FastQCFastQC Report
Thu 26 May 2016
SRR1513009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209480
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43910.1987345438745768No Hit
GTCCTAAAGTGTGTATTTCTCATTT40390.1828031935115955No Hit
CTTTAGGACGTGAAATATGGCGAGG38840.1757879682097145No Hit
GTATCAACGCAGAGTACTTTTTTTT38670.17501855640241143No Hit
CTGTAGGACGTGGAATATGGCAAGA38350.17357025182395858No Hit
GTCCTACAGTGTGCATTTCTCATTT31110.14080236073646288No Hit
TATCAACGCAGAGTACTTTTTTTTT28370.12840125278346037No Hit
GGTATCAACGCAGAGTACTTTTTTT24830.11237938338432571No Hit
CTGTAGGACCTGGAATATGGCGAGA23820.10780817205858394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8650.013.199354
ACCGTGC550.003060607712.0954088
AATCCCG2250.011.81029519
CGTGCGC1104.9633127E-711.22865210
TATGTCG1105.0232484E-711.2182316
AGAGCGT600.00586072911.0874598
GGACCTG15900.011.0057836
GGCGAGG11250.010.96670219
TGTAGGA25900.010.9015832
TGTCGAG1053.5080502E-610.8466818
GTAGGAC24950.010.8218213
AGGACCT17150.010.596365
GGCGTGC1803.45608E-1110.5594848
AGGACGT25250.010.5130875
CTGTAGG26650.010.4887171
AAAGGCG2001.8189894E-1210.4659855
ATGGCAA19450.010.44402417
GGACGTG24950.010.3299686
GTCCTAT3350.010.2171541
TTAGGAC16850.010.2124813
Produced by FastQC (version 0.11.5)