##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513010_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2068439 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.479510877526483 31.0 31.0 34.0 27.0 34.0 2 30.652251287081707 31.0 31.0 34.0 27.0 34.0 3 30.738900204453696 31.0 31.0 34.0 27.0 34.0 4 34.21682486164688 37.0 35.0 37.0 30.0 37.0 5 33.88474206877747 37.0 35.0 37.0 30.0 37.0 6 33.76519539614173 37.0 35.0 37.0 29.0 37.0 7 33.64093647431711 37.0 35.0 37.0 28.0 37.0 8 33.52276910269048 37.0 35.0 37.0 28.0 37.0 9 34.99179912968185 39.0 35.0 39.0 28.0 39.0 10 34.870056114780276 39.0 35.0 39.0 27.0 39.0 11 34.90499405590399 39.0 35.0 39.0 27.0 39.0 12 34.7597299219363 39.0 35.0 39.0 27.0 39.0 13 34.74628113277694 39.0 35.0 39.0 27.0 39.0 14 35.804464139382404 40.0 36.0 41.0 27.0 41.0 15 35.81380113215812 40.0 36.0 41.0 27.0 41.0 16 35.79143305652234 40.0 36.0 41.0 27.0 41.0 17 35.76008526236452 40.0 36.0 41.0 27.0 41.0 18 35.6905468326598 40.0 36.0 41.0 27.0 41.0 19 35.67880126027405 40.0 36.0 41.0 27.0 41.0 20 35.66444840771229 40.0 36.0 41.0 27.0 41.0 21 35.5968031931326 40.0 36.0 41.0 27.0 41.0 22 35.56719197423758 40.0 36.0 41.0 27.0 41.0 23 35.45116631430755 40.0 36.0 41.0 26.0 41.0 24 35.43110819318336 40.0 36.0 41.0 26.0 41.0 25 35.32529893315684 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6474158170973858 1101 2 0.5986235743189603 1101 3 0.6556447468617321 1101 4 0.44773545996540065 1101 5 0.6797241173717268 1101 6 0.6859841675403402 1101 7 0.6812160253194648 1101 8 0.6859480802009656 1101 9 0.6583361899500133 1101 10 0.8958922094570951 1101 11 0.8033838202390058 1101 12 0.9589189765356352 1101 13 0.9619627468163188 1101 14 1.0555570834345218 1101 15 1.145926127574647 1101 16 1.2890239255928364 1101 17 1.1394832491195643 1101 18 0.588510651210278 1101 19 0.9189104920281821 1101 20 0.9752322320323259 1101 21 1.0610380904918486 1101 22 1.036990285166219 1101 23 1.0035923983820538 1101 24 0.948723057946026 1101 25 0.9814369751082594 1102 1 0.7045281915009163 1102 2 0.6166029623439897 1102 3 0.6276841595784717 1102 4 0.5453171347745354 1102 5 0.6759444594268658 1102 6 0.6410760637489474 1102 7 0.6256119280453873 1102 8 0.6556681742520567 1102 9 0.9235138846912605 1102 10 0.9141585742639506 1102 11 0.7654419407947088 1102 12 0.9303876872871015 1102 13 0.9234899278096691 1102 14 1.0752022188529082 1102 15 0.9754326547867649 1102 16 1.0732312281811005 1102 17 1.0318542282510847 1102 18 0.9854375377203013 1102 19 1.10774716024725 1102 20 1.1310986218114607 1102 21 1.0202809771791266 1102 22 1.1062324221278175 1102 23 1.0695922272550717 1102 24 0.9970863101649883 1102 25 1.0701825707021442 1103 1 0.6473619888756623 1103 2 0.646709843015639 1103 3 0.696018397533134 1103 4 0.6418855744538874 1103 5 0.6741088676984859 1103 6 0.8542440115489143 1103 7 0.7685629426876233 1103 8 0.8739971108099027 1103 9 1.0283811986246363 1103 10 0.9609967535251513 1103 11 1.0677927380571077 1103 12 1.176172715871786 1103 13 1.1106351910454464 1103 14 1.2555949012108059 1103 15 1.050330518301287 1103 16 1.153633250021052 1103 17 1.35805950645711 1103 18 1.234408457641237 1103 19 1.3432524420176222 1103 20 1.3120268580435024 1103 21 0.9862869770067704 1103 22 1.218484694622319 1103 23 1.2926764490799556 1103 24 1.3292435906521902 1103 25 1.278902217949451 1104 1 0.6524567577208984 1104 2 0.7208546258200599 1104 3 0.6457567796835448 1104 4 0.5743324660972107 1104 5 0.614522780932468 1104 6 0.819193230836369 1104 7 0.7080099986352408 1104 8 0.6100699176481683 1104 9 0.8998540997708915 1104 10 0.957329962628414 1104 11 1.021027194937922 1104 12 0.8513610312986231 1104 13 0.9034947669800815 1104 14 1.0090177069136388 1104 15 1.0684050590173584 1104 16 1.0331803044992824 1104 17 1.0135612289805565 1104 18 1.0097481200293075 1104 19 1.087022437382366 1104 20 1.0115444980461206 1104 21 0.8504936210448619 1104 22 1.0909033473004115 1104 23 1.0710959486598455 1104 24 1.090314160013314 1104 25 1.2341622929416687 1105 1 0.5764834308122602 1105 2 0.6366344477541617 1105 3 0.5843608912043372 1105 4 0.45075228443688076 1105 5 0.48629460242504763 1105 6 0.507517323366109 1105 7 0.6580534077700477 1105 8 0.7226688618191375 1105 9 0.7534478596104748 1105 10 0.7187630690455364 1105 11 0.6844590496719221 1105 12 0.6646170206624973 1105 13 0.7908727384892558 1105 14 0.9068599509713238 1105 15 0.8852858172727025 1105 16 0.9983904655623803 1105 17 0.8245317822329881 1105 18 1.0474961128432767 1105 19 0.9601176714343751 1105 20 0.900938588601754 1105 21 0.9113973093708125 1105 22 0.9619852041217314 1105 23 0.958342224566465 1105 24 0.9855683068769991 1105 25 1.1088108098493024 1106 1 0.6776057797357673 1106 2 0.6401956320777664 1106 3 0.7136226190567356 1106 4 0.597996038364009 1106 5 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1107 18 0.5984386546224201 1107 19 0.9191400993120453 1107 20 0.8116316537215411 1107 21 0.8031401570487304 1107 22 0.828492989034018 1107 23 0.8824849679286757 1107 24 0.8313664277845447 1107 25 0.7504452890108269 1108 1 0.6669185777640081 1108 2 0.5338502845179711 1108 3 0.6052785822163322 1108 4 0.6155903773746729 1108 5 0.6761494937603061 1108 6 0.5920189673973582 1108 7 0.6010957818592999 1108 8 0.7298416195578099 1108 9 0.653770697777837 1108 10 0.7616234692721804 1108 11 0.8692092994537362 1108 12 0.9382446600470686 1108 13 0.780551553831863 1108 14 0.9540167563760207 1108 15 0.808673627498905 1108 16 0.8022266964583764 1108 17 0.822285852289852 1108 18 0.8462169947433438 1108 19 0.9030061918624241 1108 20 0.9437311457707196 1108 21 1.0889913014072832 1108 22 0.9556252465864006 1108 23 0.8505112575194076 1108 24 0.894834492492798 1108 25 0.903907310235823 1109 1 0.24240926605569868 1109 2 0.20428784009829215 1109 3 0.24344027186340966 1109 4 0.3850394408415383 1109 5 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6 -2.680884023933455 2214 7 -1.2848390029616183 2214 8 -1.7983237007757786 2214 9 -1.1824845417757643 2214 10 -1.4665912785266002 2214 11 -1.2593488238532515 2214 12 -1.3672730926508478 2214 13 -1.3866252171978317 2214 14 -1.641752252603304 2214 15 -2.084682836595917 2214 16 -1.527121975541938 2214 17 -1.6944593006687683 2214 18 -1.6254685302780985 2214 19 -3.2825011907594615 2214 20 -1.9350171763606738 2214 21 -2.332724025468174 2214 22 -2.1405939399925913 2214 23 -2.0457253740472936 2214 24 -1.418696324562589 2214 25 -1.2597147537969775 2215 1 -1.474396294219595 2215 2 -1.2847971012225727 2215 3 -1.2738303437927527 2215 4 -1.0926307046619215 2215 5 -1.9115448212115176 2215 6 -1.75386089940843 2215 7 -1.8344785643556065 2215 8 -2.6963901430730317 2215 9 -2.113167420941977 2215 10 -1.5031985958645677 2215 11 -2.1336317464458077 2215 12 -2.840889420953964 2215 13 -2.4953973374887894 2215 14 -2.512123547793834 2215 15 -3.7107560099755688 2215 16 -3.0875594958980344 2215 17 -2.755841343877975 2215 18 -3.3484269495593892 2215 19 -4.0284820831707115 2215 20 -3.456412247471299 2215 21 -2.3926328301582203 2215 22 -3.5088210597407183 2215 23 -3.4803405081261154 2215 24 -2.4641343877479116 2215 25 -2.656959352646176 2216 1 -2.03259456182818 2216 2 -1.7755301101337935 2216 3 -2.1184473538596578 2216 4 -1.6492387751812458 2216 5 -1.7193646593025989 2216 6 -2.201639608782493 2216 7 -3.498397179348892 2216 8 -2.661944842567138 2216 9 -2.707228683468955 2216 10 -3.013248222250393 2216 11 -3.3392192469415 2216 12 -2.8588438621189347 2216 13 -3.182635538475651 2216 14 -3.2825445789576193 2216 15 -3.4678818717808184 2216 16 -3.3769818810237098 2216 17 -3.614848872812537 2216 18 -3.3966135470361536 2216 19 -3.632856966327765 2216 20 -3.9041722619739865 2216 21 -3.731160127502978 2216 22 -4.046392086089156 2216 23 -3.007414080942638 2216 24 -3.572998306079647 2216 25 -3.268742786724097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51171.0 3 0.0 4 798.0 5 7293.0 6 8303.0 7 6687.0 8 5345.0 9 4611.0 10 4244.0 11 3737.0 12 3508.0 13 3223.0 14 3218.0 15 3167.0 16 3276.0 17 3478.0 18 4008.0 19 4596.0 20 5695.0 21 7493.0 22 9771.0 23 11831.0 24 14108.0 25 16269.0 26 18556.0 27 20620.0 28 23242.0 29 28267.0 30 35550.0 31 45045.0 32 57635.0 33 73778.0 34 96678.0 35 126600.0 36 174344.0 37 273277.0 38 909017.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.16077352129467 19.696943305812635 13.583167860778552 27.55911531211414 2 13.48114542526555 20.954179411797412 40.29549848508802 25.269176677849025 3 16.966466016160012 25.09153037628859 30.337515392042018 27.604488215509377 4 11.453723615954397 17.70861693850046 38.00032787376763 32.83733157177752 5 12.522327346475908 38.24691384305158 36.32995512275928 12.900803687713235 6 31.325142646743622 38.02142325960812 18.07134538631588 12.582088707332378 7 27.25515856549659 31.658256461446054 22.91928427654341 18.167300696513948 8 24.501386046646637 37.599212396530284 20.7231148072355 17.17628674958757 9 25.954112443136335 14.964412596223841 20.206922070478296 38.87455289016153 10 14.34389495040228 29.335202766011815 35.14076886145936 21.180133422126545 11 33.909488895818676 22.183173666156602 25.367717980143738 18.53961945788098 12 22.94376983025639 24.766581986656856 32.29900907948929 19.990639103597466 13 28.737249350440884 22.126202542539232 25.953113774134252 23.18343433288563 14 20.475587038094442 22.358866454544707 27.802545753604274 29.363000753756573 15 22.459425850645356 31.56298469476559 23.257516274140457 22.720073180448598 16 21.79062287243919 28.294092730704957 28.000392631676675 21.914891765179178 17 21.360340254320278 28.943185362680836 28.040950448892943 21.65552393410595 18 21.29723233058679 27.790404168594467 29.93262003491556 20.979743465903187 19 23.26872256919163 27.00559760122144 27.564149051482655 22.16153077810428 20 23.391333062262884 27.595471350738247 27.998886064835478 21.014309522163394 21 22.649093324618214 26.931556016236424 27.2804669808493 23.138883678296065 22 22.98387233234047 28.29001471161986 27.26895190707168 21.457161048967986 23 21.887616700323285 28.10481720756571 27.811649267877854 22.19591682423315 24 22.92032784143018 27.14133701791544 28.370138060634133 21.568197080020248 25 22.402749978243843 27.6167822160338 27.97198773919686 22.008480066525493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 114.0 1 114.0 2 225.5 3 337.0 4 337.0 5 337.0 6 862.0 7 1387.0 8 1387.0 9 1387.0 10 2233.0 11 3079.0 12 3079.0 13 3079.0 14 5764.5 15 8450.0 16 8450.0 17 8450.0 18 16097.0 19 23744.0 20 23744.0 21 23744.0 22 38810.5 23 53877.0 24 53877.0 25 53877.0 26 78517.0 27 103157.0 28 103157.0 29 103157.0 30 133625.0 31 164093.0 32 164093.0 33 164093.0 34 185667.5 35 207242.0 36 207242.0 37 207242.0 38 223055.5 39 238869.0 40 238869.0 41 238869.0 42 249962.5 43 261056.0 44 261056.0 45 261056.0 46 265555.0 47 270054.0 48 270054.0 49 270054.0 50 258660.5 51 247267.0 52 247267.0 53 247267.0 54 219403.5 55 191540.0 56 191540.0 57 191540.0 58 163110.5 59 134681.0 60 134681.0 61 134681.0 62 109103.5 63 83526.0 64 83526.0 65 83526.0 66 64077.0 67 44628.0 68 44628.0 69 44628.0 70 32279.0 71 19930.0 72 19930.0 73 19930.0 74 13766.5 75 7603.0 76 7603.0 77 7603.0 78 5160.0 79 2717.0 80 2717.0 81 2717.0 82 1757.0 83 797.0 84 797.0 85 797.0 86 510.0 87 223.0 88 223.0 89 223.0 90 141.5 91 60.0 92 60.0 93 60.0 94 33.5 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07885173311854979 2 9.669127298411991E-5 3 0.0 4 0.027557012800474172 5 0.07092304873385195 6 0.07831993111713713 7 0.034470438818838746 8 0.06811900181731247 9 0.048055562673107594 10 0.10708558482991279 11 0.0035775771004124364 12 0.012618211124427648 13 0.005463056923602775 14 0.006381624016951914 15 0.006139895834491614 16 0.016582553316776563 17 0.027750395346442414 18 9.66912729841199E-4 19 0.0025623187340791777 20 0.0046411811032377554 21 1.4503690947617986E-4 22 2.900738189523597E-4 23 0.0 24 0.0 25 0.0028523925530315373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2068439.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.93388117939563 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39775997988457 59.99124557020773 2 11.412556050255366 16.418989017445245 3 2.815876235726774 6.076707196699608 4 0.9985427187342648 2.873162131279251 5 0.44838661957021425 1.6127094907297332 6 0.23470501735763094 1.0129945698487088 7 0.14184507156852186 0.7142426566865044 8 0.1084376361558246 0.6240272027686098 9 0.07261179437151742 0.4700923369689098 >10 0.3307897850030996 4.141241822456301 >50 0.0206440633307517 1.0396023756630046 >100 0.014547261371917504 2.1336954528369456 >500 0.0022209448998732665 1.183055792520705 >1k 0.0010768217696354879 1.7082343838888618 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3800 0.18371341866982782 No Hit CTGTAGGACGTGGAATATGGCAAGA 3658 0.17684833828795532 No Hit CTTTAGGACGTGAAATATGGCGAGG 3519 0.17012829481555897 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3458 0.16717921098954333 No Hit GTATCAACGCAGAGTACTTTTTTTT 3092 0.14948470803344938 No Hit GTCCTACAGTGTGCATTTCTCATTT 2365 0.11433743030372179 No Hit TATCAACGCAGAGTACTTTTTTTTT 2135 0.10321793391054801 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.834563649205996E-5 2 0.0 0.0 0.0 0.0 9.669127298411991E-5 3 0.0 0.0 0.0 0.0 9.669127298411991E-5 4 0.0 0.0 0.0 0.0 9.669127298411991E-5 5 0.0 0.0 0.0 0.0 9.669127298411991E-5 6 0.0 0.0 0.0 4.834563649205996E-5 1.4503690947617986E-4 7 0.0 0.0 0.0 4.834563649205996E-5 1.4503690947617986E-4 8 0.0 0.0 0.0 4.834563649205996E-5 1.4503690947617986E-4 9 0.0 0.0 0.0 9.669127298411991E-5 1.4503690947617986E-4 10 0.0 0.0 0.0 9.669127298411991E-5 1.9338254596823983E-4 11 0.0 0.0 0.0 9.669127298411991E-5 1.9338254596823983E-4 12 0.0 9.669127298411991E-5 0.0 9.669127298411991E-5 2.4172818246029977E-4 13 0.0 1.4503690947617986E-4 0.0 9.669127298411991E-5 2.4172818246029977E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 800 0.0 13.083178 4 GGCGAGG 940 0.0 12.115732 19 CGAGGGG 65 8.023245E-4 11.692679 10 TCCAACG 150 1.8007995E-10 11.388513 18 TGGCGAG 2165 0.0 11.178101 18 GGTATCA 945 0.0 11.067123 1 AATCCCG 175 2.1827873E-11 10.846464 19 TACACCG 80 3.7170743E-4 10.704418 5 GTCCTAC 2055 0.0 10.363586 1 AGGACCT 1620 0.0 10.278592 5 TTAGGAC 1380 0.0 10.196873 3 TATGGCG 2415 0.0 10.140312 16 GGACCTG 1510 0.0 10.079228 6 GGCGAGA 1300 0.0 10.0746975 19 AGGACGT 2250 0.0 10.064796 5 ATGGCGA 2495 0.0 10.043432 17 GACGTGA 1270 0.0 10.028552 7 TGGGCGA 105 4.1045518E-5 9.952698 10 GTCCTAA 1080 0.0 9.947835 1 GGACGTG 2200 0.0 9.944649 6 >>END_MODULE