##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513009_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2209480 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.53500823723229 31.0 31.0 34.0 27.0 34.0 2 30.661754802034867 31.0 31.0 34.0 27.0 34.0 3 30.76931449933921 31.0 31.0 34.0 27.0 34.0 4 34.255428426598115 37.0 35.0 37.0 30.0 37.0 5 33.925766243641036 37.0 35.0 37.0 30.0 37.0 6 33.80615257888734 37.0 35.0 37.0 29.0 37.0 7 33.68204690696454 37.0 35.0 37.0 28.0 37.0 8 33.55664273946811 37.0 35.0 37.0 28.0 37.0 9 35.010583033111864 39.0 35.0 39.0 28.0 39.0 10 34.893145445987294 39.0 35.0 39.0 28.0 39.0 11 34.9359681916107 39.0 35.0 39.0 27.0 39.0 12 34.78814698481091 39.0 35.0 39.0 27.0 39.0 13 34.779430454224524 39.0 35.0 39.0 27.0 39.0 14 35.82158381157557 40.0 36.0 41.0 27.0 41.0 15 35.84510427792965 40.0 36.0 41.0 27.0 41.0 16 35.81852155258251 40.0 36.0 41.0 27.0 41.0 17 35.79738897840216 40.0 36.0 41.0 27.0 41.0 18 35.72134348353459 40.0 36.0 41.0 27.0 41.0 19 35.70695548273802 40.0 36.0 41.0 27.0 41.0 20 35.68433477560331 40.0 36.0 41.0 27.0 41.0 21 35.631238119376505 40.0 36.0 41.0 27.0 41.0 22 35.5908132230208 40.0 36.0 41.0 27.0 41.0 23 35.48247913536217 40.0 36.0 41.0 27.0 41.0 24 35.47278183101906 40.0 36.0 41.0 27.0 41.0 25 35.37501991418795 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4975197079385474 1101 2 0.6125822810506456 1101 3 0.6132196727190617 1101 4 0.3675037250088664 1101 5 0.7423311120259797 1101 6 0.6864736852652982 1101 7 0.6405882840760384 1101 8 0.8040820433632945 1101 9 0.47785925866134704 1101 10 0.9857278566197536 1101 11 1.0143709224173918 1101 12 0.9778376646442055 1101 13 1.0936825664884182 1101 14 1.1884940777411401 1101 15 1.1380734732967923 1101 16 1.3269890453846145 1101 17 1.2387386119180164 1101 18 0.7764854887014181 1101 19 1.0726751914816646 1101 20 0.9927002065861146 1101 21 1.0562639579571496 1101 22 0.9908311255889544 1101 23 1.0189261710094897 1101 24 0.9718813054266917 1101 25 1.0591638048976293 1102 1 0.5909131679130368 1102 2 0.5417819637931167 1102 3 0.4343161268631519 1102 4 0.4205798447646245 1102 5 0.49005030672659444 1102 6 0.5582100638298755 1102 7 0.5857406834566845 1102 8 0.5972583362876378 1102 9 0.780176172332709 1102 10 0.857803698671809 1102 11 0.6992537976085273 1102 12 0.7990948492799532 1102 13 0.765285623158583 1102 14 0.9200173066565043 1102 15 0.9190177106693724 1102 16 0.9161015270978652 1102 17 0.8602907447582382 1102 18 0.9289981386907016 1102 19 0.9111278684970969 1102 20 0.9205871708436462 1102 21 0.8281362252803817 1102 22 1.0500385192604824 1102 23 0.8951427612904155 1102 24 0.8067339122926569 1102 25 0.9434392290848663 1103 1 0.5132800914480242 1103 2 0.4659313947448034 1103 3 0.5533722192599093 1103 4 0.509818389341909 1103 5 0.5538008493145696 1103 6 0.6050472981757054 1103 7 0.49145923772546496 1103 8 0.5192502512024575 1103 9 0.6391282153578572 1103 10 0.6696971096445097 1103 11 0.6974767433373188 1103 12 0.6118348401153142 1103 13 0.776565832116404 1103 14 0.9783208280629552 1103 15 0.6571718427358277 1103 16 0.751249667248068 1103 17 0.8799013465455587 1103 18 0.9069772465997303 1103 19 0.9918579121239404 1103 20 0.8752816831168317 1103 21 0.5883448911507259 1103 22 0.7874059721700561 1103 23 0.8238755190736029 1103 24 0.9379563917355611 1103 25 0.9970578273647277 1104 1 0.30827659643721006 1104 2 0.38562857869764144 1104 3 0.37317844218865304 1104 4 0.30459792218328374 1104 5 0.32519849174044424 1104 6 0.4522323903293852 1104 7 0.4283319033899815 1104 8 0.3009673905612402 1104 9 0.44907116927532087 1104 10 0.4821225935342852 1104 11 0.4512098371021196 1104 12 0.44278689714531083 1104 13 0.523766472544466 1104 14 0.6411030510238689 1104 15 0.6673239611547075 1104 16 0.6308574352195606 1104 17 0.5696573123581246 1104 18 0.6774775123277834 1104 19 0.7129508552849444 1104 20 0.7603814433919283 1104 21 0.591646386264749 1104 22 0.6383162834237197 1104 23 0.5272345495046622 1104 24 0.6356825236264783 1104 25 0.7607537125005095 1105 1 0.6069305119221227 1105 2 0.6542933197241041 1105 3 0.5690089264782578 1105 4 0.4853453793189857 1105 5 0.5118824324354776 1105 6 0.5311047231544279 1105 7 0.6184331297899206 1105 8 0.7291935058443499 1105 9 0.8344138576972995 1105 10 0.7382945870468802 1105 11 0.732595578986512 1105 12 0.6660829875811345 1105 13 0.8091346910370092 1105 14 1.0192719215557347 1105 15 0.9250586819517181 1105 16 1.0304350345946958 1105 17 0.7017028614344056 1105 18 0.951802161149125 1105 19 1.0007911036720785 1105 20 0.9778037209842836 1105 21 0.9157563219151115 1105 22 0.8968645690662882 1105 23 0.9493264386276294 1105 24 0.9996186272033611 1105 25 1.1035527153104638 1106 1 0.43723842117376677 1106 2 0.4723500207663278 1106 3 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2216 13 -2.1597709713728435 2216 14 -2.233767728787022 2216 15 -2.3285030253624086 2216 16 -1.8833071224362996 2216 17 -2.207011098773826 2216 18 -1.980559084435754 2216 19 -2.176988892583381 2216 20 -2.6703822832746837 2216 21 -2.4323365491640843 2216 22 -2.7732251000291868 2216 23 -1.8334832810834385 2216 24 -2.0803958020499493 2216 25 -1.902677576224093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51558.0 3 0.0 4 802.0 5 7376.0 6 8602.0 7 6877.0 8 5573.0 9 4870.0 10 4346.0 11 3956.0 12 3704.0 13 3363.0 14 3417.0 15 3436.0 16 3543.0 17 3666.0 18 4167.0 19 4791.0 20 6235.0 21 8213.0 22 10331.0 23 12700.0 24 15073.0 25 17220.0 26 20040.0 27 22102.0 28 25421.0 29 30460.0 30 38710.0 31 49521.0 32 63157.0 33 81378.0 34 106432.0 35 138222.0 36 190023.0 37 295930.0 38 954265.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.01801037511386 19.92781899308039 13.660898738689445 27.393271893116307 2 13.223406816819915 20.98339153717895 40.81881857960892 24.97438306639221 3 16.788022521136195 25.36040154244438 30.571401415717723 27.280174520701706 4 11.203070219917134 17.8816225045635 38.48056348332513 32.43474379219425 5 12.303353916072608 38.46973304641602 36.6234010934684 12.60351194404297 6 30.910602100762308 38.367357106945015 18.299958781938336 12.42208201035434 7 26.880181538047786 31.88053695346956 23.182548151998272 18.056733356484383 8 24.164258734227666 38.09604253661718 20.837054683466334 16.90264404568882 9 25.9497436649795 15.02672929916982 20.560097227089916 38.463429808760765 10 14.181498386566227 29.529870499398758 35.464914498430964 20.82371661560405 11 33.60466195190948 22.464407355021216 25.580039603960397 18.350891089108913 12 22.75597066473295 24.986216842339687 32.6272916479949 19.630520844932462 13 28.67087541935145 22.37204915079634 26.029255478047077 22.927819951805134 14 20.17822500998035 22.666069807209567 28.1194129292794 29.03629225353069 15 22.309644164581577 32.0026922115966 23.552551535846064 22.135112087975752 16 21.404124393601336 28.45213247273045 28.58806716975632 21.555675963911895 17 21.060968714108057 29.186418318365764 28.427438796099576 21.325174171426607 18 20.941967621645134 27.898665679387392 30.505979119586463 20.65338757938101 19 22.98694450793536 27.278125657696723 27.922504373329595 21.81242546103833 20 23.13786282967543 27.948193376392368 28.344595970797105 20.5693478231351 21 22.224294108009417 27.17384318620631 27.613676377273507 22.988186328510768 22 22.5711946434237 28.705739971241982 27.610047834979508 21.11301755035481 23 21.544571573401885 28.326529319115814 28.27045277621884 21.858446331263465 24 22.551651316894166 27.389895989054438 28.80742473678184 21.251027957269564 25 22.089085039175494 27.826028314256657 28.40097636616356 21.683910280404287 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 127.0 1 127.0 2 296.5 3 466.0 4 466.0 5 466.0 6 1136.0 7 1806.0 8 1806.0 9 1806.0 10 2804.0 11 3802.0 12 3802.0 13 3802.0 14 6919.5 15 10037.0 16 10037.0 17 10037.0 18 18855.0 19 27673.0 20 27673.0 21 27673.0 22 45119.5 23 62566.0 24 62566.0 25 62566.0 26 90010.5 27 117455.0 28 117455.0 29 117455.0 30 150893.5 31 184332.0 32 184332.0 33 184332.0 34 207903.5 35 231475.0 36 231475.0 37 231475.0 38 247049.0 39 262623.0 40 262623.0 41 262623.0 42 271689.0 43 280755.0 44 280755.0 45 280755.0 46 284266.5 47 287778.0 48 287778.0 49 287778.0 50 272810.0 51 257842.0 52 257842.0 53 257842.0 54 225292.5 55 192743.0 56 192743.0 57 192743.0 58 163224.5 59 133706.0 60 133706.0 61 133706.0 62 107663.0 63 81620.0 64 81620.0 65 81620.0 66 62303.0 67 42986.0 68 42986.0 69 42986.0 70 30929.0 71 18872.0 72 18872.0 73 18872.0 74 13063.0 75 7254.0 76 7254.0 77 7254.0 78 4872.5 79 2491.0 80 2491.0 81 2491.0 82 1654.5 83 818.0 84 818.0 85 818.0 86 512.5 87 207.0 88 207.0 89 207.0 90 123.0 91 39.0 92 39.0 93 39.0 94 21.5 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07680540217607763 2 1.8103807230660608E-4 3 0.0 4 0.029328167713670184 5 0.07436138819993846 6 0.0773937759110741 7 0.03607183590709126 8 0.06788927711497728 9 0.04716041783587088 10 0.10798921013089054 11 0.004752249398048409 12 0.011857993736082699 13 0.005250104096891577 14 0.006245813494577909 15 0.006517370603037818 16 0.014980900483371654 17 0.026838894219454353 18 0.0018556402411427123 19 0.002941868674982349 20 0.004978546988431667 21 2.715571084599091E-4 22 4.0733566268986364E-4 23 0.0 24 4.525951807665152E-5 25 0.0028513496388290456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2209480.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.06408075016522 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8569957038227 58.88156233412548 2 11.791934798471592 16.75966013438537 3 2.9586937091547885 6.3077054598714515 4 1.030903771864242 2.930409155576416 5 0.43646699091825614 1.5508562743698293 6 0.25135730148879204 1.0717485342085882 7 0.1482733160745688 0.737583483463255 8 0.09719343914976282 0.5525569926500011 9 0.06168546632497123 0.39452588640264175 >10 0.32610902549914633 4.00543039026897 >50 0.01984768183320725 0.9717051021014259 >100 0.017033962050477375 2.476105744699329 >500 0.0019117272804185452 0.994595493322379 >1k 0.0015931060670149118 2.3655550145548614 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4391 0.1987345438745768 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4039 0.1828031935115955 No Hit CTTTAGGACGTGAAATATGGCGAGG 3884 0.1757879682097145 No Hit GTATCAACGCAGAGTACTTTTTTTT 3867 0.17501855640241143 No Hit CTGTAGGACGTGGAATATGGCAAGA 3835 0.17357025182395858 No Hit GTCCTACAGTGTGCATTTCTCATTT 3111 0.14080236073646288 No Hit TATCAACGCAGAGTACTTTTTTTTT 2837 0.12840125278346037 No Hit GGTATCAACGCAGAGTACTTTTTTT 2483 0.11237938338432571 No Hit CTGTAGGACCTGGAATATGGCGAGA 2382 0.10780817205858394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 4.525951807665152E-5 0.0 13 0.0 0.0 0.0 4.525951807665152E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 865 0.0 13.19935 4 ACCGTGC 55 0.0030606077 12.095408 8 AATCCCG 225 0.0 11.810295 19 CGTGCGC 110 4.9633127E-7 11.228652 10 TATGTCG 110 5.0232484E-7 11.21823 16 AGAGCGT 60 0.005860729 11.087459 8 GGACCTG 1590 0.0 11.005783 6 GGCGAGG 1125 0.0 10.966702 19 TGTAGGA 2590 0.0 10.901583 2 TGTCGAG 105 3.5080502E-6 10.84668 18 GTAGGAC 2495 0.0 10.821821 3 AGGACCT 1715 0.0 10.59636 5 GGCGTGC 180 3.45608E-11 10.559484 8 AGGACGT 2525 0.0 10.513087 5 CTGTAGG 2665 0.0 10.488717 1 AAAGGCG 200 1.8189894E-12 10.465985 5 ATGGCAA 1945 0.0 10.444024 17 GGACGTG 2495 0.0 10.329968 6 GTCCTAT 335 0.0 10.217154 1 TTAGGAC 1685 0.0 10.212481 3 >>END_MODULE