##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513009_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2209480
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.293005141481252	31.0	30.0	34.0	25.0	34.0
2	29.313678784148305	31.0	30.0	34.0	23.0	34.0
3	29.539293860998967	31.0	30.0	34.0	25.0	34.0
4	32.98486295417926	35.0	35.0	37.0	26.0	37.0
5	33.1301111573764	37.0	35.0	37.0	27.0	37.0
6	33.12453835291562	37.0	35.0	37.0	26.0	37.0
7	33.012049441497545	37.0	35.0	37.0	26.0	37.0
8	32.99743378532505	37.0	35.0	37.0	26.0	37.0
9	34.338696887955535	39.0	35.0	39.0	26.0	39.0
10	34.214492097688144	39.0	35.0	39.0	25.0	39.0
11	34.270006517370604	39.0	35.0	39.0	26.0	39.0
12	34.16867588753915	39.0	35.0	39.0	25.0	39.0
13	34.16594900157503	39.0	35.0	39.0	25.0	39.0
14	35.17844877527744	39.0	35.0	41.0	25.0	41.0
15	35.12915165559317	39.0	35.0	41.0	25.0	41.0
16	35.024135090609555	39.0	35.0	41.0	24.0	41.0
17	34.972841573582926	39.0	35.0	41.0	24.0	41.0
18	34.66581367561598	39.0	34.0	41.0	19.0	41.0
19	34.90167595995438	39.0	34.0	41.0	22.0	41.0
20	34.98959709977008	39.0	35.0	41.0	24.0	41.0
21	34.92803962923403	39.0	35.0	41.0	23.0	41.0
22	34.84739395694915	39.0	35.0	41.0	22.0	41.0
23	34.57809846660753	39.0	34.0	41.0	18.0	41.0
24	34.580748411390914	39.0	34.0	41.0	18.0	41.0
25	34.4979316400239	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7022483710276219
1101	2	0.9014854937334
1101	3	0.9313493899922669
1101	4	0.9907662746131365
1101	5	0.7996507768678285
1101	6	0.9646096481034618
1101	7	1.0349738802850723
1101	8	0.7997162273018361
1101	9	0.6905737948924155
1101	10	0.7164995794357267
1101	11	0.8935101203061038
1101	12	1.1739989836564604
1101	13	0.9382855501049931
1101	14	0.8921848450929417
1101	15	0.6598533046512571
1101	16	1.1732658969504328
1101	17	1.2225514958865489
1101	18	1.3486079412142473
1101	19	0.9590685454917889
1101	20	1.1372227644740036
1101	21	1.2002830960638633
1101	22	1.165636782785434
1101	23	1.4614177085386117
1101	24	1.107423072648757
1101	25	1.3035627832705856
1102	1	0.6853336832582286
1102	2	0.713102122052149
1102	3	0.8171631238528931
1102	4	0.7947786968578257
1102	5	0.7155840783651541
1102	6	0.7405247265874877
1102	7	0.8331734748238873
1102	8	0.8018772168917394
1102	9	0.8653036884209513
1102	10	1.0279487586249019
1102	11	0.9941126787634218
1102	12	0.9251845648393129
1102	13	0.8801055050797828
1102	14	0.9583211355916816
1102	15	1.086767961411553
1102	16	1.0192828127579716
1102	17	0.963429566734753
1102	18	1.179535916037615
1102	19	1.2822279660213312
1102	20	1.1981722665023398
1102	21	1.0073886758409714
1102	22	1.246909475415677
1102	23	1.3920108195573846
1102	24	1.2323806935480661
1102	25	1.1791528174977373
1103	1	0.33054582991327663
1103	2	0.5316837740129401
1103	3	0.4817150607011058
1103	4	0.6155869939613012
1103	5	0.1923083755511854
1103	6	0.5454097549849521
1103	7	0.47731437202236293
1103	8	0.46323872805398736
1103	9	0.7414060417804151
1103	10	0.6040246684234987
1103	11	0.6904035163212683
1103	12	0.8548392397873741
1103	13	0.8469391386716936
1103	14	0.9199848516871683
1103	15	0.9845333127291909
1103	16	1.0404563699353773
1103	17	0.919605488899407
1103	18	1.347977539611847
1103	19	1.2087656230110966
1103	20	1.1397022983168341
1103	21	0.9664988936429566
1103	22	1.1473860467731072
1103	23	1.3891688079205409
1103	24	1.3401858561835098
1103	25	1.2260587614766507
1104	1	0.5529655703241154
1104	2	0.5218632292469785
1104	3	0.5205788772529125
1104	4	0.7359926296746124
1104	5	0.5025735205421924
1104	6	0.4788177356729193
1104	7	0.667372255835204
1104	8	0.6419420153747097
1104	9	0.6810313216661115
1104	10	0.7147009044275379
1104	11	0.6727145059354243
1104	12	0.6753091126601305
1104	13	0.6317528643130146
1104	14	0.8239896716522281
1104	15	0.9427503411724842
1104	16	0.746294579417544
1104	17	0.820673896615375
1104	18	1.0093935919988084
1104	19	0.9922731595679721
1104	20	1.0469570092094074
1104	21	0.9825550001271921
1104	22	0.8270011197528149
1104	23	1.15188287314421
1104	24	0.9472745959489615
1104	25	0.7855997723959618
1105	1	0.7066435662069779
1105	2	0.7836777311846603
1105	3	0.8226067861719137
1105	4	0.9510925449372039
1105	5	0.7010881256226753
1105	6	0.49308794013447965
1105	7	0.8464485488557614
1105	8	0.8585036426231412
1105	9	0.7116947010888097
1105	10	0.8871639526190123
1105	11	0.9501736800871114
1105	12	0.9637485260754204
1105	13	1.0776188070339572
1105	14	1.0720697212874342
1105	15	1.3198552113797817
1105	16	1.2078136878837356
1105	17	1.0544452322833564
1105	18	1.0081231081577968
1105	19	1.1779875183195543
1105	20	1.151165463225098
1105	21	1.2204882485581976
1105	22	0.9762257015824645
1105	23	1.1808049962037686
1105	24	1.291800424365121
1105	25	1.2187783585380174
1106	1	0.781935704877057
1106	2	0.7673137286785092
1106	3	0.7895880365539121
1106	4	0.7921684753016791
1106	5	0.8436852054656754
1106	6	0.8491787352430578
1106	7	0.6714541433761312
1106	8	0.7308319395488709
1106	9	1.0006619660221574
1106	10	1.0567542584049008
1106	11	0.9637169242076311
1106	12	0.982829066957791
1106	13	1.0002196222389728
1106	14	1.1238339493276328
1106	15	1.163532745904483
1106	16	1.1731851287277948
1106	17	1.1580372430891188
1106	18	1.2793432639180082
1106	19	0.8969733602260845
1106	20	0.9024948162306856
1106	21	0.7976053331641424
1106	22	0.8533298560891183
1106	23	1.265960049035769
1106	24	1.1302321372477593
1106	25	1.2708148248271058
1107	1	0.36430273476439723
1107	2	0.4932829447127425
1107	3	0.6390900161484154
1107	4	0.5847808481052539
1107	5	0.543839878383622
1107	6	0.4251739553800178
1107	7	0.6053775735232492
1107	8	0.5793973852456773
1107	9	0.7325442993883158
1107	10	0.5621381214430983
1107	11	0.602337860342459
1107	12	0.87945680471028
1107	13	0.8222391594303105
1107	14	0.911815226373669
1107	15	1.0533912102077352
1107	16	0.8793577560626176
1107	17	1.010840424214777
1107	18	1.3776094854253316
1107	19	1.2407704484102453
1107	20	1.178052536649247
1107	21	1.0751986470201516
1107	22	1.0053297099769551
1107	23	1.158240949987814
1107	24	1.104498856952496
1107	25	1.1803449261851782
1108	1	0.4981625886654584
1108	2	0.5515813172758719
1108	3	0.41727444773123423
1108	4	0.5638059000125111
1108	5	0.5712536696185779
1108	6	0.5620566064019386
1108	7	0.6878187192632836
1108	8	0.6149213473713857
1108	9	0.7255594498699693
1108	10	0.6300289781790553
1108	11	0.5796619414229696
1108	12	0.6804183560441217
1108	13	0.6810401734148357
1108	14	0.9257344046802913
1108	15	0.9078360463516404
1108	16	0.7660545800458394
1108	17	0.8123139561333588
1108	18	1.0836792403695057
1108	19	1.047726117027402
1108	20	0.9432422236629705
1108	21	0.8758309370658779
1108	22	0.7784416690146827
1108	23	0.9611925718466807
1108	24	1.0136002700667177
1108	25	1.0208223741775129
1109	1	0.13085194916344633
1109	2	0.05831196899577762
1109	3	0.20418045849441313
1109	4	0.3160187222861026
1109	5	0.1289768042501649
1109	6	0.10149396630741592
1109	7	0.2427615265613312
1109	8	0.20601529400837393
1109	9	0.44922520253000897
1109	10	0.5264710581619383
1109	11	0.15249181819040558
1109	12	0.3118893289975162
1109	13	0.409068359276759
1109	14	0.19184631249101614
1109	15	0.4580927103297654
1109	16	0.4096346762712457
1109	17	0.2673181282441277
1109	18	-1.0879290741131271
1109	19	0.15392514554070402
1109	20	0.3679570254923661
1109	21	0.36677875656602055
1109	22	0.48717642408631434
1109	23	0.08963818745350949
1109	24	0.5322114961586806
1109	25	0.5174490912199587
1110	1	0.34599414734825373
1110	2	0.29867193781355894
1110	3	0.4317372993964348
1110	4	0.3012722797651932
1110	5	0.4987287602072996
1110	6	0.48365200198924185
1110	7	0.6378147019370743
1110	8	0.5133183148867246
1110	9	0.7236616808781591
1110	10	0.9064189531602622
1110	11	0.7208141583108585
1110	12	0.21004561976766212
1110	13	0.5675831647716123
1110	14	0.6601037839039492
1110	15	0.6741860469482717
1110	16	0.8290515849501645
1110	17	0.5756191416662091
1110	18	0.9834162447169703
1110	19	0.7799648018900385
1110	20	0.7295811666686447
1110	21	0.8977119207937179
1110	22	0.8579553736656322
1110	23	0.7042963342118895
1110	24	0.9782295026778627
1110	25	0.9419614651480757
1111	1	0.16101358711812352
1111	2	0.37335381364772857
1111	3	0.3264999186670927
1111	4	0.182803752296671
1111	5	0.19270649358816172
1111	6	0.3562524546167225
1111	7	0.16383531058010448
1111	8	0.08237865692719737
1111	9	0.35113666616362593
1111	10	0.3275513508424126
1111	11	0.11870703647448266
1111	12	0.36061322788842887
1111	13	0.2824217193480436
1111	14	0.279386377641778
1111	15	0.44999882828609117
1111	16	0.41495165873009654
1111	17	0.3851385772911442
1111	18	0.5648939988225337
1111	19	0.48913059121101554
1111	20	0.40145409937968424
1111	21	0.6087169030119242
1111	22	0.260779901764181
1111	23	0.6695953916524786
1111	24	0.7279737598631186
1111	25	0.1843741519812525
1112	1	-0.17146274503580017
1112	2	0.14229684515232677
1112	3	0.21226425978577268
1112	4	0.3638550302728092
1112	5	0.21179060956431073
1112	6	0.23702277583138454
1112	7	-0.08518719963252153
1112	8	0.25807175543850747
1112	9	0.47232300810928507
1112	10	0.5765615708803082
1112	11	0.48885763657172276
1112	12	0.4587696343356171
1112	13	0.28054300620004824
1112	14	0.3659356400292637
1112	15	0.3747375021324686
1112	16	0.49397310491664115
1112	17	0.5043428339342384
1112	18	0.4236872989600542
1112	19	0.4072121993180815
1112	20	0.3632890662399859
1112	21	0.4406514573264104
1112	22	0.36986951296366755
1112	23	0.6325049911880782
1112	24	0.28443297062513295
1112	25	0.5118924929625592
1113	1	-0.36678518728718146
1113	2	-0.2794697807146669
1113	3	-0.4306185295190623
1113	4	-0.1610905044624289
1113	5	-0.335248424751434
1113	6	-0.3102275659726459
1113	7	-0.287495436239098
1113	8	-0.12964545683345818
1113	9	-0.31413332342847156
1113	10	-0.5877812061649124
1113	11	-0.3634982160790372
1113	12	-0.16643313215818978
1113	13	-0.3654459949700666
1113	14	-0.37016807983663114
1113	15	-0.5808379933601913
1113	16	-0.6357728136377503
1113	17	-0.6261502573867332
1113	18	-0.6304512727622935
1113	19	-0.5411004122486531
1113	20	-0.8196757067568043
1113	21	-0.6437816111703896
1113	22	-0.5227632880029134
1113	23	-0.9566644693692865
1113	24	-0.3232153421014061
1113	25	-0.33233865656988826
1114	1	-1.5555981492553776
1114	2	-1.2071827904399868
1114	3	-1.0369173422837434
1114	4	-1.2770020516393004
1114	5	-1.3272471707626927
1114	6	-1.7401261905058263
1114	7	-1.9539353460458067
1114	8	-1.3566146724253727
1114	9	-1.447669139649527
1114	10	-1.5413561520958012
1114	11	-2.140009268769461
1114	12	-1.714777170263332
1114	13	-1.5235260769355108
1114	14	-1.9150580715430223
1114	15	-1.9313414993092692
1114	16	-2.5658555324425123
1114	17	-4.621933445529912
1114	18	-3.556541938712396
1114	19	-2.505933703085887
1114	20	-1.7207601855910397
1114	21	-2.7527998829950917
1114	22	-2.8992805673501323
1114	23	-2.730044462671465
1114	24	-2.3468232784916125
1114	25	-2.1465700136639114
1115	1	-1.2351417671044906
1115	2	-0.7383180969296141
1115	3	-0.7658710389646011
1115	4	-0.8552284379829516
1115	5	-0.6178060765830153
1115	6	-0.6826021848749235
1115	7	-1.8182628564709802
1115	8	-1.2386562206574077
1115	9	-0.911138909963789
1115	10	-0.7878527925280494
1115	11	-1.6105595492223799
1115	12	-1.1521954187826964
1115	13	-0.9479934262367706
1115	14	-1.00805066717475
1115	15	-2.228078292411375
1115	16	-1.0476575918239277
1115	17	-1.0334898104215
1115	18	-1.0512740833667422
1115	19	-1.3096605127749115
1115	20	-1.0746966122649297
1115	21	-1.7566879793456707
1115	22	-1.7269477823457677
1115	23	-1.2082761167419847
1115	24	-1.0338203444105787
1115	25	-1.3549803036330061
1116	1	-1.3977319265240489
1116	2	-2.1210667607355838
1116	3	-1.5935295070007207
1116	4	-1.3373872862003786
1116	5	-1.8715720953021346
1116	6	-1.6642747944582297
1116	7	-1.3249284739683844
1116	8	-2.056471029168339
1116	9	-2.141514925954496
1116	10	-3.109823166611058
1116	11	-1.788184104572757
1116	12	-1.7172005206533214
1116	13	-2.346715178851209
1116	14	-2.0714985224646583
1116	15	-1.6215726162061017
1116	16	-1.6001432249049188
1116	17	-1.9533942904027484
1116	18	-1.6780390184194616
1116	19	-2.0919160955385365
1116	20	-2.4232501699323166
1116	21	-2.9085395777750023
1116	22	-2.043980247006864
1116	23	-2.086532996141976
1116	24	-2.6485865179391794
1116	25	-2.3017282353012902
1201	1	0.10715837817168605
1201	2	0.15057570888021843
1201	3	0.2955993889437245
1201	4	0.3014029282825419
1201	5	0.3171914847594408
1201	6	0.3076944793371439
1201	7	0.23358222052713273
1201	8	-0.23192387397332936
1201	9	0.06950040954207992
1201	10	0.34903457626913337
1201	11	-0.12202280460850545
1201	12	-0.6823532229650127
1201	13	-0.2540703348014546
1201	14	0.23641992678833645
1201	15	-0.04558681032485623
1201	16	0.4699840307136185
1201	17	0.5575929622234597
1201	18	0.7970894078866877
1201	19	0.3187362415935908
1201	20	0.4214658710100565
1201	21	0.3980047193744909
1201	22	0.5515391719231033
1201	23	0.8329267630474106
1201	24	0.5231473914110865
1201	25	0.40774083826730845
1202	1	0.5006370001409941
1202	2	0.48488381849517026
1202	3	0.2637013755802613
1202	4	0.6168340969061461
1202	5	0.6386239282753792
1202	6	0.5221865473994995
1202	7	0.48664088474740197
1202	8	0.5124497770526304
1202	9	0.8048032202266739
1202	10	0.9285371103594358
1202	11	0.622981930002986
1202	12	0.737087629682442
1202	13	0.6802269838695381
1202	14	0.8622895012637457
1202	15	0.48571439257695914
1202	16	0.6835769382808721
1202	17	0.8662582429211909
1202	18	1.1403148922957271
1202	19	0.8662390457968314
1202	20	0.7544195135559661
1202	21	0.8220921253418467
1202	22	0.8686148566517602
1202	23	1.0049669012868705
1202	24	1.1160879592692439
1202	25	0.8973429323891082
1203	1	0.4027818798532614
1203	2	0.5901208348618354
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2211	14	0.2784791222400713
2211	15	0.26005243052012617
2211	16	0.07795334997066306
2211	17	0.15249834554310127
2211	18	0.20510508894459178
2211	19	-0.01016534608643127
2211	20	-0.13979418787518938
2211	21	0.020216661551266668
2211	22	-0.18261789332410672
2211	23	0.3518653385717698
2211	24	0.2136004163069316
2211	25	0.338657521810255
2212	1	-0.06432335086134344
2212	2	0.0015445406622909275
2212	3	-0.059007508433595035
2212	4	-0.22078310338114449
2212	5	-0.08165080532146618
2212	6	-0.16147332625138944
2212	7	-0.24107286072704426
2212	8	-0.13885483701906054
2212	9	0.0032662006521704257
2212	10	0.01502005127436945
2212	11	-0.15822538971174538
2212	12	0.008065035698272993
2212	13	-0.07464807577903088
2212	14	-0.12225716142906151
2212	15	-0.23339253186926356
2212	16	0.031257361985254306
2212	17	0.08429344738754452
2212	18	-2.689831944986217
2212	19	-0.9556777540103383
2212	20	-0.39914645328838816
2212	21	-0.5165945962747998
2212	22	-0.20075712732903384
2212	23	-0.34034598296802443
2212	24	-0.6670159815268022
2212	25	-0.3089655659651598
2213	1	-0.058691778425366437
2213	2	0.12819388702708778
2213	3	-0.2794912397214837
2213	4	-0.32338486015461854
2213	5	-0.23537219589427139
2213	6	-0.21585041396634352
2213	7	0.021530133210852398
2213	8	-0.3531523338230471
2213	9	-0.18874707824592463
2213	10	-0.3107785383581927
2213	11	-0.36166759351396394
2213	12	-0.5301414882820978
2213	13	-0.24124381168567055
2213	14	-0.20251572879266888
2213	15	-0.4130700443440105
2213	16	-0.8369029745234684
2213	17	-0.4406325289413715
2213	18	-0.3118340894045488
2213	19	-0.25585508091041476
2213	20	-0.9307683758418932
2213	21	-0.6017226427921827
2213	22	-0.50657839830928
2213	23	-0.9453160848278941
2213	24	-0.6988836874115805
2213	25	-0.7549790098185127
2214	1	-1.4358903035939719
2214	2	-3.521307322176071
2214	3	-1.7636713383078835
2214	4	-1.2865354841386925
2214	5	-1.2537245960193815
2214	6	-1.2427663512199345
2214	7	-1.44968757181406
2214	8	-1.1223949629699135
2214	9	-1.3834958327005964
2214	10	-1.3777783489383921
2214	11	-1.008902196547325
2214	12	-1.49600927325978
2214	13	-1.5383211081805186
2214	14	-2.6457992990475887
2214	15	-2.90733018380984
2214	16	-2.0861930026721325
2214	17	-2.451331311809014
2214	18	-1.889802451892379
2214	19	-3.9303314232580746
2214	20	-2.257630746937309
2214	21	-2.1668094014669492
2214	22	-2.346654518639312
2214	23	-2.306264241068398
2214	24	-2.7131973930355855
2214	25	-2.4480952298802734
2215	1	-0.9883222501346616
2215	2	-2.775836205453743
2215	3	-1.433656651803247
2215	4	-0.9036296029683726
2215	5	-0.8850289151291477
2215	6	-1.4997689325173766
2215	7	-1.8982851684820723
2215	8	-1.88058494834873
2215	9	-1.5430490828019359
2215	10	-3.167714337261767
2215	11	-2.0529441098791636
2215	12	-2.220096411009905
2215	13	-1.7045059665285152
2215	14	-3.0130452001991017
2215	15	-2.6730631971634224
2215	16	-2.3431344355625683
2215	17	-2.7879293410521058
2215	18	-1.506628388687787
2215	19	-2.118445334212204
2215	20	-2.2759466000519453
2215	21	-1.9952073748962391
2215	22	-1.6506635315878952
2215	23	-2.2453600647954985
2215	24	-1.9864084755220617
2215	25	-1.8473680174601412
2216	1	-2.1850101004949387
2216	2	-2.086834472143625
2216	3	-2.6467399273263617
2216	4	-3.45120042328044
2216	5	-2.5910147141974313
2216	6	-2.578257787570653
2216	7	-2.364098991734444
2216	8	-2.9782174862412205
2216	9	-3.2216591989049057
2216	10	-2.736411358022899
2216	11	-3.259113530752117
2216	12	-3.7624943867674787
2216	13	-4.456134058726043
2216	14	-3.639379175921892
2216	15	-3.614825915758974
2216	16	-2.6986750763129876
2216	17	-2.671493640480115
2216	18	-2.755721286102169
2216	19	-3.1526457332703046
2216	20	-3.0403110883377416
2216	21	-3.3128940227918733
2216	22	-3.9768310247489573
2216	23	-3.9574936160955545
2216	24	-3.8079423036951745
2216	25	-3.826344362587246
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94142.0
3	1.0
4	753.0
5	4650.0
6	5847.0
7	5333.0
8	4704.0
9	4596.0
10	4234.0
11	4001.0
12	3750.0
13	3830.0
14	3813.0
15	3942.0
16	3976.0
17	4263.0
18	4922.0
19	5906.0
20	7654.0
21	9787.0
22	12249.0
23	14873.0
24	17241.0
25	19716.0
26	22369.0
27	24879.0
28	28297.0
29	33756.0
30	41207.0
31	52251.0
32	65699.0
33	84373.0
34	109423.0
35	143334.0
36	196343.0
37	304732.0
38	858634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61545631110295	19.10132212215413	12.655999331398993	25.627222235343922
2	14.341186708817775	20.551924736548035	42.07592994898943	23.03095860564476
3	18.39051107944757	25.192301684431	30.04669391421595	26.37049332190548
4	12.020421219342024	17.258248467525668	39.17438582177705	31.546944491355255
5	12.642329156337375	38.29583092788173	36.52125287239221	12.540587043388687
6	30.83598977680443	37.85914044400103	18.75506429332568	12.549805485868868
7	27.075522858568124	31.723194812743255	23.28471336368649	17.916568965002135
8	24.049175682148032	37.88032293667026	21.229500701229256	16.841000679952447
9	26.248584793129687	15.263320146615659	20.63841277730925	37.84968228294541
10	14.454417097736078	29.21851608910835	35.47353806470772	20.853528748447854
11	33.60393071632634	22.430098190952457	25.72009037315821	18.245880719563
12	23.014331021333113	24.77771027779312	32.76850633800713	19.43945236286664
13	28.70365675898346	22.45375861226042	26.005902963251337	22.836681665504784
14	20.232959057189916	22.721634344189788	28.227033898535282	28.81837270008502
15	22.4228889213843	31.939114266687007	23.518069736456695	22.119927075472
16	21.455632568121867	28.281920617251366	28.816728149463632	21.44571866516313
17	21.18808725774062	29.091994734259362	28.470128510871	21.24978949712901
18	21.045499275991368	27.78226822209214	30.63979165949303	20.532440842423462
19	23.05294370628579	27.283913171416284	27.865049005183458	21.798094117114466
20	23.252515613371454	27.87423653375005	28.40049180535662	20.47275604752187
21	22.376742598399034	27.247371332700144	27.41315415629581	22.962731912605015
22	22.686237043656263	28.891158337984645	27.467833501504945	20.954771116854147
23	21.774962030416408	28.430990423290126	28.071145153587246	21.722902392706217
24	22.642718850055747	27.312534035646497	28.819948204705938	21.224798909591815
25	22.200593570677103	27.95879953137251	28.31932400303485	21.521282894915537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	261.0
1	261.0
2	427.0
3	593.0
4	593.0
5	593.0
6	1158.0
7	1723.0
8	1723.0
9	1723.0
10	2737.5
11	3752.0
12	3752.0
13	3752.0
14	6975.0
15	10198.0
16	10198.0
17	10198.0
18	18602.5
19	27007.0
20	27007.0
21	27007.0
22	44248.0
23	61489.0
24	61489.0
25	61489.0
26	88466.5
27	115444.0
28	115444.0
29	115444.0
30	150126.5
31	184809.0
32	184809.0
33	184809.0
34	207923.0
35	231037.0
36	231037.0
37	231037.0
38	246763.5
39	262490.0
40	262490.0
41	262490.0
42	271429.5
43	280369.0
44	280369.0
45	280369.0
46	284213.5
47	288058.0
48	288058.0
49	288058.0
50	272645.0
51	257232.0
52	257232.0
53	257232.0
54	225253.0
55	193274.0
56	193274.0
57	193274.0
58	164086.5
59	134899.0
60	134899.0
61	134899.0
62	108693.0
63	82487.0
64	82487.0
65	82487.0
66	63166.0
67	43845.0
68	43845.0
69	43845.0
70	31492.5
71	19140.0
72	19140.0
73	19140.0
74	13306.5
75	7473.0
76	7473.0
77	7473.0
78	5068.0
79	2663.0
80	2663.0
81	2663.0
82	1768.0
83	873.0
84	873.0
85	873.0
86	572.0
87	271.0
88	271.0
89	271.0
90	171.0
91	71.0
92	71.0
93	71.0
94	41.5
95	12.0
96	12.0
97	12.0
98	11.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35610188822709415
2	0.0415482375943661
3	0.03806325470246393
4	0.03607183590709126
5	0.0432228397632022
6	0.01747017397758749
7	0.01692705976066767
8	0.022720278074479062
9	0.019868928435650017
10	0.019868928435650017
11	0.018873219037963684
12	0.019280554700653547
13	0.019008997592193638
14	0.026884153737531002
15	0.028106160725600595
16	0.020864637833336353
17	0.021905606749099336
18	0.10518312001013813
19	0.023987544580625308
20	0.021543530604486123
21	0.02561688723138476
22	0.0193258142187302
23	0.021498271086409473
24	0.01805854771258396
25	0.021543530604486123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2209480.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.77876145700259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.38459993229897	59.85243307728082
2	11.473232121637674	16.470687831997004
3	2.8387352279264215	6.112826962947614
4	0.997521061190189	2.8640330519802664
5	0.4379442446411656	1.5717547733782706
6	0.23413349199277425	1.0083487242506621
7	0.1267562733490987	0.6368885815532499
8	0.09061201795384526	0.5203214737477355
9	0.06052710250863186	0.3910104407385566
>10	0.31635843304952327	3.9242084581663472
>50	0.02010816354158202	1.0096522603224702
>100	0.015681911777567534	2.2938388338628974
>500	0.002526678755096295	1.3013621099563508
>1k	0.0012633393775471464	2.042633419817797
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4615	0.20887267592374675	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4342	0.19651682748882093	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3956	0.17904665351123342	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3785	0.17130727592012598	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3198	0.14473993880913155	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3104	0.14048554410992634	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2427	0.10984485037203323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.525951807665152E-5
2	0.0	0.0	0.0	0.0	4.525951807665152E-5
3	0.0	0.0	0.0	0.0	4.525951807665152E-5
4	0.0	0.0	0.0	4.525951807665152E-5	4.525951807665152E-5
5	0.0	0.0	0.0	4.525951807665152E-5	4.525951807665152E-5
6	0.0	0.0	0.0	4.525951807665152E-5	4.525951807665152E-5
7	0.0	0.0	0.0	4.525951807665152E-5	4.525951807665152E-5
8	0.0	0.0	0.0	4.525951807665152E-5	4.525951807665152E-5
9	0.0	0.0	0.0	9.051903615330304E-5	4.525951807665152E-5
10	0.0	0.0	0.0	9.051903615330304E-5	4.525951807665152E-5
11	0.0	0.0	0.0	9.051903615330304E-5	4.525951807665152E-5
12	0.0	4.525951807665152E-5	0.0	9.051903615330304E-5	4.525951807665152E-5
13	0.0	4.525951807665152E-5	0.0	1.3577855422995455E-4	4.525951807665152E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	175	0.0	14.118307	18
TAGGACC	885	0.0	12.770997	4
TATTGCG	60	4.0834103E-4	12.670276	16
GGCGAGG	1055	0.0	12.240507	19
GGACCGA	55	0.003081363	12.084498	6
CTTATAC	250	0.0	11.39731	3
ATTGCGA	60	0.0058645336	11.086492	17
CCAACGA	170	1.4006218E-10	10.612498	19
AATGTCC	645	0.0	10.599509	8
AGGACGT	2445	0.0	10.566012	5
TGTACCG	90	9.541117E-5	10.553064	5
TTAGGAC	1560	0.0	10.53277	3
TGGCGAG	2555	0.0	10.451118	18
GGACGTG	2440	0.0	10.428894	6
CGGTTTC	155	3.6452548E-9	10.42256	13
GTCCTAC	2535	0.0	10.404609	1
AGGACCT	1735	0.0	10.401003	5
GGACCTG	1700	0.0	10.221049	6
TGTAGGA	2710	0.0	10.163652	2
GACGTGA	1435	0.0	10.123549	7
>>END_MODULE