FastQCFastQC Report
Thu 26 May 2016
SRR1513010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068439
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38000.18371341866982782No Hit
CTGTAGGACGTGGAATATGGCAAGA36580.17684833828795532No Hit
CTTTAGGACGTGAAATATGGCGAGG35190.17012829481555897No Hit
GTCCTAAAGTGTGTATTTCTCATTT34580.16717921098954333No Hit
GTATCAACGCAGAGTACTTTTTTTT30920.14948470803344938No Hit
GTCCTACAGTGTGCATTTCTCATTT23650.11433743030372179No Hit
TATCAACGCAGAGTACTTTTTTTTT21350.10321793391054801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8000.013.0831784
GGCGAGG9400.012.11573219
CGAGGGG658.023245E-411.69267910
TCCAACG1501.8007995E-1011.38851318
TGGCGAG21650.011.17810118
GGTATCA9450.011.0671231
AATCCCG1752.1827873E-1110.84646419
TACACCG803.7170743E-410.7044185
GTCCTAC20550.010.3635861
AGGACCT16200.010.2785925
TTAGGAC13800.010.1968733
TATGGCG24150.010.14031216
GGACCTG15100.010.0792286
GGCGAGA13000.010.074697519
AGGACGT22500.010.0647965
ATGGCGA24950.010.04343217
GACGTGA12700.010.0285527
TGGGCGA1054.1045518E-59.95269810
GTCCTAA10800.09.9478351
GGACGTG22000.09.9446496
Produced by FastQC (version 0.11.5)