FastQCFastQC Report
Thu 26 May 2016
SRR1513009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209480
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46150.20887267592374675No Hit
GTCCTAAAGTGTGTATTTCTCATTT43420.19651682748882093No Hit
CTGTAGGACGTGGAATATGGCAAGA39560.17904665351123342No Hit
CTTTAGGACGTGAAATATGGCGAGG37850.17130727592012598No Hit
GTCCTACAGTGTGCATTTCTCATTT31980.14473993880913155No Hit
GTATCAACGCAGAGTACTTTTTTTT31040.14048554410992634No Hit
CTGTAGGACCTGGAATATGGCGAGA24270.10984485037203323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1750.014.11830718
TAGGACC8850.012.7709974
TATTGCG604.0834103E-412.67027616
GGCGAGG10550.012.24050719
GGACCGA550.00308136312.0844986
CTTATAC2500.011.397313
ATTGCGA600.005864533611.08649217
CCAACGA1701.4006218E-1010.61249819
AATGTCC6450.010.5995098
AGGACGT24450.010.5660125
TGTACCG909.541117E-510.5530645
TTAGGAC15600.010.532773
TGGCGAG25550.010.45111818
GGACGTG24400.010.4288946
CGGTTTC1553.6452548E-910.4225613
GTCCTAC25350.010.4046091
AGGACCT17350.010.4010035
GGACCTG17000.010.2210496
TGTAGGA27100.010.1636522
GACGTGA14350.010.1235497
Produced by FastQC (version 0.11.5)