FastQCFastQC Report
Thu 26 May 2016
SRR1513008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042408
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28090.13753373468964086No Hit
GTATCAACGCAGAGTACTTTTTTTT27710.13567318576895507No Hit
GTCCTAAAGTGTGTATTTCTCATTT25750.12607667028331263No Hit
CTGTAGGACGTGGAATATGGCAAGA24940.1221107633734298No Hit
CTTTAGGACGTGAAATATGGCGAGG21500.10526789945985326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6500.012.2749294
TGTAGGA17150.010.8556452
GTCCTAA7850.010.800171
ACTGTTC4550.010.642458
GTCCTAC16850.010.62840751
AGGACGT15200.010.5607845
CCGTCCA909.571147E-510.54970559
GTAGGAC16750.010.4908333
GTCTTAC1752.237357E-1010.3425061
TGGCGAG16050.010.30168318
GGCGAGG7250.010.21477519
CTGTAGG17500.010.1792051
AATCCCG1409.77343E-810.17268219
GGACGTG15650.010.1315146
TCCTACA18150.010.0482012
GACGTGG7750.010.0457977
GCCGGTT951.6536201E-49.99445711
GTGTAGG3250.09.9656541
TAGGACG17000.09.8336964
CCTACAG18150.09.7862883
Produced by FastQC (version 0.11.5)