11030493062329
22	23.057175586656502	28.420194455310916	27.170117691545542	21.352512266487047
23	22.12325276187658	28.105279649976932	27.762142537299724	22.00932505084677
24	23.022211691661973	27.054889739392813	28.363027095507615	21.559871473437603
25	22.579592239375852	27.6062414590484	27.95489120290448	21.85927509867126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	230.0
1	230.0
2	343.5
3	457.0
4	457.0
5	457.0
6	873.0
7	1289.0
8	1289.0
9	1289.0
10	2087.5
11	2886.0
12	2886.0
13	2886.0
14	5583.5
15	8281.0
16	8281.0
17	8281.0
18	15739.5
19	23198.0
20	23198.0
21	23198.0
22	38178.5
23	53159.0
24	53159.0
25	53159.0
26	77329.0
27	101499.0
28	101499.0
29	101499.0
30	132316.5
31	163134.0
32	163134.0
33	163134.0
34	185130.5
35	207127.0
36	207127.0
37	207127.0
38	223244.0
39	239361.0
40	239361.0
41	239361.0
42	250250.5
43	261140.0
44	261140.0
45	261140.0
46	265049.5
47	268959.0
48	268959.0
49	268959.0
50	258252.0
51	247545.0
52	247545.0
53	247545.0
54	219265.0
55	190985.0
56	190985.0
57	190985.0
58	163316.0
59	135647.0
60	135647.0
61	135647.0
62	110234.0
63	84821.0
64	84821.0
65	84821.0
66	65373.0
67	45925.0
68	45925.0
69	45925.0
70	33250.0
71	20575.0
72	20575.0
73	20575.0
74	14226.5
75	7878.0
76	7878.0
77	7878.0
78	5424.5
79	2971.0
80	2971.0
81	2971.0
82	1960.5
83	950.0
84	950.0
85	950.0
86	622.5
87	295.0
88	295.0
89	295.0
90	189.0
91	83.0
92	83.0
93	83.0
94	54.5
95	26.0
96	26.0
97	26.0
98	22.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35799943822370395
2	0.04070702592631448
3	0.036017499186584666
4	0.036355918642029084
5	0.0424958144765207
6	0.017646157319601882
7	0.016969318408713043
8	0.023592670608125258
9	0.0204018585996493
10	0.01977336532525252
11	0.019144872050855744
12	0.019966747871220762
13	0.019725019688760462
14	0.027702049709950355
15	0.027943777892410652
16	0.02103035187404608
17	0.022238992786347576
18	0.08649034368429526
19	0.024366200791998218
20	0.02228733842283964
21	0.025429804794823537
22	0.01962832841577634
23	0.02228733842283964
24	0.018564724412951022
25	0.02228733842283964
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2068439.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.09834523145986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.22030302050888	61.56364785691319
2	10.953292306911107	16.013350849433632
3	2.64229903576847	5.794430613640713
4	0.9028516790951637	2.63987854925206
5	0.41152331323402136	1.5040836610787345
6	0.23579613247938472	1.0341784257732667
7	0.1285172158854025	0.6576077070484047
8	0.09783930602456803	0.572151309519226
9	0.0612238977008437	0.4027829049496669
>10	0.30879561961195834	3.9224327584114107
>50	0.02076640193318523	1.05641242441754
>100	0.01340884947364625	2.0095982885583616
>500	0.00238815626352389	1.2171427229292358
>1k	9.95065109801541E-4	1.612301928074571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4191	0.2026165625382233	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3825	0.18492205958212932	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3587	0.17341579809701907	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3348	0.16186119097541674	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2495	0.1206223630476896	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2454	0.11864019195151512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.834563649205996E-5
2	0.0	0.0	0.0	0.0	4.834563649205996E-5
3	0.0	0.0	0.0	0.0	4.834563649205996E-5
4	0.0	0.0	0.0	4.834563649205996E-5	4.834563649205996E-5
5	0.0	0.0	0.0	4.834563649205996E-5	4.834563649205996E-5
6	0.0	0.0	0.0	4.834563649205996E-5	4.834563649205996E-5
7	0.0	0.0	0.0	4.834563649205996E-5	4.834563649205996E-5
8	0.0	0.0	0.0	4.834563649205996E-5	4.834563649205996E-5
9	0.0	0.0	0.0	9.669127298411991E-5	4.834563649205996E-5
10	0.0	0.0	0.0	9.669127298411991E-5	4.834563649205996E-5
11	0.0	0.0	0.0	9.669127298411991E-5	4.834563649205996E-5
12	0.0	0.0	0.0	9.669127298411991E-5	1.9338254596823983E-4
13	0.0	0.0	0.0	9.669127298411991E-5	1.9338254596823983E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	50	8.7310305E-5	15.196961	5
TAGGACC	760	0.0	13.497302	4
TCCAACG	185	0.0	12.325737	18
CCAACGT	85	3.9667684E-6	12.288093	19
CCAACGA	95	1.0464883E-6	11.994121	19
GGCGAGG	930	0.0	11.843657	19
TCAATAC	225	0.0	11.397721	3
TGCACCG	60	0.005885519	11.081119	5
TGGCGAG	2125	0.0	10.954197	18
GGTATCA	945	0.0	10.687042	1
GTCCTAC	2135	0.0	10.62093	1
TCCTACA	2345	0.0	10.5311985	2
AGGACCT	1525	0.0	10.525764	5
TGTAGGA	2175	0.0	10.524588	2
GTCCTAA	1260	0.0	10.510606	1
TAAGACT	200	1.8189894E-12	10.447911	4
GATATAC	420	0.0	10.434989	1
GTAGGAC	2145	0.0	10.361565	3
CGGTTTC	130	3.8326834E-7	10.232685	13
GGACCTG	1495	0.0	10.162242	6
>>END_MODULE