##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513008_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042408 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.199484138330835 31.0 30.0 34.0 23.0 34.0 2 29.218791250328046 31.0 30.0 34.0 23.0 34.0 3 29.443823663048715 31.0 30.0 34.0 23.0 34.0 4 32.897225725712005 35.0 35.0 37.0 26.0 37.0 5 33.034079870427455 37.0 35.0 37.0 26.0 37.0 6 33.01878958562638 37.0 35.0 37.0 26.0 37.0 7 32.9069936075456 37.0 35.0 37.0 26.0 37.0 8 32.89159707560879 37.0 35.0 37.0 26.0 37.0 9 34.23944040563883 39.0 35.0 39.0 25.0 39.0 10 34.115455384036885 39.0 35.0 39.0 25.0 39.0 11 34.18032587024727 39.0 35.0 39.0 25.0 39.0 12 34.07966625669308 39.0 35.0 39.0 25.0 39.0 13 34.07389806542081 39.0 35.0 39.0 25.0 39.0 14 35.08992620475439 40.0 35.0 41.0 25.0 41.0 15 35.02501508023862 40.0 35.0 41.0 24.0 41.0 16 34.912101303951026 39.0 35.0 41.0 22.0 41.0 17 34.84989580926044 39.0 34.0 41.0 22.0 41.0 18 34.557374432532576 39.0 34.0 41.0 18.0 41.0 19 34.79035628532595 39.0 34.0 41.0 19.0 41.0 20 34.86708679166944 39.0 35.0 41.0 22.0 41.0 21 34.808448654725204 39.0 35.0 41.0 21.0 41.0 22 34.71823896106948 39.0 35.0 41.0 19.0 41.0 23 34.45473676170481 39.0 34.0 41.0 18.0 41.0 24 34.453748222686166 39.0 34.0 41.0 18.0 41.0 25 34.37052048366438 39.0 34.0 41.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.447589023914265 1101 2 0.7008764106384113 1101 3 0.7159228815593188 1101 4 0.6969852459370287 1101 5 0.48646352504864865 1101 6 0.6864493938943852 1101 7 0.6214192211903367 1101 8 0.48375363420634443 1101 9 0.3258898947543898 1101 10 0.42417879313915563 1101 11 0.6091637851450997 1101 12 0.7827267295367619 1101 13 0.6324187613319481 1101 14 0.7517118290556724 1101 15 0.4916369514761101 1101 16 0.9978318607411651 1101 17 0.9171372634016706 1101 18 1.1483748151511577 1101 19 0.6457293770334687 1101 20 0.7915276890543268 1101 21 0.8102428655526808 1101 22 0.766639249432707 1101 23 0.9792524823313045 1101 24 0.6313877286684999 1101 25 0.9245636095805381 1102 1 0.7974637892418137 1102 2 0.8076405022128483 1102 3 0.8936728868562085 1102 4 0.9546791417212219 1102 5 0.9073495480644169 1102 6 0.8275040739060344 1102 7 1.0637026476255684 1102 8 1.0455781604946779 1102 9 1.1644416351194522 1102 10 1.162830702994519 1102 11 1.1875456256462016 1102 12 1.309309559040507 1102 13 1.232119945837944 1102 14 1.3168033952833582 1102 15 1.48899398134985 1102 16 1.3463476826241774 1102 17 1.3298455996706764 1102 18 1.5146657794900733 1102 19 1.5517551684722974 1102 20 1.5006685782889306 1102 21 1.2730234777432656 1102 22 1.4915883866278108 1102 23 1.6801490808771078 1102 24 1.6018882648961252 1102 25 1.3788982944906678 1103 1 0.5007892355263763 1103 2 0.5216243737313455 1103 3 0.6512455935385155 1103 4 0.7404380923376905 1103 5 0.20640557761584688 1103 6 0.5709725535470085 1103 7 0.4824416336667383 1103 8 0.5052380243004819 1103 9 0.6770901190126395 1103 10 0.5905380882994109 1103 11 0.7515122720693412 1103 12 0.8585698583609016 1103 13 0.8625181248797276 1103 14 1.0868437248036855 1103 15 1.0087953071685263 1103 16 1.1054821443308924 1103 17 0.8282967108776731 1103 18 1.1821252604007384 1103 19 1.166331698724619 1103 20 1.1289344852315395 1103 21 0.8954427343138249 1103 22 0.9249612188023519 1103 23 1.309153793244434 1103 24 1.1128776409113001 1103 25 1.050095537927497 1104 1 0.41044109292345254 1104 2 0.38637532081309445 1104 3 0.4651378310413534 1104 4 0.6410764182103463 1104 5 0.41319244683167966 1104 6 0.3238218042189729 1104 7 0.6497465871551711 1104 8 0.5278967183371392 1104 9 0.7163254283601361 1104 10 0.866186713844705 1104 11 0.7147097356358358 1104 12 0.7091038402514442 1104 13 0.6804053662115024 1104 14 0.8388879413471315 1104 15 0.897634308543509 1104 16 0.7583584767961256 1104 17 0.7122235006093192 1104 18 0.9343335883157664 1104 19 0.9074487924323122 1104 20 0.8904832006329357 1104 21 1.0198066441047047 1104 22 0.8795372217952107 1104 23 1.2037820892861149 1104 24 0.9566430294032386 1104 25 0.6119881745304454 1105 1 0.6204363926348329 1105 2 0.7658591436627127 1105 3 0.8010449284771717 1105 4 1.0298003869631387 1105 5 0.6099471111883403 1105 6 0.518472021170318 1105 7 0.7832557604870587 1105 8 0.7011264381455504 1105 9 0.559438145942714 1105 10 0.7910327891037028 1105 11 0.7755314703919467 1105 12 0.905535311208709 1105 13 0.8373637356386467 1105 14 0.8665986677165236 1105 15 1.1150675236574585 1105 16 1.0554600302220933 1105 17 0.9645654873941254 1105 18 0.9463397181136486 1105 19 1.0244049865192721 1105 20 1.0703599443040517 1105 21 1.095070889669536 1105 22 0.7062838063168897 1105 23 0.9044197358787969 1105 24 1.1491660744133583 1105 25 1.2261533294651628 1106 1 0.7979893662278705 1106 2 0.7939372946062164 1106 3 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1107 16 0.9668676242232195 1107 17 0.9905005963566182 1107 18 1.3232963857733395 1107 19 1.2179715047349973 1107 20 1.0976795688652103 1107 21 1.0940478225800163 1107 22 0.9154804742782545 1107 23 1.0617929460770341 1107 24 1.0133939849506106 1107 25 1.0322968228263534 1108 1 0.37434200425582986 1108 2 0.47729150143838694 1108 3 0.40183361537037143 1108 4 0.5685770501729834 1108 5 0.5398099062112749 1108 6 0.6625656853224129 1108 7 0.6217984718636771 1108 8 0.5999777232691343 1108 9 0.7809437037206663 1108 10 0.7520360139989108 1108 11 0.6299360781434515 1108 12 0.6905646689407803 1108 13 0.7092603190697773 1108 14 0.9106477786426836 1108 15 0.9190244188853356 1108 16 0.8095042308139924 1108 17 0.7903811432081369 1108 18 0.9904151817989799 1108 19 0.9146218564560797 1108 20 0.8428069022666662 1108 21 0.8219825548416182 1108 22 0.7586418094981795 1108 23 0.8531467459402364 1108 24 0.9942619017667624 1108 25 1.0406818520612333 1109 1 0.002145072463541453 1109 2 -0.07089723867683517 1109 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17 -2.991631546609767 2215 18 -1.891302994781995 2215 19 -2.503959369695309 2215 20 -2.747775732374862 2215 21 -2.521974916011672 2215 22 -2.030284784585767 2215 23 -2.466647548255178 2215 24 -2.489403437507047 2215 25 -2.364307956889622 2216 1 -2.3883716068040854 2216 2 -2.4173063657704965 2216 3 -2.9476841185836555 2216 4 -3.733038892846892 2216 5 -2.7231207097059844 2216 6 -2.650939943922314 2216 7 -2.606201892783755 2216 8 -3.1017390923282626 2216 9 -3.3932515451611174 2216 10 -2.9141552237611243 2216 11 -3.5803473757811695 2216 12 -3.915957169278631 2216 13 -4.349996870330873 2216 14 -3.8861972283629065 2216 15 -3.8584784200138245 2216 16 -2.978045635713162 2216 17 -3.117167256802169 2216 18 -3.094626346390843 2216 19 -3.531211353016033 2216 20 -3.3855700004893983 2216 21 -3.7216954694034747 2216 22 -4.302635407766292 2216 23 -4.060448459738748 2216 24 -4.069456867865242 2216 25 -3.977793287505591 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92497.0 3 4.0 4 752.0 5 4487.0 6 5701.0 7 5100.0 8 4491.0 9 4361.0 10 4135.0 11 3774.0 12 3607.0 13 3663.0 14 3611.0 15 3767.0 16 3773.0 17 4069.0 18 4606.0 19 5564.0 20 7060.0 21 9188.0 22 11511.0 23 13928.0 24 16180.0 25 18311.0 26 20444.0 27 23078.0 28 25938.0 29 30549.0 30 37535.0 31 47396.0 32 59744.0 33 76629.0 34 99620.0 35 131290.0 36 179023.0 37 277246.0 38 799776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4768872311896 18.4029516262885 12.338782547512132 25.78137859500977 2 14.911906033101976 20.20454846025363 40.92345596771113 23.96008953893327 3 18.534527727341967 24.822729078308758 29.74962885393131 26.893114340417966 4 12.303830452106492 16.77346675385041 38.460717110789474 32.461985683253616 5 13.142813198310682 37.82405779183692 35.927640685876405 13.105488323975992 6 31.696538755362287 37.11665785195197 18.30571389394919 12.881089498736559 7 27.713743259807224 31.45348207993865 22.728338951078847 18.10443570917528 8 24.90000847240567 36.75324681684863 20.79907028815484 17.547674422590863 9 26.507174643471565 15.036706965728902 20.03170447550249 38.42441391529704 10 14.732034638606581 28.687330405156906 35.07298485454694 21.50765010168957 11 34.332101217620234 22.25526146709918 24.811509802077946 18.601127513202638 12 23.11046860408002 24.709880161664955 31.917956945172897 20.26169428908213 13 28.927487231874256 21.843497474778392 25.71558556101154 23.513429732335815 14 21.057474979736465 21.889222737279272 27.65185408294949 29.401448200034775 15 23.1730855409667 30.374269584394685 23.38671879017561 23.06592608446301 16 22.42006911908669 27.82496230379979 27.477848738211826 22.277119838901697 17 21.92575724185215 28.274590464996695 27.61140086681848 22.188251426332673 18 21.877426109050415 27.413218814451177 29.188640516315218 21.520714560183187 19 23.521322381399212 27.00098731960358 27.321817216581483 22.15587308241572 20 23.694372386381836 27.19622282437025 27.71528573926185 21.394119049986067 21 23.196277930823385 26.715102540557083 27.042546678910316 23.046072849709212 22 23.291810337017466 27.71531453706438 26.970701777325278 22.022173348592876 23 22.592591322931874 27.518503083071778 27.305472152449834 22.583433441546514 24 23.195294437977502 26.90086086790019 27.807321123462238 22.09652357066007 25 22.922945374871322 27.15599861701096 27.532353814042825 22.38870219407489 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 225.0 2 375.0 3 525.0 4 525.0 5 525.0 6 1019.0 7 1513.0 8 1513.0 9 1513.0 10 2319.0 11 3125.0 12 3125.0 13 3125.0 14 5728.0 15 8331.0 16 8331.0 17 8331.0 18 15372.5 19 22414.0 20 22414.0 21 22414.0 22 36139.0 23 49864.0 24 49864.0 25 49864.0 26 71246.5 27 92629.0 28 92629.0 29 92629.0 30 120071.5 31 147514.0 32 147514.0 33 147514.0 34 168828.5 35 190143.0 36 190143.0 37 190143.0 38 208408.0 39 226673.0 40 226673.0 41 226673.0 42 239699.5 43 252726.0 44 252726.0 45 252726.0 46 260228.5 47 267731.0 48 267731.0 49 267731.0 50 259571.0 51 251411.0 52 251411.0 53 251411.0 54 226196.0 55 200981.0 56 200981.0 57 200981.0 58 173541.0 59 146101.0 60 146101.0 61 146101.0 62 119340.5 63 92580.0 64 92580.0 65 92580.0 66 71500.0 67 50420.0 68 50420.0 69 50420.0 70 36905.0 71 23390.0 72 23390.0 73 23390.0 74 16247.0 75 9104.0 76 9104.0 77 9104.0 78 6239.5 79 3375.0 80 3375.0 81 3375.0 82 2261.0 83 1147.0 84 1147.0 85 1147.0 86 749.5 87 352.0 88 352.0 89 352.0 90 224.0 91 96.0 92 96.0 93 96.0 94 58.0 95 20.0 96 20.0 97 20.0 98 19.0 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3520844023329325 2 0.04102999988249165 3 0.037162016600013316 4 0.0363786275807772 5 0.04279262517577291 6 0.0180179474424307 7 0.017968985628728444 8 0.023746479645594808 9 0.020808770823459367 10 0.020025381804223252 11 0.019193030971284875 12 0.019927458176818735 13 0.020025381804223252 14 0.02756550111437088 15 0.028936431898034087 16 0.020808770823459367 17 0.02242451067563386 18 0.10301565602954944 19 0.025264295870364786 20 0.022473472489336118 21 0.02609664670330316 22 0.019927458176818735 23 0.022081777979718057 24 0.019535763667200678 25 0.022228663420824833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2042408.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.08655051174526 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52121293944393 59.48669584849712 2 11.853471664397043 17.089517677501973 3 3.0658204537904488 6.630132630063209 4 1.0983744223028844 3.167120930965836 5 0.48066366954620504 1.732469294695167 6 0.2689914443804519 1.1634399205535257 7 0.1648554834805927 0.8318704195943353 8 0.10634444135464079 0.6132803154683717 9 0.07428184371628108 0.4819249691243337 >10 0.33241145530043564 4.016815329075153 >50 0.018863741844525463 0.9344374441159585 >100 0.011850182041295992 1.7137265513246198 >500 0.0019055056008527602 0.8927505021181571 >1k 9.527528004262437E-4 1.2458181669022979 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2809 0.13753373468964086 No Hit GTATCAACGCAGAGTACTTTTTTTT 2771 0.13567318576895507 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2575 0.12607667028331263 No Hit CTGTAGGACGTGGAATATGGCAAGA 2494 0.1221107633734298 No Hit CTTTAGGACGTGAAATATGGCGAGG 2150 0.10526789945985326 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.8961813702257336E-5 0.0 2 0.0 0.0 0.0 4.8961813702257336E-5 0.0 3 0.0 0.0 0.0 4.8961813702257336E-5 0.0 4 0.0 0.0 0.0 4.8961813702257336E-5 0.0 5 0.0 0.0 0.0 4.8961813702257336E-5 0.0 6 0.0 0.0 0.0 4.8961813702257336E-5 0.0 7 0.0 0.0 0.0 4.8961813702257336E-5 0.0 8 0.0 0.0 0.0 9.792362740451467E-5 0.0 9 0.0 0.0 0.0 1.9584725480902934E-4 0.0 10 0.0 0.0 0.0 2.4480906851128667E-4 0.0 11 0.0 0.0 0.0 2.4480906851128667E-4 0.0 12 0.0 0.0 0.0 2.4480906851128667E-4 0.0 13 0.0 0.0 0.0 2.4480906851128667E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 650 0.0 12.274929 4 TGTAGGA 1715 0.0 10.855645 2 GTCCTAA 785 0.0 10.80017 1 ACTGTTC 455 0.0 10.64245 8 GTCCTAC 1685 0.0 10.6284075 1 AGGACGT 1520 0.0 10.560784 5 CCGTCCA 90 9.571147E-5 10.5497055 9 GTAGGAC 1675 0.0 10.490833 3 GTCTTAC 175 2.237357E-10 10.342506 1 TGGCGAG 1605 0.0 10.301683 18 GGCGAGG 725 0.0 10.214775 19 CTGTAGG 1750 0.0 10.179205 1 AATCCCG 140 9.77343E-8 10.172682 19 GGACGTG 1565 0.0 10.131514 6 TCCTACA 1815 0.0 10.048201 2 GACGTGG 775 0.0 10.045797 7 GCCGGTT 95 1.6536201E-4 9.994457 11 GTGTAGG 325 0.0 9.965654 1 TAGGACG 1700 0.0 9.833696 4 CCTACAG 1815 0.0 9.786288 3 >>END_MODULE